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简述了工业生物技术领域在过程科学方面的研究现状及发展趋势,从"细胞群体效应及过程放大原理"、"工业生物过程物质和能量传递与生物转化规律"、"工业生物过程优化新方法"等3个方面介绍了我国工业生物技术过程科学的重点进展和在国际上的地位,最后从"生物原料高效转化"、"生物转化过程物质和能量协调和匹配"、"生物过程强化"、"生物过程系统集成"等方面提出了工业生物技术在过程科学研究方面未来的战略方向。 相似文献
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<正>生物过程工业(bioprocess industry)包括传统的发酵工业和现代生物技术工业,是国民经济可持续发展的支柱之一。生物过程关键技术和装备是发展我国生物产业的重要支撑。国务院颁布的《国家中长期科学和技术发展规划纲要(2006~2020年)》明确将新一代工业生物技术和过程工业的绿色化、自动 相似文献
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简述了工业生物技术的发展背景和意义,分析了基因组学和功能基因组学发展对工业生物技术的推动作用,重点介绍了本期专刊发表的代谢工程、发酵工程以及工业酶与生物催化领域的17篇论文。 相似文献
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固态发酵工程与生物饲料添加剂的生产 总被引:1,自引:0,他引:1
发酵工程是生物技术的关键技术,固态发酵作为发酵工程的重要部分,由于能源危机与环境问题的日益严重,越来越受到人们的重视,并在资源环境应用研究方面取得了重要进展。着重综述了固态发酵技术在生物饲料添加剂生产中的应用,介绍了固态发酵工程技术的新进展。 相似文献
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《生物产业技术》2016,(3)
正在讨论实现生物技术产业化时,认为由于从基因、细胞到生物反应器操作的生物过程高度复杂性,必须改变传统的数据处理思维方式。建议采用大数据分析方法和工业4.0,打破生命科学上游研究到生物制造下游研究的多学科技术壁垒,其大数据分析的4V特征和3个观念转变是生物过程研究的基本出发点。总结了笔者研究组近几十年的研究成果,分析了发酵过程多尺度理论方法与大数据分析理念,由此探索形成新的概念、理论、方法与装备技术。其中最重要的技术进展标志是生物反应器由原来的参数自动控制演变为实现过程优化与放大决策的智能控制,以及"数据超载"情况下的"信息→相关→因果→知识"的研究过程。 相似文献
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Radhakrishnan Mahadevan Anthony P. Burgard Iman Famili Steve Van Dien Christophe H. Schilling 《Biotechnology and Bioprocess Engineering》2005,10(5):408-417
Increasing numbers of value added chemicals are being produced using microbial fermentation strategies. Computational modeling
and simulation of microbial metabolism is rapidly becoming an enabling technology that is driving a new paradigm to accelerate
the bioprocess development cycle. In particular, constraint-based modeling and the development of genome-scale models of industrial
microbes are finding increasing utility across many phases of the bioprocess development workflow. Herein, we review and discuss
the requirements and trends in the industrial application of this technology as we build toward integrated computational/experimental
platforms for bioprocess engineering. Specifically we cover the following topics: (1) genome-scale models as genetically and
biochemically consistent representations of metabolic networks; (2) the ability of these models to predict, assess, and interpret
metabolic physiology and flux states of metabolism; (3) the model-guided integrative analysis of high throughput ‘omics’ data;
(4) the reconciliation and analysis of on- and off-line fermentation data as well as flux tracing data; (5) model-aided strain
design strategies and the integration of calculated biotransformation routes; and (6) control and optimization of the fermentation
processes. Collectively, constraint-based modeling strategies are impacting the iterative characterization of metabolic flux
states throughout the bioprocess development cycle, while also driving metabolic engineering strategies and fermentation optimization. 相似文献
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Fabricio A. Chiappini Silvana Azcarate Mirta R. Alcaraz Ángela G. Forno Hector C. Goicoechea 《Biotechnology progress》2021,37(4):e3173
In this investigation, the fermentation step of a standard mammalian cell-based industrial bioprocess for the production of a therapeutic protein was studied, with particular emphasis on the evolution of cell viability. This parameter constitutes one of the critical variables for bioprocess monitoring since it can affect downstream operations and the quality of the final product. In addition, when the cells experiment an unpredictable drop in viability, the assessment of this variable through classic off-line methods may not provide information sufficiently in advance to take corrective actions. In this context, Process Analytical Technology (PAT) framework aims to develop novel strategies for more efficient monitoring of critical variables, in order to improve the bioprocess performance. Thus, in this work, a set of chemometric tools were integrated to establish a PAT strategy to monitor cell viability, based on fluorescence multiway data obtained from fermentation samples of a particular bioprocess, in two different scales of operation. The spectral information, together with data regarding process variables, was integrated through chemometric exploratory tools to characterize the bioprocess and stablish novel criteria for the monitoring of cell viability. These findings motivated the development of a multivariate classification model, aiming to obtain predictive tools for the monitoring of future lots of the same bioprocess. The model could be satisfactorily fitted, showing the non-error rate of prediction of 100%. 相似文献
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Schweder T 《Biotechnology journal》2011,6(8):926-933
