四川甘孜曲丝藻科硅藻中国新记录

在四川省甘孜藏族自治州进行硅藻多样性调查时,发现了单壳缝目硅藻中国新记录8种1变种(隶属于4属),分别为:原子曲丝藻Achnanthidium atomus Monnier,Lange-Bertalot&Ector、施特劳宾曲丝藻A.straubianum (Lange-Bertalot) Lange-Bertalot、亚显曲丝藻A. pseudoconspicuum (Foged) Jüttner&Cox、半孔曲丝藻A. semiapertum (Guermeur) Andresen,Stoermer&Kreis、阿雷塔斯真卵形藻Eucocconeis aretassii(Manguin) Lange-Bertalot、披针平面藻微小变种Planothidium lanceolatum var. minor Cleve、厄氏平面藻P.oestrupii (Cleve-Euler) Edlund、球囊沙生藻Psammothidium sacculus (Carter) Bukhtiyarova和双生沙生藻P.didymum (Hustedt) Bukhtiyarova&Round。利用光学显微镜(LM)及扫描电镜(SEM)对8种1变种进行观察,并对其形态特征和生境进行了详细描述。     

青海省冬虫夏草资源适宜性区划分析

冬虫夏草是青藏高原的珍稀物种,具有较高的药用和经济价值,然而其资源的数量特征、空间分布格局及适宜度尚不清晰。本研究以青海省冬虫夏草资源生境条件为依据,以海拔、植被、土壤、气温和降水量作为主要指标,利用地理信息系统(geographic information system,GIS)的空间分析方法对青海省冬虫夏草资源适宜性及空间分布进行分析,并用野外实地采样进行验证。研究表明：玉树州的玉树市、杂多县、称多县、曲麻莱县;果洛州的玛沁县、达日县、甘德县等区域多处在适宜区;海北州的祁连县、刚察县,黄南州的泽库县、河南县、同仁县,玉树州的治多县、囊谦县,海南州的兴海县、同德县、贵南县及其他产冬虫夏草县的部分区域为较适宜区;西宁市的大通县,海东市的互助县和海西州的低海拔地区为不适宜区。整个青藏高原具有冬虫夏草分布面积占当地县域国土面积的76.2%。在青海省具有冬虫夏草分布的地区(市/州)中,玉树州和果洛州冬虫夏草分布的面积占当地县域国土面积的88.3%-99.6%,海北州、海东市、海南州、海西州、黄南州和西宁市冬虫夏草分布面积占当地县域国土面积的30.2%-96.5%。综合区划图能比较真实地反映青海省冬虫夏草资源的空间分布格局,区划结果与实地调查结果相符,表明利用空间分析方法进行冬虫夏草资源的适宜性分布区域的划分是可行的,该研究为合理开发和科学利用冬虫夏草野生资源提供理论依据。     

Genetic variation and population structure of “Zangli” pear landraces in Tibet revealed by SSR markers

Pear (Pyrus L.) is one of the most important fruits in temperate regions. Even in the mountainous border region of Tibet, Sichuan, and Yunnan Provinces (China), a variety of highly adapted pear landraces were discovered in Tibetan Autonomous Prefecture and were named “Zangli pears.” Here, we analyzed genetic diversity of 29 genotypes of Zangli distributed in Tibetan Region, together with 30 genotypes of sand pear (P. pyrifolia) in border regions, and 8 genotypes of P. ussuriensis as out groups, using simple sequence repeats (SSR) markers. Twenty-eight SSR markers displayed high polymorphism with a total of 202 alleles. Cluster analysis revealed a close genetic relationship between Zangli and sand pears, while population structure analysis revealed that gene flow between the western and eastern side of the Jin-sha River was restricted by geographic isolation. Diversity statistics for five geographic groups implied a migration event from Sichuan and Yunnan Provinces to the border region of western Sichuan, eastern Tibet, and northwestern Yunnan Provinces. Six specific genetic alleles in Zangli pear landraces might be related to their adaption to severe environmental conditions.     

