姜寨遗址先民食谱分析

尽管通过C、N稳定同位素分析已对陕西临潼姜寨遗址先民的食物结构和粟作农业进行了初步探索,但研究中的一些重要问题,如不同时期先民食物结构的演变,姜寨与半坡、史家先民食物结构间的差异等,依然未能深入讨论。为此,本文对姜寨遗址出土的不同时期(一期、二期)人骨进行了C、N稳定同位素分析,试图揭示先民食物结构的演变历程,探索姜寨与半坡、史家先民食物结构间的差异,并初步探讨产生这种差异的可能原因。人骨的δ13C平均值(-9.7±1.0‰)和δ15 N平均值(8.5±0.5‰),表明粟类食物(包括粟类作物以及依赖于粟类作物的动物等)在姜寨先民食谱中占据主要地位,且动物资源在食物中的比例相对较低。两期先民的δ13C和δ15N值,无显著性差异,表明先民一直从事粟作农业以及家畜的饲养活动。食谱分析并未发现第二期先民食用更多野生动物的证据,这可能与先民样品量相对偏少、二期先民食用的野生动物比例上升较小、动物类食物在先民食谱中所占比例较小等原因有关。对比地理位置毗邻、文化年代相近的姜寨、史家、半坡遗址先民δ13C值,姜寨(-9.7±1.0‰,N=19)与史家(-10.0±0.7‰,N=9)接近,而远高于半坡(-14.8±1.9‰,N=5),表明半坡遗址粟作农业的种植规模要逊于姜寨和史家遗址。我们认为,不同遗址间先民δ13C值的差异,可能受半坡样品量偏少、遗址间小生态环境不同等因素的影响。     

哈民忙哈遗址人和动物骨骼的C、N稳定同位素分析

哈民忙哈遗址是迄今为止内蒙古乃至东北地区发现面积最大的一处史前聚落遗址,为重建当时的生产和生活情况、文化习俗甚至思想观念等都提供了弥足珍贵的实物资料。其中,重建该遗址先民的食物结构和生业经济,有助于揭示哈民忙哈遗址产生和兴盛的动因及科尔沁沙地史前文化的变迁过程。目前,多学科的研究成果已经初步显示哈民忙哈遗址先民的生业经济具有多样性,但各种生业模式的比重以及是否存在家畜饲养等问题还缺乏相应的了解。本文对该遗址87例人骨与18例动物骨骼进行了C、N稳定同位素分析,还原了人和动物的食物结构及生业经济面貌。结果显示,陆生野生动物的食物结构主要基于C₃植物类食物和少量的C₄农作物,而犬科动物则体现出家养动物的食谱特性：食物主要依赖C₄食物和人类食物残羹。C₄类粟黍农作物和以此饲喂的动物是该遗址先民的主要食物来源,其中粟黍农作物在食物结构中的地位尤其重要。在该遗址中,女性摄入相对较多的粟黍和相对较少的肉类,与男性在食物结构上存在显著差异。农耕、家畜饲养是先民最重要的经济行为,狩猎、渔猎和采集是生业的重要补充。     

海洋性聚落先民的食物结构:昙石山遗址新石器时代晚期人骨的碳氮稳定同位素分析

本文对18例昙石山文化墓葬出土人骨进行骨胶原的提取和碳氮稳定同位素测定,并通过污染鉴别,其中的17例为有效数据。有效骨样的δ~(13)C值在-20.9‰~-16.5‰范围,δ~(15)N值在8.5‰~14.4‰范围。就稳定同位素整体分析结果而言,昙石山先民主体所处营养级较高,以广泛的肉食来源和一定量的C_3类植物为生。推测肉食来源中主要有大量的海生类资源,也包含了陆生动物。进一步的分析表明,昙石山先民的食物营养级可能与社会贫富并无关联。单因素方差分析显示,未成年人的食物结构和成人基本相似,但可能更偏向于食用高营养级的食物;两性的食性未表现显著性的差异。作为一个海洋性聚落,昙石山遗址的经济活动应以渔猎采集为主,水稻种植很可能仅为辅助性的经济行为。     

