Importance of species diversity in the revegetation of Alberta’s northern fescue prairies

Restoration of grassland ecosystems is critical to the provision of ecosystem services, however, legacies of historic disturbances pose a challenge to grassland restoration. In the northern Great Plains of North America, continued fragmentation and disturbance of northern fescue prairies has prompted more stringent criteria to regulate the revegetation of native prairies disturbed by industrial activities. Here, we evaluate methods of revegetating northern fescue prairies, disturbed by energy development, and test the hypothesis that higher richness of species seeded within disturbed areas improves the structure, diversity, and composition of revegetated communities. Our results demonstrate that disturbed northern fescue prairies are able to recover their structural elements, including vegetative and ground cover and plant litter, irrespective of the number of species in the seed mixes, even though revegetated areas remained similar in all measures of community diversity. Despite this, revegetated areas remained compositionally different from adjacent native prairies, 7 years following seeding treatments. Based on our observations, the persistent differences in the species composition of disturbed and undisturbed prairies highlight that all efforts should be practiced to minimize the scale of disturbance of northern fescue prairies through energy development.     

Temporal dynamics of plant community regeneration sources during tallgrass prairie restoration

Ecological restoration aims to augment and steer the composition and contribution of propagules for community regeneration in degraded environments. We quantified patterns in the abundance, richness, and diversity of seed and bud banks across an 11-year chronosequence of restored prairies and in prairie remnants to elucidate the degree to which the germinable seed bank, emerged seedlings, belowground buds, and emerged ramets were related to community regeneration. There were no directional patterns in the abundance, richness, or diversity of the germinable seed bank across the chronosequence. Emerged seedling abundance of sown species decreased during restoration. Richness and diversity of all emerged seedlings and non-sown emerged seedling species decreased across the chronosequence. Conversely, abundance and richness of belowground buds increased with restoration age and belowground bud diversity of sown species increased across the chronosequence. Numbers of emerged ramets also increased across the chronosequence and was driven primarily by the number of graminoid ramets. There were no temporal changes in abundance and richness of sown and non-sown emerged ramets, but diversity of sown emerged ramets increased across the chronosequence. This study demonstrates that after initial seeding, plant community structure in restored prairies increasingly reflects the composition of the bud bank.     

Inoculation with remnant prairie soils increased the growth of three native prairie legumes but not necessarily their associations with beneficial soil microbes

Restoring the diversity of plant species found in remnant communities is a challenge for restoration practitioners, in part because many reintroduced plant species fail to establish in restored sites. Legumes establish particularly poorly, perhaps because they depend on two guilds of soil microbial mutualists, rhizobial bacteria and arbuscular mycorrhizal (AM) fungi, that may be absent from restored sites. We tested the effect of soil microorganisms from remnant and restored prairies on legume growth by inoculating seedlings of Lespedeza capitata, Amorpha canescens, and Dalea purpurea with soil from 10 restored prairies and 6 remnant (untilled) prairies from southwest Michigan. We generally found support for the hypothesis that restored prairie soils lack microbes that enhance prairie plant growth, although there was variation across species and mutualist guilds. All three legumes grew larger and two legumes (Lespedeza and Amorpha) produced more nodules when inoculated with soil from remnant prairies, suggesting that low quantity and/or quality of rhizobial partners may limit the establishment of those species in restored prairies. In contrast, no legume experienced greater root colonization by AM fungi in remnant prairie soils, suggesting equivalent quantity (but not necessarily quality) of fungal partners in remnant and restored prairie soils. We detected no evidence of spontaneous recovery of the community of beneficial soil microbes in restorations. These results suggest that the absence of rhizobia, a largely overlooked component of prairie soils, could play a strong role in limiting restored prairie diversity by hindering legume establishment. Active reintroduction of appropriate rhizobial strains could enhance prairie restoration outcomes.     

Legacies of afforestation on soil nematode community composition,structure, and diversity in a northern Canadian prairie

Plant and Soil - We examined how legacies of afforestation affect soil food webs using the composition, structure, and diversity of soil nematode communities along a prairie restoration...     

