Radiosensitizing activity of maleic acid derivatives. Effect of maleic acid o-nitroanilide, anilide and diethyl ester on the survival of gamma-irradiated cells of Escherichia coli B/r

Among three substances under study, only o-nitroanilide of maleic acid (oNAM) possessed a radiosensitizing activity. Radiosensitivity of E. coli B/r cells irradiated in the presence of Ar and oNAM was higher by 4.8 times than that of irradiated controls. The survival rate of E. coli B/r cells irradiated in the presence of oxygen was changed by 1.4 times by the effect of oNAM.     

新生儿缺血缺氧性脑病不同严重程度的MRI表现及其诊断价值分析

目的:比较新生儿缺血缺氧性脑病(HIE)不同严重程度的磁共振(MRI)和电子计算机断层扫描(CT)下的表现,分析MRI对新生儿HIE的诊断价值。方法:选择2017年1月至2019年8月我院收治的HIE新生儿76例,其中轻度46例,中度24例,重度6例,所有患儿均接受MRI和CT检查,比较MRI与CT对新生儿HIE的诊断结果、对新生儿HIE严重程度的诊断结果及对患儿颅内病变检出率的结果。结果:以临床表现为金标准,76例患儿经MRI检查确认为新生儿HIE的有72例,准确率为94.74%;经CT检查确认为新生儿HIE的有63例,准确率为82.89%,MRI检查对新生儿HIE的检出率显著高于CT检查(x2=5.365,P=0.021)。MRI检查对轻度、重度HIE诊断的符合率分别为71.74%(33/46)、100.00%(6/6),显著高于CT检查的43.48%(20/46)、50.00%(3/6)(均P0.05);MRI与CT对中度HIE诊断的符合率分别为75.00%(18/24)、66.67%(16/24),二者比较差异无统计学意义(x2=0.403,P=0.525)。MRI检查对脑室内出血、脑室周围出血、蛛网膜下腔出血、基底节损伤、脑水肿的总体检出率为98.68%(75/76),高于CT检查的90.79%(69/76)(x2=4.750,P=0.029)。结论:与CT相比,MRI能够更清晰显示新生儿的脑组织结构,对新生儿HIE诊断和严重程度的准确率优于CT,且对新生儿HIE脑组织病变情况的诊断也较CT更佳,具有较高的诊断价值。     

The effects of ionizing radiation on the peroxide content of a pure polyunsaturated lipid dispersion and of lipids and membranes derived from Acholeplasma laidlawii

Dispersions of a pure unsaturated phospholipid, dilinoleoylphosphatidyl choline, formed conjugated diene hydroperoxides when irradiated in air with 7 MeV electrons (150 Gy and 300 Gy). Peroxide formation was optimized when the dispersions were irradiated in air at 37 degrees C at a dose rate of 5 Gy/min. No significant loss of linoleic acid from the irradiated phospholipid dispersions was observed after doses of 150 or 300 Gy. Small amounts of thiobarbituric acid-reactive material were formed in irradiated unsaturated phospholipid dispersions. However, lipids or membranes isolated from 48 hour cultures of Acholeplasma laidlawii grown in media supplemented with either linoleic or linolenic acid did not appear to be peroxidized by irradiation under the same conditions.     

Effects of Gamma-irradiation on Lipid Metabolic Changes of Potato Tubers in Response to Mechanical Injury

Linolenic acid contents of glycolipids increased in irradiated potatoes during storage, accompanied by a decrease of linoleic acid. The puncturing of a potato tuber with a needle of a microsyringe caused the similar changes; the elevation of linolenic acid level and decline of linoleic acid were observed within 24 h after puncturing. Irradiation before the puncturing reduced the degree of the increase of linolenic acid in response to the mechanical injury. The rate of [¹³C]acetate incorporation into lipid fractions of irradiated tubers was smaller than that of unirradiated tubers, and polyunsaturated fatty acids (linoleic and linolenic acids) of lipid fractions were weakly labeled in irradiated tubers as compared with unirradiated ones. The results in this study indicate that irradiation retards lipid metabolism in response to mechanical injury.     

