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1.
Henri Faure Alain Favier Michele Tripier Josiane Arnaud 《Biological trace element research》1990,24(1):25-37
In this paper we report a method for measuring ultrafiltrable zinc in human serum by electrothermal atomic absorption spectrophtometry.
We show also that ultrafiltration permits to determine alpha-2 macroglobulin bound zinc and losely bound zinc if a strong
zinc ligand (EDTA) is added to serum before ultrafiltration. This last fraction, after deduction of ultrafiltrable zinc, represents
roughly all albumin bound zinc. In 20 controls we found that ultrafiltrable zinc amounted 0.311 μmol/L (S.D.=0.117 μmol/L),
alpha-2 macroglobulin bound zinc 3.08 μmol/L (S.D.=0.221 μmol/L), and albumin bound zinc 12.11 μmol/L (S.D.=1.95 μmol/L).
Our method needs only a small volume of serum, it is simple and rapid but also very accurate and reliable. The losely bound
fraction is very dynamic and, representing the physiologically active part of serum zinc, it could be a good marker of zinc
deficiency. 相似文献
2.
Jerome Honnorat Michele Accominotti Christiane Broussolle Andree-Carole Fleuret Jean-Jacques Vallon Jacques Orgiazzi 《Biological trace element research》1992,32(1-3):311-316
Zinc status was assessed in 53 diabetic patients: 18 insulin-dependent diabetic patients (IDDM), 22 noninsulin-dependent diabetic
patients (NIDDM) treated with oral antidiabetic agents, and 13 insulin-treated, noninsulin-dependent diabetic patients (IRDM).
Plasma zinc concentrations were in the usual range for healthy subjects in these three groups (15.3±0.9 μmol/L). Urinary zinc
excretions were elevated in the IDDM group (18.3±4.1 μmol/24 h;p<0.01 vs normal) and in the NIDDM group (17.5±3.5 μmol/24 h;p<0.01 vs normal), but normal in the IRDM group (11.3±2.4 μmol/24 h). In 14 NIDDM patients treated with transient continuous
sc insulin injections, urinary zinc decreased from 16.5±2.2 μmol/24 h before insulin treatment to 11.5±0.3 μmol/24 h after
insulin treatment without any modification in plasma zinc concentrations. 相似文献
3.
The concentrations of zinc and copper in gastric juice of humans who had widely varying dietary zinc intake were evaluated.
In order to compare this with zinc and copper levels of normal dietary individuals, we also determined the zinc and copper
levels in healthy individuals' plasma and in cancer patient's natural tissue, all of whom had normal diets. The correlation
coefficients between zinc and copper were 0.71, 0.45, and 0.55, respectively, in gastric juice, plasma, and tissue of normal
dietary subjects. Such correlation changed and was destroyed when there was a high zinc level in gastric juice. When gastric
juice zinc level changed from mean value 16.8 μmol/L to 262.5 μmol/L, the correlation coefficient varied from 0.71 to −0.04,
and the copper level also varied from mean value 8.96 μmol/L to 4.89 μmol/L. These findings probably give the evidence to
suggest that a high zinc level will restrain the copper level and break the balance of the human body's zinc and copper metabolism. 相似文献
4.
Estelle Thauvin Michelle Fusselier Josiane Arnaud Henri Faure Max Favier Charles Coudray Marie-Jeanne Richard Alain Favier 《Biological trace element research》1992,32(1-3):405-414
The effect of a multivitamin-mineral supplement was investigated during pregnancy according to a double-blind protocol by
determining zinc and copper in maternal plasma, mononuclear and polynuclear zinc and copper at the third, sixth, eighth, and
ninth months of gestation. The subjects were supplemented from the first trimester until delivery. A significant decrease
was observed in plasma zinc that varied from 11.5 μmol/L to 10.8 μmol/L in the supplemented group (n=29) and from 11 μmol/L to 10 μmol/L in the placebo group (n=33) at 3 and 9 mo of gestation, respectively. In contrast, plasma copper levels increased in a way depending upon the stage
of gestation in both groups: from 24.7 to 28.2 μmol/L in the treated group and from 24.9 to 30.9 μmol/L in the placebo group
at 3 and 9 mo of gestation, respectively, but the difference was only significant in the placebo group. No difference between
groups was observed in mononuclear and polynuclear zinc or copper levels.
