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1.
We describe here the isolation and characterization of OsiSAP8, a member of stress Associated protein (SAP) gene family from rice characterized by the presence of A20 and AN1 type Zinc
finger domains. OsiSAP8 is a multiple stress inducible gene, induced by various stresses, namely heat, cold, salt, desiccation, submergence, wounding,
heavy metals as well as stress hormone Abscisic acid. OsiSAP8 protein fused to GFP was localized towards the periphery of
the cells in the epidermal cells of infiltrated Nicotiana benthamiana leaves. Yeast two hybrid analysis revealed that A20 and AN1 type zinc-finger domains of OsiSAP8 interact with each other.
Overexpression of the gene in both transgenic tobacco and rice conferred tolerance to salt, drought and cold stress at seed
germination/seedling stage as reflected by percentage of germination and gain in fresh weight after stress recovery. Transgenic
rice plants were tolerant to salt and drought during anthesis stage without any yield penalty as compared to unstressed transgenic
plants.
OsiSAP8 is deposited in the Genbank with the Accession number AY345599. 相似文献
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Guiping Diao Yucheng Wang Chao Wang Chuanping Yang 《Plant Molecular Biology Reporter》2011,29(1):77-87
Plant glutathione S-transferases (GSTs) are involved in protecting plants against both diverse biotic and abiotic stresses. In the present study,
a novel GST gene (LbGST1) was cloned from Limonium bicolor (Bunge) Kuntze (Plumbaginaceae). To characterize its function in salt tolerance, tobacco lines transformed with LbGST1 were generated. Compared with wild-type (WT) tobacco, transgenic plants overexpressing LbGST1 exhibited both GST and glutathione peroxidase activities. Moreover, superoxide dismutase, peroxidase (POD), and catalase
activities in transgenic plants were significantly higher than those in WT plants, particularly when grown under conditions
of salt stress. Similarly, levels of proline in transgenic plants were also higher than those in WT plants grown under NaCl
stress conditions. Whereas, Malondialdehyde contents in transgenic plants were lower than those in WT plants under NaCl conditions.
Furthermore, Na+ content in transgenic plants was lower than that in WT plants under these stress conditions. Subcellular localization analysis
revealed that the LbGST1 protein was localized in the nucleus. These results suggested that overexpression of LbGST1 gene can affect many physiological processes associated with plant salt tolerance. Therefore, we hypothesize that LbGST1 gene can mediate many physiological pathways that enhance stress resistance in plants. 相似文献
4.
Water movement across cellular membranes is regulated largely by a family of water channel proteins called aquaporins (AQPs).
Since several abiotic stresses such as, drought, salinity and freezing, manifest themselves via altering water status of plant
cells and are linked by the fact that they all result in cellular dehydration, we overexpressed an AQP (tonoplast intrinsic
protein) from Panax ginseng, PgTIP1, in transgenic Arabidopsis
thaliana plants to test its role in plant’s response to drought, salinity and cold acclimation (induced freezing tolerance). Under
favorable conditions, PgTIP1 overexpression significantly increased plant growth as determined by the biomass production, and leaf and root morphology.
PgTIP1 overexpression had beneficial effect on salt-stress tolerance as indicated by superior growth status and seed germination
of transgenic plants under salt stress; shoots of salt-stressed transgenic plants also accumulated greater amounts of Na+ compared to wild-type plants. Whereas PgTIP1 overexpression diminished the water-deficit tolerance of plants grown in shallow (10 cm deep) pots, the transgenic plants
were significantly more tolerant to water stress when grown in 45 cm deep pots. The rationale for this contrasting response,
apparently, comes from the differences in the root morphology and leaf water channel activity (speed of dehydration/rehydration)
between the transgenic and wild-type plants. Plants overexpressed with PgTIP1 exhibited lower (relative to wild-type control) cold acclimation ability; however, this response was independent of cold-regulated
gene expression. Our results demonstrate a significant function of PgTIP1 in growth and development of plant cells, and suggest that the water movement across tonoplast (via AQP) represents a rate-limiting
factor for plant vigor under favorable growth conditions and also significantly affect responses of plant to drought, salt
and cold stresses. 相似文献
5.
Plant growth and productivity are adversely affected by various abiotic and biotic stress factors. In our previous study,
we used Prosopis juliflora, an abiotic stress tolerant tree species of Fabaceae, as a model plant system for isolating genes functioning in abiotic stress tolerance. Here we report the isolation and characterization
of a Rab family GTPase from P. juliflora (Pj Rab7) and the ability of this gene to confer salt stress tolerance in transgenic tobacco. Northern analysis for Pj Rab7 in P. juliflora leaf tissue revealed up-regulation of this gene under salt stress under the concentrations and time points analyzed. Pj Rab7 transgenic tobacco lines survived better under conditions of 150 mM NaCl stress compared to control un-transformed plants.
