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1.
The activities of nodular uricase and allantoinase, the compositionof bleeding sap N, estimated rates of xylary N translocationand ureide assimilation in shoot tissues were compared in acold-tolerant (194) and sensitive (Seafarer, SF) line of nodulatedPhaseolus vulgaris grown under three different temperature regimes.Uricase activity increased with increasing growth temperatureand was generally greater in nodules of 194 than in SF at anyparticularly temperature. Extractable allantoinase activity,on the other hand, was highest in nodules from plants grownat the coolest temperature (15/10 C day/night) and there waslittle or no difference in activity between the lines. There was little difference in sap composition between linesor amongst temperature treatments. Ureides contributed between8091 percent of the total sap N in both lines grown at25/15 or 20/15 C with a slightly lower per cent (6584)when grown at 15/10 C. Estimated rates of N translocation werehigher in 194 than SF at the coolest growth temperature. Increasesin rates of N translocation between successive harvests of eitherline were often correlated with increases in total N accumulationand also an accumulation of ureides in stems plus petioles butnot leaves. Generally leaves assimilated all of the ureidesinto other compounds at all growth temperatures. Nodules andshoots of either line did not accumulate ureides at 15/10 C.194 Accumulated greater amounts of ureides in stems and petiolesthan SF when grown at the two warmer temperatures. The resultsare discussed in relation to the ability of 194 to fix greateramounts of N than SF at suboptimal growth temperatures. Phaseolus vulgaris, common bean, uricase, allantoinase, sap composition, ureide assimilation, low temperature stress 相似文献
2.
In nodulated common bean (Phaseolus vulgaris L.), there is typicallya period of N stress between 15 and 20 d after emergence (DAE),due to a lack of synchronization between the depletion of Nin the cotyledons and the beginning of N2 fixation and transport.Screening trials identified some Rhizobium leguminosarum bv.phaseoli strains with which symptoms of N deficiency were notvisible (precocious strains). Cultivar Negro Argelwas then inoculated with two traditional strains(C-05 and CIAT 727) and two precocious strains(CNPAF 146 and CNPAF 512), and plants were harvested from 8to 30 DAE. There were no differences between the two groupsof strains in nodule dry weight or in the acetylene reductionrates between 8 and 16 DAE. However, nodules induced by theprecocious strains showed earlier onset of glutaminesynthetase (GS) (EC 6.3.1.2
[EC]
) and glutamate synthase (GOGAT)(EC 1.4.1.14
[EC]
) activities, and ureide synthesis. The N concentrationin the nodules formed by precocious strains variedfrom 4.2 to 4.5%, whereas with the traditionalstrains, it increased from 3.2% at 8 DAE to 65% at 18 DAE, atwhich time plants exhibited N-deficiency symptoms. By 21 DAE,GS and GOGAT activities in traditional noduleswere increased, as well as the ureide-N-concentration in thexylem sap, nodule N content declined to 4.5% and the leavesbecame green. These results suggest that the N stress with traditionalstrains is not a limitation in early N2 fixation activity butrather in the rates of expression of the processes of N assimilationand transport. Key words: Glutamate synthase, glutamine synthetase, nitrogen fixation, Phaseolus vulgaris, Rhizobium 相似文献
3.
