共查询到20条相似文献,搜索用时 15 毫秒
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Haixiang Wei Honglin Teng Weipeng Huan Shuangwei Zhang Hongran Fu Fangyi Chen Jing Wang Chunlei Wu Jian Zhao 《Neurochemical research》2012,37(12):2758-2766
SENP3 (SUMO-specific proteases 3), a member of the small ubiquitin-like modifier specific protease family, was identified as a molecule that deconjugates SUMOylation of modified protein substrates and functions as an isopeptidase by disrupting SUMO homeostasis to facilitate cancer development and progression. However, its expression and function in nervous system injury and repair are still unclear. In this study, we employed an acute spinal cord injury (SCI) model in adult rats and investigated the dynamic changes of SENP3 expression in the spinal cord. Western blot analysis indicated a gradual increase in SENP3 expression, which peaked 3?days after SCI, and then declined over the following days. Immunohistochemistry results further confirmed that SENP3 was expressed at low levels in the gray and white matter in the non-injured condition and increased after SCI. Moreover, immunofluorescence double-labeling showed that SENP3 was co-expressed with the neuronal marker, NeuN. Furthermore, the SENP3-positive cells that were co-expressed with NeuN had also expressed active caspase-3 after injury. To investigate whether SENP3 plays a role in neuronal apoptosis, we applied H2O2 to induce neuronal apoptosis in vitro. Western blot analysis showed a significant upregulation of SENP3 and active caspase-3 following H2O2 stimulation. Taken together, these results suggest that SENP3 may play important roles in the pathophysiology of SCI. 相似文献
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Zhou Yulong Zheng Binbin Ye Libing Zhang Hongyu Zhu Sipin Zheng Xiaomeng Xia Qinghai He Zili Wang Qingqing Xiao Jian Xu Huazi 《Neurochemical research》2021,46(2):423-423
Neurochemical Research - This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1007/s11064-020-03149-1 . 相似文献
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Locomotor Dysfunction and Pain: The Scylla and Charybdis of Fiber Sprouting After Spinal Cord Injury 总被引:1,自引:0,他引:1
Injury to the spinal cord (SCI) can produce a constellation of problems including chronic pain, autonomic dysreflexia, and motor dysfunction. Neuroplasticity in the form of fiber sprouting or the lack thereof is an important phenomenon that can contribute to the deleterious effects of SCI. Aberrant sprouting of primary afferent fibers and synaptogenesis within incorrect dorsal horn laminae leads to the development and maintenance of chronic pain as well as autonomic dysreflexia. At the same time, interruption of connections between supraspinal motor control centers and spinal cord output cells, due to lack of successful regenerative sprouting of injured descending fiber tracts, contributes to motor deficits. Similarities in the molecular control of axonal growth of motor and sensory fibers have made the development of cogent therapies difficult. In this study, we discuss recent findings related to the degradation of inhibitory barriers and promotion of sprouting of motor fibers as a strategy for the restoration of motor function and note that this may induce primary afferent fiber sprouting that can contribute to chronic pain. We highlight the importance of careful attentiveness to off-target molecular- and circuit-level modulation of nociceptive processing while moving forward with the development of therapies that will restore motor function after SCI. 相似文献
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To demonstrate calpain involvement in neurodegeneration in rat spinal cord injury (SCI), we examined SCI segments for DNA
fragmentation, neurons for calpain overexpression, neuronal death, and neuroprotection with calpain inhibitor (E-64-d). After
the induction of SCI (40 g cm force) on T12, rats were treated within 15 min with vehicle (DMSO) or E-64-d. Sham animals underwent
laminectomy only. Animals were sacrificed at 24 h, and five 1-cm long spinal cord segments were collected: two rostral (S1
and S2), one lesion (S3), and two caudal segments (S4 and S5). Agarose gel electrophoresis of DNA samples isolated from the
SCI segments showed both random and internucleosomal DNA fragmentation indicating occurrence of necrosis as well as apoptosis
mostly in the lesion, moderately in caudal, and slightly in rostral segments from SCI rats. Treatment of SCI rats with E-64-d
(1 mg/kg) reduced DNA fragmentation in all segments. The lesion and adjacent caudal segments (S3 and S4) were further investigated
by in situ double-immunofluorescent labelings that showed increase in calpain expression in neurons in SCI rats and decrease
in calpain expression in SCI rats treated with E-64-d. In situ combined TUNEL and double-immunofluorescent labelings directly
detected co-localization of neuronal death and calpain overexpressin in SCI rats treated with only vehicle while attenuation
of neuronal death in SCI rats treated with E-64-d. Previous studies from our laboratory indirectly showed neuroprotective
effect of E-64-d in SCI rats. Our current results provide direct in situ evidence for calpain involvement in neuronal death
and neuroprotective efficacy of E-64-d in lesion and penumbra in SCI rats.