Incomplete recovery of plant diversity in restored prairie wetlands on agricultural landscapes

Restoration efforts are being implemented globally to mitigate the degradation and loss of wetland habitat; however, the rate and success of wetland vegetation recovery post‐restoration is highly variable across wetland classes and geographies. Here, we measured the recovery of plant diversity along a chronosequence of restored temporary and seasonal prairie wetlands ranging from 0 to 23 years since restoration, including drained and natural wetlands embedded in agricultural and natural reserve landscapes in central Alberta, Canada. We assessed plant diversity using the following structural indicators: percent cover of hydrophytes, native and non‐native species, species richness, and community composition. Our findings indicate that plant diversity recovered to resemble reference wetlands in agricultural landscapes within 3–5 years of restoration; however, restored wetlands maintained significantly lower species richness and a distinct community composition compared to reference wetlands located within natural reserves. Early establishment of non‐native species during recovery, dispersal limitation, and depauperated native seed bank were probable barriers to complete recovery. Determining the success of vegetation recovery provides important knowledge that can be used to improve restoration strategies, especially considering projected future changes in land use and climate.     

Bee communities (Hymenoptera: Apoidea) of small Iowa hill prairies are as diverse and rich as those of large prairie preserves

Natural small, xeric hill prairies in forested landscapes throughout the Midwest United States often contain high diversity and unique species of some organisms because of their unusual landscape context and microclimate. We measured the diversity, richness, and abundance of the bee communities of five hill prairies located in northeastern Iowa and we compared these to values for large prairie preserves in northwestern Iowa, using a Monte Carlo resampling approach to standardize sampling effort between habitat types. We also measured the diversity and richness of the flowering forb communities at the hill prairies and we quantified percentage of the landscape at a 1 km radius in different landscape elements. Bee diversity at the five hill prairies spanned the range of diversity values for large prairies preserves, so although the hill prairies are small (<5 ha), their bee communities are not uniformly depauparate compared to larger western prairie preserves. Bee diversity was significantly related to flowering forb diversity, and may have been influenced by landscape features within 1 km—particularly the percentage of agricultural row crops and open water, which may negatively affect bee diversity at the sites. Iowa’s hill prairie bee communities were largely composed of widespread eastern species, although about 10% of the bee species have more northern or western ranges and appear to be taking advantage of the region’s unique habitat features. Given the dependence of the bee communities on the plant diversity of the sites, management of the plant community to maintain its diversity will also likely benefit bee diversity.     

Patterns of Plant Species Diversity in Remnant and Restored Tallgrass Prairies

To restore diversity of native vegetation, we must understand factors responsible for diversity in targeted communities. These factors operate at different spatial scales and may affect the number and relative abundances of species differently. We measured diversity of plant species and functional groups of species in replicated plots within paired restored and remnant (relic) tallgrass prairies at three locations in central Texas, U.S.A. To determine the contributions of species abundances and of spatial patterns of diversity to differences between prairie types, we separated diversity into richness and evenness (relative biomass) and into within‐plot (α), among‐plot (β), and prairie (γ) components. Species diversity was greater in remnant than in restored prairies at all spatial scales. At the γ scale, both species richness and species evenness were greater in remnants because of greater spatial variation in species composition. At the α scale, remnants were more diverse because of greater richness alone. Mean α richness correlated positively with the size of the species pool in restored prairies only, implying that in remnants, α richness was influenced more by colonization dynamics than by the number of species available for colonization. Plots in remnant prairies contained more functional groups and fewer species per group than did plots in restored prairies, suggesting that resource partitioning was greater in relic prairies. Our results are consistent with the interpretation that local ecological processes, like resource partitioning and limitations on seed dispersal, contribute to the greater diversity of remnant than restored prairies in central Texas. Restoration practices that limit abundances of competitive dominants, increase the number of species in seed mixtures, and increase the proximity of plants of different functional groups thus may be required to better simulate the plant diversity of tallgrass prairies.     

Invasion or retreat? The fate of exotic invaders on the northern prairies, 40?years after cattle grazing

Despite the detrimental impacts of invasive plants on native biodiversity, ecosystem function, and management cost, few studies have focused on the long-term persistence of invaders. Here, we use a unique, long-term dataset to examine the recovery of northern rough fescue prairie communities, 41?years after the removal of livestock from Riding Mountain National Park, Manitoba, Canada. Our 1973 data suggest that summer grazing of plains?? rough fescue (Festuca hallii (Vasey) Piper) leads to its displacement from the plant community and increased dominance by exotic invaders. After 41?years of recovery time, historic grazing intensity remained an excellent predictor of community structure and composition. Areas classified as heavily grazed in 1973 remained characterized by exotic grasses and had significantly lower richness and diversity. While some exotic invaders persisted despite 41?years of community recovery, others were ephemeral. For example, both Poa pratensis (L.) and Bromus inermis (Leyss.) persisted, increasing in abundance across all classes of grazing intensity, suggesting that their control requires active restoration of the invaded areas. In contrast, passive restoration may be possible for a subset of ephemeral exotic species such as Taraxacum officinale, which had virtually disappeared from invaded prairies by 2010. Our long-term data provide a rare perspective into the long-term dynamics of plant invasions. Based on our findings, conservation managers will need to consider the dichotomy between persistent and ephemeral invaders and their impact on the recovery of northern prairie communities as they focus their restoration efforts against the mounting impacts of exotic plant invaders.     