Primary leaves of 2-week-old bush bean plants (Phaseolus vulgariscv Top Crop) were treated with one of 36 combinationsof cyclohexanecarboxylic acid (CHCA) and 2,4-dichlorophenoxyacetic acid (2,4-D), each at six equally spaced concentrationsFive, 7, 9 and 11 days after treatment, one plant from eachcombination was harvested, and primary leaves were monitoredfor nitrate reductase activity (NRA), nitrate nitrogen (N) concentration,organic N concentration, and d wt NRA was assayed by an in vivoprocedure Data from the resulting 6 x 6 x 4 factorial configuration foreach of the four responses were analysed using the method oforthogonal polynomials, which is described in detail This method,based on equally spaced levels, produced independent polynomialexpressions in CHCA concentration, 2,4-D concentration, and/ortime-to-harvest after treatment, which were then fitted to thedata on each response by simple linear regression analyses.A graphical procedure was used to compare predicted responsesfrom the best fit equations with the actual data. Examination of computer-generated plots of the three-dimensionalresponse surfaces revealed that NRA, organic N concentration,and d wt decreased with increasing 2,4-D concentration, whereasnitrate N content increased Increasing CHCA concentration generallyhad the opposite effect on NRA and nitrate N content, althoughinteraction between the two chemicals was evident in the latterresponse Organic N content remained unchanged as CHCA concentrationincreased, while d wt first increased then decreased Possibleexplanations for these results are discussed, as are the advantagesof using factorial designs with equally-spaced levels in plantgrowth studies. Phaseolus vulgaris L, bush bean, nitrogen metabolism, plant growth regulators, cyclohexanecarboxylic acid, 2,4-dichlorophenoxy acetic acid, regression analysis, orthogonal polynomials, response surfaces analysis 相似文献
4.
Leaf discs cut from primary leaves of Phaseolus vulgaris L cvMasterpiece were incubated at temperatures higher than the growthtemperature of 25 °C Both basal and wound ethylene productionincreased up to temperatures of 3537 5 °C, thereafterdeclining rapidly There was no detectable ethylene productionat temperatures above 42 5 °C Exposure of leaf discs tohigh temperature for 60 mm resulted in a large production ofwound ethylene when they were returned to 25 °C The magnitudeof ethylene production was related to the initial incubationtemperature as was the length of the lag period before maximumproduction was achieved The results are discussed in relationto the requirement for continued membrane integrity for ethyleneproduction ethylene, temperature, membrane permeability, Phaseolus vulgaris L, dwarf bean 相似文献
5.
Eight day old greenhouse grown Phaseolus vulgaris L. seedlingswere exposed to three different temperature regimes. The concentrationof the total free nucleotides, nucleoside triphosphates, RNAand protein was much more pronounced in seedlings exposed tothe interactive day/night temperature of 28 ?C (soil) and 15?C (air) of regime I. Seedlings treated to a low soil/air, day/nighttemperature of 15 ?C (regime II) had the lowest content of totalfree nucleotides, nucleoside triphosphates, RNA and protein.The nucleoside triphosphate content and energy charge valueof seedlings treated to a high soil/air, day/night temperatureof 28 ?C (regime III) declined much earlier than in the seedlingsexposed to temperature regimes I and II. The fresh and dry weightof seedlings exposed to temperature regime I was significantlyhigher than those seedlings exposed to the other two temperatureregimes. The slowest growth was observed in seedlings exposedto temperature regime II. The noticeably higher concentrationof ATP, pyridine nucleotides and root nodulation in seedlingsexposed to temperature regime I suggests that higher soil andlow air temperature enhances nitrogen metabolism in P. vulgarisseedlings. 相似文献
6.
CHEN FENG-LING; BENNETT MALCOLM J; CULLIMORE JULIE V. 《Journal of experimental botany》1990,41(10):1215-1221
This paper has investigated the regulation of the activitiesof glutamine synthetase (GS) and NADH-dependent glutamate synthase(NADH-GOGAT) of Phaseolus vulgaris in relation to the nitrogensupply. The activity of NADH-GOGAT II, which is the most abundantisoenzyme of glutamate synthase in root nodules of P. vulgaris,was either absent or barely detectable in other organs of thisspecies. Moreover, its activity in roots could not be inducedby ammonium. In nodules NADH-GOGAT II activity was detectedin nodules grown under an atmosphere of 80% argon: 20% oxygenand in nodules formed with a Fix- Rhizobium mutant. However,in these non-fixing nodules the activity of this isoenzyme attainedless than 15% of the activity in fixing nodules and switchingargon/oxygen grown nodules to nitrogen/oxygen led to an increasein this isoenzyme within 24 h. This effect could not be mimickedby the addition of exogenous ammonium. Ammonium addition, however,promoted nodule senescence and also led to a decrease in theactivities of nitrogenase and plant GS. In particular, the nodule-enhancedGS isoenzyme but not the GSß isoenzyme was affectedby these changes and in a manner similar to the changes in NADH-GOGATII. The activity of the NADH-GOGAT I isoenzyme was detectablein other organs of P. vulgaris and in nodules its activity alsoshowed some changes in response to the rate of dinitrogen fixation. Key words: Glutamate synthase, glutamine synthetase, nitrogen fixation, nodule metabolism, Phaseolus vulgaris 相似文献
7.
