双歧杆菌DNA对巨噬细胞MAPK的影响

目的 探索青春型双歧杆菌的DNA对巨噬细胞丝裂素活化的蛋白激酶（MAPK）活性的影响。方法 以激光共聚焦显微镜定量测定小鼠腹腔巨噬细胞MAPK家系中ERK1／2、JNK和p38的含量。结果 双歧杆菌DNA注射组小鼠腹腔巨噬细胞ERK1／2的平均荧光强度明显高于对照组（P〈0．01）,而JNK和p38的平均荧光强度在2组间则差异无显著性（P〉0．05）。结论 青春型双歧杆菌的DNA能提高巨噬细胞ERK1／2的活性,这可能是其激活巨噬细胞的途径之一。     

双歧杆菌DNA对巨噬细胞PKC的影响

目的探索青春型双歧杆菌的DNA对巨噬细胞PKC家族的影响.方法以激光共聚焦显微镜定量测定小鼠腹腔巨噬细胞PKCα、PKCβⅠ、PKCβⅡ、PKCγ、PKCε和PKCζ的含量.结果双歧杆菌DNA注射组小鼠腹腔巨噬细胞PKCα和PKCβⅡ的平均荧光强度明显高于对照组（P＜0.01）,而PKCβⅠ、PKCγ、PKCε和PKCζ的平均荧光强度在2组间则差异无显著性（P＞0.05）.结论青春型双歧杆菌的DNA能活化巨噬细胞的PKCα和PKCβⅡ.     

双歧杆菌习腹腔巨噬细胞激活作用的初步观察

用青春型双歧杆菌注射于裸鼠腹腔,分别以中性红吞噬法以及ＭＴＴ法检测了裸鼠腹腔巨噬细胞的吞噬能力和能量代谢水平。结果显示双歧杆菌注射组裸鼠腹腔巨噬细胞的吞噬能力和能量代谢水平均显著高于对照组（Ｐ＜０．０１）。提示青春型双歧杆菌能激活巨噬细胞,增强其吞噬功能,提高其能量代谢水平。     

双歧杆菌对裸鼠腹腔巨噬细胞激活作用的初步观察

用青春型双歧杆菌注射于裸鼠腹腔,分别以中性红吞噬法以及M TT 法检测了裸鼠腹腔巨噬细胞的吞噬能力和能量代谢水平。结果显示双歧杆菌注射组裸鼠腹腔巨噬细胞的吞噬能力和能量代谢水平均显著高于对照组(P< 0.01)。提示青春型双歧杆菌能激活巨噬细胞,增强其吞噬功能,提高其能量代谢水平     

双歧杆菌对裸鼠腹腔巨噬细胞NO形成的调节作用

给裸小鼠腹腔注射青春型双歧杆菌,每天一次,连续５天,以Ｇｒｉｅｓ试剂测定了裸鼠腹腔巨噬细胞分泌ＮＯ的含量。结果表明：双歧杆菌注射组其腹腔巨噬细胞产生ＮＯ的量显著高于对照组,具有显著的统计学意义（Ｐ＜００１）。提示青春型双歧杆菌可激活巨噬细胞,使之产生一定量的ＮＯ,ＮＯ在介导双歧杆菌的多种生理功能方面起重要作用     

双歧杆菌的完整肽聚糖对巨噬细胞NF-kB的影响

目的从NF-kb角度探索分叉双歧杆菌的完整肽聚糖（WPG）激活巨噬细胞的信号机制.方法首先分离培养SD大鼠腹腔巨噬细胞,然后以不同浓度的WPG刺激巨噬细胞,最后采用凝胶电泳迁移分析技术检测巨噬细胞NF-kb的DNA结合活性.结果分叉双歧杆菌的WPG作用于巨噬细胞后,其NF-kB的DNA结合活性明显高于对照组（P＜0.01）.结论分叉双歧杆菌的WPG能活化巨噬细胞的NF-kb.     

