Sex-specific myoelectric manifestations of localized fatigue during a multi-joint repetitive task

We have previously demonstrated that fatigue at different locations impacts joint angles, angular variability, and coordination variability differently. However, the neuromuscular control aspects underlying these kinematic changes have never been demonstrated. Seventeen young adults (8 males) were recruited. Electromyographic electrodes were placed on: upper trapezius, pectoralis major, anterior and middle deltoid, biceps and triceps brachii, and left and right erector spinae. Subjects performed the repetitive pointing task (RPT) at 1 Hz for 30 s before and after localized fatigue tasks, which consisted of one shoulder, one elbow and one lower back isometric fatiguing protocols until exhaustion in randomized order. Electromyographic amplitude (RMS), variability (SD) and mean power frequency (MnPF) were calculated for each of the pre-fatigue and post-fatigue RPT trials. There were sex × fatigue location interaction effects on upper trapezius RMS (p = 0.038) with males’ values increasing the most after shoulder fatigue. Females’ triceps brachii RMS was greater compared to males after shoulder, elbow, and trunk fatigue (p = 0.003, p = 0.001 and p = 0.007 respectively). There were sex × fatigue location effects on left erector spinae MnPF (p = 0.011) with males and females’ values decreasing the most after trunk fatigue, but more so in males. Results demonstrate that males and females compensate differently during a repetitive pointing task when their elbows, shoulders and trunks are locally fatigued, which could have implications on sex-specific workplace injury risks. See Table 1 for acronyms.     

Mitochondrial Changes in Platelets Are Not Related to Those in Skeletal Muscle during Human Septic Shock

Platelets can serve as general markers of mitochondrial (dys)function during several human diseases. Whether this holds true even during sepsis is unknown. Using spectrophotometry, we measured mitochondrial respiratory chain biochemistry in platelets and triceps brachii muscle of thirty patients with septic shock (within 24 hours from admission to Intensive Care) and ten surgical controls (during surgery). Results were expressed relative to citrate synthase (CS) activity, a marker of mitochondrial density. Patients with septic shock had lower nicotinamide adenine dinucleotide dehydrogenase (NADH)/CS (p = 0.015), complex I/CS (p = 0.018), complex I and III/CS (p<0.001) and complex IV/CS (p = 0.012) activities in platelets but higher complex I/CS activity (p = 0.021) in triceps brachii muscle than controls. Overall, NADH/CS (r² = 0.00; p = 0.683) complex I/CS (r² = 0.05; p = 0.173), complex I and III/CS (r² = 0.01; p = 0.485), succinate dehydrogenase (SDH)/CS (r² = 0.00; p = 0.884), complex II and III/CS (r² = 0.00; p = 0.927) and complex IV/CS (r² = 0.00; p = 0.906) activities in platelets were not associated with those in triceps brachii muscle. In conclusion, several respiratory chain enzymes were variably inhibited in platelets, but not in triceps brachii muscle, of patients with septic shock. Sepsis-induced mitochondrial changes in platelets do not reflect those in other organs.     

The influence of contraction type,prior performance of a maximal voluntary contraction and measurement duration on fine-wire EMG amplitude

We aimed to investigate the impact of time on fine-wire (fw) electromyography (EMG) signal amplitude, and to determine whether any attenuation is confounded by task type. Twenty healthy participants were instrumented with fw and surface (s) EMG electrodes at the biceps brachii bilaterally. Participants held a weight statically with one arm and with the other arm either repeated the same task following a maximum voluntary contraction (MVC) or repeated dynamic elbow flexion/extension contractions. Each task was repeated for 30 s every five minutes over two hours. EMG amplitude was smoothed and normalized to time = 0. Stable median power frequency of the s-EMG ruled out the confounding influence of fatigue. Repeated-measures ANCOVAs determined the effect of electrode type and time (covariate) on EMG amplitude and the confounding impact of task type. During the isometric protocol, fw-EMG amplitude reduced over time (p = 0.002), while s-EMG amplitude (p = 0.895) and MPF (p > 0.05) did not change. Fw-EMG amplitude attenuated faster during the dynamic than the isometric protocol (p = 0.008) and there was evidence that the MVC preceding the isometric protocol impacted the rate of decline (p = 0.001). We conclude that systematic signal attenuation of fw-EMG occurs over time and is more pronounced during dynamic tasks.     