海洋性聚落先民的食物结构:昙石山遗址新石器时代晚期人骨的碳氮稳定同位素分析北大核心CSCDCSSCI

本文对18例昙石山文化墓葬出土人骨进行骨胶原的提取和碳氮稳定同位素测定,并通过污染鉴别,其中的17例为有效数据。有效骨样的δ^(13)C值在-20.9‰^-16.5‰范围,δ^(15)N值在8.5‰~14.4‰范围。就稳定同位素整体分析结果而言,昙石山先民主体所处营养级较高,以广泛的肉食来源和一定量的C_3类植物为生。推测肉食来源中主要有大量的海生类资源,也包含了陆生动物。进一步的分析表明,昙石山先民的食物营养级可能与社会贫富并无关联。单因素方差分析显示,未成年人的食物结构和成人基本相似,但可能更偏向于食用高营养级的食物;两性的食性未表现显著性的差异。作为一个海洋性聚落,昙石山遗址的经济活动应以渔猎采集为主,水稻种植很可能仅为辅助性的经济行为。     

新疆巴里坤东黑沟遗址出土人骨的碳氮同位素分析

本文以新疆巴里坤东黑沟遗址出土的人骨为研究对象, 测定骨中的碳、氮同位素比值, 揭示该地区古代先民的食谱特征, 探索当时人们的生活与生产方式、经济形态等历史信息。研究结果发现: 人骨样品的δ13C值分布在-18.96‰~-17.74‰范围内, 样品的δ15N值均较高, 分布在12.64‰~14.33‰范围内, 表明新疆巴里坤东黑沟遗址先民的食物结构是肉类食物在日常饮食生活占有相当大的比例。根据考古发掘出土的动植物遗存, 肉类的获取应来源于驯养的牛、羊等或部分狩猎所得的鹿等动物, 植物性食物主要来源于大麦、小麦等C3类作物, 这与其生活的环境和以游牧为主, 农业和狩猎为辅的经济形态密切相关; 男性与女性的食物结构相同。     

内蒙古察右前旗庙子沟遗址新石器时代人骨的稳定同位素分析

稳定同位素分析技术近年来发展为复原古代民族食物结构、社会经济模式的有效手段。本文在应用该技术对庙子沟新石器时代遗址出土人骨中的C、N同位素比值进行了测定。结果显示,庙子沟新石器时代居民日常饮食习惯中保持着相当比例的动物性食物摄入,植物类食物的摄入中以C4类植物为主。结合其他相关资料,我们认为该组居民是以农业生产方式为主,狩猎业、采集业和渔猎业在经济生活中占据重要地位。本文的研究结果可以为复原新石器时代北方农牧交错带居民的经济模式研究提供有益的线索。     

城子崖遗址植硅体反映的生业经济模式

龙山文化是中国史前社会形态演进的关键阶段,亦是农业强化生产的关键时期。城子崖遗址是鲁北平原史前区域中心城址,其生业经济研究有助于理解该地区社会复杂化及文明进程。本文对城子崖遗址龙山时期不同遗迹单位的15份土样进行了系统的植硅体分析,尝试探讨了该遗址龙山文化时期的社会发展和生业经济水平、农作物生产和加工方式、野生植物资源利用情况以及各遗迹堆积及其所反映的人类行为活动信息。结果显示,该遗址龙山文化时期已形成粟、黍、稻、小麦、稗(可能)的农作物组合方式;同时,广泛采集利用聚落周边的自然植物资源,是农业与采集业并存发展的生业经济模式。先民在作物栽培中进行了锄草、灌溉等较为精细的管理,其中,黍较粟更具耐旱抗病特性,加上田间管理需求较低而被优先选择栽培。先民在收获作物时,采用类似割穗、掐穗等方法以减少作物茎秆及杂草混入,随后在户外进行小规模地脱壳、扬场工作。此外,根据灰坑中植硅体的组合特征可将其分为生活垃圾、谷物加工、蓄水淘米三个类型,水井和墓葬内的植硅体则分别与生活环境和丧葬环节等信息相关。     

通过食谱分析探讨田螺山与梁王城遗址先民对渔业资源的利用

对渔业食用资源的利用是人类生业经济的重要方面,然而至今尚无专文介绍如何定量分析渔业食物资源在先民食物结构中所占的比例。本文采用利用同位素传递信号重建食谱(FRUITS)模型,以田螺山遗址与梁王城遗址已发表的先民和动植物稳定同位素数据为例,对先民食物结构中的多种食物资源比重进行分析。结果显示,梁王城遗址渔业资源在食谱中占5%~22%;田螺山遗址淡水渔业资源在食谱中占5%~20%,而海洋渔业资源在10%以下。     

