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1.
S. A. Angioi D. Rau G. Attene L. Nanni E. Bellucci G. Logozzo V. Negri P. L. Spagnoletti Zeuli R. Papa 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(5):829-843
This study focuses on the expansion of Phaseolus vulgaris in Europe. The pathways of distribution of beans into and across Europe were very complex, with several introductions from
the New World that were combined with direct exchanges between European and other Mediterranean countries. We have analyzed
here six chloroplast microsatellite (cpSSR) loci and two unlinked nuclear loci (for phaseolin types and Pv-shatterproof1). We have assessed the genetic structure and level of diversity of a large collection of European landraces of P. vulgaris (307) in comparison to 94 genotypes from the Americas that are representative of the Andean and Mesoamerican gene pools.
First, we show that most of the European common bean landraces (67%) are of Andean origin, and that there are no strong differences
across European regions for the proportions of the Andean and Mesoamerican gene pools. Moreover, cytoplasmic diversity is
evenly distributed across European regions. Secondly, the cytoplasmic bottleneck that was due to the introduction of P. vulgaris into the Old World was very weak or nearly absent. This is in contrast to evidence from nuclear analyses that have suggested
a bottleneck of greater intensity. Finally, we estimate that a relatively high proportion of the European bean germplasm (about
44%) was derived from hybridization between the Andean and Mesoamerican gene pools. Moreover, although hybrids are present
everywhere in Europe, they show an uneven distribution, with high frequencies in central Europe, and low frequencies in Spain
and Italy. On the basis of these data, we suggest that the entire European continent and not only some of the countries therein
can be regarded as a secondary diversification center for P. vulgaris. Finally, we outline the relevance of these inter-gene pool hybrids for plant breeding. 相似文献
2.
3.
M. M. Khairallah B. B. Sears M. W. Adams 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1992,84(7-8):915-922
Summary Previous examination of intraspecific mitochondrial DNA (mtDNA) diversity in common bean, Phaseolus vulgaris, showed that five restriction fragment length polymorphisms (RFLPs) distinguish the mitochondrial genomes of the two major gene pools of cultivated beans, the Mesoamerican and the Andean. In the study presented here, mtDNA was used to compare the amount of diversity in cultivated beans to that in collections of wild beans to gain an understanding of how and when the mitochondrial genomes of the gene pools became distinct. The mtDNA of six wild bean accessions from Central and South America were digested with nine restriction endonucleases and analyzed by Southern hybridization. A total of twenty RFLPs were detected demonstrating a significantly higher amount of mtDNA variability in wild beans than in cultivated ones. All of the wild beans had the same mtDNA pattern for four out of the five inter-gene pool RFLPs, indicating that the polymorphism arose soon after domestication: two in the gene pool of the cultivated Mesoamerican beans and two in the gene pool of the cultivated Andean beans. The fifth RFLP must have occurred before domestication since the locus was also polymorphic in the wild beans. Wild beans from the south Andes were distinct and less variable than wild accessions of the north Andes and Mesoamerica. The distribution of mtDNA RFLPs among the wild beans supports the concept of two distinct domestication events for P. vulgaris. 相似文献
4.
B. Fofana J. P. Baudoin X. Vekemans D. G. Debouck P. du Jardin 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(2):202-212
Chloroplast DNA (cpDNA) diversity has been examined using PCR-RFLP and RFLP strategies for phylogenetic studies in the genus
Phaseolus. Twenty-two species, including 4 of the 5 cultivated species (P. lunatus L., the Lima bean; P. vulgaris L., the common bean; P. coccineus L., the runner bean and P. polyanthus Greenman, the year-bean), represented by 86 accessions were included in the study. Six PCR primers designed from cpDNA and
a total cpDNA probe were used for generating markers. Phylogenetic reconstruction using both Wagner parsimony and the neighbor-joining
method was applied to the restriction fragment data obtained from each of the molecular approaches. P. vulgaris L. was shown to separate with several species of largely Mesoamerican distribution, including P. coccineus L. and P. polyanthus Greenman, whereas P. lunatus L. forms a complex with 3 Andean species (P. pachyrrhizoides Harms, P. augusti Harms and P. bolivianus Piper) co-evolving with a set of companion species with a Mesoamerican distribution. Andean forms of the Lima bean are found
to be more closely related to the 3 Andean wild species than its Mesoamerican forms. An Andean origin of the Lima bean and
a double derivative process during the evolution of P. lunatus are suggested. The 3 Andean species are proposed to constitute the secondary gene pool of P. lunatus, while its companion allies of Mesoamerican distribution can be considered as members of its tertiary gene pool. On the basis
of these data, an overview on the evolution of the genus Phaseolus is also discussed.