EMG amplitude and frequency parameters of muscular activity: Effect of resistance training based on electromyographic fatigue threshold

The present study aimed to evaluate the effect of a resistance training program based on the electromyographic fatigue threshold (EMG_FT, defined as the highest exercise intensity performed without EMG alterations), on the EMG amplitude (root mean square, RMS) and frequency (median frequency, MF) values for biceps brachii (BB), brachioradialis (BR), triceps brachii (TB) and multifidus (MT). Twenty healthy male subjects, (training group [TG], n = 10; control group [CG], n = 10), firstly performed isometric contractions, and after this, dynamic biceps curl at four different loads to determine the EMG_FT. The TG training program used the BB EMG_FT value (8 weeks, 2 sessions/week, 3 exhaustive bouts/session, 2 min rest between bouts). No significant differences were found for the isometric force after the training. The linear regression slopes of the RMS with time during the biceps curl presented significant decrease after training for the BB, BR and TB muscles. For the MT muscle, the slope and MF intercept values changed with training. The training program based on the EMG_FT influenced EMG the amplitude more than EMG frequency, possibly related to the recruitment patterns of the muscles, although the trunk extensor muscles presented changes in the frequency parameter, showing adaptation to the training program.     

Effects of electromyostimulation versus voluntary isometric training on elbow flexor muscle strength

The purpose of this study was to determine whether 7 weeks of standardized (same number and duration of repetitions, sets and rest strictly identical) electromyostimulation training of the elbow flexor muscles would induce strength gains equivalent to those of voluntary isometric training in isometric, eccentric and concentric contractions. Twenty-five males were randomly assigned to an electromyostimulated group (EMS, n = 9), a voluntary isometric group (VOL, n = 8), or a control group (CON, n = 8). Maximal voluntary isometric, eccentric and concentric strength, electromyographic (EMG) activity of the biceps and triceps brachii muscles, elbow flexor muscle activation (twitch interpolation technique) and contractile properties were assessed before and after the training period. The main findings were that the isometric torque gains of EMS were greater than those of VOL after the training period (P < 0.01) and that the eccentric and concentric torque gains were equivalent. In both groups, we observed that the mechanical twitch (Pt) was increased (P < 0.05) and that torque improvements were not mediated by neural adaptations. Considering the respective intensities of the training programs (i.e., submaximal contractions for EMS versus maximal for VOL), it can be concluded that electromyostimulation training would be more efficient than voluntary isometric training to improve both isometric and dynamic strength.     

A comparison of muscle activity of the dominant and non-dominant side of the body during low versus high loaded bench press exercise performed to muscular failure

The main aim of the study was to compare the peak surface electromyography (sEMG) amplitude of muscles during low and high loaded bench press exercises performed to muscular failure on the dominant and non-dominant body side. Ten resistance-trained healthy males with at least six-year experience in resistance training (27.7 ± 5.6 years, 81.1 ± 5.8 kg and 175.3 ± 5.2 cm, bench press one-repetition maximum [1RM] = 98.9 ± 7.1 kg) performed the bench press at 50% and at 90%1RM. The differences in peak sEMG amplitude between body-sides and the external loads were recorded for the pectoralis major (PM), anterior deltoid (AD), and the long head of the triceps brachii (TB) during each attempt. A two-way repeated-measures ANOVAs revealed statistically significant main effect of side for AD (p < 0.001) and TB (p < 0.001) but not for PM (p = 0.168) and a significant main effect of load for TB (p < 0.001) but not for AD and PM (p = 0.229; p = 0.072; respectively). The post-hoc analysis for the main effect of side showed significantly higher peak sEMG amplitude for the dominant side compared to the non-dominant side for AD and TB at 50%1RM and 90%1RM (p < 0.001; all) with no statistically significant differences for PM (p = 0.187; p = 0.155; both loads). The post-hoc analysis for the main effect of load for TB revealed a significantly higher peak sEMG amplitude at 90%1RM compared to the 50%1RM (p = 0.009). The obtained results indicate that regardless of the external load, the peak sEMG activity of the AD, PM, and TB during the bench press exercise performed to muscular failure was higher on the dominant body-side.     