Rapid Shift in Pollinator Communities Following Invasive Species Removal

Ecological restoration is increasingly used to reverse degradation of rare ecosystems and maintain biological diversity. Pollinator communities are critical to maintenance of plant diversity and, in light of recent pollinator loss, we tested whether removal of invasive glossy buckthorn (Frangula alnus L.) from portions of a prairie fen wetland altered plant and pollinator communities. We compared herbaceous plant, bee, and butterfly abundance, diversity, and species composition in buckthorn invaded, buckthorn removal, and uninvaded reference plots. Following restoration, we found striking differences in plant and pollinator abundance and species composition between restored, unrestored, and reference plots. Within 2 years of F. alnus removal, plant species diversity and composition in restored plots were significantly different than invaded plots, but also remained significantly lower than reference plots. In contrast, in the first growing season following restoration, bee and butterfly abundance, diversity, and composition were similar in restored and reference plots and distinct from invaded plots. Our findings indicate that a diverse community of mobile generalist pollinators rapidly re‐colonizes restored areas of prairie fen, while the plant community may take longer to fully recover. This work implies that, in areas with intact pollinator metapopulations, restoration efforts will likely prevent further loss of mobile generalist pollinators and maintain pollination services. On the other hand, targeted restoration efforts will likely be required to restore populations of rare plants and specialist pollinators for which local and regional species pools may be lacking.     

High Plant Richness in Prairie Reconstructions Support Diverse Leafhopper Communities

A primary reason for restoring plant communities is to increase biodiversity to previous levels. It is expected that restoring land with greater plant diversity will increase biodiversity at higher trophic levels, but high diversity seed mixes are expensive. In this study, we used one insect family, leafhoppers (Hemiptera: Cicadellidae) to assess the difference in leafhopper communities that result from establishing high compared with low plant richness restorations. We tested the hypotheses that: (1) the added effort of a high richness restoration leads to measurable increases in both diversity and richness of leafhoppers; and (2) that leafhopper community composition is more similar to remnant prairies in high richness than in low plant richness restorations. We found that higher plant richness led to 3‐ to 7‐fold increases in leafhopper and prairie‐dependent leafhopper diversity and richness in restorations. Leafhopper communities in high richness restorations were not more similar to remnant prairies, rather they were distinct among high and low richness restorations and prairie interior. Leafhopper richness and diversity correlated with plant richness, and leafhopper community composition differed among plant community assemblages, but not with the occurrence of single plant species. For our sites, species‐rich restorations provided better quality habitat for leafhoppers that was comparable to remnant prairie. Our results suggest that restorations with high plant species richness better support animal food webs.     

Dominant species constrain effects of species diversity on temporal variability in biomass production of tallgrass prairie

Species diversity is thought to stabilize functioning of plant communities. An alternative view is that stability depends more on dynamics of dominant species than on diversity. We compared inter-annual variability (inverse of stability) of aboveground biomass in paired restored and remnant tallgrass prairies at two locations in central Texas, USA. Data from these two locations were used to test the hypothesis that greater richness and evenness in remnant than restored prairies would reduce variability in aboveground biomass in response to natural variation in rainfall. Restored prairies were chosen to be similar to paired remnant prairies in characteristics other than species diversity that affect temporal variability in biomass. Variability was measured as the coefficient of variation among years (square root of variance/mean; CV), where variance in community biomass equals the sum of variances of individual plant species plus the summed covariances between species pairs. Species diversity over five years was greater by a factor of 2 or more in remnant than restored prairies because richness and evenness were greater in remnant than restored prairies. Still, the CV of community biomass during spring and CV of annual biomass production did not differ consistently between prairie types. Neither the sum of species covariances nor total community biomass differed between prairies. Biomass varied relatively little in restored compared to remnant prairies because biomass of the dominant species in restored prairies (the grass Schizachyrium scoparium ) varied less than did biomass of other dominant and sub-dominant species. In these grasslands, biomass response to natural variation in precipitation depended as much on characteristics of a dominant grass as on differences in diversity.     