Received: 1 May 1998 / Accepted: 13 July 1998 相似文献
5.
Structure of genetic diversity in the two major gene pools of common bean (Phaseolus vulgaris L., Fabaceae) 总被引:1,自引:0,他引:1
Myounghai Kwak Paul Gepts 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(5):979-992
Domesticated materials with well-known wild relatives provide an experimental system to reveal how human selection during
cultivation affects genetic composition and adaptation to novel environments. In this paper, our goal was to elucidate how
two geographically distinct domestication events modified the structure and level of genetic diversity in common bean. Specifically,
we analyzed the genome-wide genetic composition at 26, mostly unlinked microsatellite loci in 349 accessions of wild and domesticated
common bean from the Andean and Mesoamerican gene pools. Using a model-based approach, implemented in the software STRUCTURE,
we identified nine wild or domesticated populations in common bean, including four of Andean and four of Mesoamerican origins.
The ninth population was the putative wild ancestor of the species, which was classified as a Mesoamerican population. A neighbor-joining
analysis and a principal coordinate analysis confirmed genetic relationships among accessions and populations observed with
the STRUCTURE analysis. Geographic and genetic distances in wild populations were congruent with the exception of a few putative
hybrids identified in this study, suggesting a predominant effect of isolation by distance. Domesticated common bean populations
possessed lower genetic diversity, higher F
ST, and generally higher linkage disequilibrium (LD) than wild populations in both gene pools; their geographic distributions
were less correlated with genetic distance, probably reflecting seed-based gene flow after domestication. The LD was reduced
when analyzed in separate Andean and Mesoamerican germplasm samples. The Andean domesticated race Nueva Granada had the highest
F
ST value and widest geographic distribution compared to other domesticated races, suggesting a very recent origin or a selection
event, presumably associated with a determinate growth habit, which predominates in this race.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
6.
Nanni L Bitocchi E Bellucci E Rossi M Rau D Attene G Gepts P Papa R 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(8):1341-1357
Evolutionary studies in plant and animal breeding are aimed at understanding the structure and organization of genetic variations
of species. We have identified and characterized a genomic sequence in Phaseolus vulgaris of 1,200 bp (PvSHP1) that is homologous to SHATTERPROOF-1 (SHP1), a gene involved in control of fruit shattering in Arabidopsis thaliana. The PvSHP1 fragment was mapped to chromosome Pv06 in P. vulgaris and is linked to the flower and seed color gene V. Amplification of the PvSHP1 sequence from the most agronomically important legume species showed a high degree of interspecies diversity in the introns
within the Phaseoleae, while the coding region was conserved across distant taxa. Sequencing of the PvSHP1 sequence in a sample of 91 wild and domesticated genotypes that span the geographic distribution of this species in the centers
of origin showed that PvSHP1 is highly polymorphic and, therefore, particularly useful to further investigate the origin and domestication history of
P. vulgaris. Our data confirm the gene pool structure seen in P. vulgaris along with independent domestication processes in the Andes and Mesoamerica; they provide additional evidence for a single
domestication event in Mesoamerica. Moreover, our results support the Mesoamerican origin of this species. Finally, we have
developed three indel-spanning markers that will be very useful for bean germplasm characterization, and particularly to trace
the distribution of the domesticated Andean and Mesoamerican gene pools. 相似文献
7.
An investigation was made of the phylogenetic relationships among wild accessions of Lima bean (Phaseolus lunatus) and wild allies of Mesoamerican and Andean origins, using electrophoresis of seed storage proteins and isozymes. Mesoamerican wild species are phylogenetically more distant fromP. lunatus than Andean species, and apparently belong to the tertiary gene pool of Lima bean. The Andean wild species, which are investigated for the first time, reveal a high similarity to the Lima bean, and particularly with its Mesoamerican gene pool. These Andean species probably constitute a secondary gene pool of Lima bean, and are thus of considerable interest in the context of genetic improvement of the crop. Based on these observations, an Andean origin is suggested for the Andean wild species and forP. lunatus. These results point out the importance of collecting and conserving AndeanPhaseolus germplasm. 相似文献
8.
