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1.
In social insects, cuticular hydrocarbons of adults may vary with ovarian activity. Such variations are suggested to function in the regulation of reproduction within colonies. The modification of the CH profile with ovarian activity is usually interpreted as a signal of fertility causing workers to refrain from reproducing in the presence of the queen. We examined the effect of ovarian activity on the CH profiles in the Argentine ant Linepithema humile, a species where workers lack ovaries and are completely sterile. Our data show considerable differences in the CH profiles between fertile and infertile individuals. These differences are mainly qualitative. The CH profile strongly changes at the start of egg laying, both in mated and unmated queens. These results show that variation in cuticular hydrocarbons with ovarian activity in ants is not restricted to species with worker reproduction. We propose that in the Argentine ant, the cuticular hydrocarbons of laying queens correspond to a signal of fertility involved in the regulation of various aspects of reproduction, such as the rearing of new sexuals. 相似文献
2.
In a number of wasps, bees, and in particular ponerine ants, quantitative and qualitative variation in the profile of cuticular hydrocarbons is associated with variation in fecundity and is likely to serve for communicating the reproductive status of an individual. Here, we demonstrate that the chemical profile on the cuticle of fertile workers and queens of the myrmicine ant Temnothorax unifasciatus is different from that of non-reproductive workers. Fertility and apparently also cuticular signatures are reversible under the influence of policing by worker aggression. Though no policing by egg-eating occurs in this species, queen and worker laid eggs also differed in their chemical profile. 相似文献
3.
We analysed changes in cuticular hydrocarbon signatures of workers in orphaned colonies of the paper wasp Polistes dominulus. In natural conditions, workers and foundresses possess characteristic cuticular signatures, and foundresses are further distinguishable, both behaviourally and chemically, on the basis of their rank in a reproductive dominance hierarchy. In our study, several workers were found to develop their ovaries and produce cuticular signatures resembling those of dominant foundresses, while remaining workers possessed undeveloped ovaries and had cuticular blends characteristic of subordinate foundresses. Workers that did not develop their ovaries had changed epicuticular signatures, demonstrating that the mixture of hydrocarbons of worker individuals is strongly dependent on social role and environment. Our results suggest that the composition of epicuticular lipids is not determined at the pre-imaginal stage, and that physiological pathways leading to cuticular chemical changes are similar in foundresses and workers of P. dominulus. 相似文献
4.
Hamilton's theory [1] for the evolution of social behaviour predicts that helpers may renounce direct reproduction to help their more fertile kin. Intra-colony recognition among queens and helpers (subordinate queens or workers) is consequently a central issue in insect sociobiology. In social insects, cuticular hydrocarbons (CHCs) are involved in recognition, and egg-laying and non-egg-laying individuals often differ in CHC composition. These differences are assumed to be directly determined by fertility status [2,3]. In several ants and in Polistes wasps, when egg-layers disappear they are substituted by helpers, which develop their ovaries and become chemically similar to their former queens [2,3]. Sometimes helpers lay eggs in the presence of queens, which recognize and destroy the subordinates' eggs [4]. In ponerine ants, eggs often have the same chemical signature as the maternal cuticle [2]. If chemical signatures depend on fertility, egg-laying subordinates should match the queen's signature even when she is present, making egg recognition and differential oophagy impossible. In the study reported here, we experimentally separated fertility from dominance and analyzed the dynamics of hydrocarbon profiles of the cuticle of Polistes dominulus foundresses and the shell surface of their eggs. We have demonstrated that, contrary to the widely accepted view, dominance, rather than fertility, determines chemical signatures in Polistes wasps. This explains why queens can recognize their own eggs and police reproduction by subordinates if they become fertile and lay eggs. 相似文献
5.
Cuvillier-Hot V Gadagkar R Peeters C Cobb M 《Proceedings. Biological sciences / The Royal Society》2002,269(1497):1295-1300
In the monogynous queenless ant Diacamma ceylonense, the future reproductive (future gamergate) is very aggressive towards infertile workers during the first days of her adult life. Overt aggression disappears at about three weeks, when the future gamergate begins to lay male-destined eggs and is ready to mate. Over the same period, her cuticular hydrocarbon profile alters, changing from a chemical signature similar to that of a sterile worker towards that of a gamergate. In nature, these behavioural and chemical changes will coincide with a reduction in conflict within the nest: faced with a virgin future gamergate, infertile workers have an interest in producing male-destined eggs; however, once the gamergate produces female eggs, they have an interest in rearing her offspring. This demonstration of a shift from physical inhibition to chemical signalling is interpreted in terms of sociogenetic theory, the role of cuticular hydrocarbons as an indicator of fertility in insects and the fact that the regulation of reproduction in Diacamma involves mechanisms redolent of both queenless and queenright ant species. 相似文献
6.
