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人PNRC基因启动子的初步鉴定 总被引:1,自引:0,他引:1
PNRC (praline-rich nuclear receptor coactivator) 是最近发现的一种新的核受体辅活化子,它是以牛类固醇生成因子-1(SF1)作诱饵,利用酵母双杂系统从人乳腺cDNA表达文库中筛选得到的.为了研究人PNRC基因的表达调控机制,在对人PNRC基因的生物信息分析的基础上,采用5′RACE法确定了PNRC基因的转录起始位点,克隆了人PNRC基因的5′侧翼区,并对该区进行功能分析.分别构建了11种含不同长度启动子序列的荧光素酶报告基因表达质粒,将它们瞬时转染HepG2细胞,检测其荧光素酶活性.结果发现,PNRC的5′侧翼区的-323~+27、-323~+57-123~+57、-123~+27区域的启动子活性显著升高,其中-323~+27区域的启动子活性最高.研究表明,人PNRC基因启动子的最小区域位于PNRC的5′侧翼区的-123~+27区域. . 相似文献
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The functional importance of a cap site-proximal region of the human prointerleukin 1 beta gene is defined by viral protein trans-activation. 下载免费PDF全文
G W Hunninghake B G Monks L J Geist M M Monick M A Monroy M F Stinski A C Webb J M Dayer P E Auron M J Fenton 《Molecular and cellular biology》1992,12(8):3439-3448
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Kurose K Ikeda S Koyano S Tohkin M Hasegawa R Sawada J 《Molecular and cellular biochemistry》2006,281(1-2):35-43
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M. A. Kahn C. J. Huang A. Caruso V. Barresi R. Nazarian D. F. Condorelli J. de Vellis 《Journal of neurochemistry》1997,68(4):1413-1423
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The orphan nuclear receptor TR2 functions as a constitutive activator for the endogenous retinoic acid receptor beta2 (RAR(beta2)) gene expression in P19 embryonal carcinoma cells and for reporters driven by the RAR(beta2) promoter in COS-1 cells. The activation of RAR(beta2) by TR2 is mediated by the direct repeat-5 (DR5) element located in the RAR(beta2) promoter. Furthermore, cAMP exerts an enhancing effect on the activation of RAR(beta2) by TR2, which is mediated by the cAMP response element located in the 5'-flanking region of the DR5. The constitutive activation function-1 (AF-1) of TR2 is mapped to amino acid residues 10-30 in its N-terminal A segment. A direct molecular interaction occurs between CREMtau and TR2, detected by co-immunoprecipitation, which is mediated by the N-terminal AB segment of TR2. In gel mobility shift assays, TR2 competes with P19 nuclear factor binding to the RAR(beta2) promoter, and TR2 and CREMtau bind simultaneously to this DNA fragment. The role of TR2 in the early events of RA signaling process is discussed. 相似文献