María De La Fuente Ana M. González Antonio M. De Ron Marta Santalla 《Molecular breeding : new strategies in plant improvement》2013,31(3):501-516
Most studies on the genetic diversity of common bean (Phaseolus vulgaris L.) have focussed on accessions from the Mesoamerican gene pool compared to the Andean gene pool. A deeper knowledge of the genetic structure of Argentinian germplasm would enable researchers to determine how the Andean domestication event affected patterns of genetic diversity in domesticated beans and to identify candidates for genes targeted by selection during the evolution of the cultivated common bean. A collection of 116 wild and domesticated accessions representing the diversity of the Andean bean in Argentina was genotyped by means of 114 simple sequence repeat (SSR) markers. Forty-seven Mesoamerican bean accessions and 16 Andean bean accessions representing the diversity of Andean landraces and wild accessions were also included. Using the Bayesian algorithm implemented in the software STRUCTURE we identified five major groups that correspond to Mesoamerican and Argentinian wild accessions and landraces and a group that corresponds to accessions from different Andean and Mesoamerican countries. The neighbour-joining algorithm and principal coordinate clustering analysis confirmed the genetic relationships among accessions observed with the STRUCTURE analysis. Argentinian accessions showed a substantial genetic variation with a considerable number of unique haplotypes and private alleles, suggesting that they may have played an important role in the evolution of the species. The results of statistical analyses aimed at identifying genomic regions with consistent patterns of variation were significant for 35 loci (~20 % of the SSRs used in the Argentinian accessions). One of these loci mapped in or near the genomic region of the glutamate decarboxylase gene. Our data characterize the population structure of the Argentinian germplasm. This information on its diversity will be very valuable for use in introgressing Argentinian genes into commercial varieties because the majority of present-day common bean varieties are of Andean origin. 相似文献
9.
A. Maquet I. Zoro Bi M. Delvaux B. Wathelet J.-P. Baudoin 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(5-6):980-991
Genetic diversity and structure within a Lima bean (Phaseolus lunatus L.) base collection have been evaluated using allozyme markers. The results obtained from the analysis of wild and cultivated
accessions confirm the existence of Andean and Mesoamerican gene pools characterised by specific alleles. Wild and cultivated
accessions of the same gene pool are grouped. The Andean natural populations have a very limited geographic distribution between
Ecuador and northern Peru. The Mesoamerican wild form extends from Mexico up to Argentina through the eastern side of the
Andes. Andean and Mesoamerican cultivated accessions of pantropical distribution contribute substantially to the genetic diversity
of the Lima bean base collection. Population genetic parameters, estimated from allozymes, confirmed the predominant selfing
mating system of the Lima bean. The selfing mating system, the occurrence of small populations, and low gene flow lead to
an interpopulation gene diversity (DST=0.235) higher than the intrapopulation gene diversity (HS=0.032). On the basis of the results, guidelines are given to preserve and exploit the genetic diversity of this threatened
species. The results also confirm the independent domestication of the Lima bean in at least two centres, one of which is
located at medium elevation in the western valleys of Ecuador and northern Peru.
Received: 3 June 1997 / Accepted: 17 June 1997 相似文献
10.
Tania Gioia Giuseppina Logozzo Giovanna Attene Elisa Bellucci Stefano Benedettelli Valeria Negri Roberto Papa Pierluigi Spagnoletti Zeuli 《PloS one》2013,8(10)
Common bean diversity within and between Mesoamerican and Andean gene pools was compared in 89 landraces from America and 256 landraces from Europe, to elucidate the effects of bottleneck of introduction and selection for adaptation during the expansion of common bean (Phaseolus vulgaris L.) in Europe. Thirteen highly polymorphic nuclear microsatellite markers (nuSSRs) were used to complement chloroplast microsatellite (cpSSRs) and nuclear markers (phaseolin and Pv-shatterproof1) data from previous studies. To verify the extent of the introduction bottleneck, inter-gene pool hybrids were distinguished from “pure” accessions. Hybrids were identified on the basis of recombination of gene pool specific cpSSR, phaseolin and Pv-shatterproof1 markers with a Bayesian assignments based on nuSSRs, and with STRUCTURE admixture analysis. More hybrids were detected than previously, and their frequency was almost four times larger in Europe (40.2%) than in America (12.3%). The genetic bottleneck following the introduction into Europe was not evidenced in the analysis including all the accessions, but it was significant when estimated only with “pure” accessions, and five times larger for Mesoamerican than for Andean germplasm. The extensive inter-gene pool hybridization generated a large amount of genotypic diversity that mitigated the effects of the bottleneck that occurred when common bean was introduced in Europe. The implication for evolution and the advantages for common bean breeding are discussed. 相似文献
11.
