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1.
It was found during experiments on isolated frog spinal cord involving extracellular recording from the dorsal roots (sucrose bridging) and intracellular recording from motoneurons by microelectrodes that 10 mM of the M-cholinomimetic arecoline produces motoneuronal depolarization which is matched by depolarizing electronic ventral root potentials and a rise in motoneuronal input resistance. Arecoline changes synaptic transmission by increasing the amplitude of postsynaptic potentials during intracellular recording and that of motoneuronal reflex discharges in the ventral roots but reduces the duration of dorsal root potentials. In the presence of arecoline, L-glutamate-induced motoneuronal response increases. Facilitation of synaptic transmission produced by arecoline in the spinal cord is bound up with cholinergic M2- activation, since it is suppressed by atropine but not by low concentrations of pirenzipine; it is also coupled with a reduction in adenylcyclase activity. When motoneuronal postsynaptic response has been suppressed, as in the case of surplus calcium or theophylline, arecoline produces an inhibitory effect on the amplitude of motoneuronal monosynaptic reflex discharges which is suppressed by pirenzipine at a concentration of 1×10–7 M. This would indicate the presence at the primary afferent terminals of presynaptic cholinergic M1 receptors which mediate its inhibition of impulses of transmitter release. This effect is independent of changes in cyclic nucleotide concentration.A. M. Gorkii Medical Institute, Donetsk. Translated from Neirofiziologiya, Vol. 19, No. 3, pp. 399–405, May–June, 1987. 相似文献
2.
Experiments on anesthetized and immobilized cats showed that repeated antidromic discharges can be evoked in 32.5% of sympathetic preganglionic neurons of the lateral horns in segments T3, T8–9, and L2 of the spinal cord, with intervals of 16 msec or more between them, which is much greater than the refractory period of these neurons. This feature was shown not to be connected with the properties of axons of that group of neurons and, in particular, with their after-subnormality. After orthodromic discharges in neurons of this group, for a much longer period of time than could be accounted for by possible collision, no antidromic discharges likewise were evoked. As a result of antidromic activation of some of these neurons in one segment, definite inhibition of the orthodromic response of other neurons of the same segment appeared, etiher by a reflex mechanism or through stimulation of descending pathways. The results point definitely to the existence of a mechanism of recurrent inhibition in some sympathetic preganglionic neurons of the lateral horns.I. P. Pavlov Institute of Physiology, Academy of Sciences of the USSR, Leningrad. Translated from Neirofiziologiya, Vol. 9, No. 4, pp. 382–389, July–August, 1977. 相似文献
3.
Evoked electrical discharges in the spinal cord roots and dorsal surface ipsilateral to the previously severed sciatic nerve (as well as on the contralateral side) were investigated in rats one, three, seven, and 14 days after tractotomy. Monosynaptic reflex discharges in the ventral roots were found to return to 20–40% of the level of this parameter as measured on the contralateral side within seven and 14 days after tractotomy. Mean amplitude of antidromic dorsal root discharges, afferent peak, and the N1 component of potential(s) at the dorsal surface ipsilateral to the severed nerve barely altered, remaining significantly lower than on the contralateral side. Mechanisms are suggested for the increase in monosynaptic reflex ventral root discharges ipsilateral to the severed nerve following tractotomy — thought to be largely due to raised sensitivity to transmitter at the motoneuronal membrane resulting from degeneration of synapses of descending pathways.Medical Institute of the Ukrainian Ministry of Health, Dnepropetrovsk. Translated from Neirofiziologiya, Vol. 21, No. 3, pp. 366–371, May–June, 1989. 相似文献
4.
Parameters of the reflex discharges evoked by spinal dorsal root stimulation were measured in rats with the sciatic nerve and spinal cord (at low thorasic level) transected five days earlier. Monosynaptic discharges in the ventral roots were found to increase after the operation; the degree of increase was significantly higher as compared with that observed after isolated transections of the spinal cord or the nerve. The combined lesion of the nerve and spinal cord could result in the appearance of high-amplitude reflex discharge components, probably of a polysynaptic nature. We concluded, from the comparison of modifications of reflex discharges, that the mechanisms underlying spinal hyperreflexia after nerve or spinal cord lesions differ considerably from each other.Neirofiziologiya/Neurophysiology, Vol. 26, No. 3, pp. 197–202, May–June, 1994. 相似文献
5.