Special issue in honor of Naren Banik. 相似文献
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Andie M. Thompkins Sarah J. Adkins Maggie Leopard Cameron Spencer David Bentley Lauren Bolden 《Anthrozo?s》2013,26(5):679-690
ABSTRACTApproximately 17,000 new cases of spinal cord injury (SCI) are reported annually in the United States. Rehabilitation from SCI involves substantial mental, emotional, and physical challenges. Using a randomized controlled trial design, we assessed the efficacy of animal-assisted therapy (AAT) as an aid in rehabilitation following a SCI. We hypothesized that patients with SCI undergoing rehabilitation occupational therapy with AAT would demonstrate greater positive shifts in mood and outlook, reduced pain, and reduced stress compared with patients exposed to the same rehabilitation therapy but without AAT. Over four sessions of occupational therapy, 31 patients completed standard rehabilitation activities (control group) or rehabilitation activities integrating an animal therapy team (treatment group). Patients completed the Positive and Negative Affect Schedule (PANAS) and Numerical Rating Scale (NRS) at each session, had salivary cortisol sampled at the second session, and completed the Brief Pain Inventory at study baseline and exit. Data were analyzed using repeated measures ANOVAs and t-tests. The results revealed a small but significant effect of animal-assisted therapy on self-reported negative affect. Findings for group differences on positive affect, stress, and pain unpleasantness were null, although non-significant findings were in the hypothesized direction for several variables and yielded small effect sizes. Continued research is needed on the influence of AAT on mood improvement, stress reduction, and ultimately improved physical health outcomes during rehabilitation after SCI. 相似文献
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Tian Wei Han Xiao-Guang Liu Ya-Jun Tang Guo-Qing Liu Bo Wang Yong-Qing Xiao Bin Xu Yun-Feng 《Neurochemical research》2020,45(7):1729-1730
Neurochemical Research - Since the publication of our article [1] it has come to our attention that there was an error in Figure 4 in which the bottom left immunochemistry panel Control/Bax was a... 相似文献
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Peter E. Batchelor Taryn E. Wills Peta Skeers Camila R. Battistuzzo Malcolm R. Macleod David W. Howells Emily S. Sena 《PloS one》2013,8(8)
Background
The use of early decompression in the management of acute spinal cord injury (SCI) remains contentious despite many pre-clinical studies demonstrating benefits and a small number of supportive clinical studies. Although the pre-clinical literature favours the concept of early decompression, translation is hindered by uncertainties regarding overall treatment efficacy and timing of decompression.Methods
We performed meta-analysis to examine the pre-clinical literature on acute decompression of the injured spinal cord. Three databases were utilised; PubMed, ISI Web of Science and Embase. Our inclusion criteria consisted of (i) the reporting of efficacy of decompression at various time intervals (ii) number of animals and (iii) the mean outcome and variance in each group. Random effects meta-analysis was used and the impact of study design characteristics assessed with meta-regression.Results
Overall, decompression improved behavioural outcome by 35.1% (95%CI 27.4-42.8; I2=94%, p<0.001). Measures to minimise bias were not routinely reported with blinding associated with a smaller but still significant benefit. Publication bias likely also contributed to an overestimation of efficacy. Meta-regression demonstrated a number of factors affecting outcome, notably compressive pressure and duration (adjusted r2=0.204, p<0.002), with increased pressure and longer durations of compression associated with smaller treatment effects. Plotting the compressive pressure against the duration of compression resulting in paraplegia in individual studies revealed a power law relationship; high compressive forces quickly resulted in paraplegia, while low compressive forces accompanying canal narrowing resulted in paresis over many hours.Conclusion
These data suggest early decompression improves neurobehavioural deficits in animal models of SCI. Although much of the literature had limited internal validity, benefit was maintained across high quality studies. The close relationship of compressive pressure to the rate of development of severe neurological injury suggests that pressure local to the site of injury might be a useful parameter determining the urgency of decompression. 相似文献11.