These trace elements were also determined in cord blood at delivery. There were no statistically significant differences in
zinc and copper concentration found in placebo group and supplemented group. Finally, the beneficial effect of supplementation
on muscular cramps and appearance of vergetures was noted. 相似文献
5.
Marie J. Richard Veronique Ducros Michel Rorêt Josiane Arnaud Charles Coudray Michèle Fusselier Alain Favier 《Biological trace element research》1993,39(2-3):149-159
In six chronic dialyzed uremic patients, an intravenous sodium selenite (Se 50 μg during 5 wk and then 100 μg) and zinc gluconate
(Zn 5 mg) supplementation was performed during 20 wk at each dialysis session three times weekly. Before supplementation,
plasma Se and Zn, plasma and erythrocytes (RBC) antioxidant metalloenzymes glutathione peroxidase (GPX), and superoxide dismutase
(SOD) were significantly decreased, whereas lipid peroxidation (as thiobarbituric acid reactants TBARs) was increased. To
obtain a significative change in plasma selenium, we had to use an Se dose of 100 μg/dialysis session. Then, treatment-increased
plasma Se (from 0.58 ±0.09 to 0.89±0.16 μmol/L) led to a repletion of RBC-GPX (from 29.6±6 to 43±5.8 U/g Hb) and increased
plasma GPX levels (from 62±13 to 151±43 U/L). Plasma Zn and RBC-SOD did not vary significantly. The change of TBARs was not
observed between wk 1 and 4. They decreased significantly between wk 4 (4.80±0.21μmol/L) and wk 20 (4.16±0.26 μmol/L). We
noted a low correlation between TBARs and plasma GPX. A strong correlation was observed between Se and plasma GPX. The reversal
of Se deficiencies should reduce oxidative damage observed in these patients. 相似文献
6.
Riboflavin, suggested to be a radiosensitizer, was studied in murine thymocytes and human hepatoma L02 cell line in vitro with MTT method and fluorescence microscopy. When the murine thymocytes treated with 5–400 μmol/L riboflavin were irradiated
by 5 Gy 60Co γ ionizing radiation, the low concentration groups, i.e. treated with 5–50 μmol/L riboflavin, showed a different surviving
fractions-time relating correlation compared with the high concentration groups, i.e. treated with 100–400 μmol/L riboflavin.
The former had a high survival level at the end of irradiation, but which, after 4-h incubation, decreased rapidly to a low
level. On the contrary, the high concentration groups showed a low survival level at the end of irradiation, and a poor correlation
was found between the surviving fraction and the incubation time, after 4 h a little difference was observed. The results
of fluorescence microscopy indicated that under low concentration conditions, the riboflavin localized mainly in nucleus (both
perinuclear area and inside of nuclear membrane), while under high concentration conditions, intensive riboflavin also localized
around cytoplasmic membranes. Thus we can conclude: the riboflavin had radiosensitivity effect on DNA under low concentration
conditions, and enhanced the damage to cytoplasmic membrane under high concentration conditions. Also the most effective concentration
of riboflavin can be evaluated to be approximate 100 μmol/L. 相似文献
7.
The effects of selenium, zinc, iron, chromium, and lead on telomere lengths of human cells have not been investigated. This
article adopted flow cytometry and fluorescence in situ hybridization to investigate the impact of different elements on cellular apoptosis and telomere lengths of human hepatocytes
L-02 and hepatoma cells SMMC-7721. Results showed that these trace elements under the following dosages did not have remarkable
effect on cellular apoptosis. However, sodium selenite at doses of 0.5 and 2.5 μmol/L significantly extended the telomere
length of hepatocytes L-02; 0.5 μmol/L lead acetate remarkably shortened the telomere length of L-02 cells; 80 μmol/L zinc
sulfate, 20 μmol/L ferric chloride, and 200 μmol/L chromic chloride only had slight impact on the telomere length, respectively.
