浮毛茛的组织培养与植株再生

1植物名称浮毛茛(Ranunculus natans C.A.Mey.),又称水毛。2材料类别基生茎。3培养条件愈伤组织诱导培养基:(1)MS 6-BA 1mg·L~(-1)(单位下同);(2)MS 6-BA 1 IBA 0.6:(3)MS 6-BA 1 IAA 0.6:(4)MS 6-BA 1 2,4-D 0.6;(5)MS 6-BA 1 NAA 0.6。愈伤组织分化培养基:(6)1/2MS 6-BA 0.2 NAA 0.1;(7)1/2MS 6-     

独尾草的组织培养与植株再生

1植物名称独尾草(EremuruschinensisFedtsch.)。2材料类别根。3培养条件愈伤组织诱导培养基:(1)MS+6-BA2.0mg·L-1(单位下同)+2,4-D0.5;(2)MS+6-BA3.0+NAA1.0。增殖及苗诱导培养基:(3)MS+6-BA2.0￣3.0+NAA0.5;(4)MS+6-BA1.0+KT0.2+NAA0.02;(5)MS+6-BA3.0+NAA0.05。生根培养基:(6)MS+IBA0.1;(7)MS+NAA0.1。所用培养基均加0.8%￣0.9%琼脂、3%白砂糖,pH5.6￣5.8。培养温度为(22±2)℃,相对湿度65%￣75%,光照时间12￣14h·d-1,光强30￣40μmol·m-2·s-1。4生长与分化情况4.1愈伤组织的获得选取秦岭(陕西省略阳县)野生独尾…     

海滨锦葵的组织培养和植株再生

1植物名称海滨锦葵[Kosteletzkya virginica (L.) K.PreslexGray]。2材料类别无菌苗茎段。3培养条件(1)愈伤组织诱导培养基为MS+KT1.5mg·L-1(单位下同)+IAA2.0或MS+6-BA4.0+IBA0.2,以在培养基中加入50mg·L-1的肌醇效果较     

枫叶秋海棠的组织培养与植株再生

1植物名称枫叶秋海棠(Begonia heracleifolia Cham.et Schlechtend.)。2材料类别叶片。3培养条件(1)叶片愈伤组织诱导培养基:MS 6-BA 0.2 mg·L~(-1)(单位下同) IAA 0.5;(2)丛生芽诱导培养基:MS 6-BA 0.5 IAA 0.2;(3)生根培养基:1/2MS NAA 0.2～0.4。以上培养基均加入6.     

霸王的组织培养和植株再生

1植物名称霸王[Zygophyllun xanthoxylun(Bunge) Maxim.]。2材料类别当年生健壮枝条。3培养条件芽诱导培养基:(1)MS 6-BA 0.5 mg·L~(-1)(单位下同) NAA 0.01;继代增殖培养基:(2)MS 6-BA 0.3 NAA 0.5;生根培养基:(3) MS IAA 1.0。以上培养基均附加3%蔗糖(生根培     

连钱草的组织培养与植株再生

1植物名称连钱草[Glechomalongituba(Nakai)Kupr.]。2材料类别叶片和茎段(带侧芽)。3培养条件诱导芽培养基:(1)MS+NAA0.2mg·L-1(单位下同),(2)MS+NAA0.2+6-BA1.0,(3)MS+NAA0.2+6-BA2.0,(4)MS+NAA0.2+6-BA3.0;增殖培养基:(5)MS+6-BA2.0￣4.0;生根培养基:(6)1/2MS+IBA0.5+IAA0.2。各培养基中均添加30g·L-1白糖和5g·L-1琼脂,pH5.8。培养温度25℃,诱导和增殖培养的光强为1000￣2000μmol·m-2·s-1,光照时间12h·d-1。4生长与分化情况4.1启动培养取连钱草植株,清洗干净,截取茎段(带侧芽),用70%的酒精处理30s,转入0.1%升汞…     

翻白草的组织培养和植株再生

1植物名称翻白草(Potentilla discolor Bunge)。2材料类别叶片。3培养条件(1)愈伤组织诱导培养基:MS 6-BA 1 mg·L~(-1)(单位下同) 2,4-D 0.5、1、2、3、4;(2)诱导芽分化培养基:MS 6-BA 1 NAA 0.5、1、2、3、4;(3)诱导生根培养基:1/2MS IBA 0.5、1、2、3、4。培养基均加30 g·L~(-1)蔗糖、     

沙地植物柄扁桃的组织培养与植株再生

1植物名称柄扁桃[Prunuspedunculata(Pall.)Maxim.],别名长柄扁桃。2材料类别实生苗茎尖。3培养条件不定芽诱导培养基:(1)MS+6-BA2.0mg·L-1(单位下同)+NAA0.1;(2)MS+6-BA2.0+NAA0.2;(3)MS+6-BA1.0+NAA0.1;(4)MS+6-BA1.0+NAA0.2。壮苗与生根培养基:(5)1/2MS+IBA0.5。上述培养基均加5.0g·L-1的琼脂粉和3%蔗糖,pH5.8。培养温度为20～25℃,光强为30～40μmol·m-2·s-1,光照时间为12～14h·d-1。4生长与分化情况4.1无菌苗的培育取饱满种子,去掉内果皮,用流水冲洗12h,用0.1%HgCl2消毒10min,无菌水冲洗4次,接种于琼脂培养基上…     

