Influence of chosen elements on the dynamics of the cariogenic process

This prospective study comprised 140 natural crowns of the teeth extracted from 31 boys and 35 men, as well as 39 girls and 35 women. They were divided into two groups. Group I consisted of primary teeth and group II consisted of permanent teeth. In each group, two subgroups were distinguished: subgroup A containing teeth without caries and subgroup B comprising carietic teeth. Zinc, iron, copper, nickel, chromium, cobalt, lead, cadmium, selenium, and strontium were determined in the samples by using the total reflection X-ray fluorescence method. Significantly higher concentrations of zinc, iron, copper, nickel, selenium, and strontium were detected in the crowns of healthy primary and permanent teeth than in the crowns of the carietic primary and permanent teeth. The concentrations of chromium, cobalt, lead, and cadmium were significantly higher in primary and permanent teeth with caries than in the healthy ones. Judging from the obtained results, we think that lower concentrations of zinc, iron, copper, nickel, selenium, and strontium together with higher concentrations of chromium, cobalt, lead, and cadmium in the carietic primary and permanent teeth, in relation with the respective concentrations of those elements in healthy teeth, can be one of the caries risk factors.     

Spatial distribution of lead in the roots of human primary teeth

Lead remains one of the most hazardous metals in our environment. The concentrations of lead in coronal dentine and enamel have previously been reported but limited information is available regarding lead levels in radicular dentine and cementum. This study reports the distribution of lead in 26 roots of 16 human maxillary primary teeth from seven individuals. In addition, calcium and phosphorous concentrations were also measured to detect any variations in the degree of mineralization in different regions of the roots. The mean lead concentration in these roots was 1.67 +/- 1.43 microg/g, which is comparable to other studies. In all cases there were higher lead concentrations in the apices of non-resorbed roots of primary maxillary teeth relative to middle and cervical regions. The findings reported here are of potential significance during the process of physiological root resorption whence periapical tissue may be exposed to higher levels of lead.     

Interaction of mineral elements in sea water and shell of oysters (Crassostrea virginica (Gmelin)) cultured in controlled and natural systems

The interaction is reported of selected chemical elements (cadmium, calcium, copper, iron, magnesium, manganese, strontium, and zinc) in cultured sea water, with soft tissues, prismatic calcite of the right valve, and foliated calcite of right and left valves of genetically similar American oysters, Crassostrea virginica (Gmelin) grown in a natural habitat and in two environmentally controlled experimental systems (flow-through and recycle). The addition of trace elements as algal nutrients in ambient sea water was reflected in higher concentrations of trace metals in shells and soft tissues of oysters grown in experimental systems. Calcium was relatively uniformly distributed in major regions of valves from the three habitats, even though its concentration fluctuated widely in sea water in experimental systems. Magnesium and strontium were most concentrated in valves of oysters grown in the recycle system (magnesium in the prismatic layer of the shell and strontium in the foliated calcite). Iron was uniformly distributed. Cadmium, copper, manganese, and zinc were most concentrated in the prismatic calcite of valves from the flow-through system. In soft tissues, calcium was more concentrated in oysters from experimental systems than in those from the natural habitat. Manganese was about equally distributed in soft tissues from the three habitats, whereas copper and iron were more concentrated in soft tissues in experimental systems than in the natural habitat, and were many times more concentrated in soft tissues than in valves from all three habitats. As concentrations of magnesium, strontium, mangenese, zinc, and cadmium increased in valves in experimental systems, pigmentation of valves decreased. The study confirmed the capacity of oysters to concentrate several elements in their valves as concentration of these elements increased in ambient sea water and disclosed the heterogeneous distribution of these elements in major regions of the valves.     