Regarding hepatoma cells SMMC-7721, sodium seleite at 0.5 and 2.5 μmol/L had little impact on the telomere length; 80 μmol/L
zinc sulfate significantly accelerated the loss of telomere length, whereas 20 μmol/L ferric chloride, 200 μmol/L chromic
chloride, and 0.5 μmol/L lead acetate remarkably extended the telomere lengths, respectively. The results revealed differential
effects of each trace element on the life-span of human hepatocytes and hepatoma cell lines, which suggested further research
on somatic hepatocytes and hepatoma in vivo. 相似文献
8.
Casimiro-Lopes G de Oliveira-Junior AV Portella ES Lisboa PC Donangelo CM de Moura EG Koury JC 《Biological trace element research》2009,127(2):109-115
The purpose of this study was to compare plasma leptin, plasma zinc, and plasma copper levels and their relationship in trained
female and male judo athletes (n = 10 women; n = 8 men). Blood samples were obtained 24 h after training to measure plasma zinc, copper, and leptin levels. Subjects presented
similar values to age (22 ± 2 years old), body mass index (24 ± 1 kg/m2), plasma zinc (17.2 ± 2 μmol/L), copper (12.5 ± 2 μmol/L), and leptin (5.6 ± 1.3 μg/L). However, height, total body mass,
lean mass, fat mass, and sum of ten-skinfold thickness were higher in male than female. Plasma leptin was associated with
sum of ten skinfolds in male (r = 0.91; p < 0.001) and female athletes (r = 0.84; p < 0.003). Plasma zinc was associated with leptin in males (r = 0.82; p < 0.05) while copper was associated with plasma leptin in females (r = 0.66; p < 0.05). Our results suggest that young judo athletes lost sex-related differences in leptin levels. Plasma zinc, plasma
copper, and energy homeostasis may be involved in regulation of plasma leptin. 相似文献
9.
Lecoeur S Huynh-Delerme C Blais A Duché A Tomé D Kolf-Clauw M 《Cell biology and toxicology》2002,18(6):409-423
The mechanisms of intestinal absorption have not been clearly elucidated for cadmium, a toxic metal. In this work, we show
the implication of distinct proteins in cadmium transport, and the transport step where these proteins are involved. We first
validated the HT-29 model by evaluating nontoxic doses of cadmium (ranging from 1 to 20 μmol/L), and by quantifying metal
uptake and transepithelial transport. The time-course of 1 μmol/L cadmium uptake at pH 7.5 showed three steps: a rapid one
during the first 4 min, probably due to cadmium binding to the membrane; a slower one, characterized by K
m of 1.65±0.54 μmol/L and V
max of 3.9±0.3 pmol/min per mg protein; and a third, corresponding to slow accumulation that was not equilibrated even after
48 h of cadmium exposure. Intracellular metallothionein content following 1 or 5 μmol/L cadmium exposure showed a significant
increase after 6 h of exposure, and was not equilibrated even after 72 h, allowing cadmium accumulation. After 24 h of exposure,
metallothionein content was 5-fold, 14-fold, 26-fold, and 50-fold, respectively, for cells grown in the presence of 1, 5,
10, and 20 μmol/L cadmium, compared to control cells. The second step of uptake, characterized by carrier-mediated transport,
was markedly increased at pH 5.5, compared to pH 7.5, and strongly inhibited by the metabolic inhibitor dinitrophenol. Moreover
Nramp2 transporter cDNA was present in HT-29 cells. These data suggest the involvement of a proton-coupled transporter, which
may be the divalent cation transporter Nramp2 (natural resistance-associated macrophage protein 2). Cadmium uptake was also
inhibited by copper, zinc, and para-chloromercuribenzenesulfonate (pCMBS), but not by verapamil or ouabain. Taken together, our results indicate that cadmium
could enter HT-29 cell by Nramp2 proton-coupled active transport and by diffusion, and accumulates in the cell as long as
it binds to metallothionein. Cadmium toxicity could depend partly on the activity of Nramp2, and partly on metallothionein
content.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
10.