Evaluation of the effect of gamma rays on the venom of Vipera lebetina by biochemical study

Snake bites represent a serious public health problem in many areas of the world. In Algeria, two widespread snakes are Vipera lebetina and Cerastes cerastes. Vipera lebetina venom causes local hemorrhage and necrosis, and it may lead to permanent limb loss. The principal causes of mortality after snakebites are acute renal failure and hemorrhage, which occur not only locally, at the site of the bite, but also systemically, contributing to the cardiovascular shock characteristic of severe envenomation. Gamma radiation has been shown to be effective for attenuating venom toxicity. Vipera lebetina venom was irradiated with two doses of gamma rays (1 and 2 kGy) from a 60Co source, and the venom's toxic, enzymatic, and structural properties were analyzed. Intraperitoneal injection of the irradiated venoms (100-500 microg/20 g mouse body mass) revealed a significant decrease of the toxicity. Irradiated venoms with 1 and 2 kGy doses were four and nine times less toxic, respectively, than the native venom. A biochemical characterization of in vitro enzymatic activities was performed. Vipera lebetina displayed in vitro caseinolytic, amidolytic, esterasic, coagulant, and phospholipase A2 activities. Caseinolytic, amidolytic, esterasic, and coagulative activities were reduced for the irradiated venoms; only phospholipase A2 activity was abolished in the irradiated venom with a dose of 2 kGy. The native and irradiated venoms were separated by gel filtration and electrophoresis. Chromatographic and electrophoretic profiles were drastically changed as compared with the native venom. Vipera lebetina venom detoxified by gamma rays was used for active immunization, and the presence of antibody in the immune sera was detected by ELISA. The immunogenic properties were preserved and the antisera obtained with the irradiated venoms could cross-react. Antisera were able to neutralize the toxic effect of V. lebetina native venom. These results indicate that irradiation of V. lebetina venom with a dose of 2 kGy can promote a significant detoxification, keeping the immunological properties intact.     

Alpha Lipoic Acid Attenuates Radiation-Induced Thyroid Injury in Rats

Exposure of the thyroid to radiation during radiotherapy of the head and neck is often unavoidable. The present study aimed to investigate the protective effect of α-lipoic acid (ALA) on radiation-induced thyroid injury in rats. Rats were randomly assigned to four groups: healthy controls (CTL), irradiated (RT), received ALA before irradiation (ALA + RT), and received ALA only (ALA, 100 mg/kg, i.p.). ALA was treated at 24 h and 30 minutes prior to irradiation. The neck area including the thyroid gland was evenly irradiated with 2 Gy per minute (total dose of 18 Gy) using a photon 6-MV linear accelerator. Greater numbers of abnormal and unusually small follicles in the irradiated thyroid tissues were observed compared to the controls and the ALA group on days 4 and 7 after irradiation. However, all pathologies were decreased by ALA pretreatment. The quantity of small follicles in the irradiated rats was greater on day 7 than day 4 after irradiation. However, in the ALA-treated irradiated rats, the numbers of small and medium follicles were significantly decreased to a similar degree as in the control and ALA-only groups. The PAS-positive density of the colloid in RT group was decreased significantly compared with all other groups and reversed by ALA pretreatment. The high activity index in the irradiated rats was lowered by ALA treatment. TGF-ß1 immunoreactivity was enhanced in irradiated rats and was more severe on the day 7 after radiation exposure than on day 4. Expression of TGF-ß1 was reduced in the thyroid that had undergone ALA pretreatment. Levels of serum pro-inflammatory cytokines (TNF-α, IL-1ß and IL-6) did not differ significantly between the all groups. This study provides that pretreatment with ALA decreased the severity of radiation-induced thyroid injury by reducing inflammation and fibrotic infiltration and lowering the activity index. Thus, ALA could be used to ameliorate radiation-induced thyroid injury.     

Modulatory effect of Mentha piperita (Linn.) on serum phosphatases activity in Swiss albino mice against gamma irradiation

Mentha extract (ME; 1 g/kg body wt) given orally for three consecutive days prior to whole body irradiation (8 Gy) showed modulation of activity of serum phosphatases in albino mice. Values of acid phosphatase activities were significantly higher in untreated irradiated group throughout the experiment. Irradiated animals pretreated with ME showed significant decline in acid phosphatase activity as compared to untreated irradiated animals at all autopsy intervals and attained normalcy at day 5. A marked decrease in serum alkaline phosphatase activity was recorded in both irradiated groups. However, in ME pretreated irradiated group, values of alkaline phosphatase activity remained significantly higher than untreated irradiated animals at all intervals and attained normalcy from day 5 onwards.     

