Environmental characteristics,landscape history and pressures on three coastal lagoons in the Southern Mediterranean Region: Merja Zerga (Morocco), Ghar El Melh (Tunisia) and Lake Manzala (Egypt)

Three North African coastal lagoons were selected as primary sites for integrated ecological and hydrological monitoring and modelling as part of the MELMARINA Project (see Flower & Thompson, 2009). The three sites, Merja Zerga (13.2 km², Morocco), Ghar El Melh (35.6 km², Tunisia) and Lake Manzala (c. 700 km², Egypt), are permanent water bodies with at least one well-defined connection with the sea. This article provides an account of each lagoon’s physical characteristics and recent development including the impacts of human activities. The two sites on the Mediterranean (Ghar El Melh and Lake Manzala) are characterised by small tidally driven variations in water level whilst Merga Zerga, on the Atlantic coast, experiences large tidally induced water level variations and so contains large inter-tidal environments. All the three lagoons receive freshwater inflows from their landward margins, varying in magnitude, seasonality and ecological significance. Freshwater inflows from drains strongly influence ecological conditions within Lake Manzala. All the three lagoons have significant biodiversity interest, especially for resident and migratory birds as well as fish, and support local human populations. Each lagoon experienced significant changes during the twentieth century possibly affecting declines in biodiversity value. These largely resulted from agricultural expansion and intensification and include reclamation and hydrological modifications which have both decreased freshwater inflows due to upstream diversions (Merja Zerga and Ghar El Melh) and increased the influx of freshwater through the return of irrigation drainage (Merja Zerga and Lake Manzala). All three sites experienced nutrient enrichment due to agricultural runoff and discharge of domestic wastewater. Industrial waste discharge is a particular, but not exclusive, problem for Lake Manzala. Problems of water quantity and quality will increase through the twenty-first century with increasing demands for water while effects of climate change will enhance freshwater scarcity. Conflicts between human and environmental uses of water will increase and unless improvements in water use efficiency and wastewater treatment can be brought about wetlands including coastal lagoons are likely to suffer further loss and degradation. These problems will be compounded by sea level rise. Guest editors: J. R. Thompson & R. J. Flower Hydro-ecological Monitoring and Modelling of North African Coastal Lagoons     

Environmental influences on the qualitative and quantitative composition of phytoplankton and zooplankton in North African coastal lagoons

Within the framework of the international research project MELMARINA, seasonal dynamics of plankton communities in three North African coastal lagoons (Merja Zerga, Ghar El Melh, and Lake Manzala) were investigated. The sampling period extended from July 2003 to September 2004 with the aim of evaluating hydrological and other influences on the structure, composition and space-time development of these communities in each lagoon. Phytoplankton in Merja Zerga showed a quasi-permanent predominance of marine diatoms in the open sea station and in the marine inlet channel. Dinoflagellates were abundant in summer and early autumn in the marine inlet and extended into the central lagoon station. In Ghar El Melh, marine species (especially diatoms and dinoflagellates) dominated despite occasional winter inflows of freshwater. In Lake Manzala, freshwater species generally predominated and the planktonic communities were comparatively very diverse. Chlorophyceae contributed 39% of the total species recorded and diatoms and cyanophyceans were also common; the Dinophyceae, Euglenophyceae, Chrysophyceae and Cryptophyceae less so. Zooplankton communities in both Ghar El Melh and Merja Zerga were dominated by marine copepods. Rotifera, Copepoda, Ostracoda, and Cladocera were recorded in both lagoons as were meroplanktonic larvae of Polychaeta, Cirripedia, Mysidacea and Gastropoda and free living nematodes. Ghar El Melh was the more productive of these two lagoons with spring and early summer being the productive seasons. Zooplankton communities in Lake Manzala were generally dominated by rotifers and highest zooplankton abundances occurred in April (2003). Sampling stations near the marine inlets showed the highest diversity and the zooplankton communities showed considerable spatial variation within this large lagoon. The three lagoons represent very different water bodies contrasted strongly in terms of tidal effects and freshwater availability. Yet, there are some similarities in ecosystem structure. Space-time development of the plankton communities was similar especially in Merja Zerga and Ghar El Melh. Species abundances and specific diversities indicated that seasonal changes in salinity and nutrient concentrations were the main influential factors. Lake Manzala was the most productive lagoon and all the three sites supported toxic algal species. Relatively low plankton biomass in Merja Zerga and Ghar El Melh probably resulted from a combination of factors including highly episodic nutrient inputs, light suppression (by turbidity) and nutrient competition with benthic algae. Water quality variables were largely driven by the hydrological regime specific to each lagoon. Nutrient enrichment and, particularly for Lake Manzala, sea level rise threaten the sustainability of the planktonic ecosystems in all three lagoons. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users. Guest editors: J. R. Thompson & R. J. Flower Hydro-ecological Monitoring and Modelling of North African Coastal Lagoons     