Hee Y. Paik Hyojee Joung Joo Y. Lee Hong K. Lee Janet C. King Carl L. Keen 《Biological trace element research》1999,69(1):45-57
The present study focused on whether serum extracellular superoxide dimutase (EC-SOD) activity can be used as a functional
indicator of marginal zinc deficiency in humans. Subjects in this study were 444 healthy adults over 30 yr of age living a
normal rural life in Kyunggi province, Korea. The mean dietary zinc intake of subjects obtained from one 24-h recall was 6.41
± 4.35 mg and the average serum zinc concentration of the subjects was 11.06 ± 2.44 (μmol/L. Subjects were divided into three
groups by serum zinc concentrations: adequate (serum zinc >10.7 (μmol/L), low (serum zinc 9.0–10.7 μmol/L), and very low (serum
zinc <9.0 μmol/L) groups. A total of 50 subjects were selected from the three groups for analysis of EC-SOD activities. The
EC-SOD activity of subjects increased with increasing serum zinc concentrations, and the activities of the three groups were
significantly different as indicated by the Kruskal-Wallis test (p = 0.0239). Also, serum EC-SOD activities were significantly
correlated with serum zinc concentrations (r = 0.289,p = 0.04). Serum EC-SOD activities, however, were not significantly correlated to the dietary zinc intakes. In conclusion,
these results show that EC-SOD activities are decreased in subjects with low serum zinc concentrations and suggest that EC-SOD
activity may be a functional indicator of zinc nutritional status in humans. 相似文献
11.
Kedzierska K Bober J Ciechanowski K Gołembiewska E Kwiatkowska E Noceń I Dołegowska B Dutkiewicz G Chlubek D 《Biological trace element research》2005,107(1):21-32
The aim of the study was to verify the hypothesis if copper could influence the activity of sodium-transporting systems in
erythrocyte membrane that could be related to essential hypertension. The examined group of patients consisted of 15 men with
hypertension. The control group was 11 healthy male volunteers. The Na+/H+ exchanger (NHE) activity in erythrocytes was determined according to Orlov et al. The activity of transporting systems (ATP-Na+/K+; co-Na+/K+/Cl−; ex-Na+/Li+; free Na+ and K+ outflow [Na+, K+-outflow]) was determined according to Garay's method. The concentration of copper in plasma was assessed using atomic absorption
spectrometry. The activity of ATP-Na+/K+ (μmol/L red blood cells [RBCs]/h) in hypertensive patients was 2231.5±657.6 vs 1750.5±291 in the control (p<0.05), the activity of co-Na+/K+/Cl− (μmol/L RBCs/h) in hypertensives was 171.3±77.9 vs 150.7±53.9 in the control (NS). Na+-outflow (μmol/L RBCs/h) in hypertensives was 118.3±51.6 vs 113.3±24.4 in the control (NS). The K+-outflow (μmol/L RBCs/h) in hypertensives was 1361.7±545.4 vs 1035.6±188.3 in the control (NS). The activity of ex-Na+/Li+ (μmol/L RBCs/h) in hypertensive patients was 266.1±76.1 vs 204.1±71.6 in the control (p<0.05). NHE activity (mmol/L RBCs/h) in hypertensives was 9.7±2.96 vs 7.7±1.33 in the control (p<0.05). In hypertensive patients, negative correlation was found between the activity of Na+/K+/Cl− co-transport and plasma copper concentration (R
s=−0.579, p <0.05) and between the activity of ex-Na+/Li+ and plasma copper concentration (R
s=−0.508, p<0.05). Plasma copper concentration significantly influences the activity of sodium transporting systems in erythrocyte membrane.
Copper supplementation could be expected to provide therapeutic benefits for hypertensive patients. 相似文献
12.