In multiple-foundress nests of the wasp Polistes dominulus, dominance hierarchies are established among foundresses, and only the dominant (=alpha) individual lays eggs. The alpha female can be distinguished from subordinate females and workers on the basis of the proportions of some hydrocarbons present on the cuticle, suggesting that chemical signaling of her reproductive status could occur. P. dominulus is also the host species of the obligate social parasite Polistes sulcifer. After aggressively usurping host colonies and behaviorally replacing the host alpha female, parasites are characterized by a change in the proportions of their cuticular hydrocarbons to match that of the host cuticular profile at both species and colony levels. In the current study, we demonstrate that P. sulcifer queens also modify their cuticular hydrocarbon proportions after usurpation to match that of the host alpha female. Parasite females, therefore, acquire the dominant rank in host colonies both reproductively and chemically by mimicking the typical alpha profile of the host. Parasite females were not able to fully inhibit ovary development in host foundresses, and 10 days after usurpation, parasites, alpha and beta foundresses show similar chemical profiles and ovarian development. 相似文献
7.
Abdalla FC Jones GR Morgan ED da Cruz-Landim C 《Genetics and molecular research : GMR》2003,2(2):191-199
In social insects, cuticular hydrocarbons are involved in species, kin, caste and nestmate recognition. Gas chromatography and mass spectrometry were used to compare the cuticular hydrocarbon composition of workers, males and queens of Melipona bicolor. The cuticular hydrocarbon composition of this species was found to consist mainly of C23, C25:1, C25, C27:1, C27, C29:1 and C29, which are already present in imagoes that have not yet abandoned the brood cell. This composition varied quantitatively and qualitatively between and within the castes and sexes. The newly emerged workers and young queens (virgins) had similar cuticular hydrocarbon profiles, which were different from those of the males. When the females start executing their tasks in the colony, the cuticular hydrocarbon profile differences appear. The workers have less variety, while the queens conserve or increase the number of cuticular hydrocarbon compounds. The queens have more abdominal tegumentary glands than the workers, which apparently are the source of the new cuticular compounds. 相似文献
8.
In the queenless ant, Diacamma ceylonense, the cuticular hydrocarbons (C25-C35) of nestmate workers vary in their proportions according to age and fertility. Newly eclosed adults ('callows') initially have the same cuticular profile, but with time this changes to that typical of foragers. In contrast, workers that begin to produce eggs develop a different cuticular profile. Several substances (n-C29 and some methyl C25 and C27) discriminate these different social categories (callows, foragers and egg-layers). In Diacamma ceylonense, inter-colony variation of the cuticular hydrocarbons was much lower than intra-colony variation. We also found qualitative differences between the sexes, with males having a clearly different profile with much more alkanes. We discuss these results in the context of physiological models of the relation between ovarian activity and the synthesis of cuticular hydrocarbons. Variations in cuticular profile are a reliable reflection of ovarian activity, and could be used by ants as a fertility signal. 相似文献
9.
Eusocial insects exhibit reproductive division of labour, in which one or a few queens perform almost all of the reproduction, while the workers are largely sterile and assist in rearing their siblings. Consequently, many of the colony’s tasks (e.g. nest construction and brood rearing) should be modulated depending on whether the queen is fertile. Here, we tested whether queens’ cuticular lipids could provide reliable signals of fertility in the honeybee Apis mellifera, as they do in other social Hymenoptera. Specifically, we tested whether cuticular lipids differ between virgin queens of different ages, and between queens exposed to different artificial insemination treatments being semen (sperm and seminal fluid), seminal fluid only or saline control. Using gas chromatography–mass spectrometry, we found 27 lipids: 21 different hydrocarbons, namely alkanes, alkenes and dienes, as well as six wax esters. The lipid profile changed dramatically in the first 10 days after eclosion, and there were differences in lipid composition between virgin and artificially inseminated queens. Insemination with semen, seminal fluid or saline did not result in distinct chemical profiles. Our findings indicate that the physical stimulus of insemination was responsible for the observed changes in the cuticular profile in honeybee queens. Our results demonstrate that cuticular lipid profiles encode information on queen age, fertility and mating status, which could in principle be utilised by workers and rival queens. 相似文献
10.