青春型双歧杆菌生态制品对小鼠肝癌抑制作用的初步研究

本文观察了青春型双歧杆菌(Bif.a)对小鼠肝癌移植瘤的抑制作用。结果发现,青春型双歧杆菌在瘤细胞移植前或移植后应用均显示了抑制肿瘤生长的作用。将青春型双歧杆菌注入肤腔可激活肤腔巨噬细胞,提高其吞噬功能和非特异性酯酶活性,而加入体外培养的小鼠肝癌细胞未显示有杀伤瘤细胞作用。认为青春型双歧杆菌的抑瘤作用可能是该菌刺激了宿主的免疫活性细胞杀伤了瘤细胞,而非直接杀伤作用。     

胃动素对胃窦平滑肌细胞内Ca2+浓度的影响及机制

目的：观察胃动素对新生大鼠胃窦平滑肌细胞内Ca^2＋浓度的影响及机制。方法：采用激光共聚焦显微镜结合细胞内荧光染色观察不同条件下相同浓度胃动素对培养大鼠胃窦平滑肌细胞内Ca^2＋浓度的影响。结果：细胞膜Ca^2＋通道阻断剂维拉帕米、含Ca^2＋螯合剂EGTA的D-Hank’s液及细胞内Ca^2＋释放阻断剂TMB-8均可不同程度抑制MTL对胃窦平滑肌细胞内Ca^2＋的升高作用。结论：MTL具有升高胃窦平滑肌细胞内Ca^2＋的作用,细胞外Ca^2＋内流和内Ca^2＋释放参与了这种作用。     

16SrRNA荧光定量PCR法检测双歧杆菌

目的应用16SrRNA序列设计引物定量测定双歧杆菌。方法应用青春型双歧杆菌2627号作常规PCR扩增出的模板经系列稀释做荧光定量PCR,制作标准曲线;对青春型、长型、短型、婴儿型、两歧型、链型等6个种共15株双歧杆菌和大肠杆菌等10株其他细菌做荧光定量PCR,计算机通过与标准曲线比较给出定量结果;对青春型双歧杆菌2627号进行敏感性测定。结果15株双歧杆菌(10     

双歧杆菌对小鼠肝癌腹水瘤细胞Ca^2＋—ATPase及糖原磷酸化酶a的影响

采用青春型双歧杆菌菌液与小鼠肝癌腹水瘤细胞HCa/16A3-F相互作用,测定其对细胞糖原磷酸化酶a和微粒体Ca^2 -ATPase活性的影响,发现两酶的活性均明显低于对照组,这与细胞内其他一些钙稳态指标的变化相一致。提示双歧杆菌菌液对此腹水瘤钙稳态具有一定的影响,可为双歧杆菌的抗肿瘤作用提供一定的依据。     

血管形成在双歧杆菌预防大肠癌生长中的作用

目的 从血管形成角度探索青春型双歧杆菌预防大肠癌生长的途径。方法 建立大肠癌裸鼠移植瘤模型,以免疫组化法检测大肠癌组织血管内皮生长因子（VEGF）的蛋白表达水平及其微血管密度（MVD）。结果 双歧杆菌预防组大肠癌VEGF的阳性细胞密度及MVD的数量均明显低于肿瘤对照组（P〈0．01）。结论 青春型双歧杆菌能下调大肠癌VEGF的表达,进而抑制其血管形成,这可能是它预防大肠癌生长的途径之一。     

双歧杆菌的完整肽聚糖对小鼠腹腔巨噬细胞细胞骨架的影响

目的探讨双歧双歧杆菌的完整肽聚糖（WPG）对巨噬细胞细胞骨架的影响。方法首先分离培养昆明小鼠腹腔巨噬细胞,然后以WPG刺激巨噬细胞后,用荧光标记的鬼笔环肽染液染色,最后采用激光共聚焦显微镜技术检测巨噬细胞的细胞骨架。结果和对照组相比,WPG刺激巨噬细胞后,其胞内肌动蛋白减少且排列更加紊乱,同时胞膜荧光强度减弱,胞外放射状荧光物质减少,甚至消失。结论双歧双歧杆菌的WPG在激活巨噬细胞的过程中可影响其细胞骨架。     

Effect of Extracellular Calmodulin on the Cytosolic Ca2+ Concentration in Lily Pollen Grains

Using Lilium davidii Duchartre pollen as material, the calcium ion-fluorescence indicator fluo-3AM was loaded successfully into the pollen grains by low temperature loading method. Laser confocal scanning microscopy was used to study the effect of extracellular calmodulin on intracellular calcium. It is found that the purified exogenous calmodulin could elevate the intracellular calcium ion concentration, and the effect was correlated with the concentration of exogenous calmodulin to a certain extent. Cell membrane nonpermeable inhibitor of calmodulin, W 7-agarose, and the anti-serum of calmodulin could decrease the cytosolic calcium level. The results show that the endogenous extracellular calmodulin may play an important role in maintaining and increasing the cytosolic calcium level in pollen grain cell.     

