共查询到20条相似文献,搜索用时 468 毫秒
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An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition 总被引:1,自引:0,他引:1
Gregory PA Bracken CP Smith E Bert AG Wright JA Roslan S Morris M Wyatt L Farshid G Lim YY Lindeman GJ Shannon MF Drew PA Khew-Goodall Y Goodall GJ 《Molecular biology of the cell》2011,22(10):1686-1698
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Xiao Ling Li Toshifumi Hara Youngeun Choi Murugan Subramanian Princy Francis Sven Bilke Robert L. Walker Marbin Pineda Yuelin Zhu Yuan Yang Ji Luo Lalage M. Wakefield Thomas Brabletz Ben Ho Park Sudha Sharma Dipanjan Chowdhury Paul S. Meltzer Ashish Lal 《Molecular and cellular biology》2014,34(3):533-550
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YoonSeok Choi Hoe Suk Kim Jisu Woo Eun Hye Hwang Kyoung-Won Cho Soonhag Kim Woo Kyung Moon 《PloS one》2014,9(7)
The epithelial-mesenchymal transition (EMT) plays important roles in tumor progression to metastasis. Thus, the development of an imaging probe that can monitor transient periods of the EMT process in live cells is required for a better understanding of metastatic process. Inspired by the fact that the mRNA expression levels of zinc finger E-box-binding homeobox 1 (ZEB1) increase when cells adopt mesenchyme characteristics and that microRNA-200a (miR-200a) can bind to ZEB1 mRNA, we conjugated molecular beacon (MB) mimicking mature miR-200a to magnetic nanoparticles (miR-200a-MB-MNPs) and devised an imaging method to observe transitional changes in the cells during EMT. Transforming growth factor-β1 treated epithelial cells and breast cancer cell lines representing both epithelial and mesenchymal phenotypes were used for the validation of miR-200a-MB-MNPs as an EMT imaging probe. The real-time imaging of live cells acquired with the induction of EMT revealed an increase in fluorescence signals by miR-200a-MB-MNPs, cell morphology alterations, and the loss of cell-cell adhesion. Our results suggest that miR-200a-MB-MNPs can be used as an imaging probe for the real-time monitoring of the EMT process in live cells. 相似文献
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Yi Liu Yan Li Qi Xu Wenxi Yao Qiuyun Wu Jiali Yuan Weiwen Yan Tiantian Xu Xiaoming Ji Chunhui Ni 《生物化学与生物物理学报:疾病的分子基础》2018,1864(2):420-431
Long non-coding RNAs (lncRNAs) are important signal transduction regulators that act by various patterns. However, little is known about the molecular mechanisms of lncRNA related pathways in occupational lung fibrosis. Our previous study found that epithelial-mesenchymal transition (EMT) was one of the key events in silica-induced pulmonary fibrosis. This study showed that the lncRNA-ATB promoted EMT by acting as a miR-200c sponge. miR-200c was identified by miRNA array as a potential target of lncRNA-ATB and verified by dual luciferase reporter gene together with RNA pull-down assays. Moreover, our findings demonstrated that lncRNA-ATB is abundantly expressed during EMT of lung epithelial cells, which contributes to decreased levels of miR-200c. miR-200c targeted ZEB1 to relief silicosis by blocking EMT in vivo and in vitro. The results also suggested M2 macrophages secreted transforming growth factor-β1 (TGF-β1) to induce EMT process by activating lncRNA-ATB in epithelial cells. Collectively, silica-stimulated macrophages secreted TGF-β1 to induce lncRNA-ATB in epithelia cells, promoting EMT by binding with miR-200c and releasing ZEB1. These observations provide further understanding of the regulatory network of silica-induced pulmonary fibrosis and identify new therapeutic targets hopefully. 相似文献
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