基于最大熵模型(MaxEnt)预测暗紫贝母的潜在分布

暗紫贝母(Fritillaria unibracteata)干燥的鳞茎是市场名贵药材"川贝"的主要来源,目前由于人们的过度挖采及环境的恶化,使得其野生资源已近枯竭,被列为国家三级濒危保护药材物种。该研究通过收集暗紫贝母的地理分布点经纬度,结合26项生态因子,运用最大熵模型(MaxEnt)并结合地理信息系统(ArcGIS),对其在中国的潜在分布区域进行了预测。结果表明:暗紫贝母的潜在适生区主要分布在四川西部和北部、青海南部、甘肃南部,其中四川阿坝藏族羌族自治州的理县、茂县、松潘县、红原县、黑水县等地区,青海省果洛藏族自治州的久治县、玛沁县、同德县、兴海县、河南县地区以及甘肃省甘南藏族自治州地区是暗紫贝母最佳适生区。此外,在西藏和云南也有零星的分布。对暗紫贝母的分布贡献率较大的主要生态因子有5个,分别是海拔(40.8%),年均降水量(28%),1月最高温度(7.1%),最干季平均温度(6.6%)和昼夜温差日均值(6.6%)。其中,海拔为2700~4 500m、年均降水量为400~1 400mm,是暗紫贝母最适宜生长的生态位参数。该研究结果为暗紫贝母的野生抚育和人工栽培提供了重要的科学依据。     

Intra-specific diversity of the chemical composition of Ligularia lamarum in the Hengduan Mountains,China: The structures of four new eremophilanes and a new seco-eremophilane

Chemical compositions and internal transcribed spacer (ITS) sequences of five samples of Ligularia lamarum collected in Sichuan Province, China, were analyzed. Fourteen compounds, including four new eremophilanes and one new seco-eremophilane, were isolated and their structures were elucidated by spectroscopic methods. Intra-specific diversity in the chemical composition was found to be higher than previously known. The result of DNA analysis suggested that one of the samples was introgressed, although its chemical composition was typical of L. lamarum.     

Molecular identification and genetic-polymorphism analysis of Fasciola flukes in Dali Prefecture,Yunnan Province,China

This study aimed to identify species of Fasciola flukes in Dali Prefecture (Yunnan Province, China) and analyze their genetic diversity. Fasciola flukes (n = 122) were collected from cattle livers in a farmers' market in Xiaguan Town, Dali Prefecture. Nucleotide sequences of ribosomal internal transcribed spacer (ITS) as well as nicotinamide adenine dinucleotide dehydrogenase subunit 1 (ND1) and mitochondrial cytochrome c oxidase subunit 1 (CO1) were amplified, sequenced, and subjected to homology analysis. The heterozygosity ratios of different ITS alleles were determined using the peak-height ratio of heterozygous loci. Multiplex PCR analysis of the nuclear protein coding gene, phosphoenolpyruvate carboxykinase (pepck), was used to identify Fasciola species. Multiple ND1 sequence alignments enabled further genetic diversity analysis of regional Fasciola flukes. Seven ITS sequences belonged to F. hepatica and 115 belonged to Fh/Fg heterozygous flukes. Sequencing analysis of heterozygous flukes revealed 11 heterozygous loci with double peaks, with significantly variable ratios among individuals. ND1 and CO1 results indicated that one specimen was identical to F. hepatica, while 121 specimens were identical to F. gigantica or contained one variable site. Multiplex PCR results for pepck showed that double bands for F. hepatica and F. gigantica were amplified from Dali Fasciola specimens; hence, they were all heterozygous. By combining ITS, ND1, and CO1 sequences with multiplex pepck PCR results, all 122 specimens were identified as Fh/Fg heterozygous Fasciola flukes. Our experimental results preliminarily confirmed a high degree of Fh/Fg heterozygosity among Fasciola flukes in the Dali area. Selecting multiple molecular markers for concurrent analysis will provide more comprehensive and accurate genetic information.     

Prevalence,Associated Risk Factors,and Phylogenetic Analysis of Toxocara vitulorum Infection in Yaks on the Qinghai Tibetan Plateau,China