Reduced plantar-flexors extensibility but improved selective motor control associated with age in young children with unilateral cerebral palsy and equinovalgus gait

BackgroundChildren with spastic cerebral palsy gradually lose muscle extensibility but the interplay between the muscular and neurological components of the condition is unclear especially in the pathophysiology of equinovalgus gait.AimThis study aimed to quantify the muscular and neurological disorders in young children with unilateral cerebral palsy, and to investigate the role of the peroneus longus (PL) in equinovalgus gait.Design, setting and population: This was an observational study with prospective assessments of 31 children (median age: 2.9 years, range: 2–6) from outpatient clinic in a tertiary teaching hospital.MethodsClinical measures of plantar flexor extensibility (X_V1), stretch response (X_V3), and active ankle dorsiflexion angle (X_A) were obtained as well as walking velocity and electromyography of tibialis anterior (TA), gastrocnemius medialis (GM) and PL during walking.ResultsWe found reduced extensibility of the triceps surae on the paretic side (effect size r = 0.73, p < 0.001 for soleus and r = 0.68, p < 0.001 for gastrocnemius) and a correlation between reduced triceps surae extensibility and earlier stretch response (ρ = 0.5, p = 0.004). During the swing phase, there was major co-contraction between TA and GM/PL, and significantly larger activation of PL compared to GM (r = 0.46, p = 0.011). Both GM and PL activation decreased with age.ConclusionsOur results suggest gradual deterioration of the muscular disorder and a link between the muscular and neurological disorders, although plantar flexor co-contraction improved with age. The PL was more activated than the GM and may be considered an intervention target to treat equinovalgus gait.     

Flow injection chemiluminescence determination of acetochlor and cartap-HCl in freshwater samples using an acidic KMnO4–rhodamine-B reaction system

A flow injection (FI) methodology using the acidic potassium permanganate (KMnO₄)–rhodamine-B (Rh-B) reaction with chemiluminescence (CL) detection was established to determine acetochlor and cartap-HCl pesticides in freshwater samples. Experimental parameters were optimized, and Chelex-100 cationic exchanger mini column and solid-phase extraction (SPE) were used as phase separation techniques. Linear calibration curves were observed for the standard solutions of acetochlor and cartap-HCl over the ranges 0.005–2.0 mg L⁻¹ [y = 1155.8x + 57.551, R² = 0.9999 (n = 8)] and 0.005–1.0 mg L⁻¹ [y = 979.76x + 14.491, R² = 0.9998 (n = 8)] with LODs and LOQs of 7.5 × 10⁻⁴ and 8.0 × 10⁻⁴ mg L⁻¹ (3σ blank) and 2.5 × 10⁻³ and 2.7 × 10⁻³ mg L⁻¹ (10σ blank), respectively, with an injection throughput of 140 h⁻¹. These methods were used to estimate acetochlor and cartap-HCl with or without the SPE procedure, respectively, in spiked freshwater samples. Results obtained were not significantly different at a 95% confidence level to those of other reported methods. Recoveries for acetochlor and cartap-HCl were obtained over the ranges 93–112% (RSD = 1.9–3.6%) and 98–109% (RSD = 1.7–3.8%), respectively. The most probable CL reaction mechanism was explored.     

Heat acclimation does not modify autonomic responses to core cooling and the skin thermal comfort zone

Exercise heat acclimation (HA) is known to magnify the sweating response by virtue of a lower threshold as well as increased gain and maximal capacity of sweating. However, HA has been shown to potentiate the shivering response in a cold-air environment. We investigated whether HA would alter heat loss and heat production responses during water immersion. Twelve healthy male participants underwent a 10-day HA protocol comprising daily 90-min controlled-hyperthermia (target rectal temperature, T_re 38.5 °C) exercise sessions. Preceding and following HA, the participants performed a maximal exercise test in thermoneutral conditions (ambient temperature 23 °C, relative humidity 50%) and were, following exercise, immersed in 28 °C water for 60 min. Thermal comfort zone (TCZ) was also assessed with participants regulating the temperature of a water-perfused suit during heating and cooling. Baseline pre-immersion T_re was similar pre- and post-HA (pre: 38.33 ± 0.33 °C vs post: 38.12 ± 0.36 °C, p = 0.092). The T_re cooling rate was identical pre-to post-HA (−0.03 ± 0.01 °C·min⁻¹, p = 0.31), as was the vasomotor response reflected in the forearm-fingertip temperature difference. Shivering thresholds (p = 0.43) and gains (p = 0.61) were not affected by HA. TCZ was established at similar temperatures, with the magnitude in regulated water temperature being 7.6 (16.3) °C pre-HA and 5.1 (24.7) °C post-HA (p = 0.65). The present findings suggest that heat production and heat loss responses during whole body cooling as well as the skin thermal comfort zone remained unaltered by a controlled-hyperthermia HA protocol.     