We have been examining the importance of the root system on shoot growth and development using a developmentally disabled
hybrid of the common bean Phaseolus vulgaris L. Parental cultivars (P. Vulgaris cv. Redkloud of Mesoamerican origin, and P. vulgaris cv. Batt of Andean origin) grow normally, but crosses produce F1 hybrids exhibiting hybrid weakness associated with reduced
root and shoot growth. In this study, applications of benzylaminopurine (BAP) to roots of F1 hybrids increased the number
of root tips and leaves. Reciprocal grafting was used to study the effects of the root system on shoots. Grafting of roots
of the Mesoamerican cultivar onto shoots of F1 hybrids increased the cytokinin concentrations in leaves of F1 hybrids and
removed the characteristics associated with hybrid weakness. To determine whether factors in the xylem sap enhanced leaf growth,
leaf discs were incubated on sap collected from Mesoamerican and Andean cultivars. Sap from Mesoamerican plants enhanced the
growth of leaf discs excised from F1 hybrids more than sap collected from Andean cultivars. Estimates of the transport of
zeatin riboside (ZR)–type cytokinins from roots of F1 hybrids indicated that transport out of hybrid roots was reduced compared
with those transported out of Mesoamerican or Andean roots. Results suggest that ZR-type cytokinins are involved in hormonal
integration between roots and shoots of P. vulgaris and that one of the barriers to hybridization between Andean and Mesoamerican landraces is related to hormone transport.
Received October 15, 1998; accepted May 12, 1999 相似文献
12.
Genetic variation inPhaseolus lunatus (Lima bean) was investigated at isozyme and DNA levels. Sixty cultivated accessions, including representatives of the Mesoamerican and Andean gene pools and intermediate types, were analyzed for variability at 17 isozyme loci. Some accessions were also examined for restriction fragment length polymorphism (RFLP) at the rDNA level. These data were used to construct two dendrograms showing clear separation in two distinct groups corresponding to each of the gene pools and an intermediate one probably representing a transitional group. 相似文献
13.
Thiago Lívio P. O. Souza Everaldo G. de Barros Claudia M. Bellato Eun-Young Hwang Perry B. Cregan Marcial A. Pastor-Corrales 《Molecular breeding : new strategies in plant improvement》2012,30(1):419-428
Single nucleotide polymorphisms (SNPs) were discovered in common bean (Phaseolus vulgaris L.) via resequencing of sequence-tagged sites (STSs) developed by PCR primers previously designed to soybean shotgun and bacterial artificial chromosome (BAC) end sequences, and by primers designed to common bean genes and microsatellite flanking regions. DNA fragments harboring SNPs were identified in single amplicons from six contrasting P. vulgaris genotypes of the Andean (Jalo EEP 558, G 19833, and AND 277) and Mesoamerican (BAT 93, DOR 364, and Rudá) gene pools. These genotypes are the parents of three common bean recombinant inbred line mapping populations. From an initial set of 1,880 PCR primer pairs tested, 265 robust STSs were obtained, which could be sequenced in each one of the six common bean genotypes. In the resulting 131,120?bp of aligned sequence, a total of 677 SNPs were identified, including 555 single-base changes (295 transitions and 260 transversions) and 122 small nucleotide insertions/deletions (indels). The frequency of SNPs was 5.16 SNPs/kb and the mean nucleotide diversity, expressed as Halushka??s theta, was 0.00226. This work represents one of the first efforts aimed at detecting SNPs in P. vulgaris. The SNPs identified should be an important resource for common bean geneticists and breeders for quantitative trait locus discovery, marker-assisted selection, and map-based cloning. These SNPS will be also useful for diversity analysis and microsynteny studies among legume species. 相似文献
14.