Attila Vig Attila Igloi Nora Adanyi Gyongyi Gyemant Csaba Csutoras Attila Kiss 《Bioprocess and biosystems engineering》2010,33(8):947-952
An amperometric detector and an enzymatic reaction were combined for the measurement of l-ascorbic acid. The enzyme cell (containing immobilized ascorbate oxidase) was connected to a flow injection analyzer (FIA)
system with a glassy carbon electrode as an amperometric detector. During optimization and measurements two sample injectors
were used, one before and one after the enzyme cell, thus eliminating the background interferences. Subtraction of the signal
area given in the presence of enzyme from the one given in the absence of enzyme was applied for measuring analyte concentrations
and calibration at 400 mV. Analysis capacity of system is 25 samples/hour. The relative standard deviation (RSD) was below
5% (5 times repeated, 400 μmol/L conc.), linearity up to 400 μmol/L, limit of detection (LOD) 5 μmol/L, fitting of calibration
curve in 25–400 μmol/L range was R
2 = 0.99. 相似文献
13.
The effectiveness and success of antitubercular therapy is mainly measured by identifying the organism in sputum. In certain
patients, especially in geriatric patients, available tuberculosis tests are not satisfactory and do not provide enough information
on the effectiveness of antitubercular therapy, as the symptoms might be confused with the existing symptoms of ongoing diseases.
Therefore, 60 diagnosed and randomly selected patients with tuberculosis were included into this study. The patients with
other associated diseases likely to influence serum copper and zinc were not included in the study. The estimations of serum
copper and zinc were done in healthy volunteers and in tubercular patients before the start of treatment and after 4 wk of
antitubercular treatment. The average plasma concentration of serum copper and zinc in healthy volunteers were 102±20 μg/dL
and 96±18 μg/dL respectively. In tuberculosis patients, serum copper and zinc levels were 123.65±9.98 μg/dL and 64.14±3.97
μg/dL, respectively, before the start of treatment, which came down to 116.23±4.27 μg/dL and 74.31±3.60 μg/dL, respectively,
after 4 wk of antitubercular treatment. 相似文献
14.
Valsartan orodispersible tablets have been developed at 40-mg dose, with the intention of facilitating administration to patients
experiencing problems with swallowing and hopefully, improving its poor oral bioavailability. Work started with selecting
drug compatible excipients depending on differential scanning calorimetric analysis. A 33 full factorial design was adopted for the optimization of the tablets prepared by freeze-drying technique. The effects of
the filler type, the binder type, and the binder concentration were studied. The different tablet formulas were characterized
for their physical properties, weight variation, disintegration time, surface properties, wetting properties, and in vitro dissolution. Amongst the prepared 27 tablet formulas, formula number 6 (consisting of 4:6 valsartan:mannitol and 2% pectin)
was selected to be tested in vivo. Oral bioavailability of two 40 mg valsartan orodispersible tablets was compared to the conventional commercial tablets after
administration of a single dose to four healthy volunteers. Valsartan was monitored in plasma by high-performance liquid chromatography.
The apparent rate of absorption of valsartan from the prepared tablets (C
max = 2.879 μg/ml, t
max = 1.08 h) was significantly higher than that of the conventional tablets (C
max = 1.471 μg/ml, t
max = 2.17 h), P ≤ 0.05. The relative bioavailability calculated as the ratio of mean total area under the plasma concentration–time curve
for the orodispersible tablets relative to the conventional ones was 135%. The results of the in vivo study revealed that valsartan orodispersible tablets would be advantageous with regards to improved patient compliance, rapid
onset of action, and increase in bioavailability. 相似文献
15.
Cadmium-induced apoptosis of primary epithelial lung cells: Involvement of Bax and p53, but not of oxidative stress 总被引:11,自引:0,他引:11
Lag M Westly S Lerstad T Bjørnsrud C Refsnes M Schwarze PE 《Cell biology and toxicology》2002,18(1):29-42
The lung is a target organ for cadmium (Cd) toxicity. Apoptosis induced by cadmium acetate (CdAc) was studied in alveolar
type 2 cells and Clara cells isolated from rat lung. Relatively low concentrations of CdAc (1–10 μmol/L) induced apoptosis
after exposure for 20 h. Type 2 cells were more sensitive than Clara cells to Cd-induced apoptosis and loss of cell viability.