Lower strontium in two different body matrices in neurodevelopmental disorders: A preliminary report

BackgroundEnvironmental factors, including elemental homeostasis, have not been studied sufficiently in neurodevelopmental disorders (NDD). This study aims to compare the status of 13 elements in blood and deciduous teeth dentine of children having an autism spectrum disorder or attention deficit hyperactivity disorder with typically developing controls.MethodsElements including calcium, phosphorus, magnesium, iron, zinc, copper, chromium, manganese, mercury, lead, cadmium, molybdenum, and strontium in both deciduous teeth and blood were analyzed by inductively coupled plasma mass spectrometry.ResultsStrontium levels in both blood and teeth samples were found to be significantly lower in the NDD group. Additionally, blood cadmium and mercury levels, and copper/zinc ratio were higher in the NDD group.ConclusionsOur results warrant further investigation in a large series of NDD examining symptom levels and genetic variations associated with elemental homeostasis.     

Levels of Heavy Metals and Trace Elements in Umbilical Cord Blood and the Risk of Adverse Pregnancy Outcomes: a Population-Based Study

To better understand the relationship between prenatal exposure to heavy metals and trace elements and the risk of adverse pregnancy outcomes, we investigated the status of heavy metals and trace elements level in a Chinese population by collecting umbilical cord blood. Umbilical cord blood heavy metals and trace elements concentrations were determined by inductively coupled plasma–mass spectrometry. No differences with statistical significance in the median arsenic (As), cadmium (Cd), cobalt (Co), chromium (Cr), copper (Cu), manganese (Mn), nickel (Ni), lead (Pb), strontium (Sr), thallium (Tl), vanadium (V), and zinc (Zn) concentrations were observed between the adverse pregnancy outcome group and the reference group. Titanium (Ti) and antimony (Sb) were found at higher levels with statistical significance in the cord blood samples with adverse pregnancy group when compared to the ones in the reference group. The association between Ti levels and the risk of adverse pregnancy outcomes remained significant after adjusting for potential confounding factors, including newborn weight. These results indicated that environmental exposure to Ti may increase the risk of adverse pregnancy outcomes in Chinese women without occupational exposure.     

Changes in trace element concentrations in hair of growing children

Head hair concentrations of zinc, copper, manganese, and iron from a total of 418 subjects (154 male and 264 female) aged between 6 mo and 20 yr were measured mainly with flameless atomic absorption spectrophotometry. Only zinc analysis of a part of the female samples (n=140) were analyzed with inductively coupled plasma-atomic emission spectrometry. The two analytical methods showed close agreement. The mean concentration of copper and manganese were significantly higher in male subjects than in female subjects. The trace element concentrations in hair varied with the subject’s age. Zinc concentration in hair decreased from 6 mo to 14 yr in the male subjects and decreased from 6 mo to 12 yr in the female subjects. Then, the concentrations increased gradually to 20 yr in the both sexes. Age-dependent variations of copper and manganese concentrations in hair showed similar trends to those of zinc. The results of this study suggest that a higher concentration in the diet of these trace elements may be required for growing children, especially in the period of adolescence.     

Analysis of Trace and Major Elements in Bronchoalveolar Lavage Fluid of Mycoplasma Bronchopneumonia in Calves

The aim of this study was to evaluate the reliability and effectiveness of direct determination of trace and major element concentrations in bronchoalveolar lavage fluid samples from Holstein calves with Mycoplasma bronchopneumonia (n = 21) and healthy controls (n = 20). The samples were obtained during bronchoscopy using a standard examination method. A total of 18 elements (aluminum, bromine, calcium, chlorine, chromium, copper, iron, potassium, magnesium, manganese, molybdenum, nickel, phosphorous, sulfur, silicon, strontium, titanium, and zinc) were detected by particle-induced X-ray emission. The average bromine, iron, potassium, magnesium, and phosphorous concentrations were higher in calves with bronchopneumonia than in controls (p < 0.05). They were found to have higher amounts of calcium and zinc, and a higher zinc–copper ratio than that in healthy calves (p < 0.001). Based on the receiver operating characteristics curves, we propose a diagnostic cutoff point for zinc–copper ratio for identification of Mycoplasma pneumonia of 8.676. Our results indicate that assessment of the elemental composition of broncholaveolar lavage fluid is a promising diagnostic tool for Mycoplasma bronchopneumonia.     