山西乡宁内阳垣遗址先民食物结构分析

本文应用C、N稳定同位素分析方法,对山西乡宁内阳垣遗址先民的食物结构进行了分析,旨在揭示该遗址先民的生活方式及社会经济状况,探索戎狄对该遗址先民的影响。分析表明,该遗址先民的δ13C平均值为-8.27‰,δ15N平均值为9.54‰,由此推测他们主要的经济模式应为畜牧业。考古学证据显示该遗址主体文化为晋,但食物结构分析却反映,先民的经济模式主要为畜牧业,明显受到了游牧民族(戎狄)的影响。样品XNM50具有异常的δ13C值(-15.36‰)和较高的δ15N值(9.88‰),当与其从事狩猎活动有关。δ13C和δ15N的统计分析表明,不同性别的先民在食物结构上无明显差异,而不同规格的墓葬却差异明显。     

广东湛江鲤鱼墩遗址人骨的C、N稳定同位素分析:华南新石器时代先民生活方式初探

相对于长江流域和黄河流域而言,华南地区先民生活方式的了解一直较为缺乏。广东湛江鲤鱼墩遗址人骨的C和N稳定同位素分析,显示此遗址的先民主要以海生类资源为食,陆生资源(包括可能的块茎类原始农业和动物)只占次要地位。通过与约同时代黄河流域、长江流域先民的稳定同位素数据比较,可以看出,早在6000年前,我国先民的生活方式就已出现3大类型。     

Evaluating the utility of stable isotope analyses of archived freshwater sample materials

We evaluated the potential utility of stable isotope analysis of tissues commonly archived by aquatic biologists. Previous studies with chemically preserved samples have shown contradictory results, which present an obstacle for the use of archived sample materials. We tested the effects of ethanol and formalin preservation on zooplankton and of ethanol on benthic macroinvertebrate δ¹³C and δ¹⁵N values. We found that neither formalin nor ethanol had a significant effect on δ¹³C and δ¹⁵N values of preserved zooplankton. Nor did ethanol significantly affect δ¹³C or δ¹⁵N values of macroinvertebrates. However, ethanol preservation slightly, but significantly decreased C:N ratios of both zooplankton and macroinvertebrates, probably reflecting some extraction of lipids. Overall, the effects of preservatives on δ¹³C and δ¹⁵N values that we observed were minor. We also compared δ¹³C and δ¹⁵N values analysed from roach scales and perch operculum bones with those analysed from muscle tissue. Decalcification of scales and operculum bones only slightly improved our comparison to muscle tissue δ¹³C and δ¹⁵N values. Decalcified scales had slightly higher δ¹³C and lower δ¹⁵N values. Similarly, decalcified operculum bones showed slightly increased δ¹³C and decreased δ¹⁵N values to those for fish muscle. Our results confirm that scales and operculum bones can provide a suitable proxy for fish muscle in isotope studies with minor correction. We conclude that various archived sample materials can indeed be used with confidence for historical reconstructions of freshwater food webs by stable isotope analysis. Handling editor: K. Martens     

青海喇家遗址人类遗骸的锶同位素比值分析

喇家遗址位于青海省民和县,主体为齐家文化的遗存,距今大约3900~4300年。喇家遗址古代人类迁移活动的研究,对于探索黄河上游古代文明,推动该地区齐家文化研究,有着积极的学术意义。本文首先采用电感耦合等离子体质谱技术对喇家遗址出土的22个个体的44份牙釉质和骨骼样品进行了元素分析,根据检测结果排除了受到污染的2份骨骼样品。其次,通过热电离质谱分析方法对喇家遗址出土22个个体的42份牙釉质和骨骼样品及8个猪牙釉质样品的锶同位素比值进行了测定。检测数据表明,猪牙釉质的锶同位素比值的标准偏差远小于其他动物,可以推断猪由当地饲养的可能性是最大的,因此其牙釉质锶同位素比值可以代表当地的锶同位素水平。经过计算得到8个猪牙釉质的锶同位素比值的平均值为0.710930,将该平均值加或减2倍标准偏差可以得到喇家遗址当地的锶同位素比值范围在0.711058~0.710802之间。以此为前提可以推测,本次检测的22个个体中,有17个个体牙釉质的锶同位素比值在遗址当地的锶同位素比值范围内,可能为本地出生。还有2个个体牙釉质在当地的锶同位素比值范围上下限附近,姑且存疑;仅有3个个体牙釉质的锶同位素比值在遗址当地的锶同位素比值范围以外,表明这些个体可能在其他地区出生,去世后埋葬在遗址中。此外,数据分析还显示,本地出生人群牙釉质的锶同位素比值平均值与骨骼的锶同位素比值平均值比较接近,暗示喇家遗址当地古人类生活方式可能比较统一。     