On exposure to 10 μmol/L CdAc, the levels of the apoptosis-modulating proteins p53 and Bax were increased at 2 h and 5–12
h, respectively. The expression of p53 preceded the expression of Bax and the apoptotic process. The exposure to 10 μmol/L
CdAc did not significantly increase the formation of cellular reactive oxygen species (ROS). However, after exposure to a
high concentration of CdAc (100 μmol/L), a 30% increase of the ROS level was observed. No significant nitric oxide production
was measured following CdAc exposure. Catalase, superoxide dismutase, dimethyl sulfoxide, or tetramethylthiourea did not protect
against Cd-induced apoptosis. In conclusion, the results show that Clara cells and type 2 cells are sensitive to Cd-induced
apoptosis. Increased levels of p53 and Bax are suggested to be involved in the apoptosis. The apoptosis did not appear to
be mediated by oxidative pathways.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
16.
Lead and catechol hematotoxicity in vitro using human and murine hematopoietic progenitor cells 总被引:1,自引:0,他引:1
In vitro cloning assays for hematopoietic myeloid and erythroid precursor cells have been used as screening systems to investigate
the hematotoxic potential of environmental chemicals in humans and mice. Granulocyte-monocyte progenitors (CFU-GM) from human
umbilical cord blood and from mouse bone marrow (Balb/c and B6C3F1) were cultured in the presence of lead and the benzene
metabolite catechol. Erythroid precursors (BFU-E) from human umbilical cord blood were cultured in the presence of lead. The
in vitro exposure of the human and murine cells resulted in a dose-dependent depression of the colony numbers. The concentration–effect
relationship was studied. Results showed that:
(1) Based on calculated IC50 values, human progenitors are more sensitive to lead and catechol than are murine progenitors.
The dose that caused a 50% decrease in colony formation after catechol exposure was 6 times higher for murine cells (IC50
= 24 μmol/L) than for human cord blood cells (IC50 = 4 μmol/L). Lead was 10–15 times more toxic to human hematopoietic cells
(IC50 = 61 μmol/L) than to murine bone marrow cells from both mice strains tested (Balb/c, IC50 = 1060 μmol/L; B6C3F1, IC50
= 536 μmol/L).
(2) A lineage specificity was observed after exposure to lead. Human erythroid progenitors (hBFU-E) (IC50 = 3.31 μmol/L) were
found to be 20 times more sensitive to the inhibitory effect of lead than were myeloid precursors (hCFU-GM) (IC50 = 63.58
μmol/L).
(3) Individual differences in the susceptibility to the harmful effect of lead were seen among cord blood samples.
(4) Toxicity of lead to progenitor cells occurred at environmentally relevant concentrations.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
17.
Marques RC de Sousa AF do Monte SJ Oliveira FE do Nascimento Nogueira N Marreiro DN 《Biological trace element research》2007,120(1-3):11-18
Studies have evidenced that zinc metabolism is altered in presence of Down syndrome, and zinc seems to have a relationship
with the metabolic alterations usually present in this syndrome. In this work, the Zn-related nutritional status of adolescents
with Down syndrome was evaluated by means of biochemical parameters and diet. A case–control study was performed in a group
of adolescents with Down syndrome (n = 30) and a control group (n = 32), of both sexes, aged 10 to 19 years. Diet evaluation was accomplished by using a 3-day dietary record, and the analysis
was performed by the NutWin program, version 1.5. Antropometric measurements were performed for evaluation of body composition.
The Zn-related nutritional status of the groups was evaluated by means of zinc concentration determinations in plasma and
erythrocytes, and 24-h urinary zinc excretion, by using the method of atomic absorption spectroscopy. The diet of both groups
presented adequate concentrations of lipids, proteins, carbohydrates, and zinc. The mean values found for zinc concentration
in erythrocytes were 49.2 ± 8.5 μg Zn/g Hb for the Down syndrome group and 35.9 ± 6.1 μg Zn/g Hb for the control group (p = 0.001). The average values found for zinc concentration in plasma were 67.6 ± 25.6 μg/dL for the Down syndrome group and
68.9 ± 22.3 μg/dL for the control group. The mean values found for zinc concentration in urine were 244.3 ± 194.9 μg Zn/24 h
for the Down syndrome group and 200.3 ± 236.4 μg Zn/24 h for the control group. Assessment of body composition revealed overweight
(26.7%) and obesity (6.6%) in the Down syndrome group. In this study, patients with Down syndrome presented altered zinc levels
for some cellular compartments, and the average zinc concentrations were low in plasma and urine and elevated in erythrocytes. 相似文献
18.