Several studies have shown that differences in the relative abundance of cuticular hydrocarbons occur between reproductives and non-reproductives in many social insects. These differences also exist between Polistes dominulus foundresses and their first emerged daughters (usually indicated as workers), but they gradually disappear when workers from orphaned colonies develop their ovaries and assume a reproductive role in the colony. However, hydrocarbons are not the exclusive components of cuticular layer of wasps. Mass-spectrometry analysis of cuticular methanol extracts from Polistes paper wasps showed a complex pattern of polar substances, partly or totally proteinaceous in nature. We found that these compounds, ranging from 918 to 2679 Da, showed a clear caste differentiation between foundresses and their first emerged daughters (usually indicated as "workers"), both in queen-right and in orphaned colonies. Conversely to hydrocarbons, workers from orphaned colonies maintain a significant difference from foundresses in the pattern of the medium molecular weight (MW) polar compounds obtained by MALDI-TOF. On the basis of such results we hypothesize that a reliable cue to identify foundresses from daughters, and not only their reproductive status, may exist in Polistes wasps. Although the great majority of previous work on social insect communication has focused on cuticular hydrocarbons, our findings suggest that the medium MW component of cuticular substances may be involved in recognition. 相似文献
11.
J. Liebig C. Peeters B. H lldobler 《Proceedings. Biological sciences / The Royal Society》1999,266(1431):1865
Reproductive division of labour is an essential feature of insect sociality, but the regulation of sterility among colony members remains incompletely understood. Ant workers and queens are morphologically divergent and workers are only capable of producing males in a colony, although they usually do not do so. Worker policing is one mechanism proposed for their infertility and it can be expressed as either aggressive inhibition of ovarian activity among workers or destruction of worker-laid eggs. A few studies have shown that workers with developed ovaries are preferentially attacked by nest-mates, but adequate demonstration of worker policing also requires evidence that these attacks result in the suppression of ovarian activity or death. We investigated worker policing in the ponerine ant Harpegnathos saltator in which workers are able to mate and replace the founding queen. Five colonies were each divided into two groups, one of which consisted exclusively of infertile workers. Some individuals in the orphaned groups began laying eggs during the three-week separation and upon reunification these were vigorously attacked by infertile workers of the other groups. The ovarian activity of these new egg layers became inhibited, as revealed by subsequent dissection of marked individuals. Worker policing in H. saltator appears to function primarily in preventing an excess of reproductive workers. 相似文献
12.
Luke Holman Charlotte G. J?rgensen John Nielsen Patrizia d'Ettorre 《Proceedings. Biological sciences / The Royal Society》2010,277(1701):3793-3800
The selective forces that shape and maintain eusocial societies are an enduring puzzle in evolutionary biology. Ordinarily sterile workers can usually reproduce given the right conditions, so the factors regulating reproductive division of labour may provide insight into why eusociality has persisted over evolutionary time. Queen-produced pheromones that affect worker reproduction have been implicated in diverse taxa, including ants, termites, wasps and possibly mole rats, but to date have only been definitively identified in the honeybee. Using the black garden ant Lasius niger, we isolate the first sterility-regulating ant queen pheromone. The pheromone is a cuticular hydrocarbon that comprises the majority of the chemical profile of queens and their eggs, and also affects worker behaviour, by reducing aggression towards objects bearing the pheromone. We further show that the pheromone elicits a strong response in worker antennae and that its production by queens is selectively reduced following an immune challenge. These results suggest that the pheromone has a central role in colony organization and support the hypothesis that worker sterility represents altruistic self-restraint in response to an honest quality signal. 相似文献
13.
Role of hydrocarbons in egg recognition in the honeybee 总被引:3,自引:0,他引:3
Stephen J. Martin Graeme R. Jones Nicolas Châline Francis L. W. Ratnieks 《Physiological Entomology》2004,29(4):395-399
Abstract. Despite worker‐laid and queen‐laid honeybee eggs having significantly different hydrocarbon profiles, bioassays and chemical supplementation studies show that changing the hydrocarbon profile does not affect egg identity. Furthermore, full‐sized eggs that are tested just before being laid or just after being laid have similar hydrocarbon patterns but are treated differently in egg‐discrimination bioassays with only the laid eggs surviving. This suggests that hydrocarbons play no role in the egg‐recognition system in honeybees. 相似文献
14.