Analysis of mitochondrial metabolism in situ: Combining stable isotope labeling with selective permeabilization

To date, it is well-established that mitochondrial dysfunction does not only play a vital role in cancer but also in other pathological conditions such as neurodegenerative diseases and inflammation. An important tool for the analysis of cellular metabolism is the application of stable isotope labeled substrates, which allow for the tracing of atoms throughout metabolic networks. While such analyses yield very detailed information about intracellular fluxes, the determination of compartment specific fluxes is far more challenging. Most approaches for the deconvolution of compartmented metabolism use computational models whereas experimental methods are rare. Here, we developed an experimental setup based on selective permeabilization of the cytosolic membrane that allows for the administration of stable isotope labeled substrates directly to mitochondria. We demonstrate how this approach can be used to infer metabolic changes in mitochondria induced by either chemical or genetic perturbations and give an outlook on its potential applications.     

Seasonal changes in the diets of migrant and non-migrant nectarivorous bats as revealed by carbon stable isotope analysis

Three species of nectar-feeding bats migrate from tropical and subtropical Mexico into the Sonoran and Chihuahuan deserts during the spring and summer months. We examined geographic and seasonal changes in the diet of one migrant species, Leptonycteris curasoae, using carbon stable isotope techniques to determine the relative importance of C3 and CAM (Cactaceae, Agavaceae) plants in its diet. We also examined the diet of a non-migratory nectar-feeding bat, Glossophaga soricina, from southern Mexico using the same techniques. We found that L. curasoae feeds extensively or exclusively on CAM plants during migration and in the northern part of its range and feeds mostly on C3 plants in southern Mexico. This bat is a year-round resident on Baja California where it is a CAM specialist. The non-migrant G. soricina feeds mostly on C3 plants year-round. Phenological data suggest that certain species of columnar cacti and at least one group of paniculate Agaves on the Mexican mainland provide a spatio-temporally predictable nectar corridor along which nectarivorous bats may migrate in the spring and fall, respectively. Different flowering schedules of Agaves in Baja California appear to promote year-round dietary specialization and perhaps non-migratory behavior in nectar-feeding bats living there.     

Stable nitrogen and carbon isotope ratios in bone collagen as indices of prehistoric dietary dependence on marine and terrestrial resources in southern California

The ratios of 15N to 14N and 13C to 12C tend to be higher in marine than in terrestrial organisms. The concentrations of these isotopes in human bone collagen consequently can be used to make inferences about the contribution of marine and terrestrial resources to prehistoric diets. The utility of studying 15N/14N and 13C/12C ratios in conjunction with each other is illustrated by our analysis of 40 human burials from archaeological sites in the Santa Barbara Channel area of southern California. The mean delta 13C and delta 15N values (in per mil) of collagen from these skeletons decrease progressively from the Channel Islands (delta 13C = -14.0, delta 15N = +16.3) to the mainland coast (delta 13C = -14.5, delta 15N = +14.9) to the interior (delta 13C = -17.2, delta 15N = +10.9). These data suggest that Indians living on the Channel Islands during the late prehistoric period were heavily dependent on marine resources. The inhabitants of the mainland interior, in contrast, had a diet composed largely of terrestrial foods. From their isotope ratios, it appears that the Indians who lived on the mainland coast consumed a mixed diet containing substantial quantities of both marine and terrestrial resources. Differences in 15N/14N and 13C/12C ratios of individuals from mainland sites dating from the early and late prehistoric periods show that the marine component of the diet increased substantially through time. These isotopic data are consistent with pathological, faunal, and artifactual evidence of increased marine resource exploitation during the late prehistoric period.     