The role of UV‐irradiation pretreatment on the degradation of 2,4‐dichlorophenoxyacetic acid in water

The degradation of 2,4‐dichlorophenoxyacetic acid (2,4‐D) in water by the combination process of UV‐irradiation, humic acids and activated sludge treatment has been studied. The photoreaction rate of all irradiated samples was lowest for the sample irradiated at 308 nm (the XeCl excilamp) in the absence and in the presence of humic acids, and highest for the sample irradiated at 222 nm (the KrCl excilamp). Photolysis of 2,4‐D has been shown to enhance the subsequent microbial degradation. Copyright © 2010 John Wiley & Sons, Ltd.     

Effect of indometofen on the survival and bone marrow hemopoiesis of mice exposed to acute external gamma- or X-ray irradiation

The purpose of the study is evaluation of radioprotective effectiveness of indometofen at its prophylactic administration in conditions of acute irradiation. Evaluation of radioprotective efficiency was performed by studying the 30-day survival rate, life expectancy, structure of deathly irradiated mice, and bone marrow hemopoiesis using methods of endogenous and exogenous colony formation. The prophylactic application of indometofen at doses 30 mg/kg for 5 days before irradiation has been observed to protect mice against radiation death induced by gamma or X-ray exposures at doses LD(50-70/30), increasing their survival rate by 16-44%, and reduce severity of post radiation disorders of bone marrow hemopoiesis.     

Enhancement by tumor necrosis factor alpha of dengue virus-induced endothelial cell production of reactive nitrogen and oxygen species is key to hemorrhage development

Hemorrhage is a severe manifestation of dengue disease. Virus strain and host immune response have been implicated as the risk factors for hemorrhage development. To delineate the complex interplay between the virus and the host, we established a dengue hemorrhage model in immune-competent mice. Mice inoculated intradermally with dengue virus develop hemorrhage within 3 days. In the present study, we showed by the presence of NS1 antigen and viral nuclei acid that dengue virus actively infects the endothelium at 12 h and 24 h after inoculation. Temporal studies showed that beginning at day 2, there was macrophage infiltration into the vicinity of the endothelium, increased tumor necrosis factor alpha (TNF-alpha) production, and endothelial cell apoptosis in the tissues. In the meantime, endothelial cells in the hemorrhage tissues expressed inducible nitric oxide synthase (iNOS) and nitrotyrosine. In vitro studies showed that primary mouse and human endothelial cells were productively infected by dengue virus. Infection by dengue virus induced endothelial cell production of reactive nitrogen and oxygen species and apoptotic cell death, which was greatly enhanced by TNF-alpha. N(G)-nitro-L-arginine methyl ester and N-acetyl cysteine reversed the effects of dengue virus and TNF-alpha on endothelial cells. Importantly, hemorrhage development and the severity of hemorrhage were greatly reduced in mice lacking iNOS or p47(phox) or treatment with oxidase inhibitor, pointing to the critical roles of reactive nitrogen and oxygen species in dengue hemorrhage.     

曲酸产生菌激光诱变效应的研究

采用经紫外线(UV)、^60Co、亚硝基胍(NTG)复合诱变得到的黄曲霉曲酸产生菌(UCN7—12),进行激光诱变处理。研究证实在经过UV、^60Co、NTG诱变处理后,黄曲霉突变株用He—Ne激光与YAG激光进行诱变处理仍能提高产酸率,其中He—Ne激光辐照处理20min,正变率为12．1％,产量提高约13％。YAG激光辐照处理300sec,正变率16．7％,产量提高18．3％。说明上述两种激光对黄曲霉曲酸产生菌有一定的诱变效应。     

Role of the Phagocyte in Host-Parasite Interactions VIII. Effect of Whole-Body X Irradiation on Nicotinamides, Lysosomal Enzymes, and Bactericidal Activities of Leukocytes During Phagocytosis