The optimization and the scale up of industrial fermentation processes require an efficient and possibly comprehensive analysis of the physiology of the production system throughout the process development. Furthermore, to ensure a good quality control of established bioprocesses, on-line analysis techniques for the determination of marker gene expression are of interest to monitor the productivity and the safety of bioprocesses. A prerequisite for such analyses is the knowledge of genes, the expression of which is critical either for the productivity or for the performance of the bioprocess. This work reviews marker genes that are specific indicators for stress- and nutrient-limitation conditions or for the physiological status of the bacterial production hosts Bacillus subtilis, Bacillus licheniformis and Escherichia coli. The suitability of existing gene expression analysis techniques for bioprocess monitoring is discussed. Analytical approaches that enable a robust and sensitive determination of selected marker mRNAs or proteins are presented. 相似文献
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Yield and productivity are critical for the economics and viability of a bioprocess. In metabolic engineering the main objective
is the increase of a target metabolite production through genetic engineering. Metabolic engineering is the practice of optimizing
genetic and regulatory processes within cells to increase the production of a certain substance. In the last years, the development
of recombinant DNA technology and other related technologies has provided new tools for approaching yields improvement by
means of genetic manipulation of biosynthetic pathway. Industrial microorganisms like Escherichia coli, Actinomycetes, etc. have been developed as biocatalysts to provide new or to optimize existing processes for the biotechnological production
of chemicals from renewable plant biomass. The factors like oxygenation, temperature and pH have been traditionally controlled
and optimized in industrial fermentation in order to enhance metabolite production. Metabolic engineering of bacteria shows
a great scope in industrial application as well as such technique may also have good potential to solve certain metabolic
disease and environmental problems in near future. 相似文献
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In recent years, the development of advanced systems for bioprocess monitoring and control has become an area of intensive research. Along with traditional techniques, there are several new approaches which are increasingly being applied to bioprocess operations. Among these, of special note is expert system technology, which provides possibilities for the design of efficient bioprocess control systems with new functional capabilities. This technology has been successfully applied to variety of microbial processes at laboratory and industrial scale. The present paper analyzes the possibility for application of expert systems to animal cell cultures processes whose high complexity is well suited to expert control. The discussion focuses on the organization and the functionality of the intelligent control systems, and covers some practical aspects of their design. 相似文献
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Artificial neural network based experimental design procedure for enhancing fermentation development 总被引:1,自引:0,他引:1
Conventional experimental design techniques are available to assist in the optimization of fermentation processes, but due to the nonlinearities in the bioprocess, they are limited in their effectiveness. This problem is further complicated with recombinant systems as a result of the additional complexities of the process. This article describes a general strategy using artificial neural networks as an alternative approach to fermentation process development laboratory are presented for the neural network based procedures. (c) 1994 John Wiley & Sons, Inc. 相似文献
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Standard bioprocess conditions have been widely applied for the microbial conversion of raw material to essential industrial products. Successful metabolic engineering (ME) strategies require a comprehensive framework to manage the complexity embedded in cellular metabolism, to explore the impacts of bioprocess conditions on the cellular responses, and to deal with the uncertainty of the physiochemical parameters. We have recently developed a computational and statistical framework that is based on Metabolic Control Analysis and uses a Monte Carlo method to simulate the uncertainty in the values of the system parameters [Wang, L., Birol, I., Hatzimanikatis, V., 2004. Metabolic control analysis under uncertainty: framework development and case studies. Biophys. J. 87(6), 3750-3763]. In this work, we generalize this framework to incorporate the central cellular processes, such as cell growth, and different bioprocess conditions, such as different types of bioreactors. The framework provides the mathematical basis for the quantification of the interactions between intracellular metabolism and extracellular conditions, and it is readily applicable to the identification of optimal ME targets for the improvement of industrial processes [Wang, L., Hatzimanikatis, V., 2005. Metabolic engineering under uncertainty. II: analysis of yeast metabolism. Submitted]. 相似文献