共查询到20条相似文献,搜索用时 31 毫秒
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Inhibition of cellular proliferation by the Wilms' tumor suppressor WT1 is associated with suppression of insulin-like growth factor I receptor gene expression. 总被引:4,自引:2,他引:2
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H Werner Z Shen-Orr F J Rauscher rd J F Morris C T Roberts Jr D LeRoith 《Molecular and cellular biology》1995,15(7):3516-3522
We have investigated the regulation of the insulin-like growth factor I receptor (IGF-I-R) gene promoter by the Wilms' tumor suppressor WT1 in intact cells. The levels of endogenous IGF-I-R mRNA and the activity of IGF-I-R gene promoter fragments in luciferase reporter constructs were found to be significantly higher in G401 cells (a Wilms' tumor-derived cell line lacking detectable WT1 mRNA) than in 293 cells (a human embryonic kidney cell line which expresses significant levels of WT1 mRNA). To study whether WT1 could suppress the expression of the endogenous IGF-I-R gene, WT1-negative G401 cells were stably transfected with a WT1 expression vector. Expression of WT1 mRNA in G401 cells resulted in a significant decrease in the rate of cellular proliferation, which was associated with a reduction in the levels of IGF-I-R mRNA, promoter activity, and ligand binding and with a reduction in IGF-I-stimulated cellular proliferation, thymidine incorporation, and anchorage-independent growth. These data suggest that a major aspect of the action of the WT1 tumor suppressor is the repression of IGF-I-R gene expression. 相似文献
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WT1 suppresses synthesis of the epidermal growth factor receptor and induces apoptosis. 总被引:26,自引:2,他引:24
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C Englert X Hou S Maheswaran P Bennett C Ngwu G G Re A J Garvin M R Rosner D A Haber 《The EMBO journal》1995,14(19):4662-4675
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Cyclin E is a target of WT1 transcriptional repression 总被引:5,自引:0,他引:5
Loeb DM Korz D Katsnelson M Burwell EA Friedman AD Sukumar S 《The Journal of biological chemistry》2002,277(22):19627-19632
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WT1 modulates apoptosis by transcriptionally upregulating the bcl-2 proto-oncogene. 总被引:16,自引:0,他引:16
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M W Mayo C Y Wang S S Drouin L V Madrid A F Marshall J C Reed B E Weissman A S Baldwin 《The EMBO journal》1999,18(14):3990-4003
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Kanato K Hosen N Yanagihara M Nakagata N Shirakata T Nakazawa T Nishida S Tsuboi A Kawakami M Masuda T Oka Y Oji Y Ijpenberg A Hastie ND Sugiyama H 《Biochemical and biophysical research communications》2005,326(4):836-843
It is well known that the Wilms' tumor gene WT1 plays an important role in cell proliferation and differentiation, and in organ development. In this study, to examine the role of the WT1 gene in lineage determination, fetal liver cells from LacZ-transgenic mice, in which WT1 expression was marked by the expression of the LacZ gene driven by WT1 promoter, were FACS-sorted according to LacZ expression of high (LacZ(++)) or undetectable (LacZ(-)) levels, which paralleled endogenous WT1 expression levels. LacZ(++) fetal liver cells were enriched by hepatocyte and endothelial progenitor cells. These results indicated that WT1 expression is a common marker of both hepatocyte and endothelial progenitors. These results also implied a role of the WT1 gene in lineage determination. 相似文献
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The Wilms' tumor gene product, WT1, represses transcription of the platelet-derived growth factor A-chain gene. 总被引:16,自引:0,他引:16
Z Y Wang S L Madden T F Deuel F J Rauscher 《The Journal of biological chemistry》1992,267(31):21999-22002