云南省耿马佛洞地遗址发掘简报

佛洞地遗址位于云南省临沧市耿马傣族佤族自治县勐简乡勐简村大军赛村民小组燕子洞,坐落于一处东南开口的二叠纪灰岩穿洞,南临南汀河。2016～2017年,临沧市文物管理所在公路考古调勘期间发现该遗址;为进一步认识滇西地区旧石器时代晚期文化,2017～2018年对该遗址开展考古发掘工作。发掘区域位于洞内第四台面到第五台面间,共发掘20 m²,出土了包括石制品、动植物化石等在内的大量遗物。初步地层年代学分析显示,遗址时代为距今18400～14000年,共包含3期连续文化,文化遗物以石制品为主,总数达到9735件。佛洞地遗址作为一处热带-亚热带生境下的史前遗址,为我们构建旧石器时代晚期滇西地区文化序列、探讨特定自然生态背景下史前人类的文化适应提供了重要参考。     

Measurement of salicylic acid in human serum using stable isotope dilution and gas chromatography-mass spectrometry

A simple, highly selective, and sensitive method using stable isotope dilution and gas chromatography-mass spectrometry has been developed to quantify salicylic acid (SA) at concentrations naturally occurring in biological fluids, such as in the serum of subjects not taking aspirin. After extraction of liquid-liquid with diethyl ether and ethyl acetate and preparation of the tert-butyldimethylsilyl derivative, SA content was detected using deuterated SA as internal standard. The mean recovery of SA from serum was 85 +/- 6%. Intra- and interday precision and % relative error were <15% in all cases.With a detection limit of 0.6 ng and a quantification limit of 2 ng, the method is therefore also adequate for population studies because of the small amount of blood necessary to perform the analyses.     

Assessment of anadromous salmon resources in the diet of the Alexander Archipelago wolf using stable isotope analysis

The Alexander Archipelago wolf (Canis lupus ligoni) is unique to southeast Alaska, occurring on islands south of Frederick Sound and along the mainland between Dixon Entrance and Yakutat Bay. Sitka black-tailed deer (Odocoileus hemionus sitkensis) are an important prey species for wolves across the southern part of the region. Spawning salmon (Onchorynchus sp.) are seasonally available but their presence in wolf diets has not previously been quantified. We examined the range of bone collagen δ¹³C and δ¹⁵N values for wolves throughout southeast (n = 163) and interior (n = 50) Alaska and used a dual-isotope mixing model to determine the relative contribution of salmon-derived marine protein in the diet. Southeast Alaska wolves consumed significantly more salmon (mean ± SE: 18.3 ± 1.2%) than did wolves from interior Alaska (9.1 ± 0.6%, P<0.001). Wolves on the southeast Alaska mainland appeared to have higher marine isotopic signatures than island wolves, although this difference was not significant. Variation among individual wolf diets was higher for southeast than for interior Alaska wolves, and variation was highest in coastal mainland wolf diets (P<0.001). Marine resources may augment the diet of southeast Alaska wolves during seasonal or annual fluctuations in the availability of deer, particularly in those areas on the mainland where densities of terrestrial ungulates are relatively low. Received: 1 July 1998 / Accepted: 23 February 1999     

Trophic structure of common marine species in the Bohai Strait,North China Sea,based on carbon and nitrogen stable isotope ratios

Trophic relationships among common coastal species in the Bohai Strait, North China Sea, were investigated in this study using stable isotope ratios of carbon (¹³C/¹²C) and nitrogen (¹⁵N/¹⁴N) as tracers. During the research cruises of this study, 16 species of invertebrates and 18 species of fishes were collected, as were various food sources (phytoplankton, macroalgae, and sediment). The carbon and nitrogen isotope ratios of all collected samples were measured and used for trophic level analyses. The results showed that the grazers Oregonia gracilis, Notoacmea schrenckii, Chlorostoma rustica, and Chelon affinis, preferentially consumed Zostera marina (p < 0.01), whereas Anthocidaris crassispina preferred Ulva conglobata (p < 0.01). Two trophic models based on nitrogen isotope ratios were built to identify the trophic level of each species. The model that combined food sources was more appropriate than the model that used a single primary producer to identify the relative trophic positions of primary consumers. Scombermorus niphonius, an important fishery resource, was at the top trophic level. The seastar Asterias amurensis was at the highest level among the invertebrates and directly threatened production of shellfish. Based on the trophic level and food source relationships identified in this study, we gave some advice for ecological restoration, such as optimizing the structure of food source distribution, limiting seastar numbers, and improving the applicability of habitat for fishery species.