薄芝糖肽注射液药理学研究

目的研究薄芝糖肽注射液的药理作用.方法将试验小鼠随机分组,分别注射薄芝糖肽或生理盐水,观察并记录小鼠的反应.结果薄芝糖肽注射液能减少小鼠的自发活动性,加强利血平、氯丙嗪的中枢镇静作用,拮抗苯丙胺的中枢兴奋作用,延长戊巴比妥钠、巴比妥钠睡眠时间能增强小鼠记忆力,加强小鼠抗缺氧能力.结论薄芝糖肽注射液对中枢神经系统有明显的抑制作用,能增强记忆能力和缺氧耐力.     

薄芝糖肽注射液对细胞免疫功能影响的研究

目的研究薄芝糖肽注射液对细胞免疫功能的影响.方法对试验小鼠建立肝损伤模型,然后分为三组,一组作为正常对照组,另两组分别注射薄芝糖肽注射液或生理盐水.测定NK、IL-2、TNF的活性.结果采用薄芝糖肽注射液治疗能显著提高NK、IL-2、TNF的活性.结论薄芝糖肽注射液具有对肝细胞的保护作用,能治疗肝细胞损伤.其机制与免疫网络有很重要的关系,能增强机体免疫功能.     

薄芝糖肽注射液对肿瘤坏死因子的抑制作用

目的研究薄芝糖肽注射液对肿瘤坏死因子(tumor necrosis factor,TNF)的抑制作用.方法用葡萄球菌肠毒素B(staphylococcal enterotoxin B,SEB)体外诱生TNF-α、TNF-β,以1640培养基为对照,考察薄芝糖肽注射液对产生的TNF-α和TNF-β的细胞毒活性的影响.结果薄芝糖肽注射液对TNF-α和TNF-β的活性均有显著的抑制作用,且存在一定的量效关系.结论薄芝糖肽注射液可降低肿瘤坏死因子的活性.     

薄芝糖肽注射液治疗皮肤病301例应用价值分析

目的:观察薄芝糖肽注射液对18种皮肤顽症的临床应用研究.方法:应用于门诊的18种皮肤病患者,使用薄芝糖肽注射液,12岁以上患者每日2次,每次肌注2ml;12岁以下者,每日1次2ml,30d为1个疗程.治疗前后观察病人的皮损改善情况,并观察药品不良反应.结果:薄芝糖肽注射液对于常见皮肤病总有效率在56.3%-100%,对于患少见皮肤病病人治疗后6-40d好转或痊愈,少数病人出现不良反应,其中多为口干,不影响治疗.结论:薄芝糖肽注射液治疗多种皮肤病,改善病人自身机能状态,获得良好的临床效果,是一个副作用低、安全可靠的药物.     

薄芝糖肽注射液局部注射治疗局限性硬皮病的临床观察

目的:运用薄芝注射液局部注射治疗局限性硬皮病并观察疗效.方法:每次局部注射薄芝注射液2-4mL,每周1-2次,12周为1疗程.结果:46例中,近愈2例(4.3%),显效27例(58.7%),有效17例(37.0%),总有效率为100%.结论:薄芝糖肽注射液局部注射治疗局限性硬皮病具有疗效好、副作用小的特点.改善血液循环、调节免疫功能、抗炎抑制纤维增生、软化结缔组织、营养肌肉神经的作用可能是其取得较好疗效的主要机理.     

薄芝糖肽的单糖组成分析

目的对灵芝属薄树芝的活性成分薄芝糖肽的单糖进行研究.方法采用薄层色谱法和高效液相色谱-蒸发光散射检测器(HPLC-ELSD)联用技术.结果薄芝糖肽的单糖组成及摩尔比为葡萄糖∶D-甘露糖∶半乳糖∶木糖=1∶0.074∶0.067∶0.0178.结论建立了薄芝糖肽单糖组成的检测方法.     