The effects of dopamine on ventral root potential produced by a single supramaximal dorsal root stimulation of the dorsal root was investigated during experiments on isolated superfused spinal cord segments from 10–16-day old rats. A reciprocal dose-dependent inhibition of the mono- and polysynaptic components of reflex response was also observed. Minimum effective concentration was 1×10–8 M dopamine. Extent of reflex response increased in step with dopamine concentration, so that the amplitude of the monosynaptic component of ventral root potential was decreased by 20% and 87% of baseline level by the action of 10–4 and 10–3 M dopamine respectively on the cord. The amplitude of the polysynaptic component was thereby decreased by an average of 18% and 87%. Findings indicate that dopaminergic brainstem-spinal pathways contribute to the governing of impulse transmission in the segmental reflex arcs. Inhibition of dopaminergic synaptic transmission probably underlies the increase in latency already described in the literature, as well as the increase observed in the threshold of reflex motor response to nociceptive action following either stimulation of the dopaminergic brainstem structures or intravenous administration of dopamine agonists.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 18, No. 5, pp. 616–621, September–October, 1986. 相似文献
6.
Experiments on superfused isolated spinal cord preparations from rats aged 8–13 days showed that noradrenal in and serotonin have only a weak effect on monosynaptic reflex discharges but a substantial effect on polysynaptic motoneuronal discharges: noradrenalin potentiates whereas serotonin inhibits them. Both amines inhibit dorsal root potentials evoked by stimulation of high-threshold afferents. Potentiation of polysynaptic motoneuronal discharges induced by noradrenalin is connected with hyperpolarization of high-threshold afferents due to inhibition of the function of neurons in the substantia gelatinosa, and with increased excitability of interneurons participating in the generation of motoneuronal discharges. Serotonin inhibits polysynaptic motoneuronal discharges through its direct depolarizing effect on terminals of high-threshold afferents and depression of interneuron activity responsible for these discharges. Adrenergic and serotonin receptors, mediating these effects of noradrenalin and serotonin, were subjected to pharmacologic analysis.A. M. Gor'kii Donetsk Medical Institute. Translated from Neirofiziologiya, Vol. 14, No. 3, pp. 241–247, May–June, 1982. 相似文献
7.
The effects on dorsal root potentials of applying dopamine to the perfusing fluid were investigated in experiments on a segment isolated from the spinal cord of 13- to 18-day-old rats. Dopamine induced slow, dose-dependent depolarization in motoneurons in 28 trials out of 32, retained in the solution blocking synaptic transmission. Threshold concentration of dopamine in the normal perfusing fluid measured 1·10–6 M and 1·10–5 M in a calcium-free perfusate containing magnesium or manganese ions. Depolarization was accompanied by an increased rate of motor discharges recorded from the ventral root. Segmental reflex response produced by dorsal root stimulation was depressed following depolarization. Hyperpolarization in response to dopamine was observed in 4 out of 32 experiments. Dopamine-induced electrotonic dorsal root potentials were suppressed by prior haloperidol application to the brain.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 19, No. 6, pp. 735–741, November–December, 1987. 相似文献
8.
Experiments on anesthetized spinal cats showed that ammonium acetate, injected intravenously (2–4 mmoles/kg) inhibits the depolarization of the central endings of primary afferent fibers activated by stimulation of afferent nerves. Inhibition of primary afferent depolarization is transient in character and develops parallel with depression of postsynaptic inhibition of monosynaptic reflexes. The depression produced by the action of ammonium was not due to blocking of negative postsynaptic potentials of the dorsal surface of the spinal cord or blocking of reflex electrical discharges in the ventral spinal roots. It is suggested that depression of primary afferent depolarization is due to a decrease in the emf for synaptic ion currents producing depolarization.Allergologic Research Laboratory, Academy of Medical Sciences of the USSR, Moscow. Translated from Neirofiziologiya, Vol. 9, No. 1, pp. 52–60, January–February, 1977. 相似文献
9.
Microelectrode discharges of potentials have been realized from segmentary interneurons of the dorsal horn and intermediate nucleus of the spinal cord in cat at the L6–L7 level by electrical stimulation of the sensorimotor region of the brain cortex. It has been established that corticifugal influences on segmentary interneurons of the system of the flexor reflex and on neurons activated by high threshold muscle afferents (groups Ib, II, and III), or high threshold cutaneous afferents are predominantly excitatory. Interneurons activated by muscle afferents of group Ia or by the lowest threshold cutaneous fibers are weakly subjected to pyramidal influences. The mean latencies of excitatory postsynaptic potentials (EPSP's) and discharges evoked under the influence of pyramidal volley, for the neurons under study in the system of afferents of the flexor reflex are equal to 11.8±2.6 and 20.1±1.8 msec, respectively; for interneurons, excited only by high threshold muscle afferents, they are equal to 15.5±3.6 and 16.3±2.2 msec, respectively; and for interneurons, excited by high threshold cutaneous fibers they are equal to 11.8±2.6 and 18.3±1.4 msec, respectively. Possible pathways of activating segmentary interneurons from the lateral sensorimotor region of the brain cortex have been discussed.The A. A. Bogomolets Institute of Physiology, Academy of Sciences, Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 2, No. 1, pp. 17–25, January–February, 1970. 相似文献
10.