Macro and micro-elements concentrations in Calligonum comosum wild grazing plant through its growth period

In this study, the change in the content of the macro and micro elements in the growing wild grazing plant of Calligonum comosum was tracked at the Research and Training Station of King Faisal University in Al-Hassa Governorate, Kingdom of Saudi Arabia. Mineral elements were estimated in aerial parts (plant as a whole, leaves and stem) from January-April 2020. The results showed that the concentration of nitrogen, phosphorus and potassium in the plant as a whole plant > leaves > roots, while the concentrations of calcium, magnesium, manganese, zinc and copper elements in the leaves was higher than other parts whereas the concentrations of these elements of whole plant were higher than the concentrations in roots. The results showed that the plant contents of nitrogen, potassium and zinc were the highest in March, while the concentrations of phosphorus, calcium, iron and copper were in February. The concentrations of magnesium, manganese and copper was the highest in January and April respectively. The values ​​of nitrogen, phosphorous, potassium, calcium, magnesium, iron, manganese, zinc and copper ranged from 11.1 to 18.4 g kg⁻¹, 4.17–2.33 g kg⁻¹, 13.73–18.97 g kg⁻¹, 24.50–28.90 g kg⁻¹, 10.40–12.30 gkg⁻¹, 1500–1677 mg kg⁻¹, 45.45–49.29 mg kg⁻¹, 70.70–177.23 mg kg⁻¹, 16.78–73.46 mg kg⁻¹, respectively. Furthermore, the results exhibited that the lowest values of the elements appeared in the plant roots in April. As well as, the distribution of the elements followed the normal life curve from January to April. Besides that, the evaluated elements satisfy the needs of the grazing animals' life in which this type of plant grows.     

Element profiles in blood and teeth samples of children with congenital heart diseases in comparison with healthy ones

BackgroundSome elements were claimed to play a role in the pathogenesis of congenital heart defects (CHD) and influence the general well-being and health of these children.ObjectivesWe aimed to assess the levels of some elements simultaneously in the blood and teeth samples of children with cyanotic and acyanotic CHD compared with healthy children.MethodsA total of 39 children with CHD (11 with cyanotic and 28 with acyanotic CHD) and 42 age- and sex-adjusted controls were enrolled. Levels of 13 elements, including magnesium, phosphorus, calcium, chromium, manganese, iron, copper, zinc, strontium, cadmium, lead, mercury, and molybdenum, were assessed using inductively coupled plasma mass spectrometry.ResultsChildren with cyanotic and acyanotic CHD had significantly lower teeth calcium and calcium/phosphorus ratio as compared to the controls after adjusting for confounders. The mean blood iron level was found to be significantly higher in the cyanotic CHD group compared to the other groups. In addition, children with acyanotic CHD had significantly higher teeth copper levels, higher blood molybdenum and lower blood magnesium levels compared to the healthy control group. Blood cadmium and mercury levels were found to be significantly elevated in both the cyanotic and acyanotic CHD groups compared to the healthy control group. There were no differences in toxic metal levels of teeth in cases with CHD.ConclusionMonitoring adequate and balanced gestational micronutrient intake might support not only maternal health but also fetal cardiac development and infant well-being. Supplementation of magnesium should be evaluated in patients having CHD.     

Comparisons of nine heavy metals in salt gland and liver of greater scaup (Aythya marila), black duck (Anas rubripes) and mallard (A. platyrhynchos)

Levels of nine heavy metals were measured in the livers and salt glands of greater scaup (Aythya marila), black duck (Anas rubripes) and mallard (A. platyrhynchos) from Raritan Bay, New Jersey to determine if the functioning avian salt gland concentrates heavy metals. Heavy metals examined were cadmium, cobalt, chromium, copper, lead, mercury, manganese, nickel and zinc. Heavy metal levels varied significantly by species and tissue for chromium, copper, lead, and manganese, and by tissue for cobalt, mercury, nickel and zinc. In comparing tissues cobalt was higher in the salt glands than in livers of all three species; chromium and nickel were higher in the salt gland than liver for mallard and black duck; and lead, manganese and zinc were higher in the liver than the salt gland in greater scaup. Generally metal levels were higher in the salt gland for mallard and black duck, and in the liver for greater scaup.     