Effect of heat stress on superoxide anion production in native and crossbred cattle under in vitro whole blood culture model

Impact of global warming on the dairy industry has gained attention due to huge economic losses through low production and fertility caused by heat stress. Exposure to hyperthermia provokes a series of complex responses in mammals which are been related to morphological and physiological alterations including the production of reactive oxygen species (ROS). A quantitative spectrophotometric based nitroblue tetrazolium (NBT) reduction assay was used to estimate the superoxide anion (•O₂⁻) level in heat stressed (at 42 °C) whole blood cultures of native and crossbred bulls (Sahiwal and Frieswal), in vitro. The breed effect in the kinetics of •O₂⁻ production at different time periods of continual heat stress was analyzed by repeated measures ANOVA. Comparison between different time periods in reference to 37 °C was analyzed by paired t-test. The •O₂⁻ level was significantly different (p < 0.05) between cells at 37 °C and 42 °C at different periods of incubation. Kinetics study showed increment of •O₂⁻ production on the acute phase of stress followed by a reduction in both Sahiwal and Frieswal breeds. In Sahiwal breed, the inflated superoxide level continued abated till 4 h and raised again at 6 h, while in Frieswal •O₂⁻ level reverted to raise sooner with in 2 h of incubation itself. Contrarily, kinetic of •O₂⁻ level in plasma showed a significant reduction (p < 0.001) at 30 min of 42 °C incubation followed by increment of •O₂⁻ level. Further, the breed variation was significant (p < 0.05) and a significant high reduction of •O₂⁻ level was observed in Sahiwal breed. Our finding indicates that, a better and longer •O₂⁻ production homeostasis and higher plasma scavenging ability of native breed may be one of the reasons for the higher thermal tolerance of these breeds in tropical climate.     

Timing and Intensity of Light Correlate with Body Weight in Adults

Light exposure can influence sleep and circadian timing, both of which have been shown to influence weight regulation. The goal of this study was to evaluate the relationship between ambient light, sleep and body mass index. Participants included 54 individuals (26 males, mean age 30.6, SD = 11.7 years). Light levels, sleep midpoint and duration were measured with wrist actigraphy (Actiwatch-L) for 7 days. BMI was derived from self-reported height and weight. Caloric intake was determined from 7 days of food logs. For each participant, light and activity data were output in 2 minute epochs, smoothed using a 5 point (10 minute) moving average and then aggregated over 24 hours. The mean light timing above 500 lux (MLiT⁵⁰⁰) was defined as the average clock time of all aggregated data points above 500 lux. MLiT⁵⁰⁰ was positively correlated with BMI (r = 0.51, p<0.001), and midpoint of sleep (r = 0.47, p<0.01). In a multivariable linear regression model including MLiT⁵⁰⁰ and midpoint of sleep, MLiT⁵⁰⁰ was a significant predictor of BMI (B = 1.26 SE = 0.34, β = 0.53 p = 0.001, r ² _Δ = 0.22). Adjusting for covariates, MLiT⁵⁰⁰ remained an independent predictor of BMI (B = 1.28 SE = 0.36, β = 0.54, p = 0.002, r ² _Δ = 0.20). The full model accounted for 34.7% of the variance in BMI (p = 0.01). Exposure to moderate levels of light at biologically appropriate times can influence weight, independent of sleep timing and duration.     

Seasonal variations in mercury,cadmium, lead and arsenic species in Norwegian blue mussels (Mytilus edulis L.) – Assessing the influence of biological and environmental factors

