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1.
《Comparative biochemistry and physiology. A, Comparative physiology》1989,92(3):377-384
- 1.1. Cells of tentacles and body wall of the sea anemone Condylactis gigantea behaved as simple osmometers during 5hr exposure to 50, 67, 83, 100 and 125% sea-water.
- 2.2. All intracellular water appeared to be osmotically active.
- 3.3. Cell sodium, chloride and total osmolyte content remained invariable, with taurine decreasing and potassium increasing as sea-water concentration was reduced.
- 4.4. Tissues, as a whole, exhibited a pseudoregulatory response to changes in salinity as the large and osmotically inert extracellular space buffered volume changes to a considerable extent.
2.
- 1.1. The locomotor-inducting factor of the giant African snail, Achatina fulica, was examined.
- 2.2. Snails showed nocturnal circadian behavior in relative humidity at least over 50%. Although the rhythmicity was independent of light and darkness, it was disturbed easily by hydration, and hydrated snails continued to locomote throughout the day. For induction of locomotor behavior, relative humidity over 50% was the fundamental factor and water is shown to be the limiting factor for the endogeneous circadian oscillator.
- 3.3. The integument of snails showed a higher water permeability. Through the integument, hemolymph osmolality changed easily according to hydration and dehydration from about 120 to 400 mOsm/kg H2O. Circadian behavior was induced in snails in which hemolymph osmolality ranged from about 130 to 230 mOsm/kg H2O.
- 4.4. By hydration, hemolymph osmolality in quiescent and estivated snails which have higher osmolality decreased gradually and then they began to locomote according to the degree of dilution, and vice versa. The induction of behavior in these snails was controlled by low hemolymph osmolality.
- 5.5. Together with the endogeneous rhythmicity, water environment was shown to be the key factor for the induction of locomotor behavior.
- 6.6. Based on these results, the mechanisms of the induction of locomotor behavior in terrestrial pulmonates are proposed.
3.
《Comparative biochemistry and physiology. A, Comparative physiology》1979,62(2):279-282
- 1.1. After a single injection of tritium labelled water (THO) into rabbits the specific activity was measured in blood, urine and evaporative water.
- 2.2. The specific activity in urine was similar to that in blood, the specific activity in pulmocutaneous evaporate was 5–8% below that of blood.
- 3.3. The permeability coefficient of the urinary bladder wall for THO was 44 ± 11 cm/sec × 10−6.
- 4.4. Maximum differences in the specific activity between blood and urine due to the accumulation of urine in the bladder were calculated as up to 1.8%.
- 5.5. In the state of equilibrium the specific activity in urine water, but not in evaporative water. can be used for the estimation of total body water.
4.
《Comparative biochemistry and physiology. A, Comparative physiology》1986,83(3):461-475
- 1.1. The buccal-mass responses of the freshwater snail Biomphalaria glabrata to sugars were investigated by means of an olfactometer.
- 2.2. The snails proved very discriminating in their responses. Adults responded significantly to only five (12.2%) of the 41 sugars tested at 10−2 M. Juvenile conspecifics had a broader niche as they responded to eight (47%) of the 17 sugars tested.
- 3.3. Of all the sugars tested, maltose proved to be the most potent phagostimulant.
- 4.4. The possible mechanisms involved in the chemoreception of active sugars and the ecological relevance of the responses shown by the snails to them are discussed.
5.
《Comparative biochemistry and physiology. A, Comparative physiology》1989,92(4):599-600
- 1.1. Freshwater-resident Arctic charr acclimated for 2 months at 8°C, 15% were divided into four experimental groups in July and exposed to 1 and 8°C in 15 and 34% salinity.
- 2.2. Only slight changes in gill Na-K-ATPase activity, blood plasma osmolality and blood plasma concentrations of Cl− and Mg2+ were found for the fish exposed to 1 or 8°C in brackish water.
- 3.3. When exposed to sea-water at 8°C, an increase in osmolality and in concentrations of Cl− and Mg2+ took place during the first 2–3 days, after which it levelled off.