Effects of Schistosoma mansoni infection on inorganic elements in the snail Biomphalaria glabrata

Inductively coupled plasma atomic emission spectrometry (ICP-AES) was used to study element ions in whole bodies of uninfected Biomphalaria glabrata snails and those experimentally infected with larval Schistosoma mansoni trematodes. Infected snails were analysed 8 weeks post-infection. Cohort snails that were left uninfected were analysed at the same time as the infected snails. Sixteen elements (aluminum, boron, barium, calcium, cadmium, copper, iron, potassium, magnesium, manganese, sodium, nickel, lead, selenium, tin and zinc) were found to be present in infected and uninfected whole bodies at concentrations above the detection limit of the ICP-AES analysis. Of these, calcium, cadmium, manganese and sodium were present in significantly higher amounts (Student's t-test, P<0.05) in whole infected versus whole uninfected snails. Variations in the present results compared with other studies reflect intrinsic differences in the larval trematode-snail systems used.     

Determination of some cationic species in temporary teeth.

Some cationic species (Ca, Mg, Sr, Cu, Zn and Pb) have been determined in temporary teeth (n = 67) sampled from a children population living in Mérida, Venezuela, with a mean age of 87.76 +/- 34.50 months within a range from 29 to 151 months. Each powdered tooth sample was analyzed for calcium, magnesium, strontium and zinc and for copper and lead by atomic absorption spectrometry with flame and electrothermal atomization, respectively. Relations between variables (elements concentration, age, sex, tooth type and caries status) were established using the Pearson correlation matrix and analysis of variance. The mean global concentrations were of 39.25 +/- 5.36; 1.08 +/- 0.81; 89.27 +/- 13.26 and 2.85 +/- 1.14 micrograms/g for Sr, Cu, Zn and Pb and of 208.45 +/- 33.18 and 8.51 +/- 2.05 mg/g for Ca and Mg, respectively. There were no changes in the teeth mineral content with respect to the children age and sex. With respect to the tooth type, there were significant differences only for strontium which varied in the order molars > canines > incisors. Although healthy teeth had higher mineral concentrations than carious teeth, there were not significant differences, proving that both, carious and healthy teeth could be used as indicators of mineral uptake. Magnesium and copper are the elements least related with the others. Significant positive correlations were found between Sr-Zn, Sr-Cu, Sr-Pb, and the only negative correlations were between Ca-Zn and Ca-Pb for the different types of teeth. While the positive association might indicate competition for anionic groups or similar affinity for the same sites in the crystalline structure, the negative correlations showed possible substitutions among the elements.     

Measurement of zinc, copper, manganese, and iron concentrations in hair of pituitary dwarfism patients using flameless atomic absorption spectrophotometry

Pituitary dwarfism (hGHD) is known to be associated with trace element deficiency, which causes improper functioning of the involved endocrine system. Previously, we reported on the head hair concentrations of zinc, copper, manganese, and iron from a total of 418 normal subjects (154 male and 264 female). In this report, we analyzed the head hair concentrations of the same four trace metals of 103 hGHD children (60 male and 43 female) under treatment with human growth hormone (hGH). These subjects ranged in age from 5 to 18 yr. The results were compared with 338 agematched normal subjects (120 male and 218 female). Both male and female hGHD showed approx 1.7 times higher zinc concentrations than normal subjects. Cheruvanky et al. reported a similar trend but with a slightly lower difference between hGHD and normal subjects. The average copper content in the hair of both male and female subjects also showed higher values for the hGHD than for the normal subjects, a trend similar to the values reported by Teraoka et al. In the case of manganese, the concentrations in hair of the hGHD were approx 50% of the values in the normal subjects. Head hair concentrations of iron in the hGHD were commensurate with the normal subjects. Because the content of trace elements in hair varies with the age of subjects, as a control, we investigated the head hair concentration of zinc from 20 healthy girls ranging in age from 10 to 18 yr. The average zinc concentration decreased from 10 to 12 yr, but no clear relation to age was observed from 13 yr and older. These trends were similar to our previous report. The zinc concentration in hair and body weight gain over a year was negatively correlated. The age variation in the content of zinc, copper, manganese, and iron in hair was measured comparing hGHD with the normal subjects in various ages. Concerning the zinc-level variation of hGHD and normal subjects, there were conspicuous differences between hGHD and normal subjects. For copper, the variations in concentration with age were similar to zinc. Regarding the age variations for manganese, hGHD had lower concentrations in hair compared to the normal subjects throughout adolescence (11–18 yr). We have studied the effects between the hair and these trace element concentrations in hGHD before and after hGH administration. These results suggest that hGH affects the metabolism of these trace elements.     