Application of remote sensing to site characterisation and environmental change analysis of North African coastal lagoons

This article describes the use of satellite imagery for identifying key environmental characteristics within three North African coastal lagoons (Merja Zerga, Morocco; Ghar El Melh, Tunisia and Lake Manzala, Egypt) and for detecting the major environmental changes within these environments. A combination of Landsat MSS, Landsat TM, Landsat ETM+ and ASTER imagery was acquired for the three sites for a period covering the last three decades (1972–2004). Following geometric correction and enhancement, the interpretation of the most recent image acquired for each of the three lagoons provides important insights into their current conditions. For Merja Zerga, these include the distribution of the largest channels which drain extensive inter-tidal mudflats and the two major depositional features associated with sources of freshwater. The distribution of marginal aquatic vegetation is highlighted as is the intensive use of the surrounding landscape for agriculture. Intensive agriculture around Ghar El Melh is also indicated. The influence of the Mejerda River, which was diverted away from the lagoon over 100 years ago, is shown to persist as a residual area of deltaic deposits in shallow water that has been eroded over time. Coastal processes including the direction of the alongshore sediment transport and the influence of engineering work associated with port construction can also be recognised. Within Lake Manzala, vegetated islands divide the lake into a series of sub-basins which can be clearly distinguished. The large influence of human activities within this lake can be identified and include reclamation for agriculture and the conversion of parts of the lake bed for fish farms. The historical images available for the three lagoons provide important insights into decadal scale changes, which have been greatest at Lake Manzala. Since the early 1970s large parts of the lake, in particular in the southwest where the shoreline has migrated northwards, have been reclaimed. Major engineering works, such as the El Salam Canal and road embankments, are shown to have resulted in significant lake change. The distribution of emergent vegetation within the lake has also changed. Classification of images for this lake into open water, vegetation and land enables the quantification of these changes. Between 1973 and 2003, the lake declined in area by approximately 50%. Changes at Merja Zerga over the last three decades include reconfiguration of the marine outlet and the expansion of the internal delta at the end of the Nador Canal. The images of this site clearly demonstrate the intensification of agriculture around the lagoon. The most marked changes evident within the images of Ghar El Melh concern the sand bars that separate the lagoon from the sea. Geomorphological processes operating within the coastal zone have resulted in the straightening of the bars with central sections migrating out towards the sea. Remote sensing is established as a promising application for detecting the quantitative surface cover changes in coastal lagoons and their near landscapes. Guest editors: J. R. Thompson & R. J. Flower Hydro-ecological Monitoring and Modelling of North African Coastal Lagoons     

The fish fauna of three North African lagoons: specific inventories,ecological status and production