A key characteristic of eusocial species is reproductive division of labour. Honey bee colonies typically have a single reproductive queen and thousands of sterile workers. Adult queens differ dramatically from workers in anatomy, physiology, behaviour and lifespan. Young female workers can activate their ovaries and initiate egg laying; these 'reproductive' workers differ from sterile workers in anatomy, physiology, and behaviour. These differences, however, are on a much smaller scale than those observed between the queen and worker castes. Here, we use microarrays to monitor expression patterns of several thousand genes in the brains of same-aged virgin queens, sterile workers, and reproductive workers. We found large differences in expression between queens and both worker groups (~2000 genes), and much smaller differences between sterile and reproductive workers (221 genes). The expression patterns of these 221 genes in reproductive workers are more queen-like, and may represent a core group of genes associated with reproductive physiology. Furthermore, queens and reproductive workers preferentially up-regulate genes associated with the nurse bee behavioural state, which supports the hypothesis of an evolutionary link between worker division of labour and molecular pathways related to reproduction. Finally, several functional groups of genes associated with longevity in other species are significantly up-regulated in queens. Identifying the genes that underlie the differences between queens, sterile workers, and reproductive workers will allow us to begin to characterize the molecular mechanisms underlying the evolution of social behaviour and large-scale remodelling of gene networks associated with polyphenisms. 相似文献
15.
Since workers of the ant Aphaenogaster senilis can lay male eggs, reproductive conflict may occur between these workers. We examined the occurrence of worker conflicts
in groups of workers either with or without the queen. Intranidal aggression was observed in each nest for 10 min each day,
and the immatures produced were counted once a week for two months. Pairs of workers involved in aggression were taken regularly
from each nest and used for chemical, morphological and anatomical analyses. The attacker and the attacked workers differed
in their cuticular hydrocarbon profiles. The attacker and the attacked ants were at the same middle-aged fertile stage. The
attacker ant was significantly larger and more fertile than the attacked ant, and more mature physiologically (poison gland
was darker). There was apparently no stable hierarchy between laying workers. In the first weeks under queenless conditions,
most eggs and larvae were destroyed, but they were later reared to obtain males. The intranidal worker aggression in this
highly evolved ant is discussed in relation to dominance and worker policing.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
16.
Many colonies of the North American ant Crematogaster smithi contain a “third female caste” in addition to queens and workers. These “intermorphs” are morphological intermediate of queens and workers and have well-developed ovaries but lack a spermatheca for the storage of sperm. They are specialised for laying large numbers of unfertilised, viable eggs, most of which serve as food for larvae and adults, though a few may eventually develop into males. Based on the assumption that cuticular hydrocarbons (CHCs) in social insects honestly signal the reproductive status of an individual we investigated the CHC of mated mature queens, virgin queens, intermorphs and workers. We expected intermorphs to show chemical profiles intermediate between those of mated queens and non-reproductive workers. A discriminant analysis of the chemical profiles reliably separated queens, virgin queens, and workers, but failed to distinguish between queens and intermorphs even though workers were apparently capable of doing so. 相似文献
17.
Harald Jungnickel Hayo H. W. Velthuis†‡ Vera L. Imperatriz Fonseca¶ E. David Morgan 《Physiological Entomology》2001,26(4):300-305
Abstract. Queen-laid eggs of Melipona bees stand upright on their larval food, in part because the upper part of the egg is covered with a water-repellent layer of fatty acids and C21 to C29 hydrocarbons. The lower, wettable portion does not have these materials. Trophic eggs laid by workers are essentially devoid of this coating and tend to sink in the food, when not detected and consumed by the queen. Reproductive workers' eggs, which have about 50% of the water-repellent coating in comparison with the queen's eggs, are usually laid in the cell after oviposition by the queen, and therefore there are two eggs in the cell. Surface tension forces cause the two eggs to float towards each other so they become attached. When alone inside the cell, the reproductive worker egg often floats at an angle to the surface, reflecting the instability of this kind of egg. Attachment to a queen's egg therefore compensates this instability and enhances the reproductive worker egg's chances to develop. Such reproductive worker eggs have about 50% of the water-repellent coating in comparison with the queen's eggs. The surface of an 'undetermined' egg laid by a worker was much closer in composition to that of a queen's egg, from which it is deduced that this was the rarer type of reproductive worker egg, namely those that are laid before queen oviposition has taken place. The layer of hydrophobic material on a queen's egg is about one molecule thick, and probably orientated with the carboxyl groups toward the chorion of the egg and the hydrocarbon chains perpendicular to the surface. 相似文献
18.