Toxocara vitulorum has been rarely reported in yaks at high altitudes and remote areas of Sichuan Province of Tibetan Plateau of China. The current study was designed to investigate the prevalence, associated risk factors, and phylogenetic characteristics of T. vitulorum in yak calves on the Qinghai Tibetan plateau. Fecal samples were collected from 891 yak calves and were examined for the presence of T. vitulorum eggs by the McMaster technique. A multivariable logistic regression model was employed to explore variables potentially associated with exposure to T. vitulorum infection. T. vitulorum specimens were collected from the feces of yaks in Hongyuan of Sichuan Province, China. DNA was extracted from ascaris. After PCR amplification, the sequencing of ND1 gene was carried out and phylogenetic analyses was performed by MEGA 6.0 software. The results showed that 64 (20.1%; 95% CI 15.8–24.9%), 75 (17.2; 13.8–21.1), 29 (40.9; 29.3–53.2), and 5 (7.6; 2.5–16.8) yak calves were detected out to excrete T. vitulorum eggs in yak calve feces in Qinghai, Tibet, Sichuan, and Gansu, respectively. The present study revealed that high infection and mortality by T. vitulorum is wildly spread on the Qinghai Tibetan plateau, China by fecal examination. Geographical origin, ages, and fecal consistencies are the risk factors associated with T. vitulorum prevalence by logistic regression analysis. Molecular detection and phylogenetic analysis of ND1 gene of T. vitulorum indicated that T. vitulorum in the yak calves on the Qinghai Tibetan plateau are homologous to preveiously studies reported.     

三江源区冬虫夏草资源适宜性空间分布

冬虫夏草(Ophiocordyceps sinensis)是生长在青藏高原高寒地区的一种珍稀物种资源,具有极高的药用和经济价值,然而冬虫夏草资源的数量特征与空间格局尚不明确。以三江源区为例,结合冬虫夏草的生境条件,选取适宜冬虫夏草生长的生态环境指标,利用地理信息系统(GIS)中的空间分析方法对冬虫夏草资源的适宜性空间分布进行了研究,并用野外实地采样和入户调研数据进行验证。结果表明:三江源区冬虫夏草资源主要分布在东南部和中南部,分布面积约为18.45×10~4km~2,占该区总面积的50.74%。达日县、甘德县、玉树县、称多县、玛沁县、班玛县、久治县这7个县冬虫夏草适宜性面积占到了该县50%以上的面积,是三江源区冬虫夏草资源的主产区。基于GIS空间分析的结果与实地调查结果相符,说明利用空间分析方法划分冬虫夏草资源的适宜性分布区域是可行的,可为系统的全面调查冬虫夏草资源的分布及保护与合理开发利用冬虫夏草资源提供科学依据。     

四川秋季发生的两种羊肚菌生境调查与鉴定

为了寻找四川秋季发生的羊肚菌资源,了解其产地环境及种属关系,探寻羊肚菌在秋季发生的适生条件,对四川省凉山州盐源县、会东县和冕宁县的羊肚菌发生地开展了生境调查,共采集到11份羊肚菌标本。采用形态学和分子鉴定相结合的方法,对标本进行鉴定,结果表明所采集的标本被鉴定为2种羊肚菌,即七妹羊肚菌Morchella septimelata（Mel-7）和Morchella sp.（Mes-16）,其中,七妹羊肚菌为四川新记录种。通过生境调查发现,秋季发生的羊肚菌适生环境为山地林下陡坡,可见,四川秋季发生的羊肚菌有一定的特殊性。     

Nuclear DNA assay of the wild endangered medicinal and aromatic Indian Himalayan Valeriana jatamansi germplasm with multiple DNA markers: implications for genetic enhancement,domestication and ex situ conservation

Population genetic analysis in the important endangered medicinal and aromatic plant species, Valeriana jatamansi, provided, first time, insights into the identification of novel sources of genetic variation as an aid for improvement and domestication, and for optimizing conservation strategies. The 75 genotypes of V. jatamansi were collected from 36 locations across northeast to northwest Indian Himalayas of ~1,000 km, harbouring variable climatic and ecological conditions and rugged rocky terrain. The known protocols for DNA extraction failed to yield quality DNA in good quantity. A new protocol was standardized for this purpose. All the three (RAPD, ISSR, AFLP) DNA markers were successful in detecting polymorphism in V. jatamansi genotypes, and the ISSR marker, vis-à-vis RAPD and AFLP markers, generated the highest level of polymorphism. The RAPD, ISSR and AFLP fingerprints with 23 and 15 primers and 8 primer combinations, respectively, revealed 85.8, 89.0 and 67.7 % polymorphism among 141, 91 and 37 genetic loci amplified from the 75 genotypes, respectively. The AMOVA analysis of AFLP (55.0, 8.3, 36.7 %), RAPD (57.4, 11.9, 30.6 %) and ISSR (76.0, 4.8, 19.1 %) data indicated that more variation existed in differences in genotypes within populations than between populations within a region and between regions, respectively. The present comprehensive input will assist in effective management and (or) devising conservation strategies of this important medicinal plant species. This study reports the start of a molecular biology programme targeting nuclear genome of V. jatamansi, the genetics of which is very little known.     