三叶香茶菜的组织培养及植株再生

1植物名称三叶香茶菜[Rabdosia ternifolia（D．Don）Hara]。 2材料类别带腋芽茎段。 3培养条件（1）诱导愈伤组织的培养基：MS＋6．BA2mg.L^-1（单位下同）＋NAA0．5;（2）芽分化及继代培养基：MS＋6．BA2＋IBA0．5：（3）诱导生根培养基：1／2MS＋IBA0．5＋NAA0．1。     

散斑肖万寿竹的组织培养与植株再生

1植物名称散斑肖万寿竹[Disporopsis aspera (Hua) Engl.exKrause],又名散斑竹根七。2材料类别带节茎段3培养条件诱导培养基:(1)MS 6-BA1.5mg·L-1(单位下同) 2,4-D0.5 NAA0.05;增殖培养基:     

Ultrastructure of Oogenesis in Dryopteris crassirhizoma Nakai

The ultrastructure of oogenesis in Dryopteris crassirhizoma Nakai has been investigated using transmission electron microscopy. The nucleus in the young egg is rounded with an uneven outline. As it develops, it becomes amoeboid and extends nuclear protrusions that are not only sac-like nuclear evaginations like those often seen in the oogenesis of other ferns, but also mushroom-like and finger-like, with an opening at their end allowing the nucleolus material to flow out from the openings. This has not been observed previously. The nuclear protrusions differ from Dryopteris filix-mas (L.) Schott. in the absence of sheets of nuclear membrane in the form of a closed ring. As the egg matures, the nucleus transforms into a tuber-like structure with a smooth surface, lying transversely in the egg cell. In the immature egg, vesicles almost encircle the nucleus twice and are most remarkable. In the maturing egg, the vesicles are distributed at the periphery, except for at the top of the egg, and affect the formation of the separation cavity and extra egg membrane. Simultaneously, vesicles from the venter canal cell move to the egg and take part in the formation of separation cavity and extra egg membrane. In the mature egg, a large number of small vesicles containing fragments of lamellae or osmiophilic material emerge from the cytoplasm. The origin of these vesicles is obscure. Irregular plastids containing a cylindrical starch grain dedifferentiated progressively.Mitochondria seem to have been undeveloped during the process, but return to normal at later stages of oogenesis. There is a high frequency of ribosomes in the mature egg. Microtubules, rarely seen in the eggs ofD. filix-mas (L.) Schott. and Pteridium aquilinum (L.) Kuhn, have been observed inside the plasmalemma of the maturing egg in D. crassirhizoma.     

Ultrastructure of Oogenesis in Dryopteris crassirhizoma Nakai

The ultrastructure ofoogenesis in Dryopteris crassirhizoma Nakai has been investigated using transmission electron microscopy. The nucleus in the young egg is rounded with an uneven outline. As it develops, it becomes amoeboid and extends nuclear protrusions that are not only sac-like nuclear evaginations like those often seen in the oogenesis of other ferns, but also mushroom-like and finger-like, with an opening at their end allowing the nucleolus material to flow out from the openings. This has not been observed previously. The nuclear protrusions differ from Dryopterisfilix-mas (L.) Schott. in the absence of sheets of nuclear membrane in the form of a closed ring. As the egg matures, the nucleus transforms into a tuber-like structure with a smooth surface, lying transversely in the egg cell. In the immature egg, vesicles almost encircle the nucleus twice and are most remarkable. In the maturing egg, the vesicles are distributed at the periphery, except for at the top of the egg, and affect the formation of the separation cavity and extra egg membrane. Simultaneously, vesicles from the venter canal cell move to the egg and take part in the formation of separation cavity and extra egg membrane. In the mature egg, a large number of small vesicles containing fragments of lamellae or osmiophilic material emerge from the cytoplasm. The origin of these vesicles is obscure. Irregular plastids containing a cylindrical starch grain dedifferentiated progressively.Mitochondria seem to have been undeveloped during the process, but return to normal at later stages of oogenesis. There is a high frequency of ribosomes in the mature egg. Microtubules, rarely seen in the eggs of D.filix-mas (L.) Schott. and Pteridium aquilinum (L.) Kuhn, have been observed inside the plasmalemma of the maturing egg in D. crassirhizoma.     

粗茎鳞毛蕨应用的研究

为开发利用粗茎鳞毛蕨(Dryopteris crassirhizoma Nakai)的药用和观赏价值、提高其质量和产量,对其进行了人工有性繁殖和复壮研究.在大批量人工有性育苗的基础上,予以分组复壮、对照栽培.筛选出了经济简便的技术路线,观察了形态发育与栽培条件的相关性.     