酸奶中糖肽复合物的分离纯化

对酸奶中的肽类物质进行了提取、纯化和初步分析,认为该肽类成分是一种糖肽复合物。     

薄芝糖肽的抗肿瘤作用及其作用机制研究

目的研究薄芝糖肽的抗肿瘤作用,并探讨其作用机制.方法采用体内移植性肿瘤实验与体外细胞培养相结合的方法,对肿瘤细胞增值程度、细胞凋亡情况及肿瘤坏死因子(TNF)-α、干扰素(IFN)-γ及其mRNA的表达情况等进行研究.结果(1)薄芝糖肽在50、100、200μg/ml剂量下显著抑制移植性肉瘤S180的生长.(2)薄芝糖肽不能直接抑制人急性早幼粒白血病细胞(HL-60)的体外培养,不能诱导其凋亡.(3)薄芝糖肽与小鼠腹腔巨噬细胞和脾淋巴细胞共同培养显著抑制HL-60细胞生长并诱导其凋亡,TNF-α、IFN-γ及其mRNA的表达水平显著升高.结论薄芝糖肽可通过促进TNF-α和IFN-γ的mRNA的表达,增加TNF-α、IFN-γ的分泌而抑制肿瘤的生长.     

薄芝糖肽注射液对胃癌化疗患者减毒作用的临床观察

目的:探讨薄芝糖肽注射液减轻化疗药物对胃癌病人的毒性作用.方法:将120例正在进行化疗的胃癌患者随机分为两组:治疗组与对照组,分别给予薄芝糖肽注射液和鲨肝醇注射液.用药2个月后,观察患者周围血象的变化及免疫功能情况.结果:治疗组患者在血象变化方面与对照组比较有显著差异(P＜0.01),同时治疗组的患者的NK、IL-2、CD3、CD4、CD8、CD4/CD8的改变与对照组比较也具有统计学意义(P＜0.01或P＜0.05).结论:薄芝糖肽注射液可以明显减轻化疗药物对胃癌患者的骨髓抑制作用及具有提高患者免疫功能的作用.     

薄芝糖肽对D-GalN所致大鼠急性肝衰竭的影响

目的:探讨薄芝糖肽对组织损伤的保护作用.方法:Wister大鼠腹腔注射D-GalN(D-氨基半乳糖)诱发急性肝衰竭(ALF).48h后存活的大鼠随机分成4组,分别尾静脉注射不同剂量薄芝糖肽注射液或等量生理盐水,连续2w.观测动物存活率、血清谷丙转氨酶(ALT)以及肝组织学检查.结果:薄芝糖肽注射液3个剂量治疗组大鼠的存活率分别为42.1%、57.9%和63.2%,生理盐水对照组的存活率为21.1%,实验组与对照组的存活率相差显著(p＜0.05).治疗组大鼠ALT水平在给药第2d即明显下降,第7d基本恢复正常;对照组直到实验结束才恢复正常.4组动物病理切片显示,注射D-GalN后肝细胞大量坏死,呈现ALF状态.第15d高剂量治疗组基本恢复正常,但对照组仍见散在肝细胞坏死灶及,汇管区炎性细胞浸润.结论: 薄芝糖肽注射液可明显提高ALF大鼠存活率,改善肝功能.提示薄芝糖肽注射液可用于临床救治急性肝功能衰竭或重症肝炎.     

用亲和层析技术分离纯化天冬氨酸酶

<正> 亲和层析技术做为现代生化实验室常用的提纯方法,具有专一性强,活力损失小,分离步骤少等优点。天冬氨酸酶是重要的酶制剂,以往见报的分离纯化工艺步骤繁琐,活力损失大。本文报道的新工艺是用聚乙二醇(PEG)/磷酸钾双水相抽提;DEAE-sophadex A-50柱层析除PEG和部分杂蛋白;环氧氯丙烷活化载体,底物作配基的亲和层析技术获得了电泳纯的天冬氨酸酶。     

腺苷转运体亲和层析分离

本文合成了一种腺苷亲和层析凝胶,并采用亲和层析法从牛脑细胞膜上分离出了几种膜上结合的腺苷结合蛋白质。这些蛋白质在ＳＤＳ－ＰＡＧＥ电泳凝胶上为单一或主要的蛋白带,分子量分别为６４ｋｄ,４５ｋｄ,３５ｋｄ。腺苷转运体抑制剂潘生丁和ＮＢＭＰＲ对６４ｋｄ蛋白与＾３ｈ－腺苷的结合抑制作用远强于腺苷受体的激动剂ＮＥＣＡ和Ｒ－ＰＩＡ;这表明６４ｋｄ蛋白为牛脑细胞膜上结合的腺苷转运体。     

Purification of NADH-Nitrate Reductase by Affinity Chromatography

Assimilatory nitrate reductase (NADH: nitrate oxidoreductase, EC 1.6.6.1) from Chlorella vulgaris has been purified to electrophoretic homogeneity with an overall yield of 60% by a procedure that utilizes blue dextran-agarose as an affinity column. Nitrate reductase binds to blue dextran and is not eluted in the presence of high ionic strength buffer but is rapidly eluted in the presence of μmolar concentrations of NADH.     

