Effect of catecholamines on bursa of fabricius in chicken

Effect of catecholamines are studied on the bursa of fabricius of chicken. It is found that in epinephrine (E) treated chicken, the lymph follicles are slightly decreased in size. Some amount of nuclear pycnosis is visible in E and norepinephrine (NE) treated chicken. There is no change in the bursa weight and histology in NE treated groups. No deviation is observed in the level of DNA, RNA, total protein and sialic acid content of catecholamine treated birds.     

Bursopentin (BP5) from chicken bursa of fabricius attenuates the immune function of dendritic cells

Bursopentine (BP5), a novel pentapeptide isolated from chicken bursa of fabricius, has been proved to have immunomodulatory effects on B and T lymphocytes, anti-oxidative stress on macrophages, and antiproliferation on tumor cells. However, the effects of BP5 on the immune function exhibited by dendritic cells (DCs), which are regarded as a major target for immunomodulators, remain unknown. In this study, we examined the effects of BP5 on the activation and maturation of murine bone marrow-derived DCs. Our results showed that BP5 significantly suppressed the secretion of lipopolysaccharide (LPS)-induced pro-inflammatory (TNF-α, IL-1β, IL-6 and IL-12p70) and anti-inflammatory (IL-10) cytokines by DCs, and this impact was not due to its cytotoxicity. Besides, BP5 reversed the morphological changes and attenuated the expression of phenotypic markers (MHC II, CD40, CD80 and CD86 molecules) in LPS-induced DCs. Furthermore, BP5 restored the decreased FITC-dextran uptake in LPS-treated DCs, arrested the LPS-induced migration of DCs and abrogated the promoting ability of LPS-induced DCs for allogeneic T cell proliferation. These findings show a new immunopharmacological capability of BP5 and provide a novel approach in the prevention and therapy of chronic inflammation and autoimmunity via abolishing the immune function of DCs.     

Comparative study of the glycosphingolipids of chicken bursa of Fabricius and of chicken, rat and human thymus.

1. The glycosphingolipid compositions of the thymus and bursa of Fabricius of young male chickens were compared. The two tissues were found to contain complex mixtures of both neutral glycosphingolipids and gangliosides. Both tissues contained mono-, di-, tri-, tetra- and penta-glycosylceramides; the pentaglycosylceramide displayed a reaction of identity with authentic Forssman antigen when tested against a specific anti-(Forssman antigen) serum. The ganglioside G(m3) containing N-acetylneuraminic acid was the principle ganglioside of both tissues. 2. The thymus contained appreciable amounts of the simple ganglioside N-acetylneuraminylgalactosylceramide, a compound not found in the bursa. The ganglioside G(d3) (disialohaematoside) was detected in both tissues. 3. Rat and human thymus, like sheep thymus (Narasimhan, Hay, Greaves & Murray (1976) Biochim, Biophys. Acta 431, 578-591), both contained a tetraglycosylceramide species as their most complex neutral glycosphingolipid and possessed little or no Forssman antigen. They also contained a complex mixture of gangliosides. 4. The possible significance of these results is briefly discussed.     

鸡法氏囊B淋巴细胞中与鸡传染性法氏囊病病毒VP2相互作用蛋白筛选

为了获得鸡法氏囊B淋巴细胞中与鸡传染性法氏囊病病毒 (IBDV) VP2相互作用的蛋白质,利用酵母双杂交系统,用IBDV VP2蛋白为诱饵蛋白,筛选鸡法氏囊B淋巴细胞cDNA表达文库。将表达文库质粒转化含IBDV VP2诱饵质粒的酵母感受态细胞,检测报告基因在相应的营养缺陷型培养基 (SD/-Leu/-Trp/-His) 上表达情况,进一步经β-半乳糖苷酶报告基因检测,筛选到16个阳性克隆。提取阳性克隆质粒,经测序分析获得5个原鸡基因序列,分别是：线粒体DNA、蛋白质O位N-乙酰葡萄糖胺糖基化转移酶、肿瘤     

Immunoreactive neurotensin and somatostatin in the chicken thymus

Summary Two distinct populations of endocrine cells in the chicken thymus display neurotensin and somatostatin immunoreactivity, respectively. Both cell types are few in number at hatching but proliferate rapidly during the first week. The neurotensin cells are Grimelius-positive and Hellerström-Hellmannegative. The somatostatin cells are Grimelius-negative and Hellerström-Hellman-positive. Both cell populations are non-argentaffin. The somatostatin-like material extracted from chicken thymus behaves immunochemically and chromatographically similar to synthetovine somatostatin, while the neurotensin-like material, from the thymus as well as from the gut, differs from synthetic bovine neurotensin in that it appears larger in size and more basic.     

Sonic hedgehog在鸡胚法氏囊发育过程中的表达研究

Sonic hedgehog(Shh)是Hedgehog(Hh)家族中的一员,在胚胎发育和器官形成过程中发挥重要作用.法氏囊是鸟类所特有的中枢免疫器官,在机体的免疫防御方面发挥重要作用.利用切片原位杂交的方法探究Shh基因在鸡胚法氏囊发育过程中的表达模式,检测发现Shh基因主要在鸡胚法氏囊的囊下上皮细胞、血管周围上皮细胞以及网状细胞中表达.     

Regulating properties of peptides of the thymus and bursa of Fabricius in immunodepression in birds

The intensity of nucleic acids and protein synthesis in the cells of the thymus and the bursa of Fabricius was studied in chickens against the background of an immunodepression induced by administration of hydrocortisone and cyclophosphamide. It was found out that hydrocortisone causes in chicken a marked lowering of the intensity of inclusion of 3H-thymidine, 3H-uridine and 14C-glycine in thymic cells, and cyclophosphamide--in the cells of the bursa of Fabricius. Under the conditions of selective immunodepression the preparations on the basis of the peptides of the thymus (thymalin) and of the bursa of Fabricius (bursilin) regulate the processes of nucleic acids and protein synthesis chiefly in the cells of organs which produce them.