RAMOS M. L. G.; GORDON A. J.; MINCHIN F. R.; SPRENT J. I.; PARSONS R. 《Annals of botany》1999,83(1):57-63
Plants of EMGOPA-201, a drought tolerant cultivar of commonbean(Phaseolus vulgaris), were maintained either at 90% soilfield capacity (SFC) or stressed by reducing SFC to 70, 50 or30% over a 10 d period. Plant dry weight was not affected byany of these treatments although the number and weight of noduleswas reduced at 50 and 30% SFC. Nitrogenase activity, determinedby the acetylene reduction assay (ARA), was also reduced, ona plant basis, at 50% SFC and was almost stopped at 30% SFC.The latter treatment caused a marked increase in nodule O2diffusionresistance and induced nodule senescence. A time-course analysisof the 10 d 30% SFC treatment showed a decrease in leaf waterpotential from -0.5 to -0.87 MPa by 8 d, with a cessation ofdry weight increase after 3 d, when leaf water potential was-0.65 MPa. Proteins in the host plant fraction of nodules decreasedto 50% of control values by 10 d and leghaemoglobin (Lb) contentwas also lower at this stage. The activity of sucrose synthase(SS) showed a 76% reduction between 3 and 6 d, whilst glutamatesynthase (GOGAT) activity showed a 40% reduction. The activityof other key enzymes of carbon metabolism was also reduced after10 d. Nodule sucrose content increased to double that of controlnodules by 6 d, before declining back to control levels at 10d. Starch content fell by 3 d and continued to fall throughoutthe stress period. The results are discussed in terms of droughttolerance strategies in relation to growth and metabolism inwhole plants and nodules.Copyright 1999 Annals of Botany Company. Phaseolus vulgaris,common bean, water stress, nitrogen fixation, oxygen diffusion, acetylene reduction, enzyme activity, carbon metabolism. 相似文献
8.
Guye, M. G, Vigh, L. and Wilson, J. M. 1987. Recovery afterchilling: an assessment of chill-tolerance in Phaseolus spp.J.exp. BoL, 38: 691701. The chill-sensitivity of three Phaseolus spp. (eight cultivars)was assessed by measuring five different physiological parameters(leaf pigment loss, leaf diffusion resistance, relative growthrate recovery, change in leaf water content and the severityof leaf necrosis) on return to the warmth (23 ?C/18 ?C) followinga brief but severe chilling treatment (24 h at 5 ?C). In thisway the genotypes could be ranked in order of increasing chill-sensitivityas follows: P. coccineus cvs Prizewinner and Streamline, P.vulgaris cv. 251 < P. vulgaris cvs 194, 222 and Seafarer< P. vulgaris cv. Tendergreen < P. aweus cv. Berken. Key words: Chill-tolerance ranking, chlorophyll, leaf diffusion resistance, leaf water content, growth rate, carotenoid, Phaseolus 相似文献
9.
Accumulation of dry weight and leaf plus stem area were measuredin Echinochloa utilis and E. frumentacea grown at temperatureregimes from 15/10°C to 33/28°C (day/night). Tilleringand height were recorded in addition to leaf number which wassubsequently used as a developmental index. In both species shoot dry weight increased with temperatureup to 33/28°C; the increase in relative growth rate (RGR)was negligible above 27/22°C. Below 27/22°C the RGRof E. frumentacea decreased sharply and at 15/10°C it madeno effective growth. At low temperatures the RGR of E. frumentaceawas lower than that of E. utilis due to slow leaf area expansion,and in particular smaller individual leaves. E. frumentaceatillered more than E. utilis. Plant development was retardedat low temperatures but was not as responsive to temperatureas dry weight and leaf area. The different responses to temperatureof the two species were described in equations suitable forinclusion in predictive growth models. Echinochloa spp., millet, growth, development, temperature, relative growth rate 相似文献
10.