This paper examines the ecological and biological status of fisheries in three coastal lagoons in the southern Mediterranean region: Merja Zerga in Morocco, Ghar El Melh in Tunisia and Lake Manzala in Egypt. Despite similarities in some ecological characteristics, the three lagoons’ respective fisheries show differences in specific composition, in population structure and in their production both in qualitative and quantitative aspects. Thus, in Merja Zerga and Ghar El Melh the fish fauna shows a marine affinity where grey mullet and eels dominate the fish production. In Lake Manzala the ichthyofauna displays a more freshwater affinity with tilapia the dominant group of species. Otherwise, overall fish production at the three sites is regulated by variations in fishing activities, local environments and seasonal conditions. A decrease in fish production was noted over recent years and this is attributed to deteriorating ecological conditions. A variety of factors are implicated including sea communication problems, reduction of the continental (fresh) water supply and increase of pollution causing eutrophication. In addition, over fishing with a continuing increase of fishing effort units, contributes to fisheries decline. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users. Guest editors: J. R. Thompson & R. J. Flower Hydro-ecological Monitoring and Modelling of North African Coastal Lagoons     

Sediment distribution and accumulation in lagoons of the Southern Mediterranean Region (the MELMARINA Project) with special reference to environmental change and aquatic ecosystems

Surface sediments and sediment cores were collected from coastal lagoons and lakes located in the Southern Mediterranean Region (SMR) as part of the MELMARINA Project which involved integrated eco-hydrological monitoring and modelling. This study uses surface sediments and sediment cores to infer spatial characteristics and temporal changes at the MELMARINA primary sites, Merja Zerga in Morocco, Ghar El Melh in Tunisia and Lake Manzala in Egypt. In addition, surface sediment sampling was undertaken at Egyptian Lake Bardawil and sediment cores were collected from the Lagune de Nador (Morocco). Sediment distribution patterns are investigated using GIS with georeferenced sample locations to facilitate display and resurvey. Major variations in sedimentary organic matter and, particularly, carbonate content, occur within and between sites. Local landscapes combined with hydrological and biogeochemical processes influence the distributions of sediment bulk components (carbonates, organic material and clastic matter) and molluscan shells and shell debris are an important source of sedimentary carbonate at all three primary sites. Sediment cores were dated using natural (²¹⁰Pb) and artificial (¹³⁷Cs) radionuclides, and sediment accumulation rate changes indicate that sources of sediment supply varied markedly through the twentieth century but have generally diminished after the mid-1960s. Sedimentary siliceous microfossils (diatoms) were generally poorly preserved, but mollusc shell remains were well represented. Sediment chronologies and sediment bulk composition allow discussion of some recent changes in bulk, minerogenic and biogenic sediment accumulation patterns in the SMR lagoons. Sediment accumulation rates also varied between sites and multiple cores from Lake Manzala indicated that rates showed considerable spatial variability. Low-level sediment contamination by fossil fuel combustion particulates and trace metals was demonstrated for Ghar El Melh and Lagune de Nador where Pb and Zn accumulation rates were highest in twentieth century sediment. It is emphasized that sediment quality and quantity have strong influences on lagoon ecosystem function and sedimentation is relevant to hydromorphology and to concepts of ecological quality. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users. Guest editors: J. R. Thompson & R. J. Flower Hydro-ecological Monitoring and Modelling of North African Coastal Lagoons     

Spatio-temporal dynamics of the nematode Anguillicola crassus in Northeast Tunisian lagoons

Anguillicola crassus, parasite nematode of the European eel Anguilla anguilla, was recorded for the first time in Tunisia (1999) in the Ichkeul lagoon. Its distribution has since spread toward Bizerte and Ghar El Melh lagoons. The monthly epidemiological survey reveals that A. crassus exists throughout the year in the Ichkeul lagoon. In this lagoon, its prevalence is low in winter (12% in December), with a marked increases in the spring reaching a maximum in March (35%), before it starts to decrease in summer with a minimum in July (4.35%), which in turn is followed by a pronounced new rise in autumn (30% in November). However, mean intensity values do not show such a marked variation. The majority of the values are between 1 and 1.5 parasites per host. In the Bizerte and Ghar El Melh lagoons, the presence of this nematode is limited only to one to three months. Investigations in the Tunis lagoon did not reveal until now the presence of A. crassus. It has been observed that the length of the eel influences the prevalence values: A. crassus becomes less common if the length of the eel increases. Comparatively with the global epidemiological values of A. crassus signalled subsequently (1999) in the Ichkeul lagoon, we note that the present values record a clean increase.     