Prevalence and spatial distribution characteristics of human echinococcosis in China

BackgroundEchinococcosis is a zoonotic parasitic disease caused by larval stages of cestodes belonging to the genus Echinococcus. The infection affects people’s health and safety as well as agropastoral sector. In China, human echinococcosis is a major public health burden, especially in western China. Echinococcosis affects people health as well as agricultural and pastoral economy. Therefore, it is important to understand the prevalence status and spatial distribution of human echinococcosis in order to advance our knowledge of basic information for prevention and control measures reinforcement.MethodsReport data on echinococcosis were collected in 370 counties in China in 2018 and were used to assess prevalence and spatial distribution. SPSS 21.0 was used to obtain the prevalence rate for CE and AE. For statistical analyses and mapping, all data were processed using SPSS 21.0 and ArcGIS 10.4, respectively. Chi-square test and Exact probability method were used to assess spatial autocorrelation and spatial clustering.ResultsA total of 47,278 cases of echinococcosis were recorded in 2018 in 370 endemic counties in China. The prevalence rate of human echinococcosis was 10.57 per 10,000. Analysis of the disease prevalence showed obvious spatial positive autocorrelation in globle spatial autocorrelation with two aggregation modes in local spatial autocorrelation, namely high-high and low-high aggregation areas. The high-high gathering areas were mainly concentrated in northern Tibet, western Qinghai, and Ganzi in the Tibetan Autonomous Region and in Sichuan. The low-high clusters were concentrated in Gamba, Kangma and Yadong counties of Tibet. In addition, spatial scanning analysis revealed two spatial clusters. One type of spatial clusters included 71 counties in Tibet Autonomous Region, 22 counties in Qinghai, 11 counties in Sichuan, three counties in Xinjiang Uygur Autonomous Region, two counties in Yunnan, and one county in Gansu. In the second category, six types of spatial clusters were observed in the counties of Xinjiang Uygur Autonomous Region, and the Qinghai, Gansu, and Sichuan Provinces.ConclusionThis study showed a serious prevalence of human echinococcosis with obvious spatial aggregation of the disease prevalence in China. The Qinghai-Tibet Plateau is the "hot spot" area of human echinococcosis in China. Findings from this study indicate that there is an urgent need of joint strategies to strengthen efforts for the prevention and control of echinococcosis in China, especially in the Qinghai-Tibet Plateau.     

Mitochondrial phylogeography and genetic diversity of Tibetan gazelle (Procapra picticaudata): implications for conservation

The Tibetan gazelle (Procapra picticaudata) is a threatened species and distributed on the Qinghai-Tibet Plateau of China (Qinghai Province, Tibet Autonomous Region and the adjacent Gansu Province, Sichuan Province, and Xinjiang Uigur Autonomous Region). Small peripheral populations of Tibetan gazelle were once found in northern Sikkim and Ladakh, but now these are close to extinction. To describe the evolutionary history and to assess the genetic diversity within this monotypic species and population structure among different geographic locations in China, we sequenced mitochondrial DNA from the control region (CR) and cytochrome (cyt) b gene for 46 individuals from 12 geographic localities in Qinghai, Tibet, Xinjiang, Gansu, and Sichuan. A total of 25 CR haplotypes and 16 cyt b haplotypes were identified from these gazelle samples. CR haplotype diversity (0.98+/-0.01) and nucleotide diversity (0.08+/-0.009) were both high. Phylogenetic trees indicate that the Tibetan gazelle in China can be divided into three main clades: Tibet, Sichuan (SCH) and Qinghai-Arjin Shan-Kekexili (QH-ARJ-KKXL). Analysis of molecular variance (AMOVA) and network analysis consistently support this geographic structure in both datasets. Significant differentiation between populations argues for the presence of management units (MUs). Such differentiation may reflect a geographic separation resulting from the uplift of the Qinghai-Tibet Plateau during the Late Pliocene and Pleistocene. Mismatch distribution analysis implies that Tibetan gazelle has undergone complex population changes. We suggest that the present population structure has resulted from habitat fragmentation during the recent glacial period on the Qinghai-Tibet Plateau and population expansion from glacial refugia after the glacial period. It is likely that the present populations of Tibetan gazelle exhibit a pattern reminiscent of several bottlenecks and expansions in the recent past.     