When leaf discs cut from primary leaves of Phaseolus vulgarisL. cv. Masterpiece plants grown at 25°C were incubated attemperatures below 25 °C, basal and wound ethylene productioncontinued at reduced rates. In both cases detectable levelsof ethylene were produced at 25 °C. When the rates of ethyleneproduction were plotted according to the Arrhenius equationa marked discontinuity was found at 11.4 °C which is consistentwith a membrane phase-transition at the critical chilling temperatureof the plant. Activation energies for the rate-limiting enzymereaction in ethylene production above and below the criticaltemperature have been calculated and the data interpreted asindicating the involvement of membrane-bound enzyme systemsin the biosynthesis of basal and wound ethylene. ethylene, temperature, Arrhenius plot, activation energy, Phaseolus vulgaris L., bean 相似文献
11.
WHITE P. J.; COOPER H. D.; CLARKSON D. T.; EARNSHAW M. J.; LOUGHMAN B. C. 《Annals of botany》1991,68(1):23-31
Rye (Secale cereale cv. Rheidol) and wheat (Triticum aestivumcv. Mardler) were grown at shoot/root temperatures of 20/20°C (warm grown, WG plants), 8/8 °C (cold grown, CG plants)and 20/8 °C (differential grown, DG plants). Plants fromcontrasting growth temperature regimes were standardized andcompared using a developmental timescale based on accumulatedthermal time (°C d) at the shoot meristem. Accumulationof dry matter, nitrogen and potassium were exponential overthe time period studied (150550 °C d). In rye, therates of plant dry matter and f. wt accumulation were linearlyrelated to the temperature of the shoot meristem. However, inwheat, although the rates of plant dry matter and f. wt accumulationwere temperature dependent, the linear relationship with shootmeristem temperature was weaker than in rye. The shoot/rootratio of rye was stable irrespective of growth temperature treatment,but the shoot/root ratio of wheat varied with growth temperaturetreatment. The shoot/root ratio of DG wheat was 50% greaterthan WG wheat. In both cereals, nutrient concentrations anddry matter content tended to be greater in organs exposed directlyto low temperatures. The mean specific absorption rates of nutrientswere calculated for the whole period studied for each species/temperaturecombination and were positively correlated with both plant shoot/rootratio and relative growth rate. The data suggest that nutrientuptake rates were influenced primarily by plant demand, withno indication of specific nutrient limitations at low temperatures. Nutrient accumulation, relative growth rate (RGR), rye, Secale cereale cv. Rheidol, temperature, thermal time, Triticum aestivum cv. Mardler, wheat 相似文献
12.
The effects of temperature on the growth parameters Net AssimilationRate (E), Relative Leaf Growth Rate (RLGR), Relative GrowthRate (RGR), Specific Leaf Area (SLA), and Leaf Weight Ratio(LWR) in Phaseolus vulgaris L. beans were examined. Rapid changes in SLA invalidate the conventional two-harvestmethod of estimating RGR, and make comparisons between varietiesdifficult The changes in SLA are seen to be due to independent effectsof temperature on RLGR and E, LWR being unaffected. A simple model is described which allows the time-course ofSLA and RGR to be followed by iterative integration on a computer. The results of the simulation suggest that the observed effectsof temperature on RLGR and E are sufficient to predict the changesin SLA and RGR. This should permit more useful comparisons ofgrowth parameters of varieties grown in differing conditionsto be made. 相似文献
13.
