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Substitution of basic amino acids in the basic region stabilizes DNA binding by E12 homodimers. 总被引:1,自引:0,他引:1 下载免费PDF全文
The E2A gene encodes two alternatively spliced products, E12 and E47. The two proteins differ in their basic helix-loop-helix motifs (bHLH), responsible for DNA binding and dimerization. Although both E12 and E47 can bind to DNA as heterodimers with tissue-specific bHLH proteins, E12 binds to DNA poorly as homodimers. An inhibitory domain in E12 has previously been found to prevent E12 homodimers from binding to DNA. By measuring the dissociation rates using filter binding and electrophoretic mobility shift assays, we have shown here that the inhibitory domain interferes with DNA binding by destabilizing the DNA-protein complexes. Furthermore, we have demonstrated that substitution of basic amino acids (not other amino acids) in the DNA-binding domain of E12 can increase the intrinsic DNA-binding activity of E12 and stabilize the binding complexes, thus alleviating the repression from the inhibitory domain. This ability of basic amino acids to stabilize DNA-binding complexes may be of biological significance in the case of myogenic bHLH proteins, which all possess two more basic amino acids in their DNA binding domain than E12. To function as heterodimers with E12, the myogenic bHLH proteins may need stronger DNA binding domains. 相似文献
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Kikuchi Y Ohsawa S Mimura J Ema M Takasaki C Sogawa K Fujii-Kuriyama Y 《Journal of biochemistry》2003,134(1):83-90
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Cluster analysis of amino acid indices for prediction of protein structure and function 总被引:6,自引:0,他引:6
The relationship among 222 published indices representing various physicochemical and biochemical properties of amino acid residues has been investigated by hierarchical cluster analysis. The clustering result is illustrated by the minimum spanning tree, which is conveniently divided into four regions: alpha and turn propensities, beta propensity, hydrophobicity and other physicochemical properties including, among others, bulkiness of amino acid residues. In addition, several subclasses of hydrophobicity scales have been identified: preference of inside and outside, accessible surface area, surrounding hydrophobicity and other mostly experimental scales including transfer free energy, partition coefficients, HPLC parameters and polarity. Representative amino acid indices are identified in each of these groups. The collection of amino acid indices is a useful resource for empirical analyses correlating sequence information with structural and functional properties of proteins. As an example, the indices that best reproduce the amino acid mutation data matrix are searched against this collection. 相似文献
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Preferred sequences for DNA recognition by the TAL1 helix-loop-helix proteins. 总被引:22,自引:10,他引:12 下载免费PDF全文
H L Hsu L Huang J T Tsan W Funk W E Wright J S Hu R E Kingston R Baer 《Molecular and cellular biology》1994,14(2):1256-1265
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A leucine zipper protein of mitochondrial origin 总被引:1,自引:0,他引:1
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