共查询到20条相似文献,搜索用时 46 毫秒
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
Csenge Gal Karen M Moore Konrad Paszkiewicz Nicholas A Kent Simon K Whitehall 《Cell cycle (Georgetown, Tex.)》2015,14(1):123-134
HIRA is an evolutionarily conserved histone chaperone that mediates
replication-independent nucleosome assembly and is important for a variety of processes
such as cell cycle progression, development, and senescence. Here we have used a chromatin
sequencing approach to determine the genome-wide contribution of HIRA to nucleosome
organization in Schizosaccharomyces pombe. Cells lacking HIRA experience
a global reduction in nucleosome occupancy at gene sequences, consistent with the proposed
role for HIRA in chromatin reassembly behind elongating RNA polymerase II. In addition, we
find that at its target promoters, HIRA commonly maintains the full occupancy of the
−1 nucleosome. HIRA does not affect global chromatin structure at replication
origins or in rDNA repeats but is required for nucleosome occupancy in silent regions of
the genome. Nucleosome organization associated with the heterochromatic
(dg-dh) repeats located at the centromere is perturbed by loss of HIRA
function and furthermore HIRA is required for normal nucleosome occupancy at Tf2 LTR
retrotransposons. Overall, our data indicate that HIRA plays an important role in
maintaining nucleosome architecture at both euchromatic and heterochromatic loci. 相似文献
12.
13.
14.
15.
16.
17.
18.
19.