The Effects of Root Cooling and the Light--Dark Transition on Growth of Primary Leaves of Phaseolus vulgaris L. 总被引:1,自引:0,他引:1
SATTIN M.; SERAPHIN E. STACCIARINI; DALE J. E. 《Journal of experimental botany》1990,41(10):1319-1324
Use of Rotary Variable Displacement Transducers attached togrowing primary leaves of Phaseolus vulgaris has shown thatwhen the root systems were rapidly cooled from 23°C to 10°Cleaf extension rate fell to very low values within a few minutes.When the root systems were returned to 23°C leaf extensionincreased almost immediately to overshoot the control rateswithin 510 min, before declining to control values overthe next 5060 min. When lights went off at the end ofthe day cycle there was an immediate and very large increasein leaf extension rate in both root-cooled and control plants;the rate then slowly declined over the next 60 min. This effectwas seen when the photoperiod was artificially shortened orlengthened and was reduced in magnitude when the photoperiodwas ended gradually by dimming the lights. The effect was notattributable to effects on leaf temperature but appears to bethe result of an endogenous rhythm interacting with the endof the photoperiod. At the beginning of the photoperiod therewas a gradual reduction in leaf extension rate occurring over3045 min. Key words: Phaseolus vulgaris, leaf growth, extension rate, root cooling, wall extensibility, turgor 相似文献
14.
The growth responses of Actinidia chinensis raised from cuttingswere compared in 8 h short days (SD) and 16 h long days (LD)at 15, 20 and 25 °C, as well as under varying day and nighttemperatures The data obtained reveal effects on stem elongation,apparent plastochrons, leaf area and shape, as well as dry matteraccumulation and water contents of different plant parts Theseinvestigations were supplemented by studies on the effects ofapplied GA3 and ethephon Alternating day/night temperatures(thermoperiodicity) increased leaf area and d wt accumulationin LD Effects on sugar and starch contents, are described anddiscussed Unexpected effects such as very high petiole watercontents and their continuous growth, increased twisting ofthe climbing stem in SD and other findings are also reportedand discussed Actinidia chinensis, Kiwi fruit, gibberellic acid, ethephon, temperature, photoperiod, themoperiodicity 相似文献
15.
Growth performances of Crotalaria juncea L. and C. sericea Retz.have been compared at two controlled temperatures, 1620°C, and 2832 °C, with respect to increase ind. wt and leaf area, relative growth rate, leaf area ratio,specific leaf area, leaf weight ratio, net assimilation rate,the ratio of mean relative growth rate to mean relative rateof leaf area increase () and shoot/root ratios. Both the speciesgrew better at the higher temperature; however the relativegrowth rate was more affected by temperature in C. sericea thanin C. juncea. Further, it was observed to be more dependenton net assimilation rate than on the leaf area ratio. Crotalaria juncea L., Crotalaria sericea Retz., relative growth rate, leaf area ratio, specific leaf area, leaf weight ratio, leaf area increase, net assimilation rate, shoot/root ratio 相似文献
16.
Two Phaseolus vulgaris L. cultivars were grown at 20/15, 25/20,and 30/25 °C day/night temperatures in growth chambers witha 16 h thermoperiod corresponding to the photoperiod. When thefirst trifoliolate leaf was fully expanded rates of CO2 exchange(CER) were measured at 27 °C and saturating light usinginfrared gas analysis. Stomatal (rs) and mesophyll resistances,CO2 compensation points, activities of the enzymes ribulosebisphosphate carboxylase (RuBPCase), glycolate oxidase (GAO),malate dehydrogenase (MDH), and fructose-1, 6 diphosphate (FDP),chlorophyll content, Hill activities, and leaf anatomy at boththe light and electron microscope level were also investigatedin these leaves. Rates of CO2 exchange in the light, transpiration rate, andchlorophyll content increased with increasing growth temperaturewhile leaf thickness, specific leaf weight, RuBPCase activity,compensation point, and stomatal resistance decreased. Mesophyllresistance also decreased when calculated assuming zero chloroplastCO2 concentration (rm, o), but not when calculated assuminga chloroplast CO2 concentration equal to the CO2 compensationconcentration (rm, g). Average leaf size was maximal in 25/20°C plants while dark respiration, MDH activity, stomataldensity, and starch were minimal. The activities of GAO andFDP and Hill activity were not affected by temperature pretreatment. 相似文献
17.