We investigated inhibition of the N1-component of the spinal cord dorsal potential (CDP) evoked by experimental stimulation of the n. peroneus in spinal cats. Stimulation was carried out following two conditioning stimuli administered at different time intervals to the same or different cutaneous nerves. The interval between the last conditioning stimulus and the experimental one remained constant (20 msec). It is demonstrated that there is no dependence between weakening of inhibitory action of the second conditioning stimulus and inhibition of the dorsal horn interneurons excited by it that generate the N1-component of the CDP. It is hypothesized that mechanisms which act on the principle of negative feedback are present in the vincinity of the synaptic junctions of cutaneous afferent fibers with neurons of the substantia gelationsa, and that these mechanisms restrict the development of presynaptic inhibition during inflow of a series of afferent impulses into the cord.Dnepropetrovsk State University. Translated from Neirofiziologia, Vol. 1, No. 3, pp. 253–261, November–December, 1969. 相似文献
11.
Facilitation of the second of two consecutive test EEG quanta (the summated monosynaptic potentials of the synapses of one axon arborizing in layer F of the frog tectum) was investigated in the normal and under conditions of raised extracellular Ca2+ and Mg2+ concentration. Intensification of paired-pulse facilitation (×1.4–2.4) was observed at the shortest interstimulus intervals (of 2.5–5 msec). The distribution of maximum levels for facilitation of EEG quanta was bimodal at levels 1.95 and 1.65, on the basis of which two groups were identified, one potentiating EEG quanta more than the other. The time course of paired-pulse facilitation of both groups of EEG quanta can be broken down into two exponential components with time constants of 5–6, 140–150 and 6–8, 60–70 msec respectively. Bimodal distribution of maximum paired-pulse levels in the normal, together with findings from experiments involving raised Ca2+ and Mg2+ concentrations would indicate that facilitation of frog retinotectal synapses is dependent on the quantal release of transmitter; it may thus be postulated that this release reaches near-saturation point in the normal. It is suggested that two types of axonal terminal arborizations whose synapses differ in the quantal content of transmitter release are present in layer F of the frog tectum. These axonal arbors could well originate from different class 3 and 5 retinal detectors.Z. Yanushkevichyus Institute of Physiology and Pathology of the Cardiovascular System of the Kaunas Medical Institute. Translated from Neirofiziologiya, Vol. 18, No. 1, pp. 45–55, January–February, 1986. 相似文献
12.
E. V. Maksimova 《Neurophysiology》1971,3(1):51-56
Some characteristics of spinal reflex reaction inhibition were studied in cat fetuses during the last three weeks of antenatal development. The experiments were conducted on fetuses with intact placental circulation. Restoration of the excitability of the spinal reflex arcs was very slow after stimulation of the dorsal root by a single stimulus. In embryos studied 20 days before birth the full inhibition of reflex responses lasted about 500 msec. Even 2–3 sec after a single stimulation of the afferent fibers the amplitude of the reflex response to the second stimulus was only 30–40% of the control value. It was determined that such long postactivation depression is unrelated to refractoriness or antidromic inhibition. The presence of a prolonged intense depolarization of afferent terminal fibers at these stages suggests a presynaptic inhibition as one of the most probable reasons for the prolonged postactivation depression. Another important factor in the appearance of postactivation depression is probably the morphologic and functional immaturity of synaptic structures. A reciprocal inhibition was observed in cat fetuses on the 10–12th antenatal day. On the basis of these data it is suggested that in embryogenesis presynaptic inhibition considerably precedes that of postsynaptic fibers.Institute of Higher Nervous Activity and Neurophysiology, Academy of Sciences of the USSR, Moscow. Translated from Neirofiziologiya, Vol. 3, No. 1, pp. 68–75, January–February, 1971. 相似文献
13.