Dispersal and establishment filters influence the assembly of restored prairie plant communities

Community assembly filters, which in theory determine the suite of species that arrive at and establish in a community, have tremendous conceptual relevance to restoration. However, the concept has remained largely theoretical, with a paucity of empirical tests. As such, the applicability of assembly filters theory to ecological restoration remains incompletely known. We tested the relative strengths of dispersal and establishment filters by comparing the plant species composition, measured by species' presence/absence, in 29 restored prairies with the seed mixes used to restore each prairie. We found that both establishment and dispersal filters limited prairie similarity to the seed mix. Sown species responded differentially to filters, with a few species limited only by dispersal (seed density), many others limited only by establishment conditions (i.e. organic matter and sand content of soils, land use history, and fire frequency), and others limited by both dispersal and establishment filters. A few species, typically those sown most often, were not restricted by dispersal or establishment filters, likely because they were sown in high enough densities and all sites had suitable environmental conditions. Finally, one group of species established poorly, but we could not attribute this to either dispersal or establishment filters. This information can help land managers select species likely to establish in restorations when sown at sufficient densities. These results illustrate that dispersal and establishment filters limit the establishment of species in restored communities and these filters are species‐dependent. Identifying the most limiting filter(s) for species will inform strategies to increase their establishment success.     

Plant–microbe interactions change along a tallgrass prairie restoration chronosequence

Soil microbial communities are critical in determining the performance and density of species in plant communities. However, their role in regulating the success of restorations is much less clear. This study assessed the ability of soil microbial communities to regulate the growth and performance of two potentially dominant grasses and two common forbs in tallgrass prairie restorations. Specifically, we examined the effects of soil microbial communities along a restoration chronosequence from agricultural fields to remnant prairies using experimentally inoculated soils. The two grass species, Andropogon gerardii and Sorghastrum nutans, grew best with the agricultural inoculates and experienced a decline in performance in later stages of the chronosequence, indicating that the microbial community shifted from being beneficial to grasses in the early stages to inhibiting grasses in the later stages of restoration. Growth of the forb, Silphium terebinthinaceum, varied little with inoculation or position along the restoration chronosequence. Growth of Baptisia leucantha, a legume, appeared limited by nodule formation in agricultural soils, peaked in young restoration soils along with nodule formation, but decreased in older soils as the microbial community became more antagonistic. Overall, negative feedbacks tended to be less important early in restoration, but appeared important in remnant and older restored prairies. Our results provide evidence that it may be advantageous for management practices to take negative soil feedbacks into consideration when trying to recreate the diversity of tallgrass prairies.     

Plant and soil seed bank diversity across a range of ages of Eucalyptus grandis plantations afforested on arable lands

Aims

Plantation forests are often assumed to have reduced biodiversity relative to unmanaged forests. However, existing knowledge is based on studies of rotation-aged tree crops. We investigated how Eucalyptus afforestation of agricultural land affected plant species composition and biodiversity across a range of plantation ages (1–10 years). We also studied whether the soil seed bank could contribute to regeneration of existing vegetation in such plantations.

Methods

We used a chronosequence approach to evaluate plant and seed species composition and diversity in forests and soil seed banks. We also quantified the similarity of seed banks and aboveground vegetation within plantation sites of a given age. Plantation sites were also compared to a nearby, mature pine forest.

Results

Total plant species number, density and diversity in Eucalyptus grandis plantations increased for the first 3 years plantation establishment, then stabilized or decreased for the next 1–2 years and then increase significantly over the following years. Species number and density in soil seed bank increased significantly with plantation age only after an initial 6-year decrease. Shannon–Wiener index of total species diversity did not significantly differ with plantation age. The understory vegetation and soil seed bank were dominated by pioneer species in the first 3 years, but intermediate-successional and shade-tolerant species gradually invaded as plantations developed further. After 7 years, E. grandis plantation understories were composed of mainly shade-tolerant species. Nevertheless, the diversity of the diversity of intermediate-successional in soil seed banks were higher than that of shade-tolerant species in soil seed banks at this age range (7–10 year). Among species successfully germinated from soil seed banks, 48 % were not found in the aboveground plant community. Similarities between the species in the soil seed bank and the aboveground vegetation were low for both plantation and control forests and did not significantly change with plantation ages.