Pansies (Viola xwittrockiana Gams.) cv. Universal Violet weresown on five dates between Jul. and Dec. 1992 and placed insix temperature-controlled glasshouse compartments set to providemean temperatures between 6.5 and 30 °C. Shoot dry weightand leaf number were recorded. A model was constructed, to analysethe effects of light and temperature on dry matter accumulation,which assumed that relative growth rate (RGR) declined linearlywith thermal time accumulated from sowing, reflecting ontogeneticdrift. Furthermore, it assumed that RGR was a semi-ellipsoidfunction of temperature, rising to an optimum of 25.3 °Cand declining thereafter, and a positive linear function oflight integral. When fitted to data collected in this studythe model accounted for 94% of the variance in RGR. Independentvalidation using data from four further crops grown in glasshousecompartments at four different set point temperatures showedthat the model could also be used to predict plant dry weightaccurately (r 2=0.98). The rate of mainstem leaf productionwas also linearly related to both light integral and temperature. Pansy; Viola xwittrockiana ; temperature; light integral; dry weight; relative growth rate; leaf number 相似文献
18.
Bean (Phaseolus vulgaris L.) plants were grown, one to fiveplants per pot, with a non-limiting supply of mineral nutrients.As plant density increased, seed and N yield per plant decreased,but those per pot remained fairly constant. The shoot: rootratio and the contribution of roots to total plant respirationwere also almost constant with changing density; the Q10 forshoot respiration was higher at maximal densities. However,growth respiratory C losses per plant over the growth periodon a seed dry matter of N yield basis were not dependent onplant densities tested. Phaseolus vulgaris L., yield, respiration, plant density 相似文献
19.
Sand-culture experiments were carried out in full daylight atsuccessive weekly intervals between March and December 1969,to investigate the effects of seasonal changes in climatic factorson the growth of Helianthus annuus and Phaseolus vulgaris inFreetown. Values for a number of growth parameters were calculatedfrom the dry weights of the leaves, stems, and roots, and fromthe leaf areas. Simultaneously the diurnal changes in climaticfactors were recorded. Multiple regressions linking light, temperature, and relativehumidity with some of the growth parameters were calculated.The total variance accounted for in the regressions of relativegrowth-rate, net assimilation rate, and leaf weight ratio onlight, temperature, and relative humidity ranged from 51 to60 per cent in P. vulgaris. In H. annuus relative humidity wasless important; the percentage proportion of total varianceaccounted for in the regression of leaf weight ratio (and leafarea ratio in both species) on light and temperature was notsignificant. The results showed that H. annuus grew faster than P. vulgaris,but the latter's growth was depressed less by the dull weatherof the rainy season. The relative growth-rates of both specieswere positively dependent on light and temperature while theirnet assimilation rates were negatively dependent on relativehumidity, and their leaf area ratios negatively dependent onlight. All parameters used except leaf area ratio and leaf areato leaf weight ratio showed seasonal variations correspondingto dry and rainy seasons. The initial and final values of leafarea ratio and weight ratios were always different but did notvary in the same direction in both species. The results are discussed in relation to similar work done elsewhere. 相似文献
20.
A commercially available bacterial fermentor was modified toallow the continuous cultivation of glucose-limited bean cells(Phaseolus vulgaris L.) at a defined pH. A steady state wasreached using a dilution rate of 0?004 h1 and was maintainedfor 4 months; during this period the dry weight and packed cellvolume of the culture remained constant; the yield for glucosewas 0?34 (dry wt./dry wt) and for carbon 0?27 (g atom/g atom).Glucose-limited cells had lower C/N ratios both for cytoplasmand cell walls than cells in the exponential phase of growthin batch cultures. 相似文献