- 4.4. If exposed to sea-water at 1°C, however, marked increases were found for all parameters measured and all the fish were dead within 5 days of exposure.
- 5.5. These results show that freshwater-resident Arctic charr—if acclimated to brackish water—can survive in sea-water during summer if the environmental temperature is not too low.
6.
《Comparative biochemistry and physiology. A, Comparative physiology》1979,62(4):961-964
- 1.1. Nitrogen excretion of germfree snails kept in sterile saline solution during 14 days consists mainly of ammonia and urea in approximately equal amounts.
- 2.2. Uric acid is excreted intermittently being accumulated in snail nephridia.
7.
《Comparative biochemistry and physiology. A, Comparative physiology》1986,83(3):507-513
- 1.1. Hatching Caretta caretta may lose up to 12% of their initial hatched weight from water loss during emergence from the nest.
- 2.2. After subsequent osmotic and excretory water loss in sea water, hatchlings will drink sea water (166 μl 100 g−1 hr−1) and return to their initial weight within 10–15 days, without feeding.
- 3.3. There were no significant changes in plasma osmolarity or sodium levels over this period.
- 4.4. This osmoregulatory strategy is in marked contrast to that seen in the estuarine crocodile, Crocodylus porosus.
8.
《Comparative biochemistry and physiology. A, Comparative physiology》1987,86(1):95-100
- 1.1. Blood volume and plasma biochemical changes and feed and water consumption in response to a hemorrhage by phlebotomy of 30% of the calculated total blood volume with and without replacement of blood volume with physiological saline were determined in juvenile male Coturnix coturnix japonica.
- 2.2. Plasma protein and osmolality decreased rapidly posthemorrhage and did not recover by 72 hr posthemorrhage.
- 3.3. Plasma glucose, Na+ and K+ increased within Ihr postphlebotomy. Plasma Na+ returned to nonphlebotomized levels within 6 hr postphlebotomy.
- 4.4. Saline replacement of blood volume resulted in hypervolemia within 3–5 min postphlebotomy.
- 5.5. Phlebotomized quail receiving no saline recovered blood volume to 0 hr (nonphlebotomized) levels within l hr postphlebotomy.
9.
《Comparative biochemistry and physiology. A, Comparative physiology》1984,77(2):261-266
- 1.1. Flounders transferred abruptly from sea to fresh water displayed a gradual decrease in plasma osmolality for 5–6 days (10–15 mOsm daily). When returned to sea water the osmolality increased to the original level within 1 day.
- 2.2. Heart ventricle cell water content remained unchanged during the acclimations, except for a temporary 1.4% reduction within the first 4 hr of sea water acclimation.
- 3.3. During acclimation to sea water intracellular K+ increased rapidly in parallel with plasma osmolality. During fresh water acclimation, however, cellular K+ decreased rapidly in the first day only, whereas plasma osmolality decreased further.
- 4.4. Cellular taurine remained unchanged during the initial 4 days of fresh water acclimation and then declined 32% within the next 3 days. Upon retransfer to sea water, cellular taurine increased gradually to its original level in the course of 7 days.
10.
《Comparative biochemistry and physiology. A, Comparative physiology》1993,104(4):831-836
- 1.1. Administration of a carbohydrate-rich diet increased haemolymph glucose levels and glycogen concentration in hepatopancreas, mantle and muscle.
- 2.2. Glycogen concentration in tissues decreases after 2 weeks of starvation and haemolymph glucose levels did not change significantly.
- 3.3. However, starvation did not induce a decrease in the intrinsic synthetic capacity in tissues.
- 4.4. Glycogen synthesis in tissues from animals fed with lettuce or a carbohydrate-rich diet, increases with increasing glucose concentration in the media.
- 5.5. However, in mantle slices from snails adapted on a carbohydrate-rich diet, the glycogen synthetic capacity was lower than in slices from snails fed with lettuce.
11.
《Comparative biochemistry and physiology. A, Comparative physiology》1991,98(2):357-360
- 1.1. The effects of alternating current electronarcosis, rectified current electronarcosis and chemical anaesthesia (benzocaine hydrochloride) on plasma electrolytes and on the osmotic pressure of the blood of the freshwater bream Oreochromis mossambicus were evaluated.