Influence of environmental conditions on the distribution of Amphipoda,Talitridae, in the lagoon complex of Ghar El Melh (north‐east of Tunisia)

The study of the spatiotemporal distribution of talitrid amphipods was carried out along a transect in three stations belonging to the lagoon complex of Ghar El Melh, namely the supralittoral zones of the old harbour of Ghar El Melh, the opposite to Boughaz and Sidi Ali Mekki lagoon. Four species belonging to Talitridae family with two different genera were identified at all stations. These species are Orchestia gammarellus, Orchestia mediterranea, Orchestia stephenseni and Platorchestia platensis. The first two species were collected during the four seasons. The study of the density showed that the most important global mean density in talitrid was found in the supralittoral zone of opposite to Boughaz. In addition, statistical analysis showed a significant difference in the seasonal distribution of amphipods based on plant associations. Furthermore, the highest diversity of talitrid was observed in the winter in the supralittoral zone of opposite to Boughaz and in summer in the supralittoral zones of Sidi Ali Mekki lagoon and the old harbour of Ghar El Melh. Results showed that at all stations, a highly significant correlation of amphipod community with Cymodocea banquette was observed. Moreover, principal component analysis showed that the distribution of the different species depends on climatic and edaphic factors.     

Environmental science and management of coastal lagoons in the Southern Mediterranean Region: key issues revealed by the MELMARINA Project

Lagoons in the heavily populated, semi-arid coastal zone of the Southern Mediterranean Region exemplify the conflict between human utilisation of water and related resources and aquatic ecosystems. Having recognised the requirement to improve understanding of the functioning of the region’s coastal wetlands, the MELMARINA Project undertook integrated hydro-ecological monitoring and modelling within lagoons in Morocco, Tunisia and Egypt. This article highlights some key issues regarding environmental science and management of the region’s coastal lagoons revealed during the course of the project. It stresses the importance of hydrology as a key control upon lagoon functioning and ecosystem dynamics. Hydrological modifications due to water resource management schemes are the cause of many recent changes experienced within lagoons. Linkages between water quality, water availability, human activities and biological characteristics of coastal lagoons are discussed with particular reference to the controls upon vegetation within the MELMARINA lagoons. A series of methodological advances are reviewed which have potential for wider application within coastal lagoons. It is suggested that the use of lagoon sediment for environmental reconstruction can be invaluable, especially when monitoring data are lacking. Recent advances in instrumentation technologies make long-term continuous monitoring more feasible although these approaches can be combined with more traditional site surveys to provide wider spatial coverage at the expense of temporal resolution. Wider spatial coverage can also be achieved through the use of space-borne or aerial remote sensing imagery whilst longer-term trends in site characteristics can be assessed through historical map analyses. Geographical Information Systems, which facilitate the storage and interrogation of large and varied datasets, have enormous potential. Similarly, coupled hydro-ecological models can inform understanding of lagoon functioning and can assess scenarios associated with environmental change or alternative management approaches. The application of integrated, basin-wide approaches to the management of water resources and aquatic ecosystems in the Southern Mediterranean Region is advocated. This includes the application of principles from the EU’s Water Framework Directive. Finally, the need to place management in the context of climate change and associated sea level rise is stressed. Emphasis should be placed on the development of adaptation strategies designed to minimise the effects of these changes. Guest editors: J. R. Thompson & R. J. Flower Hydro-ecological Monitoring and Modelling of North African Coastal Lagoons     

