共查询到20条相似文献,搜索用时 31 毫秒
1.
2.
3.
4.
5.
6.
7.
8.
Pituitary peptides. Resolution by gel filtration 总被引:2,自引:0,他引:2
9.
10.
Application of a thin-layer gel filtration (TLG) method using Sephadex G-200 superfine for the assay of estrogen receptor (ER) is described. The method is based on the effective separation of specific receptor protein from nonspecific binding proteins and free hormone by TLG and subsequent determination of the radioactivity bound to specific ER. By the aid of fluorescein-labeled human γ-globulin, the position of specific ER can be visualized. The assay excludes completely the undesired fluctuation depending on the concentration of coexisting proteins and permits reliable and rapid quantitation of specific ER. Association constant and concentration of specific ER can be determined with 100 mg of mammary tumor tissue. The method has the additional advantage of not requiring expensive equipment. 相似文献
11.
12.
13.
H G Pontis 《Analytical biochemistry》1968,23(2):331-333
14.
A method is described for the purification of peptides by gel filtration on Sephadex. The success of the method is due mainly to the use of 70% () pyridine-30% () 1 m aqueous ammonia, an excellent volatile solvent for peptides which does not degrade Sephadex. The method has been used to purify all of the major peptides of cowpea chlorotic mottle virus coat protein, after an initial fractionation by ion-exchange chromatography, and selected separations are used to illustrate the degree of fractionation which can be achieved. 相似文献
15.
16.
17.
Molecular weight of human placental lactogen investigated by gel filtration. 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《The Biochemical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
P Andrews 《The Biochemical journal》1969,111(5):799-800
18.
Glycopeptides obtained from rat brain by proteolytic digestion with papain have been separated from glycosaminoglycans by means of gel filtration. The glycosaminoglycans appear in the void volume, whereas the glycopeptides are retarded. Glycopeptides of groups A+B (Brunngraber et al., 1973) (MW = 3800-500) C+D (MW = 2000) which were partially resolved by the method, were identified in the elution profile. Nucleic acids, also solubilized by papain, are eluted together with the glycosaminoglycans. 相似文献
19.
20.
Peptide-protein interaction as studied by gel filtration 总被引:10,自引:0,他引:10