In experiments on spinal cats changes in the second negative postsynaptic component (N2) of the dorsal surface potential (DSP) of the spinal cord recorded in the region of segment L7 was used as the index of inhibition of segmental dorsal horn interneurons. Conditioning and testing stimuli were applied at increasing time intervals to the popliteal and superficial peroneal nerves respectively. Changes in the N2 component were compared with changes in the N1 component of the DSP, reflecting mainly activity of nonsegmental ascending dorsal horn interneurons. After an initial short facilitation a conditioning volley of pulses evokes prolonged (over 500 msec) inhibition of the N2 component, characterized by the presence of two maxima (on the average at the 16th and 80th milliseconds) which indicate that two systems with different latent periods play a role in this inhibition. In its shape and temporal characteristics the curve of inhibition of the N2 component corresponds to the two-component dorsal root potential (DRP) recorded in spinal animals in response to stimulation of flexor afferents (FRA) [8, 19]. Together with other features, this similarity is evidence of the presynaptic nature of this inhibition. Intravenous injection of hexobarbital has a stronger action on inhibition of the N2 component, leading to a marked increase in its depth and duration. Suggestions are made regarding the functional organization of systems responsible for presynaptic inhibition of segmental dorsal horn interneurons.Deceased.Dnepropetrovsk State University. Translated from Neirofiziolgiya, Vol. 4, No. 1, pp. 75–82, January–February, 1972. 相似文献
14.
Using the method of microelectrode (intracellular and extracellular) recording, the mechanism of inhibition following reflex discharge in interneurons of the lumbosacral section of the spinal cord of cats on activation of cutaneous and high-threshold muscle afferents was studied. It was shown that the postdischarge depression of the reflex responses 10–20 msec after the moment of activation of the neuron is due to afterprocesses in the same neuron and presynaptic pathways. The depression of spike potentials from the 20th to the 100th msec is produced by inhibitory postsynaptic potentials (IPSP). During the development of IPSP the inhibition of spike potentials can be due to both a decrease of the depolarization of the postsynaptic membrane below the critical threshold and a decrease of sensitivity of the cell membrane to the depolarizing action of the excitatory postsynaptic potential (EPSP). At intervals between the stimuli of 30–100 msec the duration of EPSP after the first stimulus does not differ from that after the second stimulus. Hence, it is suggested that the presynaptic mechanisms do not play an essential part in this type of inhibition of interneurons. The inhibition following the excitation favors the formation of a discrete message to the neurons of higher orders.I. P. Pavlov Institute of Physiology, Academy of Sciences of the USSR, Leningrad. Translated from Neirofiziologiya, Vol. 2, No. 1, pp. 3–9, January–February, 1970. 相似文献
15.
Effective facilitation of the scratch reflex was observed in experiments on recently decerebrated and spinal cats in response to application of strychnine solution to the dorsal surface of the spinal cord. The receptive fields of the scratch reflex in this case depended on the segments to which the strychnine was applied. The receptive fields of a scratch reflex evoked by D-tubocurarine coincided with those of the intact adult animals, but the receptive fields for strychnine application were wider and corresponded to those of the scratch reflex in kittens [1, 10]. It is suggested that strychnine application abolishes the inhibition of the afferent inputs of the scratch reflex in the lower cervical segments of the spinal cord which has developed during ontogeny.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 10, No. 6, pp. 622–625, November–December, 1978. 相似文献
16.
Effects of dopamine on dorsal root potentials were investigated during experiments on a segment of spinal cord isolated from 12- to 18-day-old rats. Applying dopamine to the brain was found to produce a slow, reversible, dose-dependent depolarization at primary afferent fiber terminals. This dopamine-induced depolarization was retained during complete blockade of synaptic transmission brought about by exchanging calcium ions in the perfusing fluid by magnesium or manganese ions. Minimum dopamine concentration required to produce this effect was 1·10–10–1·10–9 M. Peak amplitude of depolarization equaled 1.5 mV. Duration of this reaction ranged from 5.5 to 36.7 min, depending on the duration and concentration of dopamine application. Depolarizing response to dopamine differed considerably from GABA-induced dorsal root depolarization in amplitude and rate of rise. Haloperidol, a dopamine antagonist, reduced dopamine-induced dorsal root depolarization. Findings indicate that dopamine acts directly on the membrane of primary afferent fiber terminals, shifting membrane potential toward depolarization. This raises the possibility that dopaminergic brainstem-spinal pathways may exert an effect on sensory information transmission in segmental reflex arcs already traveling to the spinal cord.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 19, No. 6, pp. 741–748, November–December, 1987. 相似文献
17.