Characterisation of a Tunisian coastal lagoon through hyperspectral underwater irradiance

North African coastal lagoons are unique ecosystems that often suffer degradation due to human activities. Therefore, monitoring methods are required to identify stressors and assist with the management of these valuable and often understudied ecosystems. A synthetic indicator of water ecological quality would be desirable for regular monitoring of these ecosystems under pressure. In 2008 an optical procedure was developed and applied in Ghar El Melh, a Tunisian lagoon which has been increasingly impacted by pollutant loading, especially from agriculture. In situ hyperspectral irradiance was measured at several stations, from which the apparent optical properties (AOPs), namely the irradiance attenuation coefficient K(λ) and the reflectance ratio R(λ), were obtained in order to relate them to water composition, in terms of light-attenuating substances (LASs). The significant relationships observed between R and LAS values enabled the application of a hyperspectral optical classification, which effectively highlighted threatened sectors of the lagoon. The pattern of differing water quality across the lagoon system that was derived from the hyperspectral classification agreed well with that obtained from a conventional optical classification that included AOPs and LASs. We suggest that hyperspectral analysis and classification is a useful monitoring tool for the assessment of change in coastal lagoons, and perhaps also in other shallow-water ecosystems.     

Seasonal rather than spatial variability drives planktonic and benthic bacterial diversity in a microtidal lagoon and the adjacent open sea

Coastal lagoons are highly productive ecosystems, which are experiencing a variety of human disturbances at increasing frequency. Bacteria are key ecological players within lagoons, yet little is known about the magnitude, patterns and drivers of diversity in these transitional environments. We carried out a seasonal study in the Venice Lagoon (Italy) and the adjacent sea, to simultaneously explore diversity patterns in different domains (pelagic, benthic) and their spatio‐temporal variability, and test the role of environmental gradients in structuring assemblages. Community composition differed between lagoon and open sea, and between domains. The dominant phyla varied temporally, with varying trends for the two domains, suggesting different environmental constraints on the assemblages. The percentage of freshwater taxa within the lagoon increased during higher river run‐off, pointing at the lagoon as a dynamic mosaic of microbial taxa that generate the metacommunity across the whole hydrological continuum. Seasonality was more important than spatial variability in shaping assemblages. Network analyses indicated more interactions between several genera and environmental variables in the open sea than the lagoon. Our study provides evidences for a temporally dynamic nature of bacterial assemblages in lagoons and suggests that an interplay of seasonally influenced environmental drivers shape assemblages in these vulnerable ecosystems.     

Optical characterization of coastal lagoons in Tunisia: Ecological assessment to underpin conservation

Ghar El Melh is a shallow lagoon (average depth of 0.8 m) that has undergone a eutrophication process due to growing human pressures. To obtain a global frame of the ecosystem functioning, an optical and an ecological classification were used in parallel. Downwelling and upwelling spectral irradiances were measured in situ in 22 sampling stations across the water body; then Apparent Optical Properties (AOPs), namely reflectance R(λ) and vertical attenuation coefficient K_d(λ) were calculated for each wavelength of visible spectrum, furnishing typical spectra from turbid waters, rich in dissolved and suspended matter. From water samples of the same stations the concentrations of OASs (Optically Active Substances), i.e. Chromophoric Dissolved Organic Matter (CDOM), Non-Algal Particulate (NAP) and Phytoplankton, were assessed. The use of an optical classification for water bodies rich in TSM and CDOM, integrating AOPs and OASs, highlighted a great spatial heterogeneity, well overlapping with hydrology and human impacts patterns. A modified version of the Ecological Evaluation Index (EEI), considering the macrophyte distribution (based on a visual assessment of macrophyte coverage, without quantitative sampling) was then used, highlighting an intermediate ecological condition, despite high water turbidities. The integrated use of both systems thus furnished a complete characterization, rapidly detecting the most impacted sectors and the possible primary causes. The method might be applied as a monitoring procedure in other Mediterranean coastal lagoons, with the aim to adopt a common conservation strategy for these important transitional water bodies.     

