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1.
Synaptonemal complex analysis in a human male carrier of a 4;6 translocation: heterosynapsis without previous homosynapsis 总被引:1,自引:1,他引:0
Summary Silver-stained synaptonemal complexes in surface-spread pachytene nuclei from a man, heterozygous for a reciprocal translocation, were analysed by electron microscopy. Contrary to the classically expected cross-shaped configuration, extensive non-homologous pairings were observed with asymetrical association in the lateral elements of the non-homologous arms of the quadrivalents. A possible role of the heterosynapsis in reproductive failure is discussed. 相似文献
2.
H Jaafar A Bouvet O Gabriel-Robez M Bonneau J Boscher J L Maetz C P Popescu Y Rumpler 《Annales de génétique》1992,35(1):3-7
The authors report here two new cases of reciprocal translocations in two fertile and hypoprolific boars. Silver stained synaptonemal complexes in surface-spread pachytene nuclei from a boar heterozygous for a reciprocal translocation, and from another one carrying two different reciprocal translocations, were analyzed by electron microscopy. In such heterozygotes, cross-shaped quadrivalent configurations are expected to form in order to allow homologous pairing. In the same boar, the lengths of the fully synapsed arms of the quadrivalent varies from one quadrivalent to the other and heterosynapsis was obvious. Heterosynapsis was also observed with asymmetrical pairing of the non-homologous partners of the quadrivalent. This heterosynapsis is assumed to be a mechanism preventing spermatocyte loss, but inducing a secondary segregational type of impairment of fertility due to foetal wastage leading to reduced prolificacy. 相似文献
3.
Villagómez DA Ayala-Valdovinos MA Galindo-García J Sánchez-Chipres DR Mora-Galindo J Taylor-Preciado JJ 《Cytogenetic and genome research》2008,120(1-2):112-116
Due to its low fertility, expressed as small litter size, a Mexican hairless boar was subjected to cytogenetic investigation. Analysis of G-banded mitotic chromosomes revealed a reciprocal chromosome translocation, rcp(3;6) (p14;q21). Synaptonemal complex analysis showed a regular pairing behavior of the translocation chromosome axes, always resulting in a quadrivalent configuration. However, due to extensive nonhomologous pairing between the axes of nonderivative chromosomes 3 and 6, the quadrivalent mostly had an asymmetrical cross-shaped morphology. The nonhomologous pairing occurred not only at mid and late pachytene, but also at the earliest stage of pachytene. It seems that early pachytene heterosynapsis is a common phenomenon in the pairing behavior of pig reciprocal translocations. Therefore, heterosynapsis may reduce apoptosis of germ cells due to partial absence of homologous synapsis during the pairing phase of meiosis. The frequency of spermatocytes showing quadrivalent configurations with unpaired axial segments apparently did not affect germ cell progression in the boar, since fairly normal testicular histology was noticed. 相似文献
4.
Summary Silver-stained synaptonemal complexes in surface-spread pachytene nuclei from an oligospermic man, heterozygous for a reciprocal translocation involving an acrocentric chromosome, were analyzed by electron microscopy. Contrary to the classically expected configuration, nonhomologous pairing was observed with asymetrical association of the lateral elements of the nonhomologous arms of the quadrivalents. A possible role of heterosynapsis in germ cell conservation is discussed. 相似文献
5.
A cytological analysis of the pairing configurations in meiosis in a 19-week human fetus with a de novo paracentric inversion of chromosome 7 (q11.23)(q21.1) is reported, using fluorescent in situ hybridization with a chromosome 7 DNA library, a DNA probe for the centromeric region of chromosome 7, and a probe for the William Syndrome Critical Region (WSCR) at 7q11.23. Of 1079 pachytene cells, 58% exhibited complete heterosynapsis of the inverted region while only 10.3% of cells exhibited the expected loop formation. Meiotic progression was observed to be normal. 相似文献
6.
Synapsis of a chromosomal pair heterozygous for a pericentric inversion and the presence of a heterochromatic short arm 总被引:1,自引:0,他引:1
Analysis of meiotic pairing configurations in a deer mouse heterozygous for both a pericentric inversion and the presence of a heterochromatic short arm at chromosome 15 revealed straight-paired synaptonemal complexes with equal axial lengths in a majority of the pachytene nuclei. Nonhomologous pairing in this bivalent occurs by direct heterosynapsis of the inverted segments followed by synaptic adjustment of the heterochromatin heteromorphism. 相似文献
7.
Correlation between chromosomal breakpoint positions and synaptic behaviour in human males heterozygous for a pericentric inversion 总被引:4,自引:1,他引:3
Summary The examination of synaptic data and localization of chromosomal breakpoints in a review of human pericentric inversions suggest that synaptic and recombination behaviour in rearranged chromosomes during meiosis can be predicted by determining the subband in which the breakpoint is located. According to this hypothesis, it can be postulated that loops in pericentric inversions are routinely formed only in cases when both breaks occur in G-light bands, whith the genetic consequences of crossing-over. In other cases, heterosynapsis is accomplished without previous homosynapsis, thereby minimizing the production of unbalanced gametes. 相似文献
8.