By studying the effects of whole-body X irradiation on phagocytosis, a correlation between the metabolic and bactericidal activities of leukocytes following X irradiation was demonstrated. The total nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP) content of polymorphonuclear neutrolphils (PMN) isolated from irradiated guinea pigs increased significantly when compared to nonirradiated controls. The ratio of unreduced to reduced (NAD) generally increased in PMN isolated from irradiated animals. This occurred with both resting and phagocytizing cells. The ratio of unreduced to reduced NADP of resting PMN isolated from irradiated animals had a tendency to increase. However, in phagocytizing cells a significant decrease in the ratio was noted. The total acid and alkaline phosphatase and beta-glucuronidase increased up to about 10 days postirradiation. These lysosomal enzymes returned to approximately normal by the 17th day postirradiation. All three lysosomal enzymes (acid and alkaline phosphatases and beta-glucuronidase) were released from the granules at a significantly faster rate during phagocytosis after irradiation. The bactericidal activities of PMN isolated from irradiated animals gradually decreased, and in some cases increased growth of the organisms was observed. The uptake or association of bacteria with PMN isolated from irradiated animals varied with the postirradiation time. Generally, a correlation with bactericidal activities could be made. The data indicate that the bactericidal system in phagocytes consists of at least two agents, H(2)O(2) and myeloperoxidase.     

MRI-Based Analysis of Intracerebral Hemorrhage in Mice Reveals Relationship between Hematoma Expansion and the Severity of Symptoms

Intracerebral hemorrhage (ICH) is featured by poor prognosis such as high mortality rate and severe neurological dysfunction. In humans, several valuables including hematoma volume and ventricular expansion of hemorrhage are known to correlate with the extent of mortality and neurological dysfunction. However, relationship between hematoma conditions and the severity of symptoms in animal ICH models has not been clarified. Here we addressed this issue by using 7-tesla magnetic resonance imaging (MRI) on collagenase-induced ICH model in mice. We found that the mortality rate and the performance in behavioral tests did not correlate well with the volume of hematoma. In contrast, when hemorrhage invaded the internal capsule, mice exhibited high mortality and showed poor sensorimotor performance. High mortality rate and poor performance in behavioral tests were also observed when hemorrhage invaded the lateral ventricle, although worsened symptoms associated with ventricular hemorrhage were apparent only during early phase of the disease. These results clearly indicate that invasion of the internal capsule or the lateral ventricle by hematoma is a critical determinant of poor prognosis in experimental ICH model in mice as well as in human ICH patients. MRI assessment may be a powerful tool to refine investigations of pathogenic mechanisms and evaluations of drug effects in animal models of ICH.     

Spinacia oleracea L. protects against gamma radiations: a study on glutathione and lipid peroxidation in mouse liver

The present study deals with the protective effect of Spinacia oleracea L. against radiation-induced oxidative stress, which is evaluated in terms of lipid peroxidation (LPO) product and tissue levels of glutathione. Swiss albino male mice aged 6–8 weeks, weighing 22±3 g, each were selected from an inbred colony and divided into four groups. One group served as normal and a second group (extract of S. oleracea L. (SE) treated un-irradiated) were administered methanolic (50%) SE at a dose of 1100 mg/kg body wt./day dissolved in distilled water. A third group (untreated-irradiated) was administered distilled water orally, which served as control. A fourth group (SE pre-treated irradiated) was administered methanolic (50%) SE at a dose of 1100 mg/kg body wt./day dissolved in distilled water. Two groups, one untreated-irradiated and another S. oleracea pre-treated irradiated were exposed to 5 Gy of gamma radiation at a rate of 1.07 Gy/min with a source-to-surface distance of 77.5 cm. The animals were autopsied at 1, 3, 7, 15, and 30 days post-exposure. LPO increased after irradiation up to day 15 in the untreated-irradiated group and up to day 7 in SE pre-treated irradiated mice. LPO values were significantly lower in the SE pre-treated irradiated group as compared to their respective untreated-irradiated group at all intervals, which reached normal values from day 7 onward. The percentage of protection observed in the SE pre-treated irradiated group was, 22.22%, 24.8%, 33.25%, 42.84% and 26.36% at 1, 3, 7, 15, 30 days post-exposure, respectively. Radiation-induced glutathione depletion was checked after 7 days’ exposure in SE pre-treated irradiated as compared to untreated-irradiated in which recovery started after day 15. Values were significantly higher in the SE pre-treated irradiated group from their respective untreated-irradiated group at all intervals. The percentage of protection observed in the SE pre-treated irradiated group was, 29.41%, 42.68%, 43.55%, 53.81%, 39.28% at 1, 3, 7, 15, 30 days post-exposure, respectively. It is found that radiation-induced augmentation in malondialdehyde contents and depletion in glutathione changes in liver can be altered by S. oleracea L. The protection may be attributed to the combined effects of its constituents rather than to any single factor as the leaves are rich in carotenoid content (β-carotene, lutein, Zeaxanthine), ascorbic acid, flavonoids and p-coumaric acid. Thus Spinacia, showing protection in liver, may prove promising as a rich source of antioxidants because its use is cost-effective, especially for peoples in adverse and hazardous circumstances who are living in poverty.     