Structure and bioassessment of benthic communities of a lagoonal ecosystem of the Atlantic Moroccan coast

The Merja Zerga lagoon is a semi-enclosed marine ecosystem in which various types of human activities have been developed. This paper characterizes the biosedimentary units of the lagoon and defines a reference status of the quality and health of the macrozoobenthic communities that can be used as bioindicators of the quality of the global marine environment. Specific and functional diversity were high: 147 taxa were identified; they were distributed within seven main trophic groups. Trophic structure is dominated by the suspension-feeding bivalve Cerastoderma edule and the deposit-feeding bivalve Scrobicularia plana, while micrograzers and macroherbivores remain low. Biotic index values indicated that the site is moderately perturbed and that the benthic communities are unbalanced. Nevertheless, the communities showed a seasonal stability of abundances and a high specific richness all through the year.     

Open water zooplankton communities in North African wetland lakes: the CASSARINA Project

Zooplankton (Copepoda, Cladocera, Ostracoda, Rotifera and Diptera larvae) in nine North African lakes was collected from open water areas over twenty months during 1997/99. The results were used to monitor changes in the pelagic micro-invertebrate fauna of these sites with the purpose of exploring diversity structure and regional species occurrences.The studied sites formed three distinct groups based on hydrology and water quality criteria: (i) acid water with no marine connection (Megene Chitane); (ii) alkaline freshwater/brackish with no marine connection (Merja Sidi Bou Rhaba and Merja Bokka); (iii) freshwater/brackish with marine connection (Merja Zerga, Lac de Korba, Garaet El Ichkeul and three Nile Delta lakes). However, cluster analysis of the zooplankton data alone indicated four groups with Korba being separated because of its prevalence of species tolerant of summer hypersalinity.The total regional zooplanktonic species richness found was 88 taxa and these were characterized by species tolerant of widely fluctuating environmental conditions. However, some recorded species were very rare for North African freshwaters (e.g. Alonella excisa, Leydigia quadrangularis and, Ilyocryptus sordidus) and generally indicate favourable environmental conditions of low salinity and temperature. The sites influenced by marine waters generally exhibited slightly lower numbers of species but which generally demonstrate cosmopolitan distributions. Distinct seasonal patterns in species distributions were more similar to those observed in European lakes rather than to those of lower latitudes sites.Zooplankton play a key role in maintaining aquatic ecosystem quality in the North African study lakes and the community distributions described for the late 20th century help set biodiversity base-line data for future studies. If the remaining wetland lakes in this region are to persist as important resources during the 21st century, they will need to be managed in a way that ensures that aquatic diversity is maintained.     

Spatiotemporal Variation in Shallow-Water Freshwater Fish Distribution and Abundance in a Large Subtropical Coastal Lagoon

Patos Lagoon is located off the southern Brazilian coast and represents one of the largest coastal lagoons in the world. We estimated hydrological and physicochemical conditions associated with spatial variation in the abundance and diversity of freshwater fishes along the lagoon, and inter-annual variability in abundances of freshwater fishes occurring in its estuarine zone. During our study, the region experienced two periods of average rainfall and two periods with above-average rainfall. The characids Astyanax eigenmaniorum and Oligosarcus jenynsii and the siluriform Parapimelodus nigribarbis were the most abundant freshwater fishes in the estuary during wet periods when water levels were higher and salinity was lower. Increases in abundance of these species in the estuarine area, all of which members of primary-division freshwater families, apparently were associated with pulses of reproduction and passive transport from freshwater habitats located near middle and upper lagoon reaches. Abundance of species from secondary freshwater families, such as poeciliids and cichlids, were less correlated with hydrological conditions, and their patterns of occurrence in the estuary suggest active migration from nearby freshwater habitats draining into this area. Findings indicate that freshwater discharge in the basin and expansion/retraction of freshwaters in the middle-upper lagoon determined patterns of freshwater fish abundance and species richness in the estuarine zone.     