BackgroundBlue mussels (Mytilus edulis L.) can accumulate undesirable substances, including the potentially toxic elements (PTEs) cadmium (Cd), mercury, (Hg), lead (Pb), arsenic (As) and As species. In this study, the levels of PTEs and As species were determined in samples of blue mussels to assess the influence of environmental and biological factors, and evaluate the potential risk associated with blue mussels in terms of food and feed safety.MethodologyBlue mussels were collected monthly from one location in Western Norway from February 2018 to December 2018, and from April 2019 to April 2020. Samples were analyzed for PTEs using inductively coupled plasma mass spectrometry (ICP-MS), and high-performance liquid chromatography (HPLC) coupled to ICP-MS. Temperature, salinity and fluorescence (chlorophyll a) were monitored in the seawater column by STD/CTD, to assess the potential influence of these environmental factors on the PTE levels in the mussels.ResultsThe results showed seasonal variations in the PTEs, with somewhat higher concentrations in spring and winter months. Unusually high levels of total As (101.2 mg kg^-1 dw) and inorganic As (53.6 mg kg^-1 dw) were observed for some of the time points. The organic As species arsenobetaine was generally the major As species (17–82% of total As) in the mussels, but also simple methylated As species and arsenosugars were detected. Principal components analysis (PCA) did not show a consistent relationship between the environmental factors and the PTE concentrations, showing contrary results for some elements for the periods studied. The condition index (CI) could explain variations in element concentration with significant correlations for Cd (r = −0.67, p = 0.009) and Pb (r = −0.62, p = 0.02 in 2019/20 and r = −0.52, p = 0.02 in 2018), whereas the correlation between As and CI was not significant (r = 0.12 in 2018, and r = −0.06 in 2019/20). Higher concentrations of iAs and arsenosugars coincided with increased signals of chlorophyll a, suggesting that phytoplankton blooms could be a source of As in the blue mussels.ConclusionTo our knowledge, this is the first study of As species in blue mussels collected over a time period of two years, providing an insight into the natural variations of these chemical forms in mussels. In terms of mussel as food and future feed material, concentrations of Cd, Hg and Pb were below the maximum levels (MLs) established in the EU food and feed legislation. However, levels of As and iAs in mussels at some time points exceeded the MLs for As in the feed legislation, and the margin of exposure (MOE) was low if these mussels were for human consumption, highlighting the importance of determining the chemical forms of As in feed and food.     

β2-microglobulin is overexpressed in buccal cells of elderly and correlated with expression of p16 and inflammatory genes

β2M (Beta 2 microglobulin) is a small protein that is found in all nucleated cells, previous finding showed that its levels increased in the serum of the elderly. Buccal cell samples are none invasive approach for assessing the expression of target genes. There was rationality to assess the expression of β2M in buccal cells of people of a different group of ages. Indeed, the expression of β2M increased significantly with fold change 3.40, 4.80, 6.60^**, 8.20^*** and 12.04^*** for the group of age 18–25 years, 26–35 years, 36–45 years, 46–55 years, and 56–70 years respectively. The same observation was seen with markers of biological aging (p16^INK4a) with fold change 3.19, 3.90, 4.80*, 8.50^*** and 12.40^*** for the group of age 18–25 years, 26–35 years, 36–45 years, 46–55 years, and 56–70 years respectively. As expected, there was an increase in the inflammatory genes (IL-1 β and IL-6) expression in the elderly. Moreover, there was a direct significant correlation (r = 90, p < 0.001) between β2M expression and age (years), and the same direct significant correlation between p16^INK4a expression and age (years) was also seen (r = 90, p < 0.001). In addition, a direct correlation between β2M and p16^INK4a was also seen (r = 0.8.3, p < 0.001), there was also direct correlation between β2M and IL-1 β and IL-6 with (r = 0.5, p < 0.001; r = 0.68, p < 0.001) respectively. This evidence showed that β2M increased in buccal cells of the elderly compared to younger, and thereby buccal cells can be exploited to assess biological aging by measuring β2M levels, however, large sample size and using another assessing method such as β2M protein levels should be performed to confirm the results.     

Assessing redistribution of muscle innervation zones after spinal cord injuries

This study aimed to examine the redistribution of neuromuscular junctions or innervation zones (IZs) after spinal cord injuries (SCI). Fifteen able-bodied subjects and 15 subjects with SCI (American Spinal Injury Association Impairment Scale A to D), participated in the study. Surface electromyography (EMG) signals were collected from the biceps brachii muscle by a customized linear electrode array when subjects generated maximal isometric voluntary contractions. The Radon transform was applied to detect the IZ locations in the multiple channel surface EMG signals which were differentiated between consecutive channels. The distribution of IZs was compared between the SCI and control groups using the student-t test. Statistical analysis disclosed a significantly wider range of IZs in the SCI group compared with the control group (SCI: 3.83 ± 1.32 IED, control: 2.83 ± 0. 87 IED, IED: inter-electrode distance, p < 0.05). No remarkable shifts of the center of the distribution were observed between the two groups (SCI: 9.23 ± 2.35 IED, control: 8.53 ± 2.33 IED, p = 0.42). Changes of IZ distribution in the paralyzed muscles could be associated with the complex neuromuscular reorganization after the SCI.     