基于MaxEnt的西藏飞蝗在中国的适生区预测

西藏飞蝗是青藏高原本地特有物种,为青稞和牧草的重要害虫,近年来危害范围有扩大蔓延趋势。研究并明确西藏飞蝗在中国的适生区域,对制定该虫的早期监测、预警及控制措施意义重大。近年来最大熵理论在物种适生研究领域得到广泛应用,基于西藏飞蝗的分布信息和环境变量,采用MaxEnt生态位模型和ArcGIS对其在中国的潜在分布区进行预测,用ROC曲线检测模型精度、刀切法(jackknife test)筛选主导环境变量及其适宜值。两次模拟的AUC值分别为0.996和0.993,预测结果与实际拟合度很高。西藏飞蝗在中国的高适生区主要位于四川的甘孜州,西藏的昌都地区、林芝地区、山南地区及拉萨市,中适生区则以高适生区为中心向外扩散,集中在青藏高原东部地区。海拔、8月份平均雨量、1月份平均雨量、等温性、12-2月份的平均温度是影响西藏飞蝗潜在分布的主要环境变量。     

黑姜的化学成分、药理作用及毒理学研究进展

黑姜（Kaempferia parviflora）是东南亚地区广泛栽培的民间药用植物,被用于制造食品、化妆品和医药品。黑姜在我国西双版纳傣族自治州亦有较长的民间药用栽培历史。根茎是黑姜重要的药用部位,主要含有黄酮类物质。许多药理作用研究表明黑姜具有抗肿瘤、增强男性性能力、抗炎、抗氧化、抗真菌、抗病毒、神经保护、血管舒张和心脏保护活性、经皮促渗等作用。对黑姜的化学成分、药理作用及毒理学研究进行综述,以期为进一步深入研究和开发利用黑姜资源提供帮助。     

基于细胞色素b基因的中国岩羊不同地理种群遗传差异分析

为了揭示中国岩羊不同地理种群的遗传差异,探讨岩羊亚种分化的分子机制,采用中国岩羊不同地理种群的细胞色素b(Cyt b)基因的全序列,分析了碱基变异情况、遗传距离以及核苷酸序列差异。用最大似然法和贝叶斯法构建分子系统树并对获得的拓扑结构进行分析。结果发现,西藏亚种与四川亚种Cyt b基因平均序列差异为4.2%(±0.007),处于偶蹄目亚种的序列差异范围内,支持了目前对岩羊西藏亚种的分类地位。四川亚种内部各地理种群之间的遗传距离(0.033±0.0 111)与它们分别到西藏种群的遗传距离(0.042±0.007)差异不显著(t=1.824,P=0.084),说明四川亚种内部各地理种群间已经发生较显著的遗传分化。其中,四川、甘肃和青海种群亲缘关系较近,并与四川亚种内部的其它种群已产生了显著的遗传分化。因此认为四川亚种内部各地理种群的种下分化需要深入研究。     

Potential distribution of Notopterygium incisum Ting ex H. T. Chang and its predicted responses to climate change based on a comprehensive habitat suitability model

Notopterygium incisum Ting ex H. T. Chang is a rare and endangered traditional Chinese medicinal plant. In this research, we built a comprehensive habitat suitability (CHS) model to analyze the potential suitable habitat distribution of this species in the present and future in China. First, using nine different algorithms, we built an ensemble model to explore the possible impacts of climate change on the habitat distribution of this species. Then, based on this model, we built a CHS model to further identify the distribution characteristics of N. incisum‐suitable habitats in three time periods (current, 2050s, and 2070s) while considering the effects of soil and vegetation conditions. The results indicated that the current suitable habitat for N. incisum covers approximately 83.76 × 10³ km², and these locations were concentrated in the Tibet Autonomous Region, Gansu Province, Qinghai Province, and Sichuan Province. In the future, the areas of suitable habitat for N. incisum would significantly decrease and would be 69.53 × 10³ km² and 60.21 × 10³ km² in the 2050s and 2070s, respectively. However, the area of marginally suitable habitat would remain relatively stable. This study provides a more reliable and comprehensive method for modelling the current and future distributions of N. incisum, and it provides valuable insights for highlighting priority areas for medicinal plant conservation and resource utilization.     