North African wetland lakes: characterization of nine sites included in the CASSARINA Project

Exploitation of land and water resources has increased rapidly in North Africa during the 20th century, paralleling regional population growth. As part of the CASSARINA Project (see Flower, 2001), the environmental status of nine wetland lakes in Morocco, Tunisia, and Egypt was evaluated. All are conservationally important habitats and several are Ramsar Sites (internationally recognized bird reserves) and several support significant fisheries. All are shallow (<2 m in depth) but vary greatly in area.Where available, documentary information on relevant 20th century changes is given. Survey transects for aquatic vegetation were established and used to provide baseline ecological information on the aquatic plant communities during 1997–1999. Unusually, one site (Tunisian Megene Chitane) supported acidophilous vegetation (some taxa being nationally rare). Aquatic macrophytes declined catastrophically at two sites during the 1990s. Merja Bokka was drained in 1998 and, at Garaet El Ichkeul, fringing Phragmites and Scirpus spp. were lost, mainly as a result of salinity changes. Elsewhere, fringing macrophytes remain (extensively so in the Nile Delta lakes) common, despite major land reclamation and water quality problems, or are degraded by grazing (Merja Zerga). Marginal vegetation during 1997/98 changed markedly at Megene Chitane due to water level lowering.Documentary records indicated that throughout the 20th century, reclamation and hydrologic modifications, mainly for agricultural purposes, affected all nine sites. The loss of lake area by reclamation is substantial for the Nile Delta lakes (Edku, Burullus and Manzala). For the western sites, some data indicate increasing salinity in the most recent decade but the Delta lakes have become generally fresher during the 20th century, as supply of Nile water for irrigation increased.Despite intense human disturbance, many of the remaining CASSARINA sites still support regions of high aquatic diversity. Spatial scale monitoring of the larger sites for seasonal and inter-annual changes in open water area and in aquatic plant abundances is a key requirement for integrated environmental change assessment in the 21st century.     

Heterotrophic bacteria associated with the green alga <Emphasis Type="Italic">Ulva rigida</Emphasis>: identification and antimicrobial potential

Heterotrophic bacteria associated with the green alga Ulva rigida, collected from the coast of Tunisia, were isolated and subsequently identified by their 16S rRNA gene sequences and by phylogenetic analysis. The 71 isolates belong to four phyla: Proteobacteria (Alpha-and Gamma- subclasses), Bacteroidetes, Firmicutes, and Actinobacteria. Most of the isolates belong to Proteobacteria. The Gram-positive Firmicutes and especially the genus Bacillus were well-represented at the surface of U. rigida, collected from the coast as well as from the lagoon, while Actinobacteria were represented only at the surface of algae collected from the coast of Cap Zebib. Bacteroidetes were more represented at the surface of algae collected from the Ghar El Melh lagoon. The bacterial community of the water surrounding the algae was different from that associated with the surface of the algae. Moreover, the abundance of bacteria in the surrounding water was much lower compared to the density of bacteria associated with the surface of the algae. Bacteria isolated from the algal surface were tested for their antimicrobial potential. The results show that ~?36% of the algae-associated bacterial isolates possess antibacterial activity whereas free-living bacteria, isolated from the surrounding water, did not show such activity. The surface of U. rigida was colonized by a high diversity of culturable and possibly novel epiphytic bacteria that may be an important source of antimicrobial compounds and are therefore of biotechnological interest.     

