Advances in Tools to Determine the Glycan-Binding Specificities of Lectins and Antibodies

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Highlights

• •Lectins and glycan-binding antibodies are valuable as probe of glycans.
• •Advanced bioinformatics tools enable the mining of glycan-array data.
• •New insights into protein-glycan interactions have value in biological research.

     

Identification of Lysine Acetylation Sites on MERS-CoV Replicase pp1ab

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Highlights

• •Acetylation sites on MERS-CoV protein pp1ab were reported for the first time.
• •Sirt1 was predicted as upstream factor of identified acetylation events.

     

Potential use of microbial engineering in single-cell protein production

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Microbes of interest can be engineered to improve the production of SCPs for human-food and animal-feed applications. These improvements include feedstock conversion, biomass accumulation, protein production, and the production of nutritional and functional compounds.

     

An Improved Boosting to Amplify Signal with Isobaric Labeling (iBASIL) Strategy for Precise Quantitative Single-cell Proteomics

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Highlights

• •Higher AGC significantly improves quantitation quality in single-cell analysis.
• •The boosting-to-sample ratio should be carefully evaluated and optimized.
• •iBASIL allows for precise quantitation of 1,500 proteins from 104 AML single cells.
• •iBASIL recapitulates major biological differences in different AML single cells.

     

Phosphotyrosine-based Phosphoproteomics for Target Identification and Drug Response Prediction in AML Cell Lines

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Highlights

• •pY phosphoproteomes and dedicated ranking analyses for 16 AML cell lines.
• •RTK drivers, 6 mutant cell lines confirmed, identification for 4 more cell lines.
• •MAPK1/3 phosphorylation for cell lines without TK driver, indicating RAS mutation.
• •Drug target space phosphorylation correlates with drug IC50s in specific cell lines.

     

Selection of Features with Consistent Profiles Improves Relative Protein Quantification in Mass Spectrometry Experiments

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Highlights

• •Automated statistical approach for detecting uninformative features and outliers.
• •Improved performance on relative protein quantification.
• •An option in the open-source R-based software MSstats.

     

Parinarioidins A-B,unprecedented flavonoids from the bark of Brosimum parinarioides Ducke

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The Circadian Clock Gene Circuit Controls Protein and Phosphoprotein Rhythms in Arabidopsis thaliana

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Analytical Guidelines for co-fractionation Mass Spectrometry Obtained through Global Profiling of Gold Standard Saccharomyces cerevisiae Protein Complexes

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Highlights

• •Guidelines for studying protein complexes via co-fractionation mass spectrometry.
• •A novel procedure for profiling gold standard protein complexes in CF-MS data.
• •Recommendations for efficient CF-MS fractionation collection.
• •Scoring metric recommendations for precise and sensitive CF-MS data analysis.

     

Characterization of Prenylated C-terminal Peptides Using a Thiopropyl-based Capture Technique and LC-MS/MS

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Highlights

• •Brain membrane protein extraction.
• •Protein prenylation.
• •Prenyl peptide capture and characterization by LC-MS/MS.
• •HCD and EThcD peptide fragmentation.

     

BCKDK regulates the TCA cycle through PDC in the absence of PDK family during embryonic development

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Machine-learning-optimized Cas12a barcoding enables the recovery of single-cell lineages and transcriptional profiles

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Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain

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Altered Hippocampal-Prefrontal Neural Dynamics in Mouse Models of Down Syndrome

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Orientation Preference Maps in Microcebus murinus Reveal Size-Invariant Design Principles in Primate Visual Cortex

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A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis

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Engineering genetic circuits: advancements in genetic design automation tools and standards for synthetic biology

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The CD38/NAD/SIRTUIN1/EZH2 Axis Mitigates Cytotoxic CD8 T Cell Function and Identifies Patients with SLE Prone to Infections

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Layer 6b Is Driven by Intracortical Long-Range Projection Neurons

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Discovery of isonucleotidic CDNs as potent STING agonists with immunomodulatory potential

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