- 2.2. Plasma Ca2+, Na+ and K+ concentrations and the osmotic pressure of the blood were monitored over a period of 7 days.
- 3.3. The results showed that the different electrolytes respond differently to the different techniques.
- 4.4. Chemical anaesthesia exhibited the least effects on the parameters studied.
12.
- 1.1. Goldfish were kept in deionized water (DW), DW + Na+ (0.35 mM), DW + K+ (0.05 mM), DW + Ca2+ (2mM) and DW + Mg2+ (0.2 mM). In Ca-free environments, prolactin cells appear unaffected. Stimulated calcium-sensitive cells (pars intermedia) may elaborate a hypercalcemic factor.
- 2.2. Fecal excretion, reduced in all groups, remains noticeable in DW + Ca2+
- 3.3. Ionic losses, very low in all groups, are minimal in DW. Supplementation with K+ increases Na+ loss.
- 4.4. Plasma Na+ Ca2+, and osmolarity decrease in DW, and still more in DW + K+. Ca2+' and Mg2+ partly suppress hyponatremia.
- 5.5. In goldfish kept in DW and subsequently in DW + Ca2+, calcemia increases.
13.
- 1.1. Kinetic aspects of the enzyme UDP-galactose 4-epimerase in crude homogenates of the albumen gland of the snail Lymnae stagnalis were estimated. The mean values of the Km for UDP-galactose and for NAD are 0.343 and 0.097 mM, respectively. The enzyme is inhibited by NADH. It is inactivated by freezing and raised temperature (25°C), but it can be reactivated by NAD.
- 2.2. In the albumen gland the epimerase activity is 10–100 times higher than in other tissues, reflecting the high turnover of glucose to galactose, essential for the synthesis of galactogen in this organ.
- 3.3. In fed snails long day conditions stimulates albumen gland epimerase activity, coinciding with high egg production.
- 4.4. In starved snails a fairly high residual activity of the enzyme is maintained, irrespective of photoperiod or egg production.
- 5.5. Trematode infection leads to a considerable reduction of the epimerase activity.
- 6.6. The results indicate that the epimerase activity in fed snails, when the gland shows a regular release, reflects long-term adaptations (photoperiod). In starved and parasitized snails, when no regular release or product occurs, a basic epimerase activity is maintained. This might be important for a rapid restoration of egg production after the termination of adverse conditions.
14.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1978,59(3):385-389
- 1.1. Aspartate aminotransferase activity in the white muscle of rainbow trout dropped significantly after the animals had received a pyridoxine-deficient diet for 7 days.
- 2.2. Alanine aminotransferase activity in the white muscle of rainbow trout dropped significantly after the animals had been fed on a pyridoxine-deficient diet for 21 days.
- 3.3. Alanine and aspartate aminotransferase activity in the liver of rainbow trout did not drop significantly until the animals had received a pyridoxine-deficient diet for 28 days.
- 4.4. After rainbow trout have received a pyridoxine-deficient diet for 35 days, feeding with complete diet for 7 days is sufficient to restore the aminotransferase activities to the levels observed in control animals.
15.
《Comparative biochemistry and physiology. A, Comparative physiology》1984,77(1):99-103
- 1.1. Final urine is intermittently released from the pneumostome of the pulmonate freshwater snail Lymnaea slagnalis. A technique is described to sample this fluid.
- 2.2. The ionic composition of final urine greatly differs from that of haemolymph; Na+ and Cl− are reabsorbed to a considerable degree. In lettuce fed snails K+ is excreted.
- 3.3. The urine Na+ and Cl− concentrations are about 38 and 31 mM lower, respectively, than the haemolymph concentrations, also when the latter concentrations vary.
16.
《Comparative biochemistry and physiology. A, Comparative physiology》1978,59(2):303-307
- 1.1. In relation to body weight changes resulting from evaporative water losses of up to 37% of initial body weight:
- 1.1.(a) Plasma chloride and potassium concentrations increased in proportion to total body water losses.