Conclusions

E. grandis likely produces a changing microclimate during plantation development, which in turn drives composition and diversity dynamics in understory vegetation and soil seed banks after the afforestation of agricultural land. The first 4 years after plantation establishment is associated with lower plant and soil seed bank diversity, meriting a greater focus on biodiversity stabilization and possibly longer rotation periods.     

Wild bee community change over a 26‐year chronosequence of restored tallgrass prairie

Restoration efforts often focus on plants, but additionally require the establishment and long‐term persistence of diverse groups of nontarget organisms, such as bees, for important ecosystem functions and meeting restoration goals. We investigated long‐term patterns in the response of bees to habitat restoration by sampling bee communities along a 26‐year chronosequence of restored tallgrass prairie in north‐central Illinois, U.S.A. Specifically, we examined how bee communities changed over time since restoration in terms of (1) abundance and richness, (2) community composition, and (3) the two components of beta diversity, one‐to‐one species replacement, and changes in species richness. Bee abundance and raw richness increased with restoration age from the low level of the pre‐restoration (agricultural) sites to the target level of the remnant prairie within the first 2–3 years after restoration, and these high levels were maintained throughout the entire restoration chronosequence. Bee community composition of the youngest restored sites differed from that of prairie remnants, but 5–7 years post‐restoration the community composition of restored prairie converged with that of remnants. Landscape context, particularly nearby wooded land, was found to affect abundance, rarefied richness, and community composition. Partitioning overall beta diversity between sites into species replacement and richness effects revealed that the main driver of community change over time was the gradual accumulation of species, rather than one‐to‐one species replacement. At the spatial and temporal scales we studied, we conclude that prairie restoration efforts targeting plants also successfully restore bee communities.     

Covariance between disturbance and soil resources dictates the invasibility of northern fescue prairies

Increasing environmental impacts of exotic organisms have refocused attention on the ability of diverse communities to resist biological invaders. Although resource availability, often related to natural and anthropogenic disturbances, appears central to the invasibility of biological communities, understanding the links between resources, diversity and invasibility is often confounded by the covariance among key variables. To test the hypothesis that community invasibility remains contingent on the type and intensity of disturbance and their impacts on plant community diversity and resource availability, we designed an experiment testing the invasibility of northern fescue prairies by smooth brome (Bromus inermis Leyss.), a Eurasian perennial grass, threatening the structure and function of prairie remnants throughout the Great Plains. Using soil disturbances and herbicide, we imposed treatments manipulating the diversity and resource availability of native prairies. Our observations demonstrate that the vulnerability of native prairies to exotic plant invasions remains contingent on resources. While the establishment of smooth brome seedlings increased with increasing disturbance, its impact depended on the availability of soil nitrogen. As a result, soil burial treatments, simulating disturbance by northern pocket gophers, provided poor recruitment areas for smooth brome, and their low levels of soil moisture and nitrogen, combined with the rapid recovery of the prairie community, compromised seedling establishment. Emphasizing the covariance of diversity and key environmental variables following disturbance, our findings illustrate the importance of disturbance type and intensity on community invasibility. Such a consideration is critical in the conservation and restoration of native prairie remnants throughout the Great Plains.     

Structural Attributes of Schizachyrium scoparium in Restored Texas Blackland Prairies

Structural attributes of the C₄, perennial bunchgrass Schizachyrium scoparium in restored prairies may be affected by the time since restoration. One hundred plants each in 8‐, 17‐, and 23‐year‐old restored prairies and a native Texas Blackland prairie were assessed for the presence/absence and diameter of a hollow crown (i.e., dead center portion), degree of fragmentation, plant height, and tiller density. Structural attributes of S. scoparium plants were generally (1) different between recent (8 years) and older (17 and 23 years) restored prairies (2) similar between the 17‐ and 23‐year‐old restored prairies, and (3) more similar between the 8‐ and 17‐year restored prairies and the native, remnant prairie than between the 23‐year restored prairie and the native prairie. Plants were shorter in restored prairies, regardless of time since restoration, than in the native prairie. Mean basal area of plants was 80–163% greater in the 17‐ and 23‐year restored prairies compared with the native and 8‐year restored prairies. Percentage of hollow crowns and fragmentation was smallest in the 8‐year restored prairie, largest in the 17‐ and 23‐year restored prairies, and intermediate in the native prairie. Tiller density exhibited inverse second‐order polynomial decreases with increasing plant basal area for all prairies. In contrast to tiller density, diameter of hollow crowns increased logarithmically with increasing plant basal area. Structural attributes of S. scoparium in restored prairies changed predictably with age, despite growing in different communities.     