Facilitation of triceps brachii muscle contraction by tendon vibration after chronic cervical spinal cord injury.

One way to improve the weak triceps brachii voluntary forces of people with chronic cervical spinal cord injury may be to excite the paralyzed or submaximally activated fraction of muscle. Here we examined whether elbow extensor force was enhanced by vibration (80 Hz) of the triceps or biceps brachii tendons at rest and during maximum isometric voluntary contractions (MVCs) of the elbow extensors performed by spinal cord-injured subjects. The mean +/- SE elbow extensor MVC force was 22 +/- 17.5 N (range: 0-23% control force, n = 11 muscles). Supramaximal radial nerve stimuli delivered during elbow extensor MVCs evoked force in six muscles that could be stimulated selectively, suggesting potential for force improvement. Biceps vibration at rest always evoked a tonic vibration reflex in biceps, but extension force did not improve with biceps vibration during triceps MVCs. Triceps vibration induced a tonic vibration reflex at rest in one-half of the triceps muscles tested. Elbow extensor MVC force (when >1% of control force) was enhanced by vibration of the triceps tendon in one-half of the muscles. Thus triceps, but not biceps, brachii tendon vibration increases the contraction strength of some partially paralyzed triceps brachii muscles.     

Maximal and submaximal isometric torque is elevated immediately following highly controlled active stretches of the hamstrings

Hamstring strain rehabilitation programs with an eccentric bias are effective but have a low adherence rate. Post-stretch isometric (PS-ISO) contractions which incorporate a highly controlled eccentric contraction followed by an isometric contraction resulting in elevated torque during following stretch, compared with isometric contractions at the same joint angle. This study measured torque, activation and musculotendinous unit behaviour of the hamstrings during PS-ISO contractions of maximal and submaximal levels using two stretch amplitudes. Ten male participants (24.6 years ± 2.22 years) completed maximal and submaximal baseline isometric contractions at 90°, 120° and 150° knee flexion and PS-ISO contractions of maximal and submaximal intensity initiated at 90° and 120° incorporating active stretch of 30° and 60° at 60°·s⁻¹. Torque and muscle activation of the knee flexors were simultaneously recorded. Musculotendinous unit behaviour of the biceps femoris long head was recorded via ultrasound during all PS-ISO contractions. Compared with baseline, torque was 8% and 39% greater in the maximal and submaximal PS-ISO conditions respectively with no change in muscle activation. The biceps femoris long head muscle lengthened during all PS-ISO contractions. PS-ISO contractions may be beneficial where the effects of highly controlled eccentric contractions and elevated isometric torque are desired, such as hamstring rehabilitation.     

Hamstring Muscle Fatigue and Central Motor Output during a Simulated Soccer Match

Purpose

To examine changes in hamstring muscle fatigue and central motor output during a 90-minute simulated soccer match, and the concomitant changes in hamstring maximal torque and rate of torque development.

Method

Eight amateur male soccer players performed a 90-minute simulated soccer match, with measures performed at the start of and every 15-minutes during each half. Maximal torque (Nm) and rate of torque development (RTD; Nm.s^–1) were calculated from maximal isometric knee flexor contractions performed at 10° of flexion. Hamstring peripheral fatigue was assessed from changes in the size and shape of the resting twitch (RT). Hamstring central motor output was quantified from voluntary activation (%) and normalized biceps femoris (BF) and medial hamstrings (MH) electromyographic amplitudes (EMG/M).

Results

Maximal torque was reduced at 45-minutes by 7.6±9.4% (p<0.05). RTD in time intervals of 0–25, 0–50, and 0–75 ms post-contraction onset were reduced after 15-minutes in the first-half between 29.6 to 46.2% (p<0.05), and were further reduced at the end of the second-half (p<0.05). Maximal EMG/M was reduced for biceps femoris only concomitant to the time-course of reductions in maximal torque (p = 0.007). The rate of EMG rise for BF and MH was reduced in early time periods (0–75 ms) post-contraction onset (p<0.05). No changes were observed for the size and shape of the RT, indicating no hamstring peripheral fatigue.

