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1.
Introduction: Aided by the advent of advanced mass spectrometry (MS)-based technologies and methodologies, quantitative proteomics has emerged as a viable technique to capture meaningful data for candidate biomarker evaluation. To aid clinical translation, these methods generally utilize a bottom-up strategy with isotopically labeled standards and a targeted form of MS measurement. Areas covered: This article reviews the status, challenges, requirements, and potential of translating current, MS-based methods to the clinical laboratory. The described methods are discussed and contrasted within a fit-for-purpose approach, while different resources for quality control, quantitative analysis, and data interpretation are additionally provided. Expert commentary: Although great strides have been made over the past five years in developing reliable quantitative assays for plasma protein biomarkers, it is crucial for investigators to have an understanding of the clinical validation process, a major roadblock in translational research. Continued progress in method design and validation of protein assays is necessary to ultimately achieve widespread adoption and regulatory approval. 相似文献
2.
Book reviewed in this article: The World of Maluku: Eastern Indonesia in the Early Modern Period . Leonard Y. Andaya. Honolulu: University of Hawaii Press, 1993. 306 pp. Constituting the Minangkabau: Peasants, Culture, and Modernity in Colonial Indonesia . Joel S. Kahn. Providence: Berg Publishers, 1993. 314 pp. 相似文献
3.
The Yanomami and Their Interpreters: Fierce People or Fierce Interpreters? Frank A. Salamone. Lanham, MD: University Press of America, 1997. 117 pp. Spirit of the Rainforest:. Yanomamö Shaman's Story. Mark Ritchie. Chicago: Island Lake Press, 1996. 271 pp. Yanomamö Interactive: The Ax Fight. Peter Bella. Napoleon Chagnon. and Gary Seaman. Case Studies in Cultural Anthropology Multimedia Series Windows/Macintosh CDROM. Fort Worth, TX: Harcourt Brace, 1997. 相似文献
4.
The study aimed at testing chronotype and gender differences in the time of day when humans feel the greatest need for sex and the time of day they actually undertake sexual activity. A Polish sample of 565 participants aged between 18 and 57 was tested. In females, regardless of chronotype, the greatest need for sex occurred between 18:00 and 24:00, but a secondary peak appeared only in morning types at 6:00–9:00. In males, the greatest need for sex occurred either in the morning or evening hours: in evening types at 9:00–12:00 and 18:00–3:00; in neither types at 6:00–9:00 and 18:00–24:00; in morning types at 6:00–12:00 and 18:00–24:00. Considering time of day when subjects were undertaking sexual activity most frequently, this appeared between 18:00 and 24:00 for all the participants, and prolonged until 3:00 at night in evening type males. Morningness preference was more strongly related to the timing of need for sex than to the timing of actual sexual activity ( r?=??0.275 vs. r?=??0.174), while the timing of desire and the timing of sexual activity were positively, but moderately related ( r?=?0.320). 相似文献
5.
Indians, Markets, and Rainforests: Theory, Methods, and Analysis. Ricardo A. Godoy. New York: Columbia University Press, 2001. 274 pp. 相似文献
6.
Lords of the Lebanese Marclies: Violence and Narrative in an Arab Society. Michael Gilsenan. Berkeley: University of California Press, 1996. 377 pp. Memories of Revolt: The 1936-1939 Rebellion and the Palestinian National Past. Ted Swedenburg. Minneapolis: University of Minnesota Press, 1995. 259 pp. 相似文献
7.
Abstract Shoshana Zuboff. In the Age of the Smart Machine: The Future of Work and Power. New York: Basic Books, 1988, 468 pp. $11.95 paperback. George Gilder. Microcosm: The Quantum Revolution in Economics and Technology. New York: Simon &; Schuster, 1989. 426 pp. $19.95 hardcover. Loren Baritz. The Good Life. New York: Alfred A. Knopf, Inc., 1988. 361 pp. $19.95 hardcover. Miles Orville. The Real Thing: Imitation and Authority in American Culture, 1880–1940. Chapel Hill: University of North Carolina Press, 1989. 382 pp. $14.95 paperback. Peter Shaw. The War Against the Intellect. Iowa City: The University of Iowa Press, 1989. 181 pp. $10.95 paperback. Robert Alter. The Pleasures of Reading in an Ideological Age. New York: Simon &; Schuster, 1989. 250 pp. $18.95 hardcover. Ian Crofton. A Dictionary of Art Quotations. New York: Macmillan Publishing Company, 1989. $19.95 hardcover. 相似文献
9.