Changes in the N1-component and P-phase of the dorsal surface potential (DSP) of the spinal cord evoked by test stimulation of the posterior tibial nerve after conditioning stimulation of the sural nerve were investigated in anesthetized cats. The test responses were inhibited if stimulation was applied at short intervals. They then recovered to some extent, but after 1.8–2.2 msec, a further prolonged period of inhibition began. The initial inhibition was connected with occlusion of synaptic action, and the subsequent prolonged inhibition with the development of presynaptic inhibition. The latent periods of prolonged inhibition of the N1-component and P-phase of the DSP (2 msec) were almost exactly identical, and the curves showing the diminution of the initial occlusion of these components were very similar. The results demonstrate that presynaptic inhibition of the interneurons generating the N1-component of the DSP and of cells of the substantia gelatinosa which participate in depolarization of the presynaptic terminals of the cutaneous afferents is due to the action of depolarizing systems with similar temporal characteristics.Dnepropetrovsk State University. Translated from Neirofiziologiya, Vol. 4, No. 5, pp. 510–515, September–October, 1972. 相似文献
18.
Microinjections of aspartic acid and chlorpromazine into the region of the locus coeruleus, which strengthen spontaneous unit activity in that structure, in decerebellate cats anesthetized with chloralose, led to depression of the inhibitory influence of flexor reflex afferents on extensor discharges, but did not change the facilitatory action of these afferents on flexor monosynaptic discharges and had no effect on recurrent inhibition of extensor discharges or reduced it. Microinjection of noradrenalin into this region, which depresses spontaneous unit activity in the locus coeruleus, or of procaine, which blocks action potential generation in neurons, led to potentiation of the inhibitory action of flexor reflex afferents on extensor discharges and to strengthening of recurrent inhibition, but did not affect the facilitatory action of these afferents on flexor discharges. The role of tonic descending influences of the locus coeruleus in the control of spinal inhibition evoked by flexor reflex afferents is discussed.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 13, No. 3, pp. 247–256, May–June, 1981. 相似文献
19.
Reflex discharges in intercostal nerves and activity of reticulospinal fibers of the ventral and lateral funiculi, evoked by stimulation of the reticular formation and of the splanchnic and intercostal nerves were investigated in cats anesthetized with chloralose (50 mg/kg). Brain-stem neuronal structures participating in the "relaying" of spino-bulbo-spinal activity were shown to lie both in the medial zones of the medullary and pontine reticular formation and in its more lateral regions; they include reticulospinal neurons and also neurons with no projection into the spinal cord. Structures whose stimulation led to prolonged (300–800 msec) inhibition of reflex spino-bulbo-spinal activity were widely represented in the brain stem, especially in the pons. Analogous inhibition of this activity was observed during conditioning stimulation of the nerves. Reticulospinal fibers of the ventral (conduction velocity 16–120 m/sec) and lateral (17–100 m/sec) funiculi were shown to be able to participate in the conduction of spino-bulbo-spinal activity to spinal neurons. In the first case fibers with conduction velocities of 40–120 m/sec were evidently most effective. Evidence was obtained that prolonged inhibition of this activity can take place at the supraspinal level.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Institute of Normal and Pathological Physiology, Slovak Academy of Sciences, Bratislava, Czechoslovakia. Translated from Neirofiziologiya, Vol. 8, No. 4, pp. 373–383, July–August, 1976. 相似文献
20.
The effects of stimulation of the dorsal funiculus on dorsal surface potentials (DSPs) of the spinal cord evoked by stimulation of a peripheral nerve and on antidromic action potentials (AAPs) evoked by stimulation of terminal branches of primary afferent fibers and recorded from the afferent nerve or dorsal root, were investigated in acute experiments on spinal cats and on cats anesthetized with pentobarbital and chloralose. Stimulation of the dorsal funiculus led to biphasic inhibition of the N1-component of the DSP with maxima at the 15th–30th and 60th–80th milliseconds between the conditioning and testing stimuli. Maximal reinforcement of the AAP was found with these intervals. Bilateral division of the dorsal funiculi between the point of application of the conditioning stimuli and the point of recording the DSP abolished the first wave of inhibition of the DSP and the reinforcement of the AAP. After total transection of the cord above the site of conditioning stimulation the picture was unchanged. It is concluded that the initial changes in DSP and AAP are due to activation of the presynaptic inhibition mechanism by antidromic impulses traveling along nerve fibers running in the dorsal funiculus. Repeated inhibition of the DSP, like reinforcement of the AAP, can possibly be attributed to activation of similar inhibitory mechanisms through the propriospinal neurons of the spinal cord.Dnepropetrovsk State University. Translated from Neirofiziologiya, Vol. 5, No. 4, pp. 401–405, July–August, 1973. 相似文献