Abdellatif Chakar Ridha Mokni Philip A. Walravens Philippe Chappuis Fanny Bleiberg-Daniel Jean-Louis Mahu Daniel Lemonnier 《Biological trace element research》1993,38(1):97-106
Plasma zinc, copper, and parameters of growth were measured in a group of 116 French preschool children, 2–5 yr-old from low-income
households. Participants were selected on the basis of Z-scores of weight for height (WHZ) and height for age (HAZ). Zinc
and copper concentrations of children with growth impairment (GI), defined by a WHZ and/or HAZ< −1 Z-score, were compared
to those of age, sex, and ethnic origin matched controls (WHZ and HAZ >−1 Z-score). Mean (±SD) plasma zinc concentration was
12.58±1.84 μmol/L in the GI group, and 13.27±1.98 μmol/L in the controls. The difference of the means of paired samples was
0.69±2.34, and by pairedt-test the significance reachedp=0.028. This effect was primarily a result of the weight retarded group (WHZ <−1 Z-score,p<0.009) and to the girls (p<0.05). There were no significant differences in plasma copper concentrations between groups. These results suggest the presence
of marginal zinc deficiency in French preschool children with low weight for height Z-scores. 相似文献
19.
Zinc and copper have been estimated in CSF of 14 normal volunteers, nine men and five women. Zinc was analyzed by limited-aspiration
flame atomic absorption spectrophotometry using a deuterium continuum light source. Copper was analyzed in 0.1% HNO3 by flameless atomic absorption spectrophotometry with a graphite cuvette on a flameless atomizer. Recovery of added zinc
varied less than 5% and that of the added copper varied less than 8%. CSF zinc was 31.5±19.8 μg/L (mean ± 1 SD); CSF copper,
7.5±3.1 μ/L. Values obtained for CSF zinc are about 1/2 those we and others obtained previously, the decrease related almost
exclusively to removal of interference by the CSF matrix, which produced spuriously elevated values without use of the deuterium
light source. Values obtained for CSF copper were approximately one-tenth those we and others had obtained previously. The
decrease related, in part, to the removal of matrix effects, but also to improvement of the signal-to-noise ratio present
in other techniques. 相似文献
20.
Antti Aro Jorma Kumpulainen Georg Alfthan Anatoly V. Voshchenko Vladimir N. Ivanov 《Biological trace element research》1994,40(3):277-285
The selenium concentration in foods grown and consumed and in plasma, red blood cells, and toenails of people living in the
district of Chita in the transbaikalian part of Russia were studied in August 1991. Preliminary results from the area have
suggested low selenium intakes and the possible occurrence of cardiomyopathy (Keshan disease) in the population. A low selenium
concentration in foods grown locally was found: mean selenium concentration in wheat grains was 1, 5, and 28 μg/kg, respectively,
in three villages studied, that of oats was beween 3–6 μg/kg, and of cow's milk 10–27 μg/kg dry matter. The selenium concentration
of bread was considerably higher, between 87–337 μg/kg dry wt, presumably because wheat imported from the US had been used
for baking. Occasional samples of pork, beef, and mutton contained between 32–318 μg selenium/kg dry wt. Low selenium concentrations
were observed in samples of soil and river water. The mean plasma selenium concentration of 52 persons was 1.02 μmol/L, including
33 children and 19 adult subjects. The selenium concentrations in red blood cells and toenails were 1.95 μmol/L and 0.61 mg/kg,
respectively. No symptoms of heart disease caused by selenium deficiency were observed. It is concluded that the selenium
status of people was fairly good thanks to the contribution to dietary intake of imported wheat with a high selenium content.
As the selenium concentration was very low in foods grown in the area, the selenium intake of the population will be reduced
to a very low level if only locally produced foods are consumed. 相似文献