A stacking sodium dodecyl sulfate polyacrylamide gel electrophoresis system has been used to resolve and quantify all the major myofibrillar protein components (actin, myosin, tropomyosin, and troponin C, T, and I). Quantification was achieved by densitometry of the fast green-stained gels calibrated with the use of purified proteins. The approximate molar ratios of these proteins in rabbit muscle are: actin: myosin: tropomyosin: troponin T: troponin I: troponin C = 7:1:1:1:1:1. On the basis of these results and available structural information one obtains an estimate of 254 myosin molecules per thick filament. 相似文献
10.
Parasabella Bush, 1905 is reintroduced as a replacement name for Demonax Kinberg, 1867 (Annelida: Polychaeta: Sabellidae) which is a junior homonym of Demonax Thomson, 1860 (Insecta: Coleoptera: Cerambycidae). 相似文献
11.
Twenty two species of ectoparasites (Family Nycteribiidae: Nycteribia ( Listropoda) schmidlii schmidlii, Nycteribia ( Nycteribia) latreillii, Nycteribia ( Nycteribia) pedicularia, Penicillidia ( Penicillidia) dufourii, and Phthiridium biarticulatum; Family Streblidae: Brachytarsina ( Brachytarsina) flavipennis and Raymondia huberi; Order Siphonaptera: Rhinolophopsylla unipectinata arabs, Nycteridopsylla longiceps, Araeopsylla gestroi, Ischnopsyllus intermedius, and Ischnopsyllus octactenus; Order Heteroptera: Cimex pipistrelli, Cimex lectularius, and Cacodmus vicinus; Class Arachnida: Order Mesostigmata: Spinturnix myoti and Eyndhovenia euryalis; Order Ixodida: Family Argasidae: Argas transgariepinus and Argas vespertilionis; Family Ixodidae: Hyalomma dromedarii, Ixodes ricinus, and Ixodes vespertilionis) were recovered from 19 bat species in Algeria. New host records for bats are recorded for the first time: N. schmidlii from Rh. clivosus and R. cystops; N. latreillii from Rh. blasii and P. gaisleri; R. huberi from Rh. clivosus; C. pipistrelli from E. isabellinus and H. savii; C. vicinus from E. isabellinus; S. myoti from P. gaisleri; E. euryalis from P. gaisleri and Rh. blasii; A. vespertilionis from P. gaisleri; I. ricinus from T. teniotis and Rh. hipposideros and H. dromedarii from P. kuhlii. Raymondia huberi is recorded for the first time from Algeria. 相似文献
12.
Purpose: The evaluation of the association between different exposure to pesticides and different types of comet images among a group of 26 recruited subject. Material and methods: We have recruited 26 subjects (farmers and not farmers). They were submitted to a blood drawing of 10?mL and a questionnaire was administered to investigate personal habits and work history. By means of the comet assay it was assessed the oxidative DNA damage of peripheral lymphocytes by pesticides in the exposed and non-exposed workers. Result: The data measured by means of the comet were log transformed and the associations (odds ratios – ORs) with the single pesticides were calculated. The regression analysis shows a significant dose effects relationships between the Intensity Score of pesticides exposures tail moment (TM) and other measured parameters. Insecticides TM OR?=?17.00 [1.47?–?196.41]. This relationship is independent from the smoking habits. Conclusions: TM values higher than the 75° percentile were significantly associated with the exposure to particular insecticides such as chlorpyriphos, deltamethrin and with other pesticides (near the statistical significance). 相似文献
13.
Revisioning Women, Health, and Healing: Feminist, Cultural, and Technoscience Perspectives. Adele E. Clarke and Virginia L. Olesen. eds. New York and London: Routledge, 1999. + 374 pp. 相似文献
14.