A major quantitative trait loci (QTL) conditioning common bacterial blight (CBB) resistance in common bean (Phaseolus vulgaris L.) lines HR45 and HR67 was derived from XAN159, a resistant line obtained from an interspecific cross between common bean
lines and the tepary bean (P. acutifolius L.) line PI319443. This source of CBB resistance is widely used in bean breeding. Several other CBB resistance QTL have been
identified but none of them have been physically mapped. Four molecular markers tightly linked to this QTL have been identified
suitable for marker assisted selection and physical mapping of the resistance gene. A bacterial artificial chromosome (BAC)
library was constructed from high molecular weight DNA of HR45 and is composed of 33,024 clones. The size of individual BAC
clone inserts ranges from 30 kb to 280 kb with an average size of 107 kb. The library is estimated to represent approximately
sixfold genome coverage. The BAC library was screened as BAC pools using four PCR-based molecular markers. Two to seven BAC
clones were identified by each marker. Two clones were found to have both markers PV-tttc001 and STS183. One preliminary contig
was assembled based on DNA finger printing of those positive BAC clones. The minimum tiling path of the contig contains 6
BAC clones spanning an estimated size of 750 kb covering the QTL region. 相似文献
15.
I. N. Wagara A. W. Mwang'ombe J. W. Kimenju R. A. Buruchara R. Jamnadass P. A. O. Majiwa 《Journal of Phytopathology》2004,152(4):235-242
Genetic diversity of 50 Phaeoisariopsis griseola isolates collected from different agroecological zones in Kenya was studied using group‐specific primers and amplified fragment length polymorphism (AFLP) markers. Group‐specific primers differentiated the isolates into Andean and Mesoamerican groups, corresponding to the two common‐bean gene pools. Significant polymorphisms were observed with all the AFLP primer combinations used, reflecting a wide genetic diversity in the P. griseola population. A total of 207 fingerprints was generated, of which 178 were polymorphic. Cluster analysis of the polymorphic bands also separated the isolates into the two groups defined by group‐specific primers. All the isolates examined were grouped into three virulence populations; Andean, Afro‐Andean and Mesoamerican, and their genetic diversity measured. On average, greater diversity (91%) was detected within populations than between populations (9%). The genetic distance between Andean and Mesoamerican populations was higher (D = 0.0269) than between Andean and Afro‐Andean (D = 0.0095). The wide genetic diversity reported here has significant implications in breeding for resistance to angular leaf spot and should be taken into consideration when screening and deploying resistant bean genotypes. 相似文献
16.
P. S. Vidigal Filho M. C. Gonçalves-Vidigal J. D. Kelly W. W. Kirk 《Journal of Phytopathology》2007,155(2):108-113
Pathogenicity of physiologically distinct races of Colletotrichum lindemuthianum originating from Andean (races 7, 19 and 55) and Mesoamerican (races 9, 31, 65, 69, 73, 81, 89, 95 and 453) locations of the new world were evaluated on 26 landrace genotypes of common bean (Phaseolus vulgaris L.) from Paraná State, Brazil. Races 7 (Andean), 65, 73 and 89 (Mesoamerican) were the most pathogenic, while race 31 (Mesoamerican) was the least pathogenic. Most of the landrace genotypes evaluated (88%) were resistant to race 31, except Carioca 3, Preto 1 and Preto 2. In addition, about 50% of the landrace genotypes had resistance to races 9, 19, 55 and 453; and about 30% to races 7, 65, 69, 73, 81, 89 and 95. The resistance index, which measured the pathogenicity response averaged across all the physiologically distinct Andean and Mesoamerican races of C. lindemuthianum, of the landrace genotypes ranged from 8% to 83%. The most resistant cultivars were Carioca Pintado 1, Carioca Pintado 2, Jalo Vermelho and Jalo de Listras Pretas. In contrast, the most susceptible cultivars were Jalo Pardo, Jalo Pintado 1 and Bolinha that showed resistance only to the least pathogenic race 31. These results indicated that many of the common bean landrace cultivars evaluated have genes that could be useful in breeding programmes to enhance resistance to Andean and Mesoamerican races of C. lindemuthianum. 相似文献
17.