血清转化生长因子beta-1 在大鼠放射性肝纤维化中的动态表达 及其相关性研

目的:肝癌的放射治疗可导致放射性肝损伤(RILD)、甚至肝纤维化及肝硬化等并发症的发生,因此寻找较佳的血清标记物对放射性肝纤维化的无创诊断及监测具有重要意义。本文通过建立放射性肝纤维化大鼠模型,检测血清转化生长因子β1(TGF-β1)的动态表达,从而探讨其与放射性肝纤维化严重程度的相关性及其作为血清标记物的诊断价值。方法:雄性SD大鼠40只,随机分为模型组(30只)和对照组(10只)。除对照组外,模型组大鼠右半肝均接受单次6MV X线25Gy照射,于照射2月、4月、6月后,随机抽取10只,酶联免疫吸附法(ELISA)检测血清TGF-β1的表达,同时将大鼠肝组织进行HE染色,观察肝组织病理变化及大鼠肝纤维化程度,将后者与血清TGF-β1值进行相关性分析。结果:在照射第2月、4月、6月后,模型组大鼠血清TGF-β1值(分别为551.03±69.00 ng/L、645.31±109.29 ng/L、737.89±118.11 ng/L)逐渐升高,均明显高于对照组(451.71±51.12 ng/L,P<0.05)。通过相关性分析表明,大鼠血清TGF-β1值与肝纤维化程度正相关(r=0.82,P<0.01)。结论:在放射性肝纤维化发生发展中,TGF-β1随着肝纤维化严重程度增加,其表达亦升高。本研究为放射性肝纤维化严重程度的监测提供了一种新的无创、操作简单的手段,为后续TGF-β1作为血清标记物应用于放射性肝纤维化的临床研究奠定了理论基础。     

Leptin as a marker for severity and prognosis of aneurysmal subarachnoid hemorrhage

Leptin has been identified as a plasma marker for outcomes in traumatic brain injury and intracerebral hemorrhage. We further investigated whether leptin might serve as a marker for severity and prognosis in aneurysmal subarachnoid hemorrhage. One hundred and eight consecutive patients and 108 sex and age – matched healthy subjects were recruited. Plasma leptin levels were measured by enzyme-linked immunosorbent assay. Clinical severity was assessed using World Federation of Neurological Surgeons score and Fisher score. Mortality and poor long-term outcome (Glasgow outcome scale scores of 1–3) at 6 months were recorded. Plasma leptin levels on admission were substantially higher in patients than in healthy controls, and were significantly associated with the clinical severity. There was also a significant association between leptin levels and clinical outcomes at 6 months in multivariate logistic regression analysis. Using receiver operating characteristic curves, we calculated areas under the curve for clinical outcomes at 6 months. The predictive performance of leptin was similar to, but did not obviously improve those of World Federation of Neurological Surgeons score and Fisher score. Thus, leptin may indicate clinical severity of the initial bleeding and also have prognostic value for clinical outcomes in aneurysmal subarachnoid hemorrhage and may therefore help in guiding treatment decisions in the setting of aneurysmal subarachnoid hemorrhage.     

Repair of Irradiated Transforming Deoxyribonucleic Acid in Wild Type and a Radiation-Sensitive Mutant of Micrococcus radiodurans

The survival of biological activity in irradiated transforming deoxyribonucleic acid (DNA) has been assayed in the wild type and a radiation-sensitive mutant of Micrococcus radiodurans. The frequency of transformation with unirradiated DNA was lower in the mutant to about the same extent as the mutant's increased sensitivity to radiation. However, in both the wild type and the mutant, the irradiated DNA that was incorporated into the bacterial genome was repaired to the same extent as determined by the loss of transforming activity with increasing radiation dose. This applied to DNA irradiated either with ionizing or ultraviolet (UV) radiation. The rate of inactivation of biological activity after UV radiation was the same in any of the DNA preparations tested. For ionizing radiation, the rate of inactivation varied up to 40-fold, depending on the DNA preparation used, but for any one preparation was the same whether assayed in the wild type or the radiation-sensitive mutant. When recipient bacteria were irradiated with ionizing or UV radiation immediately before transformation, the frequency of transformation with unirradiated DNA fell, rapidly and exponentially in the case of the sensitive mutant but in a more complicated fashion in the wild type. The repair of DNA irradiated with ionizing radiation was approximately the same whether assayed in unirradiated or irradiated hosts. Thus, irradiation of the host reduced the integration of DNA but not its repair.     