Etya Amsalem Margarita Orlova Christina M. Grozinger 《Proceedings. Biological sciences / The Royal Society》2015,282(1817)
The regulation of reproductive division of labour is a key component in the evolution of social insects. Chemical signals are important mechanisms to regulate worker reproduction, either as queen-produced pheromones that coercively inhibit worker reproduction or as queen signals that honestly advertise her fecundity. A recent study suggested that a conserved class of hydrocarbons serve as queen pheromones across three independent origins of eusociality. In bumblebees (Bombus terrestris), pentacosane (C25) was suggested to serve as a queen pheromone. Here, we repeat these studies using a different species of bumblebee (Bombus impatiens) with a more controlled experimental design. Instead of dequeened colonies, we used same-aged, three-worker queenless groups comprising either experienced or naive workers (with/without adult exposure to queen pheromone). We quantified three hydrocarbons (C23, C25 and C27) on the cuticular surfaces of females and tested their effects on the two worker types. Our results indicate differences in responses of naive and experienced workers, genetic effects on worker reproduction, and general effects of hydrocarbons and duration of egg laying on ovary resorption rates. However, we found no evidence to support the theory that a conserved class of hydrocarbons serve as queen pheromones or queen signals in Bombus impatiens. 相似文献
19.
Our aim was to test the existence of Gestalt colony odour in Camponotus fellah. We isolated individual workers to prevent trophallaxis, allogrooming and body contact. After 20 days, the cuticular hydrocarbon profile of the isolated ants diverged from that of the parent colony. Moreover, each isolated individual had its own specific blend. This procedure showed that after about 20 days of isolation there was a turnover of the colony odour, revealing the genetically expressed hydrocarbon profile of each individual. It also showed that the cuticular hydrocarbon profile is polymorphic, and that its homogeneity within a colony is maintained by frequent exchanges of hydrocarbons between workers. Behavioural observations of resident workers, in their nest, towards nestmates reintroduced after isolation indicated that a short isolation period (3-5 days), which induced a minor change in hydrocarbon profile, provoked frequent trophallactic solicitations. These were likely to permit the isolated ants to readjust their hydrocarbon profile to that of the ants in the mother colony. Longer isolation periods (20-40 days) induced a greater change in hydrocarbon profile and made the residents intolerant towards their introduced nestmates. Therefore, our results clearly support the existence of a Gestalt colony odour in C. fellah. They also show that since individual hydrocarbon production is dynamic, workers are obliged to exchange hydrocarbons continually (mainly by trophallaxis) in order to be in the Gestalt, and properly integrate into the colony. Copyright 2000 The Association for the Study of Animal Behaviour. 相似文献
20.
D. KOEDAM P. H. VELTHAUSZ T. v. d. KRIFT M. R. DOHMEN M. J. SOMMEIJER 《Physiological Entomology》1996,21(4):289-296
Abstract. To study the reproductive potential of workers in the stingless bee Trigona (Tetragonisca) angustula Illiger (Apidae, Meliponinae), we examined the morphological quality of their eggs. Worker-eggs were all placed on the inside of the upper wall of brood cells. Normally, such eggs are consumed by the queen and are therefore referred to as trophic. The provisioning and oviposition processes in queenright colonies are characterized by the occurrence of circular aggregations of workers, 'rosettes', around the broodcell opening immediately after the release of a worker-egg. The ovaries of 35% of these rosette workers contained mature, chorionated eggs. In a single worker ovary, always only one mature egg was found. Some of the eggs, dissected from the ovarioles, showed a reticulate chorion pattern. Worker-eggs which lacked this pattern were significantly bigger than patterned eggs. Scanning electron micrographs revealed that the patterned worker-eggs are similar in appearance to queen-eggs.
After a worker-egg had been removed experimentally from a broodcell, the same cell could be oviposited by a worker again. Light microscopic analysis revealed that all these worker-eggs lacked the reticulate chorion pattern and were very similar in their morphology. In a colony without a laying queen workers laid eggs which had a reticulate pattern on the chorion. Since these eggs developed into males, we assume that the pattern on the chorion is characteristic for reproductive eggs. We also assume that the queen prevents the release of reproductive eggs by the workers. However, she does not inhibit the development of this type of egg. 相似文献
After a worker-egg had been removed experimentally from a broodcell, the same cell could be oviposited by a worker again. Light microscopic analysis revealed that all these worker-eggs lacked the reticulate chorion pattern and were very similar in their morphology. In a colony without a laying queen workers laid eggs which had a reticulate pattern on the chorion. Since these eggs developed into males, we assume that the pattern on the chorion is characteristic for reproductive eggs. We also assume that the queen prevents the release of reproductive eggs by the workers. However, she does not inhibit the development of this type of egg. 相似文献