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1.
Is highly approximate knowledge of a protein's backbone structure sufficient to successfully identify its family, superfamily, and tertiary fold? To explore this question, backbone dihedral angles were extracted from the known three‐dimensional structure of 2,439 proteins and mapped into 36 labeled, 60° × 60° bins, called mesostates. Using this coarse‐grained mapping, protein conformation can be approximated by a linear sequence of mesostates. These linear strings can then be aligned and assessed by conventional sequence‐comparison methods. We report that the mesostate sequence is sufficient to recognize a protein's family, superfamily, and fold with good fidelity. Proteins 2005. © 2005 Wiley‐Liss, Inc. 相似文献
2.
Guengerich FP 《Journal of biochemical and molecular toxicology》2005,19(3):173-174
© 2005 Wiley Periodicals, Inc. J Biochem Mol Toxicol 19:173–174, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20072 相似文献
3.
Selgrade MK 《Journal of biochemical and molecular toxicology》2005,19(3):177-179
© 2005 Wiley Periodicals, Inc. J Biochem Mol Toxicol 19:177–179, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20075 相似文献
4.
© 2005 Wiley Periodicals, Inc. J Biochem Mol Toxicol 19:169–171, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20070 相似文献
5.
© 2005 Wiley Periodicals, Inc. J Biochem Mol Toxicol 19:180–181, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20076 相似文献
6.
Nebert DW 《Journal of biochemical and molecular toxicology》2005,19(3):184-186
© 2005 Wiley Periodicals, Inc. J Biochem Mol Toxicol 19:184–186, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20080 相似文献
7.
Garry VF 《Journal of biochemical and molecular toxicology》2005,19(3):175-175
© 2005 Wiley Periodicals, Inc. J Biochem Mol Toxicol 19:175, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20073 相似文献
8.
Furlong CE Cole TB Walter BJ Shih DM Tward A Lusis AJ Timchalk C Richter RJ Costa LG 《Journal of biochemical and molecular toxicology》2005,19(3):182-183
© 2005 Wiley Periodicals, Inc. J Biochem Mol Toxicol 19:182–183, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20079 相似文献
9.
Vermeulen R De Roos AJ Bakke B Blair A Hildesheim A Pinto L Gillette PP Lynch CF Allen RH Alavanja MC 《Journal of biochemical and molecular toxicology》2005,19(3):172-172
© 2005 Wiley Periodicals, Inc. J Biochem Mol Toxicol 19:172, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20071 相似文献
10.
© 2005 Wiley Periodicals, Inc. J Biochem Mol Toxicol 19:276–277, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20077 相似文献
11.
Kunkel TA 《Journal of biochemical and molecular toxicology》2005,19(3):190-191
© 2005 Wiley Periodicals, Inc. J Biochem Mol Toxicol 19:190–191, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20082 相似文献
12.
Eaton DL 《Journal of biochemical and molecular toxicology》2005,19(3):187-189
© 2005 Wiley Periodicals, Inc. J Biochem Mol Toxicol 19:187–189, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20081 相似文献
13.
Joseph Saragusty Thomas B. Hildebrandt Yehudit Natan Robert Hermes Saar Yavin Frank Goeritz Amir Arav 《Zoo biology》2005,24(3):233-245
This study was conducted in an effort to improve our understanding of the response of Asian elephant (Elephas maximus, Em) spermatozoa to chilling. Semen was collected from two elephant bulls by means of the manual rectal stimulation method. Five out of seven semen collections were deemed to be suitable for use based on motility (ranging from 20% to 60%) and membrane integrity. We evaluated the chilling sensitivity by incubating the sperm with a fluorescent dye (5‐carboxyfluorescein diacetate (cFDA)) at 16°C, 12°C, 4°C, and 22°C (control). Cells with an intact membrane retained the dye and were identified as viable. The membrane lipid phase transition (LPT) temperature curve was determined with a Fourier transform infrared (FTIR) spectrometer connected to an FTIR microscope. The LPT center, Tm, was determined by statistical analysis. The LPT and Tm were also assessed in fresh spermatozoa and spermatozoa incubated with egg yolk or egg‐phosphatidylcholine (EPC) liposomes at 16°C, 12°C, 4°C, and 26°C (control). The results show that the membrane integrity of spermatozoa incubated at 16°C, 12°C, and 4°C decreased by 39%, 62%, and 67%, respectively, compared to the control. The LPT temperatures were between room temperature (26°C) and 10°C, with Tm at 14–16°C. The Tm for sperm incubated with liposomes or egg‐yolk extender was below the measured range (2°C). Chilling sensitivity was found at a wide range of temperatures and transition temperatures, suggesting the presence of a wide variety of fatty acids (FAs) in the membrane with a high ratio of saturated‐to‐polyunsaturated FAs. Here we show that the protection afforded by the presence of egg yolk or liposomes in the extender is accomplished by shifting the Tm to below the 4°C point at which chilled semen is maintained for transport, or the point at which fast freezing begins to minimize cellular damage. Zoo Biol 0:1–13, 2005. © 2005 Wiley‐Liss, Inc. 相似文献
14.