双歧杆菌的完整肽聚糖对小鼠腹腔巨噬细胞缝隙链接的影响

目的探讨双歧杆菌的完整肽聚糖（WPG）对巨噬细胞缝隙连接介导的细胞间通讯（GJIC）的影响。方法首先分离培养昆明小鼠腹腔巨噬细胞,然后以WPG刺激巨噬细胞,再用脂荧光探针标记细胞,最后采用激光共聚焦显微镜结合激光漂白后荧光恢复技术检测反映GJIC变化的荧光恢复程度。结果和对照组相比,以WPG刺激巨噬细胞后,其GJIC的平均荧光恢复率明显增加（P〈0.01）。结论双歧杆菌的WPG可提高巨噬细胞的缝隙连接介导的细胞间通讯。     

双歧杆菌的完整肽聚糖对小鼠腹腔巨噬细胞膜脂流动性的影响

目的探讨分叉双歧杆菌的完整肽聚糖（WPG）对巨噬细胞膜脂流动性的影响。方法首先分离培养昆明小鼠腹腔巨噬细胞,然后以WPG刺激巨噬细胞,再用细胞膜磷脂荧光探针标记细胞,最后采用激光共聚焦显微镜结合激光漂白后荧光恢复技术检测巨噬细胞的膜脂流动性。结果WPG刺激组反映小鼠腹腔巨噬细胞膜脂流动性的平均荧光恢复率明显高于对照组（P〈0.01）。结论分叉双歧杆菌的完整肽聚糖可提高巨噬细胞膜脂流动性。     

双歧杆菌预防大肠癌生长的凋亡途径

目的探索青春型双歧杆菌预防大肠癌生长有凋亡的途径。方法首先建立大肠癌裸鼠移植瘤动物模型,然后以免疫组化法检测大肠癌裸鼠移植瘤组织caspase-3基因的蛋白表达水平。结果双歧杆菌预防组大肠癌Caspase-3的阳性细胞密度以及表达率明显高于肿瘤对照组（P〈0.01）。结论青春型双歧杆菌可通过上调caspase-3基因的蛋白表达来预防大肠癌的生长。     

Conditions for initiation of fertilization of eggs in the copulating elkhorn sculpin

The reason for failure to initiate fertilization internally was examined in a cottid fish, the elkhorn sculpin, Alcichthys alcicornis which has internal gametic association and external fertilization. While eggs could be activated in calcium free hypertonic media but not be fertilized, fertilization occurred in isotouic media rich in calcium ions. The rate of fertilization was dependent on calcium concentration, and eggs were not fertilized in solutions with a calcium ion concentration of less than 0·57 mmol kg⁻¹. Calcium ions could be replaced to some extent by magnesium ions, but the former were the more effective in fertilization. Since calcium ion concentration of ovarian fluid of A. alcicornis was 0·41 mmol kg⁻¹, it was inferred that low calcium concentration in the ovarian fluid was the cause of the failure of A. alcicornis eggs to fertilize internally.     

Reversible differential decondensation of unfixed Chinese hamster chromosomes induced by change in calcium ion concentration of the medium

Differential decondensation of isolated unfixed Chinese hamster metaphase chromosomes was obtained by decreasing the calcium ion concentration in the surrounding medium. A banded appearance of the swollen chromosomes could be observed either directly by phase contrast microscopy or after glutaraldehyde fixation and staining. There was a gradual transition from homogeneously dense to banded and finally to extensively decondensed chromosomes. The patterns induced at different stages were similar to those observed on fixed chromosomes after standard banding procedures (i.e., G-, C-, C_d–, Ag-NOR-staining). Chromosome decondensation could be reversed by the addition of calcium ions to the medium. Ca⁺⁺-dependent reversible differential chromosome decondensation was not observed if the chromosomes were previously treated with 0.35 M NaCl. Chromosome regions which had incorporated BrdU into their DNA were more resistant to a decrease in calcium ion concentration than BrdU non-substituted regions.