Pj Rab7 transgenic plants were found to accumulate more sodium than control plants during salt stress. The results of our studies
could be used as a starting point for generation of crop plants tolerant to abiotic stress. 相似文献
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Chenxi Xu Lei Zheng Caiqiu Gao Chao Wang Guifeng Liu Jing Jiang Yucheng Wang 《Plant Molecular Biology Reporter》2011,29(2):424-430
H+-ATPase subunit c (VHA-c) is involved in the adaptation to environmental stresses, including salt, drought, and heavy metals.
However, it remains unclear whether VHA-c can induce a physiological response related to stress tolerance. To investigate
this possibility, we generated transgenic tobacco lines overexpressing a V-ATPase subunit c (LbVHA-c1) gene from Limonium bicolor (Bunge) Kuntze. Compared with wild-type (WT) tobacco, superoxide dismutase (SOD) and peroxidase (POD) activities in the transgenic
plants were significantly enhanced under salt stress conditions. The level of malondialdehyde (MDA) in the transgenic plants
was significantly lower than that in WT plants grown under salt stress conditions. Moreover, the transgenic plants displayed
obviously better growth than the WT plants under salt stress. These results suggest that LbVHA-c1 may confer stress tolerance through enhancing POD and SOD activities, and by protecting membranes from damage by decreasing
lipid peroxidation under salt stress. 相似文献
8.
Supaporn Hasthanasombut Valentine Ntui Kanyaratt Supaibulwatana Masahiro Mii Ikuo Nakamura 《Plant biotechnology reports》2010,4(1):75-83
Glycine betaine has been reported as an osmoprotectant compound conferring tolerance to salinity and osmotic stresses in plants.
We previously found that the expression of betaine aldehyde dehydrogenase 1 gene (OsBADH1), encoding a key enzyme for glycine betaine biosynthesis pathway, showed close correlation with salt tolerance of rice. In
this study, the expression of the OsBADH1 gene in transgenic tobacco was investigated in response to salt stress using a transgenic approach. Transgenic tobacco plants
expressing the OsBADH1 gene were generated under the control of a promoter from the maize ubiquitin gene. Three homozygous lines of T2 progenies with single transgene insert were chosen for gene expression analysis. RT-PCR and western blot analysis results
indicated that the OsBADH1 gene was effectively expressed in transgenic tobacco leading to the accumulation of glycine betaine. Transgenic lines demonstrated
normal seed germination and morphology, and normal growth rates of seedlings under salt stress conditions. These results suggest
that the OsBADH1 gene could be an excellent candidate for producing plants with osmotic stress tolerance. 相似文献
9.
Wheat vacuolar H+-ATPases (V-ATPase) subunit B, named TaVB, was isolated from the salt-tolerant wheat RH8706-49 and used to transform Arabidopsis plants. TaVB-expressed Arabidopsis has a higher germination rate, root length, V–H+-ATPase activity, and overall salt tolerance than the wild type, indicating that expression of the gene can affect salt tolerance
of the transgenic plants. Under salt stress, intracellular Na+ levels in transgenic plants are significantly lower than the control. These results suggest that expression of the wheat
TaVB gene may enhance plant tolerance to salt stress. 相似文献
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S. M. Touhidul Islam R. S. Tammi Sneh L. Singla-Pareek Zeba Islam Seraj 《Acta Physiologiae Plantarum》2010,32(4):657-663
Rice yield is severely affected by high-salt concentration in the vicinity of the plant. In an effort to engineer rice for
improved salt tolerance Agrobacterium-mediated transformation of rice cv. Binnatoa was accomplished with the Pennisetum glaucum vacuolar Na+/H+ antiporter gene (PgNHX1) under the constitutive CaMV35S promoter. For the molecular analysis of putative transgenic plants, PCR and RT-PCR were performed.
Transgenic rice plants expressing PgNHX1 showed better physiological status and completed their life cycle by setting flowers and seeds in salt stress, while wild-type
plants exhibited rapid chlorosis and growth inhibition. Moreover, transgenic rice plants produced higher grain yields than
wild-type plants under salt stress. Assessment of the salinity tolerance of the transgenic plants at seedling and reproductive
stages demonstrated the potential of PgNHX1 for imparting enhanced salt tolerance capabilities and improved yield. 相似文献
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C. W. Lu Y. Shao L. Li A. J. Chen W. Q. Xu K. J. Wu Y. B. Luo B. Z. Zhu 《Russian Journal of Plant Physiology》2011,58(1):118-125
Ethylene-responsive factors (ERFs) play an important role in plant responses to stresses. For this study, we obtained sense-
and antisense-SlERF1 transgenic tomato plants to analyze the function of the SlERF1 gene in tomato plants. Overexpression of SlERF1 in tomato plants enhanced salt tolerance during tomato seedling root development. In addition, tomato seedlings overexpressing
SlERF1 showed the higher relative water content and lower MDA content and electrolyte leakage; they accumulated more free proline
and soluble sugars, as compared with wild-type and antisense-SlERF1 transgenic tomato plants under salt stress. Moreover, SlERF1 activated the expression of stress-related genes, including
LEA, P5CS, DREB3-1, and ltpg2 in tomato plants under salt stress. Thus, SlERF1 played a positive role in the salt tolerance of tomato plants. 相似文献
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Qing-Lin Liu Ke-Dong Xu Nan Ma Li Zeng Liang-Jun Zhao 《Molecular biology reports》2010,37(2):1137-1142
16.