Marinomonas sp. NJ522, isolated from Antarctic sea ice, produces a cold-active iron superoxide dismutase (SOD; EC 1.15.1.1). The purified
SOD was dimeric and had an approx. Mr of 48 kDa. Highest activity was detected from pH 8 to 10 and at 40 °C (assayed over
10 min). Activity at 0 °C was nearly 35% of the maximum activity.
Received 25 August 2005; Revisions requested 30 August 2005 and 26 September 2005; Revisions received 12 September 2005 and
25 October 2005; Accepted 1 November 2005 相似文献
15.
Martínez-Cruzado JC Toro-Labrador G Viera-Vera J Rivera-Vega MY Startek J Latorre-Esteves M Román-Colón A Rivera-Torres R Navarro-Millán IY Gómez-Sánchez E Caro-González HY Valencia-Rivera P 《American journal of physical anthropology》2005,128(1):131-155
The haplogroup identities of 800 mtDNAs randomly and systematically selected to be representative of the population of Puerto Rico were determined by restriction fragment length polymorphism (RFLP), revealing maternal ancestries in this highly mixed population of 61.3% Amerindian, 27.2% sub‐Saharan African, and 11.5% West Eurasian. West Eurasian frequencies were low in all 28 municipalities sampled, and displayed no geographic patterns. Thus, a statistically significant negative correlation was observed between the Amerindian and African frequencies of the municipalities. In addition, a statistically highly significant geographic pattern was observed for Amerindian and African mtDNAs. In a scenario in which Amerindian mtDNAs prevailed on either side of longitude 66°16′ West, Amerindian mtDNAs were more frequent west of longitude 66°16′ West than east of it, and the opposite was true for African mtDNAs. Haplogroup A had the highest frequency among Amerindian samples (52.4%), suggesting its predominance among the native Taínos. Principal component analysis showed that the sub‐Saharan African fraction had a strong affinity to West Africans. In addition, the magnitudes of the Senegambian and Gulf of Guinea components in Puerto Rico were between those of Cape Verde and São Tomé. Furthermore, the West Eurasian component did not conform to European haplogroup frequencies. HVR‐I sequences of haplogroup U samples revealed a strong North African influence among West Eurasian mtDNAs and a new sub‐Saharan African clade. Am J Phys Anthropol 128:131‐155, 2005. © 2005 Wiley‐Liss, Inc. 相似文献
16.
The original article to which this Erratum refers has been published in Journal of Neurobiology J Neurobiol (2005)64(1)75–90 . © 2005 Wiley Periodicals, Inc. 相似文献
17.
Alavanja MC Bonner MR Furlong CE Allen R Hodgson E 《Journal of biochemical and molecular toxicology》2005,19(3):192-193
© 2005 Wiley Periodicals, Inc. J Biochem Mol Toxicol 19:192–193, 2003; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20083 相似文献
18.
Biology Bulletin - This study was conducted in 1987–1988 and 2008 on Talan Island (59°20′ N, 146°05′ E), Sea of Okhotsk; in 2005 and 2011 on the Medvezhii Island... 相似文献
19.
The reproduction of female red pandas under three different husbandry conditions (monogamy, polygamy, and polyandry) was studied at the Chengdu Research Base for Giant Panda Breeding, and Chengdu Zoological Park, China. We monitored the fecal steroid profiles of seven females by radioimmunoassay in order to understand how the different mating groupings affect the animals' reproductive success. Of the seven females studied, all gave birth except the one that was kept in the polyandrous group. Although fecal estradiol metabolites exhibited several peaks during estrus, only one coincided with mating behavior. Fecal progesterone metabolites did not display a sustained rise in the absence of mating behavior, even though estradiol displayed a peak of secretion. This suggests that the red panda may be an induced ovulator. During gestation, fecal progesterone metabolites were maintained at a comparatively high level, and were much higher than those of nonpregnant females. The female in the polyandrous group showed an abnormal estradiol metabolite profile, with a level that was four to five times higher than those of the females in the polygynous groupings. It was observed that this female always escaped and hid in the trees to avoid courtships with males, and did not give birth. This phenomenon suggests that polyandrous groupings may be unsuitable for successful reproduction in captivity. Zoo Biol 24:169–176, 2005. © 2005 Wiley‐Liss, Inc. 相似文献
20.
Increase in d-tagatose Production Rate by Site-directed Mutagenesis of l-arabinose Isomerase from Geobacillus thermodenitrificans 总被引:2,自引:0,他引:2
Among single-site mutations of l-arabinose isomerase derived from Geobacillus thermodenitrificans, two mutants were produced having the lowest and highest activities of d-tagatose production. Site-directed mutagenesis at these sites showed that the aromatic ring at amino acid 164 and the size
of amino acid 475 were important for d-tagatose production. Among double-site mutations, one mutant converted d-galactose into d-tagatose with a yield of 58% whereas the wild type gave 46% d-tagatose conversion after 300 min at 65 °C.
Received 31 August 2005; Revisions requested 27 September 2005; Revisions received 8 November 2005; Accepted 8 November 2005 相似文献