David W. Hale 《Chromosoma》1986,94(6):425-432
The patterns of chromosomal pairing and chiasma distribution were analyzed in male Sitka deer mice (Peromyscus sitkensis) polymorphic for terminally positioned pericentric inversions of chromosomes 6 and 7. Gand C-banding of somatic metaphases indicated that the inversions involved 30% and 40% of chromosomes 6 and 7, respectively. Analysis of silver-stained synaptonemal complexes in surface-spread zygotene and pachytene nuclei from heterozygous individuals revealed that inversion loops were not formed. The inverted segments proceeded directly to heterosynapsis without an intervening homosynaptic phase, and the heteromorphic bivalents remained straight-paired throughout pachynema. C-banded pachytene nuclei corroborated the occurrence of heterosynapsis, as the heteromorphic bivalents exhibited nonaligned centromeres. Analysis of diplonema and diakinesis indicated that crossing over had not occurred within the heterosynapsed inverted segments. The observation of chiasma suppression within the inversions indicates that pericentric inversion heterozygosity does not lead to the production of unbalanced gametes. Heterosynapsis of the inverted segments during zygonema and pachynema and the resulting chiasma suppression therefore represent a meiotic mechanism for the maintenance of pericentric inversion polymorphisms in this population of P. sitkensis. 相似文献
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10.
We propose a feasible multipath switch based on the optical bistability with surface plasmon. The switch is controlled by comparing the intensities of TE and TM modes scattering light from the cross-shaped silver structure involving Kerr material in the center. The working wavelength of the switch has a blue shift by reducing the length of a pair of nanorods in the cross-shaped structure due to the scattering of the structure changed. Our structure is easy to implement in practice and has potential applications in all-optical network in the future. 相似文献
11.
A new species of Acanthodasys (Gastrotricha, Macrodasyida, Thaumastodermatidae) is described from sublittoral sediments off the Atlantic coast of Florida. Acanthodasys paurocactussp. n. is a relatively small species (to 450 μm long) with a strap-shaped outline, a series of anterior, lateral, and ventrolateral adhesive tubes, paired caudal pedicles with posterior adhesive tubes, and a morphologically diverse cuticle. The cuticle contains both spined and unspined scales. Unspined scales are present in two general shapes: lanceolate and eye shaped, with some transitional shapes. All scales have a thickened rim and depressed central region; some scales of both shapes bear either one or more central bumps, a parallel ridge, or a perpendicular ridge that gives the appearance of a cross-shaped pattern under transmitted light. Spined scales are somewhat quadrangular in shape and bear uniancres to 15 μm long with a cross-shaped sectional profile. The new species is now one of five described species to possess both spined and spineless scales, and only one of two species to possess two types of spineless scales (the second species is an incompletely described specimen from Norway). 相似文献
12.
There is an inverse relationship between the effectiveness of the examining by rats of the cross-shaped labyrinth and their individual sensitivity to gamma-irradiation with doses inducing a cerebral form of radiation sickness. 相似文献
13.
Plasmonic properties of cross-shaped Ag nanoparticles are investigated theoretically using finite-difference time-domain algorithm.
Electric field (E-field) distribution of a single cross-shaped Ag nanoparticle with different shape parameters and patterned
nanoparticles with different periods were presented. Both red shift and blue shift of the extinction spectra were observed.
The simulation results demonstrated that the strong E-field intensity is located at sharp corner of the nanoparticles. And
E-field intensity of the nanoparticle array is much stronger than that of a single Ag nanoparticle. Enhancement of the large
localized E-field originating from the nanoparticles was analyzed. Corresponding influence of “hot spots” effect on enhancing
Raman scattering was discussed as well. 相似文献
14.
Analysis of surface-spread synaptonemal complexes of zygotene and pachytene spermatocytes was carried out on centric-shift heterozygotes of grasshoppers. These rearrangements affected the M7 chromosome in Chorthippus vagans and the M6 and S8 chromosomes in Chorthippus apricarius. The shifts in the latter two chromosomes were also associated with C-heterochromatin variations between homologous chromosomes. Rearranged chromosomes proceeded directly to heterosynapsis without an apparent intervening homosynaptic phase in M7 bivalents of Ch. vagans and M6 bivalents of Ch. apricarius. In the latter case, axial equalization of the heterochromatin polymorphism was also achieved. On the other hand, asynapsis of the intercentromeric regions throughout pachytene was the rule in the centric shift involving the S8 chromosome of Ch. apricarius. In the three cases analysed, the production of unbalanced gametes in the heterozygotes is precluded either by the lack of chiasma formation in heterosynapsed rearranged segments or by the lack of pairing between such segments. Chiasmata were limited to the homologous regions of the heteromorphic bivalents. 相似文献
15.