Concentrations of Trace Metals in Dominant Aquatic Plants of the Lake Provala (Vojvodina, Yugoslavia)

The trace metal (Fe, Mn, Zn, Cu, Ni, Pb, Cd, Sr, and Cr) contents in the most common submerged and floating aquatic plants Ceratophyllum demersum L., Myriophyllum spicatum L., and Nymphoides flava Hill. of Provala Lake were evaluated. Considerable higher contents of iron, manganese, zinc, nickel, lead and strontium were found in submerged species than in the floating ones. The presence of cadmium and lead in plant tissues points to a certain degree of lake water pollution.     

Gender Differences in Correlations Between the Content of Elements in Rain Water and the Frequency of Hospitalization for Arterial Hypertension, Chronic Obstructive Pulmonary Disease, and Psoriasis in Opole Voivodship, Poland, During 2000–2002

The aim of this work is to show and give a plausible explanation to gender-dependent differences in correlations between the content of selected elements in rainwater and the frequency of hospitalization by reason of arterial hypertension, chronic obstructive pulmonary disease (COPD), and psoriasis in the area of Opolskie Voivodeship, Poland, during the period 2000-2002. The elements analyzed were sodium, potassium, calcium, magnesium, manganese, iron, total phosphorus, total nitrogen, ammonium nitrogen, zinc, copper, lead, cadmium, nickel, chromium, chloride, nitrate, and sulfate. Hospitalization due to arterial hypertension was more frequent in women, whereas those for COPD and psoriasis were more frequent in men. In the case of women hospitalized for arterial hypertension, the correlations were low, except for zinc (r = 0.47) and for cadmium (r = 0.43). In men hospitalized for COPD, all of the correlation coefficients were higher than 0.4, except for phosphorus. The coefficients for nickel, ammonia nitrogen, and total nitrogen ranged from 0.5 to 0.6 and the remaining elements from 0.6 to 0.7. In women, the correlation was limited to five elements where the coefficient was r > 0.4 for chloride, calcium, nitrate, phosphorus, and chromium. In cases of hospitalization for psoriasis, the correlation in men was between 0.4 and 0.5 for chloride, phosphorus, copper, lead, and total nitrogen and greater than 5 for sulfate, potassium, calcium, iron, manganese, nitrate, and ammonium nitrogen. The correlation in women was between 0.48 and 0.5 for ammonium nitrogen and phosphorus.     

Dietary Exposure to Lead and Cadmium in Yugoslavia

Abstract

Lead exposure still represents a matter of health concern especially in Yugoslavia. To assess the exposure of normal urban population to lead and cadmium through food, a preliminary monitoring was performed on a small group of urban population. Lead, cadmium and some essential elements (calcium, zinc, iron, copper and manganese) were analysed in collected duplicate diet samples and compared to similar population in Sweden. We found that dietary exposure to lead and cadmium is similar to other countries although Yugoslav urban population is exposed to much higher concentrations of lead in air than in cities of developed countries, due to high lead in gasoline. However, daily intake of some essential elements was significantly lower.