Samantaray S Smith JA Das A Matzelle DD Varma AK Ray SK Banik NL 《Neurochemical research》2011,36(10):1809-1816
Spinal cord injury (SCI), depending on the severity of injury, leads to neurological dysfunction and paralysis. Methylprednisolone,
the only currently available therapy renders limited protection in SCI. Therefore, other therapeutic agents must be tested
to maximize neuroprotection and functional recovery. Previous data from our laboratory indicate that estrogen (17β-estradiol)
at a high dose may attenuate multiple damaging pathways involved in SCI and improve locomotor outcome. Since use of high dose
estrogen may have detrimental side effects and therefore may never be used in the clinic, the current study investigated the
efficacy of this steroid hormone at very low doses in SCI. In particular, we tested the impact of dosing (1–10 μg/kg), mode
of delivery (intravenous vs. osmotic pump), and delay in estrogen application (15 min–4 h post-SCI) on microgliosis and neuronal
death in acute SCI in rats. Treatment with 17β-estradiol (1–10 μg/kg) significantly reduced microglial activation and also
attenuated apoptosis of neurons compared to untreated SCI animals. The attenuation of cell death and inflammation by estrogen
was observed regardless of mode and time of delivery following injury. These findings suggest estrogen as a potential agent
for the treatment of individuals with SCI. 相似文献
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Rüdiger Rupp Daniel Schlie?mann Harry Plewa Christian Schuld Hans Jürgen Gerner Norbert Weidner Eberhard P. Hofer Markus Knestel 《PloS one》2015,10(3)
Background
The compact Motorized orthosis for home rehabilitation of Gait (MoreGait) was developed for continuation of locomotion training at home. MoreGait generates afferent stimuli of walking with the user in a semi-supine position and provides feedback about deviations from the reference walking pattern.Objective
Prospective, pre-post intervention, proof-of-concept study to test the feasibility of an unsupervised home-based application of five MoreGait prototypes in subjects with incomplete spinal cord injury (iSCI).Methods
Twenty-five (5 tetraplegia, 20 paraplegia) participants with chronic (mean time since injury: 5.8 ± 5.4 (standard deviation, SD) years) sensorimotor iSCI (7 ASIA Impairment Scale (AIS) C, 18 AIS D; Walking Index for Spinal Cord Injury (WISCI II): Interquartile range 9 to 16) completed the training (45 minutes / day, at least 4 days / week, 8 weeks). Baseline status was documented 4 and 2 weeks before and at training onset. Training effects were assessed after 4 and 8 weeks of therapy.Results
After therapy, 9 of 25 study participants improved with respect to the dependency on walking aids assessed by the WISCI II. For all individuals, the short-distance walking velocity measured by the 10-Meter Walk Test showed significant improvements compared to baseline (100%) for both self-selected (Mean 139.4% ± 35.5% (SD)) and maximum (Mean 143.1% ± 40.6% (SD)) speed conditions as well as the endurance estimated with the six-minute walk test (Mean 166.6% ± 72.1% (SD)). One device-related adverse event (pressure sore on the big toe) occurred in over 800 training sessions.Conclusions
Home-based robotic locomotion training with MoreGait is feasible and safe. The magnitude of functional improvements achieved by MoreGait in individuals with iSCI is well within the range of complex locomotion robots used in hospitals. Thus, unsupervised MoreGait training potentially represents an option to prolong effective training aiming at recovery of locomotor function beyond in-patient rehabilitation.Trial Registration
German Clinical Trials Register (DKRS) DRKS00005587 相似文献14.
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SYNOPSIS. The lamprey, a lower vertebrate, has recently becomea very useful model system for studying motor control, includingthe organization of neural networks, and for examining the relationbetween kinematic patterns and underlying neural mechanisms.This aquatic animal displays a number of interesting locomotorbehaviors, including flexure reflexes, forward locomotion, backwardlocomotion, turning, withdrawal, and equilibrium reflexes. Avaluable property of the lamprey preparation is that the nervoussystem survives under in vitro conditions for several days andgenerates well-coordinated locomotor activity that underliessome of the above behaviors. Thus, the neural control of basiclocomotor behaviors can be studied in an isolated nervous systemin which the ionic or pharmacological make-up of the bath canbe altered and in which stable conditions are provided for intracellularrecordings. In addition, recent data indicate that the lampreypreparation is a valuable model system for studies of axonalregeneration and recovery of locomotor function following spinalcord injury. This article will focus on three aspects of locomotorbehavior in the lamprey: kinematics and motor activity of locomotorbehaviors, descending control of locomotion, and regenerationof descending brainstem command pathways that initiate locomotion. 相似文献
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Zinc plays an important role in regulating the expression of brain-derived neurotrophic factor (BDNF) and its receptor in
nervous system, but the correlation among Zn2+, zinc transporter, and BDNF in spinal cord injuries (SCI) is not fully understood. The purpose of this study was to investigate