四川省石渠县西藏盘羊种群与分布现状调查

西藏盘羊长期被怀疑已在四川省甘孜藏族自治州绝灭,直到20世纪末在石渠县发现3只个体。我们于2013-2018年在甘孜州12县34乡开展了访问和实地调查,结果仅在石渠县发现实体,共发现24群321头,集群大小1~41头/群,平均为（13.4 ± 11.4 ）只。其中全雄性14群,集群大小1~15只,平均（7.4 ± 4.6）只;雌幼混合8群,集群大小9~41只,平均（24.6 ± 13.0） 只;雌雄幼混合群2群,分别为8只、12只。共记录到成年个体238头,性比略偏雌 (雌雄比为128∶110);1~2龄个体38头,0~1龄个体45头。根据在真达-色须-尼嘎-洛须等乡镇的历年重复调查,保守估计适宜栖息地内盘羊的种群密度为每平方千米0.33~0.57只。调查区域内盘羊面临的威胁因素包括过度放牧、护牧犬、狼(Canis lupus)、雪豹(Panthera uncia)和金雕(Aquila chrysaetos)。据此,为保护石渠县西藏盘羊,我们建议进行更深入的分布、栖息地和种群调查,并开展以盘羊为主题的宣传教育和生态旅游。     

An Outbreak of Type Π Vaccine-Derived Poliovirus in Sichuan Province,China: Emergence and Circulation in an Under-Immunized Population

Background

During August 2011–February 2012, an outbreak of type Π circulating vaccine-derived poliovirus (cVDPVs) occurred in Sichuan Province, China.

Methods

A field investigation of the outbreak was conducted to characterize outbreak isolates and to guide emergency response. Sequence analysis of poliovirus capsid protein VP1 was performed to determine the viral propagation, and a coverage survey was carried out for risk assessment.

Results

One clinical compatible polio case and three VDPV cases were determined in Ngawa County, Ngawa Tibetan and Qiang Autonomous Prefecture, Sichuan Province. Case patients were unimmunized children, 0.8–1 years old. Genetic sequencing showed that the isolates diverged from the VP1 region of the type Π Sabin strain by 5–12 nucleotides (nt) and shared the same 5 nt VP1 substitutions, which indicate single lineage of cVDPVs. Of the 7 acute flaccid paralysis cases (all>6 months) reported in Ngawa Prefecture in 2011, 4 (57.1%) cases (including 2 polio cases) did not receive oral attenuated poliovirus vaccine. Supplementary immunization activities (SIAs) were conducted in February–May, 2012, and the strain has not been isolated since.

Conclusion

High coverage of routine immunization should be maintained among children until WPV transmission is globally eradicated. Risk assessments should be conducted regularly to pinpoint high risk areas or subpopulations, with SIAs developed if necessary.     

Laccaria (Agaricomycetes,Basidiomycota) from Tibet (Xizang Autonomous Region,China)

Species of Laccaria are described from the eastern Himalayas, in the Xizang Autonomous Region of China, more commonly known as Tibet. Specimens were collected during several expeditions over a 12-year time span. Nuclear ribosomal internally transcribed spacer regions 1 and 2 including and 5.8S (ITS) as well as the 5' end of the large subunit (28S) sequence data were generated for 22 specimens from Tibet and analyzed in a dataset of 115 Laccaria samples. The results documented seven species from this region, five of which represent currently undescribed species. The taxonomy of Tibetan Laccaria is discussed, five new species are proposed, and an artificial key that includes extralimital species from Asia is provided.     

川西云杉天然种群表型多样性

为了揭示川西云杉(Picea balfouriana)天然种群表型变异程度和变异规律, 以四川甘孜藏族自治州天然分布的川西云杉为研究对象, 调查了12个种群360个单株的种实和针叶表型性状, 采用变异系数、巢式方差分析、相关分析和聚类分析等方法, 分析了种群间和种群内表型多样性。结果表明: 川西云杉表型性状在种群间和种群内存在极其丰富的多样性, 种群间表型分化系数均值为36.53%, 小于种群内变异(63.47%); 球果、针叶、种鳞和种翅的表型分化系数均值分别为47.15%、31.93%、21.89%和45.14%, 球果、针叶、种鳞和种翅4类表型性状的变异系数分别为12.56%、22.16%、12.61%和16.53%, 种鳞的变异系数和表型分化系数最小, 表明其性状最为稳定; 12个表型性状间多数呈显著或极显著正相关, 球果长、球果径、针叶长和种鳞长为川西云杉重要的表型性状。川西云杉表型变异在空间分布上呈现以经度(球果和针叶)和纬度(种鳞和种翅)并存的单向变异模式; 利用种群间欧氏距离进行聚类分析, 可以把川西云杉12个种群划分为3类。