CrPrx and CrPrx1 are class III peroxidases previously cloned and characterized from Catharanthus roseus. CrPrx is known to be apoplastic in nature, while CrPrx1 is targeted to vacuoles. In order to study their role in planta, these two peroxidases were expressed in Nicotiana tabacum. The transformed plants exhibited increased peroxidase activity. Increased oxidative stress tolerance was also observed in
transgenics when treated with H2O2 under strong light conditions. However, differential tolerance to salt and dehydration stress was observed during germination
of T1 transgenic seeds. Under these stresses, the seed germination of CrPrx-transformed plants and wild-type plants was clearly suppressed, whereas CrPrx1 transgenic lines showed improved germination. CrPrx-transformed lines exhibited better cold tolerance than CrPrx1-transformed lines. These results indicate that vacuolar peroxidase plays an important role in salt and dehydration stress
over cell wall-targeted peroxidase, while cell wall-targeted peroxidase renders cold stress tolerance. 相似文献
17.
On the basis of the results of gene chip analysis of the salt-tolerant wheat mutant RH8706-49 under conditions of salt stress,
we identified and cloned an unknown salt-induced gene TaST (Triticum aestivum salt-tolerant). Real-time quantitative PCR analysis showed that the expression of the gene was induced by salt stress. Transgenic
Arabidopsis plants overexpressing the TaST gene showed higher salt tolerance than the wild-type controls. Subcellular localization studies revealed that the protein
encoded by this gene was in the nucleus. In comparison with wild-type controls, transgenic Arabidopsis plants accumulated more Ca2+, soluble sugar, and proline and less Na+ under salt stress. Real-time quantitative PCR analysis showed that Arabidopsis plants overexpressing TaST also showed increased expression of many stress-related genes. All these findings indicated that TaST can enhance the salt tolerance of transgenic Arabidopsis plants. 相似文献
18.
Teppei Moriwaki Yujirou Yamamoto Takehiko Aida Tatsuya Funahashi Toshiyuki Shishido Masataka Asada Shamusul Haque Prodhan Atsushi Komamine Tsuyoshi Motohashi 《Plant biotechnology reports》2008,2(1):41-46
Salinity stress is a major limiting factor in cereal productivity. Many studies report improvements in salt tolerance using
model plants, such as Arabidopsis thaliana or standard varieties of rice, e.g., the japonica rice cultivar Nipponbare. However, there are few reports on the enhancement
of salt tolerance in local rice cultivars. In this work, we used the indica rice (Oryza sativa) cultivar BR5, which is a local cultivar in Bangladesh. To improve salt tolerance in BR5, we introduced the Escherichia coli catalase gene, katE. We integrated the katE gene into BR5 plants using an Agrobacterium tumefaciens-mediated method. The introduced katE gene was actively expressed in the transgenic BR5 rice plants, and catalase activity in T1 and T2 transgenic rice was approximately 150% higher than in nontransgenic plants. Under NaCl stress conditions, the transgenic
rice plants exhibited high tolerance compared with nontransgenic rice plants. T2 transgenic plants survived in a 200 mM NaCl solution for 2 weeks, whereas nontransgenic plants were scorched after 4 days
soaking in the same NaCl solution. Our results indicate that the katE gene can confer salt tolerance to BR5 rice plants. Enhancement of salt tolerance in a local rice cultivar, such as BR5, will
provide a powerful and useful tool for overcoming food shortage problems. 相似文献
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Supaporn Hasthanasombut Kanyaratt Supaibulwatana Masahiro Mii Ikuo Nakamura 《Plant Cell, Tissue and Organ Culture》2011,104(1):79-89
Betaine aldehyde dehydrogenase (BADH) is a major oxidative enzyme that converts betaine aldehyde to glycine betaine (GB),
an osmoprotectant compound in plants. Japonica rice (salt-sensitive) was genetically engineered to enhance salt tolerance
by introducing the OsBADH1 gene from Indica rice (salt-tolerant), which is a GB accumulator. We produced transgenic rice plants overexpressing the modified
OsBADH1 gene under the control of the maize ubiquitin promoter. The transgenic rice showed increased OsBADH1 gene expression and OsBADH1 enzyme production, resulting in the accumulation of GB. It also exhibited enhanced salt tolerance
in immature and mature transgenic rice seedlings. The adverse effect of salt stress on seed germination, the growth of immature
and mature seedlings, water status, and photosynthetic pigments was alleviated in transgenic seedlings. 相似文献