the expression of Zn2+, zinc transporter 1 (ZnT-1), and BDNF, as well as their correlation in spinal cord-injured rats. One hundred Wistar male
rats were divided into two groups: sham-operated group (as control group) and model group. Spinal cord injury was induced
in model groups by hemisection of T9 on the left side. Compared with the control group, the serum zinc levels in SCI model
group were significantly decreased after surgery, but zinc concentrations in spinal cord were increased gradually. The mRNA
levels of ZnT-1 and BDNF were significantly increased in SCI model group, and there is a positive correlation between them
(Spearman rho = 0.381, P = 0.0204). The correlation found between BDNF and ZnT-1 allows us to speculate that these two factors may be physiologically
co-regulated, which may provide an idea for the treatment of SCI. 相似文献
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Rui Yang Lan Guo Peng Wang Lin Huang Yong Tang Wenhao Wang Keng Chen Jichao Ye Ciyong Lu Yanfeng Wu Huiyong Shen 《PloS one》2014,9(1)
Background
Spinal cord injuries are highly disabling and deadly injuries. Currently, few studies focus on non-traumatic spinal cord injuries, and there is little information regarding the risk factors for complete injuries. This study aims to describe the demographics and the injury characteristics for both traumatic and non-traumatic spinal cord injuries and to explore the risk factors for complete spinal cord injuries.Methods
A retrospective study was performed by reviewing the medical records of 3,832 patients with spinal cord injuries who were first admitted to the sampled hospitals in Guangdong, China. The demographics and injury characteristics of the patients were described and compared between the different groups using the chi-square test. Logistic regression was conducted to analyze the risk factors for complete spinal cord injuries.Results
The proportion of patients increased from 7.0% to 14.0% from 2003 to 2011. The male-to-female ratio was 3.0∶1. The major cause of spinal cord injuries was traffic accidents (21.7%). Many of the injured were workers (36.2%), peasants (22.8%), and unemployed people (13.9%); these occupations accounted for 72.9% of the total sample. A multivariate logistic regression model revealed that the OR (95% CI) for male gender compared to female gender was 1.25 (1.07–1.89), the OR (95%CI) for having a spinal fracture was 1.56 (1.35–2.60), the OR (95%CI) for having a thoracic injury was 1.23 (1.10–2.00), and the OR (95%CI) for having complications was 2.47 (1.96–3.13).Conclusion
The proportion of males was higher than the proportion of females. Workers, peasants and the unemployed comprised the high-risk occupational categories. Male gender, having a spinal fracture, having a thoracic injury, and having complications were the major risk factors for a complete injury. We recommend that preventive measures should focus on high-risk populations, such as young males. 相似文献19.
Olivier Alluin Hugo Delivet-Mongrain Marie-Krystel Gauthier Michael G. Fehlings Serge Rossignol Soheila Karimi-Abdolrezaee 《PloS one》2014,9(10)
While several cellular and pharmacological treatments have been evaluated following spinal cord injury (SCI) in animal models, it is increasingly recognized that approaches to address the glial scar, including the use of chondroitinase ABC (ChABC), can facilitate neuroanatomical plasticity. Moreover, increasing evidence suggests that combinatorial strategies are key to unlocking the plasticity that is enabled by ChABC. Given this, we evaluated the anatomical and functional consequences of ChABC in a combinatorial approach that also included growth factor (EGF, FGF2 and PDGF-AA) treatments and daily treadmill training on the recovery of hindlimb locomotion in rats with mid thoracic clip compression SCI. Using quantitative neuroanatomical and kinematic assessments, we demonstrate that the combined therapy significantly enhanced the neuroanatomical plasticity of major descending spinal tracts such as corticospinal and serotonergic-spinal pathways. Additionally, the pharmacological treatment attenuated chronic astrogliosis and inflammation at and adjacent to the lesion with the modest synergistic effects of treadmill training. We also observed a trend for earlier recovery of locomotion accompanied by an improvement of the overall angular excursions in rats treated with ChABC and growth factors in the first 4 weeks after SCI. At the end of the 7-week recovery period, rats from all groups exhibited an impressive spontaneous recovery of the kinematic parameters during locomotion on treadmill. However, although the combinatorial treatment led to clear chronic neuroanatomical plasticity, these structural changes did not translate to an additional long-term improvement of locomotor parameters studied including hindlimb-forelimb coupling. These findings demonstrate the beneficial effects of combined ChABC, growth factors and locomotor training on the plasticity of the injured spinal cord and the potential to induce earlier neurobehavioral recovery. However, additional approaches such as stem cell therapies or a more adapted treadmill training protocol may be required to optimize this repair strategy in order to induce sustained functional locomotor improvement. 相似文献
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Fehlings MG Vaccaro A Wilson JR Singh A W Cadotte D Harrop JS Aarabi B Shaffrey C Dvorak M Fisher C Arnold P Massicotte EM Lewis S Rampersaud R 《PloS one》2012,7(2):e32037