One Discipline, Four Ways: British, German, French, and American Anthropology. Fredrik Barth, Andre Gingrich, Robert Parkin, and Sydel Silverman. Chicago: University of Chicago Press, 2005. 406 pp. 相似文献
15.
Encyclopedia of Precolonial Africa: Archaeology, History, Languages, Cultures, and Environments. Joseph O. Vogel. ed. Walnut Creek, CA: AltaMira Press, 1997.606 pp. 相似文献
16.
旱生芦苇在水分限制、元素匮乏的环境条件下,经长期进化适应形成了自身独特的生理生态特征,研究其C、N、P化学计量特征随生长季节的变化规律有助于深入了解该植物生存和适应策略。系统分析了克里雅绿洲旱生芦苇根、茎、叶的C、N、P化学计量特征及其季节动态,深入探讨了不同生长季、不同器官以及两因素的交互作用对以上特征的影响。结果表明:旱生芦苇C、N、P含量均值分别为393.36、12.43、1.25 mg/g,C∶N、N∶P、C∶P均值分别为54.55、9.96、441.27。整个生长季内芦苇各器官间C、N、P平均含量的变化规律一致,为叶茎根,C、N、P化学计量比的变化规律不一致;芦苇C含量随生长季节的变化不断增加,N、P随季节的变化逐渐减少,C、N、P化学计量比随季节的变化规律也不尽相同。对芦苇C、N、P含量及其化学计量比整体变异来源分析显示,生长季节的变化对芦苇C、P、C∶N、C∶P变化的贡献大于器官间差异,器官间差异对芦苇N、N∶P变化的贡献大于生长季节的变化;说明芦苇生长发育过程中各生长季各器官对元素的吸收利用具有特异性。结合N、P元素含量及N∶P值的大小可知,研究区芦苇生长受到N、P共同限制,且更易受N元素的限制。 相似文献
17.
Women as Subjects: South Asian Histories. Nita Kumar. ed Charlottesville: University of Virginia Press, 1994.239 pp. From the Margins of Hindu Marriage: Essays on Gender, Religion and Culture. Lindsey Harlan and Paul B. Courtright. eds. New York: Oxford University Press, 1995.250 pp. The White Woman's Other Burden: Western Women and South Asia during the British Rule. Kumari Jayawardena New York: Routledge, 1995.310 pp. 相似文献
18.
目的:探讨食管癌术后发生呼吸衰竭(RF)的原因及防治疗措施。方法:将194例行食管癌手术的患者按术后是否发生呼衰分为观察组(36例)和对照组(158例),比较分析相关因素,对发生RF的患者行气管插管呼吸机辅助和气管切开的抢救。结果:36例RF均在术后24~72h发生,33例痊愈,死亡3例。结论:年龄、肺功能、手术时间、血清白蛋白含量、术后其它并发症及是否吸烟等都是诱发RF的重要因素,术前积极治疗肺部合并症、改善肺功能、缩短手术时间、防止染是预防和减少RF发生的主要措施。 相似文献
19.
Making Majorities: Constituting the Nation in Japan, Korea, China, Malaysia, Fiji, Turkey, and the United States. Dru C. Gladney. ed. Stanford, CA: Stanford University Press, 1998. 350 pp. 相似文献
20.
Antibodies to Toxoplasma gondii were investigated in serum samples of field mice, Microtus fortis, from Yuanjiang, Hunan Province, People's Republic of China. The modified agglutination test (MAT) incorporating formalin-fixed whole tachyzoites and mercaptoethanol was used to determine antibodies. Antibodies to T. gondii (MAT > or = 1:20) were found in 36 (29%) of 124 trapped mice. The antibody titers of positive sera (percentage in parentheses) were 1:20 (8.9), 1:40 (3.2), 1:80 (3.2), 1:160 (1.6), 1:320 (1.6), 1:640 (1.6), 1:1,280 (1.6), 1: 2,560 (0.8), and > 1:2,560 (6.5). No antibody to T. gondii was found in 104 sera of laboratory-bred M. fortis infected with Schistosoma japonicum between 1 and 45 days after infection. 相似文献
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