Purification of Adenine Phosphoribosyltransferase by Affinity Chromatography

The purine salvage pathway enzyme adenine phosphoribosyltransferase (AMP: pyrophosphate phosphoribosyltransferase EC 2.42.7) has been purified to greater than 85% homogeneity from crude rat liver 100,000 × g supernatant in one step by affinity chromatography. The enzyme binds to an AMP-agarose column and is eluted off the column by 1 mM 5-phosphori-bosyl pyrophosphate with a 50 to 80% recovery. Enzyme kinetics indicate that the mechanism of the specific elution is due to competition of the product AMP and substrate 5-phosphoribosyl pyrophosphate for the same site on the enzyme.     

Purification of Cathepsin D by Ah-Sepharose Affinity Chromatography

A rapid and reliable method for coupling the protease in-hibitor pepstatin to AH-Sepharose 4B was developed. The matrix prepared was used to purify cathepsin D from rat liver. The enzyme was eluted in one fraction and proved to be pure by gel electrophoresis, two types of ion exchange chromatography, molecular sieve chromatography, and immunologically homogenous by Immunoelectrophoresis. This method is more rapid and gives a higher yield than previous techniques. The possibility to use this technique for the purification of other enzymes inhibitable by pepstatin should be considered.     

Purification of Canine Plasma Renin by Affinity Chromatography

An affinity column for the purification of canine plasma renin was prepared using goat anti-renin (dog kidney) γG globulins. The antiserum was prepared against a purified kidney renin preparation. The anti-renin globulins were coupled to cyanogen bromide-activated Sepharose. Using the anti-renin globulin-coupled Sepha-rose as an immuno-adsorbant, a method was devised allowing purification of plasma renin to a 1, 000-fold purity.     

Purification of L-Isoleucyl t-rna Synthetase by Affinity Chromatography

L-Isoleucyl t-RNA synthetase was purified to 97% purity in a single step by affinity chromatography on a column carrying L-isoleucinyl 5′-adenylate as the insolubilized ligand. Recovery was 50–60%.     

Effective Purification of Bioproducts by Fast Flow Affinity Chromatography

The performance of affinity adsorbents, such as HiPAC, Toyopearl, and Sepharose 4B coupled with protein A and anti-BSA, was compared. The HiPAC column, which is packed with rigid porous silica particles of 30 μm diameter, showed the highest value of D_pore/r² because of the small particle diameter and rigidity of the adsorbent particles. Further, it was operable at higher flow rates than the others. Thus, experimental data and calculation on the purification process show that fast and efficient purification is possible by use of the HiPAC column.     

应用单克隆抗体免疫亲和柱纯化干细胞因子

应用抗人干细胞生长因子(SCF)单克隆抗体,通过化学偶联方法制备成亲和层析柱,用于纯化大肠杆菌表达的可溶性重组人rh-SCF.结果表明,经亲和柱纯化的样品经SDS-PAGE电泳检测纯度达95%以上,计算蛋白回收率为23.4%,并且纯化后rh-SCF生物活性明显高于纯化前样品.     

亲和层析法纯化霍乱毒素及其B亚单位

利用GM1偶联活化的Sephadex G50或扰CT IgG偶联活化的Sepharose 4B,我们已成功地建立了两种亲和层析纯化CT和CT-B的方法,获得了纯化产品。受体亲和柱对CT-B和CT的分离效率分别为71.6％或48.3％,抗体亲和柱分别为50％或51.7％。纯化产物经SDSPAGE、PAGE分析表明均达到亲和层析电泳纯级。GM1-ELISA、CHO细胞测毒加间接免疫荧光检测、家兔肠段结扎试验和家兔免疫保护试验结果提示纯化产物具有良好的生物学活性。这种有效而简单的方法可推广应用于CT或CT相关毒素的纯化。