Eight different sized supernumerary segments located at distal ends of the long arms of chromosomes M4, M5, M6, and S8 of the grasshopper Stenobothrus festivus were studied in males with regard to the synaptic process and chiasma distribution in the bivalents that carry them. The M4, M5, and M6 bivalents heterozygous for extra segments were always monochiasmate, in contrast to their bichiasmate condition observed in basic homozygotes. Furthermore, the presence of any of these extra segments led to chiasma redistribution in the carrier bivalents, so that such chiasmata were formed preferentially further away from the extra segment. The intensity of this effect is dependent on the size of the segment. Not all heteromorphic bivalents exhibited synaptonemal complexes with equalized axes at pachytene, but there was always a variable proportion of heterosynapsis around the distal ends of the long arms that was dependent on both the size of the segment and the size of the carrier chromosome. It is proposed that the absence of chiasmata in nonhomologous synapsed regions is responsible for the results obtained. Length measurements of the different extra segments and their carrier chromosomes between pachytene and diplotene indicated that synaptonemal complex is underrepresented in supernumerary heterochromatin. 相似文献
16.
Two paracentric inversions in the mouse, In(1)1 Rk and In(2)5 Rk, have been studied in surface microspreads of spermatocytes from heterozygotes. At zytogene, synaptic initiation occurs independently in three regions: within the inversion, and without, on either side. Synaptonemal complex (SC) formation is restricted to homologous regions, resulting in inversion loops in all early pachytene spermatocytes. An adjusting phase then occurs during pachytene in which the inversion loop is reduced by desynapsis of homologously synapsed SC, followed immediately by non-homologous synapsis with the alternate pairing partner, progressing from the ends toward the middle. Adjustment occurs during the first half of pachytene, but is not closely synchronized with sub-stage. It is complete by late pachytene, the loop having been eliminated in all cases and replaced by straight SCs in which the inverted region is heterosynapsed. Synapsis in the adjustment phase is evidently permitted only after the homosynaptic phase, and is indifferent to homology. It may lead to heterosynapsis, as in the inversion region, or to synapsis of homologous regions not synapsed at zytogene. The anaphase bridge frequency, a measure of crossing over within the inversion, is about 34% for both inversions studied, indicating that such crossovers do not block adjustment, that crossing over probably occurs before or during the adjustment period, and that there is some crossover suppression. The last could be the consequence of blocking by desynapsis/heterosynapsis. Synaptic adjustment appears to be a general phenomenon that occurs to varying extents in different forms. A hypothetical scheme for two phases of synapsis is proposed: at zytogene, a basic propensity for indifferent SC formation is limited by a restricting condition to synapsis between homologous regions. Subsequently, the restriction is lifted, whereupon synaptic instability is resolved by desynapsis, followed by resynapsis that is indifferent to homology, but that results in a topologically more stable structure. 相似文献
17.
The use of cell-based biosensors is usually limited by agonist-induced desensitization of cell-surface receptors. In this work, a microfluidic cell-based biosensor (μCBB) was developed for the detection of ATP in liquid environments. It consists of a millisecond chemical pulse generator for sample introduction in a pulsatile manner and a single NIH-3T3 cell expressing endogenous P2Y receptors as the sensing element. ATP solutions were used to simulate input signals for investigating the μCBB. By controlling negative pressures on two outlets of a cross-shaped microfluidic chip, pulses of ATP solutions were generated based on hydrodynamic gated injection. With ATP pulses of 100 ms every 50s, the amplitude of the resulting calcium spikes maintained at a similar level, suggesting that the receptor desensitization was minimized. Consequently, the developed μCBB could be used for detecting pulsatile samples with extended use times. The sensitivity of the μCBB for detecting ATP was further determined and the cellular responses to millisecond ATP pulses were investigated in comparison to long-term stimulations. 相似文献
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19.
Identification of molecules in leech extracellular matrix that promote neurite outgrowth 总被引:3,自引:0,他引:3
L Masuda-Nakagawa K Beck M Chiquet 《Proceedings of the Royal Society of London. Series B, Containing papers of a Biological character. Royal Society (Great Britain)》1988,235(1280):247-257
The molecular composition of the substrate is of critical importance for neurite extension by isolated identified leech nerve cells in culture. One substrate upon which rapid growth occurs in defined medium is a cell-free extract of extracellular matrix (ECM) that surrounds the leech central nervous system (CNS). Here we report the co-purification of neurite-promoting activity with a laminin-like molecule. High molecular mass proteins from leech ECM purified by gel filtration exhibited increased specific activity for promoting neurite outgrowth. The most active fractions contained three major polypeptide bands of ca. 340, 250 and 220 kDa. Electron microscopy of rotary-shadowed samples showed three macromolecules, one of which had a cross-shaped structure similar to vertebrate laminin. A second six-armed molecule resembled vertebrate tenascin and a third rod-like molecule resembled vertebrate collagen type IV. The most active fractions contained a protein of ca. 1 MDa on non-reducing gels with disulphide-linked subunits of ca. 220 and 340 kDa, with cross-shaped laminin-like molecules. We conclude that a laminin-like molecule represents a major neurite promoting component present in leech ECM. The experiments represent a first step in determining the location of leech laminin within the CNS and assessing its role in neurite outgrowth during development and regeneration. 相似文献