IFT-A structure reveals carriages for membrane protein transport into cilia

1. Download : Download high-res image (389KB)
2. Download : Download full-size image

     

Enhanced validation of small-molecule ligands and carbohydrates in the Protein Data Bank

1. Download : Download high-res image (176KB)
2. Download : Download full-size image

     

Localized Inhibition of Protein Phosphatase 1 by NUAK1 Promotes Spliceosome Activity and Reveals a MYC-Sensitive Feedback Control of Transcription

1. Download : Download high-res image (184KB)
2. Download : Download full-size image

     

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis

1. Download : Download high-res image (109KB)
2. Download : Download full-size image

     

Revealing protein-protein interactions at the transcriptome scale by sequencing

1. Download : Download high-res image (123KB)
2. Download : Download full-size image

     

Molecular basis for recognition of Gly/N-degrons by CRL2ZYG11B and CRL2ZER1

1. Download : Download high-res image (104KB)
2. Download : Download full-size image

     

PDB-wide identification of physiological hetero-oligomeric assemblies based on conserved quaternary structure geometry

1. Download : Download high-res image (339KB)
2. Download : Download full-size image

     

Ubiquitin-independent proteasomal degradation driven by C-degron pathways

1. Download : Download high-res image (177KB)
2. Download : Download full-size image

     

Epigenetic reader SP140 loss of function drives Crohn’s disease due to uncontrolled macrophage topoisomerases

1. Download : Download high-res image (174KB)
2. Download : Download full-size image

     

mTORC1 promotes cell growth via m6A-dependent mRNA degradation

1. Download : Download high-res image (124KB)
2. Download : Download full-size image

     

Assessing PDB macromolecular crystal structure confidence at the individual amino acid residue level

1. Download : Download high-res image (120KB)
2. Download : Download full-size image

     

Simplified quality assessment for small-molecule ligands in the Protein Data Bank

1. Download : Download high-res image (137KB)
2. Download : Download full-size image

     

Open-access data: A cornerstone for artificial intelligence approaches to protein structure prediction

1. Download : Download high-res image (84KB)
2. Download : Download full-size image

     

A Cross-linking Mass Spectrometry Approach Defines Protein Interactions in Yeast Mitochondria

1. Download : Download high-res image (250KB)
2. Download : Download full-size image

Highlights

• •XL-MS reveals new PPIs in yeast mitochondria under glycerol and glucose condition.
• •Significant but limited results from quantitative XL-MS experiments.
• •Ndi1 participates in a CIII₂CIV₂ respiratory supercomplex.
• •Min8 promotes assembly of Cox12 into an intermediate complex IV.

     

TRIP12 ubiquitination of glucocerebrosidase contributes to neurodegeneration in Parkinson’s disease

1. Download : Download high-res image (255KB)
2. Download : Download full-size image

     

Developments and Applications of Functional Protein Microarrays

1. Download : Download high-res image (222KB)
2. Download : Download full-size image

Highlights

• •Summarize the development of functional protein microarray.
• •Application of functional proteome microarray in basic research.
• •Application of functional proteome microarray in translational research.
• •Fabrication of functional membrane protein array using virion display method.

     

MaXLinker: Proteome-wide Cross-link Identifications with High Specificity and Sensitivity

1. Download : Download high-res image (339KB)
2. Download : Download full-size image

Highlights

• •New quality assessment metrics to evaluate proteome-wide cross-linking mass spectrometry (XL-MS) data sets.
• •New “MS3-centric” cross-link search engine named MaXLinker with high sensitivity and specificity.
• •More than 9300 cross-links from our human proteome-wide XL-MS study.
• •Orthogonal experimental validation of novel interactions identified in our study.

     

The Capture of a Disabled Proteasome Identifies Erg25 as a Substrate for Endoplasmic Reticulum Associated Degradation

1. Download : Download high-res image (133KB)
2. Download : Download full-size image

Highlights

• •Epitope-tagging of a proteasome subunit allows for facile immuno-isolation.
• •An engineered yeast strain permits capture of proteasome-associated substrates.
• •MS/MS identified all 33 resident proteasome subunits in the 20S and 19S particles.
• •Analysis of associated proteins and characterization of newly identified ERAD substrate.

     

OpenPepXL: An Open-Source Tool for Sensitive Identification of Cross-Linked Peptides in XL-MS

1. Download : Download high-res image (245KB)
2. Download : Download full-size image

Highlights

• •OpenPepXL is a new XL-MS identification tool with a high sensitivity.
• •It is available for all common operating systems and remote computing environments.
• •OpenPepXL is open source and supports open OpenPepXL is available as part of OpenMS data formats like mzML and mzIdentML.
• •at https://www.openms.de/openpepxl.

     

Temporal Quantitative Proteomics of mGluR-induced Protein Translation and Phosphorylation in Neurons

1. Download : Download high-res image (183KB)
2. Download : Download full-size image

Highlights

• •Integrated phosphoproteomics and analyses of newly synthesized proteins in neurons.
• •Resource of temporal mGluR-induced signaling pathways upon DHPG stimulation.
• •Validation of PKC, MAPK1, CAMKIIa, and CDK2 in mGluR-activation and signaling.
• •Validation of Intersectin-1 in DHPG-induced AMPAR internalization.