鲁梅克斯的组织培养和植株再生

1植物名称鲁梅克斯K-1杂交酸模(Rumexpatientia×Rumex tianschanicus). 2材料类别幼嫩花序. 3培养条件培养基为:(1)诱导和增殖培养基:MS 6-BA 1 mg·L-1(单位下同);(2)生根培养基:1/2MS NAA 0.2.培养基(1)、(2)附加200 mg·L-1水解酪蛋白、30 g·L-1蔗糖、6.5g·L-1琼脂,pH 5.8～6.0,120℃灭菌20 min.培养温度为(26±2)℃,光照8～10 h·d-1,光照度为800～1000lx.     

白蜡树属植物的组织培养和植株再生

从形态发生的不同途径,就有关白蜡树属植物的器官发生、腋芽增殖和体细胞胚胎发生植株再生,以及每一形态发生的培养过程和影响因素的研究进展作了介绍和展望.     

多年生黑麦草的组织培养和植株再生

1植物名称多年生黑麦草(Lolium perenne)栽培品种"德比"(Derbv). 2材料类别成熟种子. 3培养条件(1)愈伤组织诱导培养基:N6 2,4-D10.0 mg·L-1(单位下同) 6-BA 0.5 NAA 0.5 CH(水解酪蛋白)500;(2)继代培养基:N6 2,4-D 5.0 NAA 0.5 6-BA 0.5 CH 300;(3)分化培养基:N6 6-BA 1.0 KT 0.5;(4)壮苗培养基:N6;(5)生根培养基:1/2N6 NAA 0.2.以上培养基中除生根培养基蔗糖含量为3%以外,其余均为5%.pH 5.8,琼脂含量为6.5%.愈伤组织诱导及继代为暗培养;分化培养及生根培养的光照度均为1800 1x,光照时间12 h·d-1,培养温度(25 2)℃.     

Dammaradiene synthase, a squalene cyclase, from Dryopteris crassirhizoma Nakai

Ferns produce a variety of cyclic triterpene hydrocarbons in large amount. Squalene cyclases (SCs) are responsible enzymes for formation of cyclic triterpene hydrocarbon skeletons. Although more than ten bacterial SCs have been cloned and four of them characterized for their enzymatic products, the only example of a fern SC is ACH, from Adiantum capillus-veneris, which produces hydroxyhopane. To obtain a deeper understanding of the molecular evolution of SCs and the origin of the structural diversity of fern triterpenes, further cloning and characterization of SCs have been pursued. In this study, a SC cDNA, DCD, was cloned from Dryopteris crassirhizoma by homology-based RT-PCR. DCD contains a 2058-bp open reading frame that encodes a 685 amino acid polypeptide exhibiting 66% identity to the previously identified fern SC, ACH, and 35-40% identity to bacterial SCs. Heterologous expression of DCD in yeast established it to be a dammaradiene synthase affording dammara-18(28),21-diene, a tetracyclic triterpene hydrocarbon. Although neither this compound nor any derived metabolites have been previously reported from D. crassirhizoma, re-investigation of the leaflets demonstrated the presence of dammara-18(28),21-diene. DCD represents the first SC that produces a tetracyclic triterpene hydrocarbon.     

麻疯树的组织培养及植株再生

1　植物名称　麻疯树 (Ｊａｔｒｏｐｈａｃｕｒｃａｓ)。2　材料类别　无菌萌发的种子苗 (种子采自四川省攀枝花 )。3　培养条件　基本培养基为ＭＳ。无菌苗萌发培养基 :( 1 )ＭＳ 6 ＢＡ 5 .0ｍｇ·Ｌ- 1 (单位下同 ) ＩＢＡ 0 .1 ;( 2 )ＭＳ 6 ＢＡ 2 .5 ＩＢＡ 0 .1 ;     

甜瓜的组织培养与植株再生

1　植物名称　甜瓜 (Ｃｕｃｕｍｉｓｍｅｌｏ)品种“伽师瓜”。2　材料类别　成熟甜瓜种子无菌苗的子叶。3　培养条件　种子萌发培养基 :(1 ) 1 / 2ＭＳ(大量元素减半 ) 2 %蔗糖。愈伤组织诱导培养基 :(2 )Ｍｉｌｌｅｒ 6 ＢＡ 5 .0ｍｇ·Ｌ- 1 (单位下同 ) ＺＴ 0 .0 1 3 %蔗糖。丛生芽诱导培养基 :(3 )Ｍｉｌｌｅｒ 6 ＢＡ 2 .0 3 %蔗糖 ;(4)Ｍｉｌｌｅｒ 6 ＢＡ 0 .5 3 %蔗糖。芽伸长培养基 :(5 )Ｍｉｌｌｅｒ 6 ＢＡ 0 .1 3 %蔗糖 ;(6 )Ｍｉｌｌｅｒ 6 ＢＡ 0 .0 1 3 %蔗糖。生根培养基 :(7) 1 /2ＭＳ(大 ) ＮＡＡ 0 .0…