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Sunday Adekunle Adebusoye Matthew O. Ilori Flynn W. Picardal Olukayode O. Amund 《World journal of microbiology & biotechnology》2008,24(1):61-68
Substantial metabolism of 2,3,4,5-tetrachlorobiphenyl (2,3,4,5-tetraCB) and 2,3′,4′,5-tetraCB by axenic cultures of Ralstonia sp. SA-5 and Pseudomonas sp. SA-6 was observed in the presence of biphenyl supplementation, although, the strains were unable to utilize tetrachlorobiphenyls
as growth substrate. The former was more amenable to aerobic degradation (∼70% degradation) than the latter (22–45% degradation).
Recovery of 2,5-chlorobenzoic acid and chloride from 2,3′,4′,5-tetraCB assay is an indication of initial dioxygenase attack
on the 3,4-dichlorophenyl ring. The PCB-degradative ability of both strains was also investigated by GC analysis of individual
congeners in Aroclor 1242 (100 ppm) following 12-day incubation with washed benzoate-grown cells. Results revealed two different
catabolic properties. Whereas strain SA-6 required biphenyl as inducer of the degradation activity, such induction was not
required by strain SA-5. Nearly all the detectable congeners in the mixture were extensively degraded (% reduction in ECD
area counts for individual congeners ranged from 50.0 to 100% and 14.2 to 100%, respectively, for SA-5 and SA-6). The two
strains exhibited no noticeable specificity for congeners with varying numbers of chlorine substitution and positions. The
degradative competence of these isolates most especially SA-5 makes them among the most versatile PCB-metabolizing organisms
yet reported. 相似文献