Using molecular markers to assess the effect of introgression on quantitative attributes of common bean in the Andean gene pool 总被引:7,自引:0,他引:7
Islam FM Beebe S Muñoz M Tohme J Redden RJ Basford KE 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,108(2):243-252
Progress in bean breeding programs requires the exploitation of genetic variation that is present among races or through introgression across gene pools of Phaseolus vulgaris L. Of the two major common bean gene pools, the Andean gene pool seems to have a narrow genetic base, with about 10% of the accessions in the CIAT core collection presenting evidence of introgression. The objective of this study was to quantify the degree of spontaneous introgression in a sample of common bean landraces from the Andean gene pool. The effects of introgression on morphological, economic and nutritional attributes were also investigated. Homogeneity analysis was performed on molecular marker data from 426 Andean-type accessions from the primary centres of origin of the CIAT common bean core collection and two check varieties. Quantitative attribute diversity for 15 traits was studied based on the groups found from the cluster analysis of marker prevalence indices computed for each accession. The two-group summary consisted of one group of 58 accessions (14%) with low prevalence indices and another group of 370 accessions (86%) with high prevalence indices. The smaller group occupied the outlying area of points displayed from homogeneity analysis, yet their geographic origin was widely distributed over the Andean region. This group was regarded as introgressed, since its accessions displayed traits that are associated with the Middle American gene pool: high resistance to Andean disease isolates but low resistance to Middle American disease isolates, low seed weight and high scores for all nutrient elements. Genotypes generated by spontaneous introgression can be helpful for breeders to overcome the difficulties in transferring traits between gene pools.Communicated by H.C. Becker 相似文献
18.
Marília Lobo Burle Jaime Roberto Fonseca James A. Kami Paul Gepts 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(5):801-813
Brazil is the largest producer and consumer of common bean (Phaseolus vulgaris L.), which is the most important source of human dietary protein in that country. This study assessed the genetic diversity and the structure of a sample of 279 geo-referenced common bean landraces from Brazil, using molecular markers. Sixty-seven microsatellite markers spread over the 11 linkage groups of the common bean genome, as well as Phaseolin, PvTFL1y, APA and four SCAR markers were used. As expected, the sample showed lower genetic diversity compared to the diversity in the primary center of diversification. Andean and Mesoamerican gene pools were both present but the latter gene pool was four times more frequent than the former. The two gene pools could be clearly distinguished; limited admixture was observed between these groups. The Mesoamerican group consisted of two sub-populations, with a high level of admixture between them leading to a large proportion of stabilized hybrids not observed in the centers of domestication. Thus, Brazil can be considered a secondary center of diversification of common bean. A high degree of genome-wide multilocus associations even among unlinked loci was observed, confirming the high level of structure in the sample and suggesting that association mapping should be conducted in separate Andean and Mesoamerican Brazilian samples. 相似文献
19.
Characterization of all chromosomes of the Andean G19833 bean genotype was carried out by fluorescent in situ hybridization. Eleven single-copy genomic sequences, one for each chromosome, two BACs containing subtelomeric and pericentromeric repeats and the 5S and 45S ribosomal DNA (rDNA) were used as probes. Comparison to the Mesoamerican accession BAT93 showed little divergence, except for additional 45S rDNA sites in four chromosome pairs. Altogether, the results indicated a relative karyotypic stability during the evolution of the Andean and Mesoamerican gene pools of P. vulgaris. 相似文献
20.
Anna Farina Valentina Rocchi Michela Janni Stefano Benedettelli Giulia De Lorenzo Renato D’Ovidio 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(7):1371-1379
Polygalacturonase-inhibiting proteins (PGIPs) are extracellular plant protein inhibitors of endo-polygalacturonases (PGs)
that belong to the leucine-rich repeat (LRR) protein family. In bean, PGIP is encoded by a small gene family of four members
among which Pvpgip2 encodes the most wide-spectrum and efficient inhibitor of fungal PGs. In order to evaluate the sequence polymorphism of Pvpgip2 and its functional significance, we have analyzed a number of wild and cultivated bean (P. vulgaris) accessions of Andean and Mesoamerican origin, and some genotypes from the related species P. coccineus, P. acutifolius, and P. lunatus. Our analyses indicate that the protein encoded by Pvpgip2 is highly conserved in the bean germplasm. The few detected polymorphic sites correspond to synonymous substitutions and
only two wild genotypes contain a Pvpgip2 with a single non-synonymous replacement. Sequence comparison showed a slightly larger variation in the related bean species
P. coccineus, P. acutifolius, and P. lunatus and confirmed the known phylogenetic relationships with P. vulgaris. The majority of the replacements were within the xxLxLxx region of the leucine rich repeat (LRR) domain and none of them
affected residues contributing to structural features. The variant PGIPs were expressed in Nicotiana benthamiana using PVX as vector and their inhibitory activity compared to that of PvPPGIP2. All the variants were able to fully inhibit
the four fungal PGs tested with minor differences. Taken together these results support the hypothesis that the overall sequence
conservation of PGIP2 and minor variation at specific sites is necessary for high-affinity recognition of different fungal
PGs.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献