An overview of integrated hydro-ecological studies in the MELMARINA Project: monitoring and modelling coastal lagoons—making management tools for aquatic resources in North Africa

As landscape disturbance and climate conspire to accelerate global environmental change towards unprecedented levels in the twenty-first century, the populated coastal regions of many countries are facing major threats to sustainability. Coastal water resources are particularly vulnerable in dry regions. In view of the expected severity of future environmental change in the Southern Mediterranean Region, the European Commission supported an integrated multidisciplinary project, MELMARINA, on monitoring and modelling coastal lagoons in Morocco, Tunisia and Egypt. This is a region where water management for people and for agriculture has been intense particularly during the twentieth century, yet long-term environmental monitoring and management of wetland ecosystems are under developed. Not only are biodiversity aspects at risk in coastal lagoons and wetlands but the goods and services that affect human welfare are also generally in decline. Co-ordinated hydro-ecological monitoring at key wetland lagoons was begun in 2003 with a view to establishing environmental baselines and calibrating site-specified hydro-ecological models. This article introduces the project and its results that range from lagoon typification and hydro-ecology to the application of hydro-ecological models. Detailed results and evaluations are presented in a linked series of themed scientific articles within this special issue. The present condition of the lagoons investigated essentially results from various hydrological modifications combined with eutrophication problems, yet all still remain valuable aquatic ecosystems. Adequate monitoring data are an essential part of reliable predictive modelling and, despite several data gaps, nutrient load reduction scenarios were undertaken to help target restoration aims. Implementation of aspects of the EU Water Framework Directive for achieving good ecological status of transitional waters is advocated. Nevertheless, as the twenty-first century advances the effects of global climate change are expected to amplify current stresses making intervention restoration and adaptation management even more imperative. Long-term sustainability depends upon detecting and measuring environmental change (long-term water quality and ecological quality) and incorporating the results into appropriate hydro-ecological models to facilitate the development of appropriate management initiatives. Guest editors: J. R. Thompson & R. J. Flower Hydro-ecological Monitoring and Modelling of North African Coastal Lagoons     

Diversity of terrestrial isopods in the supralittoral zone of Ghar El Melh lagoon (Tunisia)

In Tunisia, while wetlands are considered as remarkable habitats for their faunal and floral diversity, few studies on the species diversity of terrestrial isopods were performed. To fill this gap, the spatio‐temporal distribution of Oniscidea at Ghar El Melh lagoon (north‐east of Tunisia) was analysed. Sampling was carried out with 50 × 50 cm quadrats along a perpendicular transect to the shoreline. Nine species of terrestrial isopods belonging to seven genera were identified. Chaetophiloscia elongata Dollfus, 1884 was the most abundant species in all seasons except for summer; its relative abundance ranged from 38.9% to 77% in summer and autumn, respectively. Hill diversity indices ranged from 0.86 to 0.94 and equitability indices from 0.33 and 0.68. The statistical analysis showed a significant difference of isopod species and plant associations among seasons.     

Effect of age,sex, and mutual interaction on the locomotor behavior of Orchestia gammarellus in the supralittoral zone of Ghar El Melh lagoon (Bizerte,Tunisia)

Freshly collected individuals of Orchestia gammarellus from the supralittoral zone of Ghar El Melh lagoon (Northern of Tunisia) were housed in spring in a controlled-environment cabinet. Locomotor activity rhythm of this species was recorded in spring, at a constant temperature of 18 ± 0.5 °C. In the first experiment, juveniles and adults specimens were kept under light-dark cycle in phase with the natural diel cycle. In the two other experiments (males/females and mixed/unmixed groups), individuals of O. gammarellus were maintained under constant darkness. According to double-plotted actograms, waveforms and periodogram analysis, results revealed different locomotor pattern. However, locomotor rhythm of juveniles was more stable than adults. Also, the locomotor activity rhythm of groups was more defined than that of the isolated individuals. Moreover, the activity of mixed groups as well as unmixed groups was more pronounced than the activity of the isolated individuals. Furthermore, results showed no significant difference between locomotor rhythm pattern of males and females of this species.