Also populations living around lead smelters in various parts of Yugoslavia are still exposed to elevated environmental lead and cadmium levels. To assess the exposure of the population living in this area, a cumulative long-term exposure to lead was determined by measuring lead in deciduous teeth. Concentrations of lead and cadmium in vegetables, soil and meals from the same region were also analysed. Values obtained for lead and cadmium in food products grown in exposed and control area were found to be related to respective concentrations of these elements in soil as well as to the distance from the smelter. Meals prepared in this region show the same trend, revealing very high intake particularly of lead.

The influence of nutritional factors, i.e. dietary calcium on lead metabolism, was also studied. Blood lead concentration was determined in two groups of peasant women living in two regions with different dietary calcium intake. Lower blood lead values were found in the higher dietary calcium intake region.     

The effects of age and sex on seven elements of Sprague-Dawley rat organs

This study reports age-related changes in 7 element (iron, copper, zinc, manganese, mercury, cadmium and lead) concentrations in the liver, kidney and brain of male and female Sprague-Dawley rats from 1 to 364 days of age. Atomic absorption spectrometry was used for the measurements. Copper, mercury and cadmium in the male and female kidneys increased from weaning until 127 days of age, as did iron concentrations in the female liver and kidney. After 127 days, especially, the copper concentration in the female kidney and cadmium concentration in the male and female kidney increased further. Consistent and statistically significant (P less than 0.05) sex differences in element concentrations were found for three elements (iron, copper and zinc). Except for the zinc concentration in the liver from 50 to 72 days, iron (in liver and kidney), zinc (in kidney) and copper (in liver, kidney and brain) concentrations in female rats during the adult stage, were all higher than those of male rats. Isolated differences for other elements (manganese, mercury and cadmium) were also found. The data will be helpful when setting up long-term animal investigations of the biological effect of elements.     

Heavy metals in some Chinese herbal plants

The concentrations of nine heavy metals, cadmium, cobalt, copper, iron, manganese, nickel, lead, zinc and mercury in 42 Chinese herbal medicinal plants were determined. Generally, all the samples studied had, relative to the other trace metals, higher concentrations of iron, manganese, and zinc. The concentration range of the metals determined was comparable to that in many of the East Asian vegetables and fruits. A few samples were found to contain relatively higher concentrations of the toxic metals such as cadmium, lead, and mercury. This was probably caused by contamination during air-drying and preservation.     

Trace-element analysis of human teeth and bone by proton-induced X-ray emission

The objective of our study is to correlate Fe, Cu, Zn, Pb, Br, Sr, and Mo concentrations in human teeth crown, root, and bone. The samples, teeth from a lower jaw bone and the lower jaw bone itself, were obtained from a 97-yr-old female cadaver. Two hundred milligrams of crown and root of the teeth, and lower jaw bones were fixed to a Kapton film and irradiated with a 3.8-MeV external proton beam from an 8-MV FN Tandem Van de Graaff Accelerator. TheF-test was used to analyze the difference between crown, root, and bone. It resulted in the following: For Fe, Zn, and Pb, the comparison showed significant differences among the three sample populations of tooth crown, root, and bone; for Cu, Br, Sr, and Mo, the comparison showed no significant differences among all three sample populations. Several elements in the samples were highly interrelated.     

Effect of phosphorus and zinc on the growth and phosphorus,zinc, copper,iron and manganese nutrition of rice

Summary A greenhouse experiment was conducted to study the effect of phosphorus and zinc application, in three lowland alluvial rice soils (Haplustalf) on the growth of rice and the concentration of phosphorus, zinc, copper, iron and manganese in shoots and roots. The results showed that application of phosphorus and zinc significantly increased the dry matter yield of shoots, grains and roots. Application of phosphorus caused a decrease in the concentration of zinc, copper, iron and manganese both in shoots and roots. Application of zinc also similarly lowered the concentration of phosphorus, copper and iron, but increased that of manganese in shoots and roots. The decrease in the concentration of the elements in the shoots was not due to dilution effect or to the reduced rate of translocation of the elements from the roots to tops. This has been attributed more to the changes in the availability of the elements in soil resulting from the application of phosphorus and zinc.