- 1.2.(b) Plasma urea concentrations increased at greater rates than expected from the sum of basal synthesis and dehydration.
- 1.3.(c) Plasma sodium concentrations initially increased less rapidly than expected from total body water losses, but by losses of 30% of initial body weight closely approximated predicted concentrations.
- 1.4.(d) Plasma volumes decreased slightly faster than expected, while hematocrits increased as expected.
- 2.2. Skeletal muscles and the ventricular muscles of the heart retained water to greater degrees than expected. Dehydration did not elicit net shifts in Na+ K+, Cl− or amino acids between the intracellular and extracellular compartments in either skeletal muscle or ventricle.
17.
《Comparative biochemistry and physiology. A, Comparative physiology》1984,77(4):695-699
- 1.1. When two male mice fight to establish social rank, the subordinate exhibits marked splenomegaly, reticulocytosis and decreased hematocrit.
- 2.2. Individuals were isolated at weaning, paired 3 weeks later for two 30-min encounter periods daily for up to 3 weeks.
- 3.3. After 2 days and 1 week encounter periods, subordinates had significantly decreased levels of hemoglobin in the kidney compared to dominants and controls.
- 4.4. Subordinates had increased ATP levels in blood after 1, 2 and 3 weeks of encounter, bone marrow erythroid hyperplasia, as well as a substantial increase in the erythropoietin litre and Fe59 incorporation in spleen and blood.
18.
《Comparative biochemistry and physiology. A, Comparative physiology》1992,101(3):451-454
- 1.1. Kidney, oesophagus and gill Na+-K+ ATPase activity and serum Na+, K+ and Cl− concentrations are evaluated in European sea bass during experimental acclimation to fresh water.
- 2.2. Kidney and oesophagus ATPase increase in low salinity and reach a maximum in fresh water.
- 3.3. Gill ATPase decreases during the acclimation trials and rises again to normal values after a 3-week stay in fresh water.
- 4.4. Na+ and K+ serum concentrations decrease during the trials and increase back after a 3-week stay in fresh water.
- 5.5. The correlations between enzymatic activities, serum ion concentrations, morphological changes and environmental salinity are discussed.
19.
《Comparative biochemistry and physiology. A, Comparative physiology》1990,95(1):171-175
- 1.1. Freshwater nonanadromous rainbow trout, Salmo gairdneri, were injected three times a week with either saline, 10μg cortisol/g, 1.0μg thyroxine/g or 10μg cortisol/g + 1.0μg thyroxine/g during a period of 28 days (12 injections). A separate group was derived as a subgroup from the thyroxine group on day 14 and received Cortisol + thyroxine from day 14 until day 28 (six injections).
- 2.2. Gill chloride cell number and Na+/K+-ATPase activity increased by cortisol treatment, the changes being significant on days 7 and 14, respectively.
- 3.3. Thyroxine treatment did not affect gill Na+/K+-ATPase activity or chloride cell number directly. Neither did it modify the stimulatory effect of cortisol on these parameters.
- 4.4. Muscle water decreased in cortisol-treated fish and increased in thyroxine-treated fish, while no changes were observed in the combined hormone groups.
- 5.5. No changes were observed in plasma chloride in any group during the experiment.
- 6.6. The results demonstrate a putative role of cortisol in stimulating hypo-osmoregulatory mechanisms and suggest that thyroxine is without a direct or a supportive effect for cortisol action.
20.
- 1.1. The effects of 2% saline imbibition and water deprivation on the water balance of the gerbil were compared.
- 2.2. The unchanged fluid intake and losses, body weight and several blood indices suggested little alteration in the state of hydration after saline imbibition.
- 3.3. After 5 days water deprivation the animals lost weight and evidence of haemoconcentration was observed. These changes took place despite reductions in water loss (via the urine and faeces) and evidence of secretion of vasopressin and the two principal acidic neural lobe proteins.
- 4.4. The gerbil appeared to be better adapted to water stress induced by saline imbibition than by water deprivation and this may be related to its habitat in Northern Asia.