Selective decomposition of either enantiomer or aspartic acid irradiated with60Co-γ-rays in the mixed aqueous solution with D- or L-alanine

Aqueous solutions of various amino acids were irradiated with⁶⁰Co--rays, and subsequently the remaining amino acids were analyzed using HPLC. The D₃₇ for the 1 mM glycine and alanine solutions were 1.95×10⁴ and 1.48×10⁴ Gy, respectively. However, when the mixed solutions of glycine and alanine (each in 0.5 mM) were irradiated under the identical condition, the D₃₇ for the glycine decomposition increased to 3.56×10⁴ Gy, while that for alanine decreased to 0.65×10⁴ Gy. A similar phenomenon was observed also in the case of the mixed solutions of aspartic acid and alanine. Namely, aspartic acid was protected from the attack of radiation by the presence of alanine in the solutions. The most interesting finding in this combination experiment is that, when D,L-aspartic acid was irradiated in the presence of L-alanine, the radiation-sensitivity of L-aspartic acid decreased selectively and vice versa. Namely, the asymmetric field induced in the solutions by adding D- or L-alanine might affect the radiodecomposition rate of either aspartic acid. Addition of glycine to D,L-aspartic acid did not bring about the asymmetric decomposition. It seems that some interaction between these amino acid molecules resulted in this effect.     

Effect of the superoxide anion (O2-) on Na+-dependent amino acid transport

Superoxide anion (O2-) generated either by the autoxidation of dihydroxyfumaric acid (DHF) or enzymatically by the xanthine-xanthine oxidase system inhibited the uptake of 2-aminoisobutyric acid (AIB) in thymocytes. The transport of this non-metabolizable amino acid in thymocytes is mediated by a Na+-dependent mechanism. Inhibition of this transport system by O2- was similar to that observed when radiosensitive lymphocytes are subjected to ionizing radiation. As in irradiated thymocytes, O2- generation affected primarily the maximal rate of uptake of the amino acid (i.e. Vmax). No change was observed in the apparent affinity of the amino acid for its carrier (i.e. Km) or the efflux rate of the amino acid. The data suggests that the superoxide anion may be one of the major species responsible for the observed radiation damage to radiosensitive lymphoid cells.     

Detection of Cerebral Hemorrhage in Rabbits by Time-Difference Magnetic Inductive Phase Shift Spectroscopy

Cerebral hemorrhage, a difficult issue in clinical practice, is often detected and studied with computed tomography (CT), magnetic resonance imaging (MRI), and positron emission tomography (PET). However, these expensive devices are not readily available in economically underdeveloped regions, and hence are unable to provide bedside and emergency on-site monitoring. The magnetic inductive phase shift (MIPS) is an emerging technology that may become a new tool to detect cerebral hemorrhage and to serve as an inexpensive partial substitute to medical imaging. In order to study a wider band of cerebral hemorrhage MIPS and to provide more useful information for measuring cerebral hemorrhage, we established a cerebral hemorrhage magnetic induction phase shift spectroscopy (MIPSS) detection system. Thirteen rabbits with five cerebral hemorrhage states were studied using a single coil-coil within a 1 MHz-200 MHz frequency range in linear sweep. A feature band (FB) with the highest detection sensitivity and the greatest stability was selected for further analysis and processing. In addition, a maximum conductivity cerebrospinal fluid (CSF) MRI was performed to verify and interpret the MIPSS result. The average phase shift change induced by a 3 ml injection of autologous blood under FB was -7.7503° ± 1.4204°, which was considerably larger than our previous work. Data analysis with a non-parametric statistical Friedman M test showed that in the FB, MIPSS could distinguish the five states of cerebral hemorrhage in rabbits, with a statistical significance of p<0.05. A B-F distribution profile was designed according to the MIPSS under FB that can provide instantaneous diagnostic information about the cerebral hemorrhage severity from a single set of measurements. The results illustrate that the MIPSS detection method is able to provide a new possibility for real-time monitoring and diagnosis of the severity of cerebral hemorrhage.