Mycorrhizal suppression alters plant productivity and forb establishment in a grass-dominated prairie restoration

A fundamental goal of restoration is the re-establishment of plant diversity representative of native vegetation. However, many prairie restorations or Conservation Reserve Program sites have been seeded with warm-season grasses, leading to grass-dominated, low-diversity restorations not representative of native grasslands. These dominant grasses are strongly mycotrophic, while many subordinate forb species appear to be less dependent on mycorrhizal symbiosis. Therefore, manipulating arbuscular mycorrhizal fungi (AMF) may be useful in promoting establishment and growth of forb species in grass-dominated prairie restorations. To assess the potential role of mycorrhizae in affecting the productivity and community composition of restored tallgrass prairie, we conducted a 4-year field experiment on an 8-year-old grassland restoration at the Konza Prairie in northeastern Kansas, USA. At the initiation of our study, seeds of 12 forb species varying in degree of mycorrhizal dependence were added to established grass-dominated plots. Replicate plots were treated bi-weekly with a soil drench of fungicide (Topsin-M^®) over four growing seasons and compared to non-treated control plots to assess the role of AMF in affecting plant species composition, productivity, leaf tissue quality, and diversity in restored tallgrass prairie. Topsin applications successfully reduced mycorrhizal colonization of grass roots to approximately 60–80% relative to roots in control plots. Four years of mycorrhizal suppression reduced productivity of the dominant grasses and increased plant species richness and diversity. These results highlight the importance of mycorrhizae as mediators of plant productivity and community dynamics in restored tallgrass prairie and indicate that temporarily suppressing AMF decreases productivity of the dominant C4 grasses and allows for establishment of seeded forb species.     

Plant-Feeding Hemiptera and Orthoptera Communities in Native and Restored Mesic Tallgrass Prairies

Aboveground Hemiptera and Orthoptera communities were compared among three native and three restored mesic tallgrass prairies along the Platte River in central Nebraska to assess both the relative success of restored sites and the relationship between insect and plant communities. Hemiptera and Orthoptera were sampled using sweep nets in early June, mid-July, and mid-August 2000. Plant species composition was assessed in early June and mid-August. A total of 89 Auchenorrhyncha (71 Cicadellidae, 15 Fulgoroidea, and 3 Membracidae) and 23 orthopterans (15 Acrididae and 8 Tettigoniidae) were collected. Eighty-five plant species were observed in combined study sites. Shannon diversity was significantly higher at restored prairie for Cicadellidae ( H '= 1.38), Fulgoroidea ( H '= 0.796), and Membracidae ( H '= 0.290), which comprised the majority of individual insects collected, but significantly higher at native prairie for Acrididae ( H '= 0.560) and Tettigoniidae ( H '= 0.480) ( p ≤ 0.05). Species richness was comparable except for Acrididae which were significantly higher in restored prairie. Density of insects generally followed species diversity but was only significantly higher in restored areas for Membracidae. The number of remnant-dependent species collected was comparable for both native prairie ( n = 15) and restored prairie ( n = 15). These results suggest that, at least for Hemiptera, differences in insect communities between native and restored prairie may best be explained by the presence of insect host plants rather than by whether a site is native or restored.     

Responses of plant and bird communities to prescribed burning in tallgrass prairies

Historic losses and fragmentation of tallgrass prairie habitat to agriculture and urban development have led to declines in diversity and abundance of plants and birds associated with such habitat. Prescribed burning is a management strategy that has potential for restoring and rejuvenating prairies in fragmented landscapes, and through such restoration, might create habitat for birds dependent upon prairies. To provide improved data for management decision-making regarding the use of prescribed fire in tallgrass prairies, we compared responses of plant and bird communities on five burned and five unburned tallgrass prairie fragments at the DeSoto National Wildlife Refuge, Iowa, USA, from 1995 to 1997. Overall species richness and diversity were unaffected by burning, but individual species of plants and birds were affected by year-treatment interactions, including northern bobwhite (Colinus virginianus) and ring-necked pheasant (Phasianus colchicus), which showed time-delayed increases in density on burned sites. Analyses of species/area relationships indicated that, collectively, many small sites did make significant contributions to plant biodiversity at landscape levels, supporting the overall conservation value of prairie fragments. In contrast, most birds species were present on larger sites. Thus, higher biodiversity in bird communities which contain area-sensitive species might require larger sites able to support larger, more stable populations, greater habitat heterogeneity, and greater opportunity for niche separation.