Conclusion

Centrally mediated reductions in maximal torque and rate of torque development provide insight into factors that may explain hamstring injury risk during soccer. Of particular interest were early reductions during the first-half of hamstring rate of torque development, and the decline in maximal EMG/M of biceps femoris in the latter stages of the half. These are important findings that may help explain why the hamstrings are particularly vulnerable to strain injury during soccer.     

Duration of differential activations is functionally related to fatigue prevention during low-level contractions

The aim of this study was to investigate the importance of duration of differential activations between the heads of the biceps brachii on local fatigue during prolonged low-level contractions. Fifteen subjects carried out isometric elbow flexion at 5% of maximal voluntary contraction (MVC) for 30 min. MVCs were performed before and at the end of the prolonged contraction. Surface electromyographic (EMG) signals were recorded from both heads of the biceps brachii. Differential activation was analysed based on the difference in EMG amplitude (activation) between electrodes situated at the two heads. Differential activations were quantified by the power spectral median frequency of the difference in activation between the heads throughout the contraction. The inverse of the median frequency was used to describe the average duration of the differential activations. The relation between average duration of the differential activations and the fatigue-induced reduction in maximal force was explored by linear regression analysis. The main finding was that the average duration of differential activation was positively associated to relative maximal force at the end of the 30 min contraction (R² = 0.5, P < 0.01). The findings of this study highlight the importance of duration of differential activations for local fatigue, and support the hypothesis that long term differential activations prevent fatigue during prolonged low-level contractions.     

Differential contributions of ankle plantarflexors during submaximal isometric muscle action: A PET and EMG study

The objective of this study was to investigate the relative contributions of superficial and deep ankle plantarflexors during repetitive submaximal isometric contractions using surface electromyography (SEMG) and positron emission tomography (PET). Myoelectric signals were obtained from twelve healthy volunteers (27.3 ± 4.2 yrs). A tracer ([¹⁸F]-FDG) was injected during the exercise and PET scanning was done immediately afterwards. The examined muscles included soleus (Sol), medial gastrocnemius (MG), lateral gastrocnemius (LG), and flexor hallucis longus (FHL). It was found that isometric maximal voluntary contraction (MVC) force, muscle glucose uptake (GU) rate, and SEMG of various plantarflexors were comparable bilaterally. In terms of %EMG MVC, FHL and MG displayed the highest activity (∼34%), while LG (∼21%) had the lowest activity. Cumulative SEMG from all parts of the triceps surae (TS) muscle accounted for ∼70% of the combined EMG signal of all four plantarflexors. As for GU, the highest quantity was observed in MG (2.4 ± 0.8 μmol * 100 g⁻¹ * min⁻¹), whereas FHL (1.8 ± 0.6 μmol * 100 g⁻¹ * min⁻¹) had the lowest uptake. Cumulative GU of TS constituted nearly 80% of the combined GU. The findings of this study provide valuable reference for studies where individual muscle contributions are estimated using models and simulations.     

Phylogeny,ecology and deep time: 2D outline analysis of anuran skulls from the Early Cretaceous to the Recent

Anurans have a long fossil record, spanning from the Early Jurassic to the Recent. However, specimens are often severely flattened, limiting their inclusion in quantitative analyses of morphological evolution. We perform a two‐dimensional morphometric analysis of anuran skull outlines, incorporating 42 Early Cretaceous to Miocene species, as well as 93 extant species in 32 families. Outlines were traced in tpsDig2 and analysed with elliptical Fourier analysis. Fourier coefficients were used in MANOVAs, phylogenetic MANOVAs (as significant phylogenetic signal was found) and disparity analyses across multiple ecological and life history groupings. The Neotropical realm showed higher disparity than the Australian, Palaearctic and Oriental realms (p = 0.007, 0.013, 0.038, respectively) suggesting concordance of disparity and diversity. Developmental strategy had a weak effect on skull shape (R² = 0.02, p = 0.039) and disparity was similar in metamorphosing and direct developing frogs. Ecological niche was a significant discriminator of skull shape (F = 1.43, p = 0.004) but not after phylogenetic correction. Evolutionary allometry had a small but significant influence on the cranial outlines of the combined extant and fossil dataset (R² = 0.05, p = 0.004). Finally, morphospace occupation appears to have changed over time (F = 1.59, p = 5 × 10^?10). However, as with ecological signal, this shift appears to be largely driven by phylogeny and was not significant after phylogenetic correction (R² = 0.26, p = 0.22). This study thus suggests that frog skull evolution is shaped more by phylogenetic constraints than by ecology.