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1.
) 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》1999,124(4):3255
The Onychophora display a distinctive mechanism of feeding that involves the entanglement of prey in a sticky secretion. This secretion is produced in the slime glands and ejected as adhesive threads from a pair of oral papillae on either side of the head. Biochemical analyses of the secretion reveals it to be a composite material containing protein, sugar, lipid and a surfactant, nonylphenol. The identification of nonylphenol in the secretion is significant in that this is the first report of this compound from a natural source. The proteins are the principal component of the slime and the amino acid composition of the crude secretion suggests the presence of collagen or a ‘collagen-like’ domain. One or more of the high molecular weight proteins are O-glycosylated where the predominant modification is a single N-acetylgalactosamine (GalNAc). This study adds to our understanding of the chemical and biochemical composition of the unusual onycophoran slime gland secretion. 相似文献
2.
Myxococcus leaves a trail of slime on agar as it moves. A filament of slime can be seen attached to the end of a cell, but it is seen only at one end at any particular moment. To identify genes essential for A motility, transposon insertion mutations with defective A motility were studied. Fifteen of the 33 mutants had totally lost A motility. All these mutant cells had filaments of slime emerging from both ends, indicating that bipolar secretion prevents A motility. The remaining 18 A motility mutants, also produced by gene knockout, secreted slime only from one pole, but they swarmed at a lower rate than A(+) and are called 'partial' gliding mutants, or pgl. For each pgl mutant, the reduction in swarm expansion rate was directly proportional to the reduction in the coefficient of elasticotaxis. The pgl mutants have a normal reversal frequency and normal gliding speed when they move. But their probability of movement per unit time is lower than pgl(+) cells. Many of the pgl mutants are produced by transposon insertions in glycosyltransferase genes. It is proposed that these glycosyltransferases carry out the synthesis of a repeat unit polysaccharide that constitutes the slime. 相似文献
3.
Poulsen LR López-Marqués RL McDowell SC Okkeri J Licht D Schulz A Pomorski T Harper JF Palmgren MG 《The Plant cell》2008,20(3):658-676
Vesicle budding in eukaryotes depends on the activity of lipid translocases (P(4)-ATPases) that have been implicated in generating lipid asymmetry between the two leaflets of the membrane and in inducing membrane curvature. We show that Aminophospholipid ATPase3 (ALA3), a member of the P(4)-ATPase subfamily in Arabidopsis thaliana, localizes to the Golgi apparatus and that mutations of ALA3 result in impaired growth of roots and shoots. The growth defect is accompanied by failure of the root cap to release border cells involved in the secretion of molecules required for efficient root interaction with the environment, and ala3 mutants are devoid of the characteristic trans-Golgi proliferation of slime vesicles containing polysaccharides and enzymes for secretion. In yeast complementation experiments, ALA3 function requires interaction with members of a novel family of plant membrane-bound proteins, ALIS1 to ALIS5 (for ALA-Interacting Subunit), and in this host ALA3 and ALIS1 show strong affinity for each other. In planta, ALIS1, like ALA3, localizes to Golgi-like structures and is expressed in root peripheral columella cells. We propose that the ALIS1 protein is a beta-subunit of ALA3 and that this protein complex forms an important part of the Golgi machinery required for secretory processes during plant development. 相似文献
4.
Adaptation, oscillations and relay in a model for cAMP secretion in cellular slime molds 总被引:2,自引:0,他引:2
This work presents a unified theory for adaptation and for quiescence-excitable-oscillatory transitions in the cyclic AMP secretion system of the cellular slime mold amoeba Dictyostelium discoideum. 相似文献
5.
Summary The effect of monensin on polysaccharide slime secretion by root tips of corn (Zea mays) was studied. Various treatment times and ionophore concentrations were tested: none resulted in inhibition of slime secretion. Because monensin changes the pH of the medium, its effect was also monitored in strongly buffered media and at different pH's. Even in such media, monensin did not inhibit slime secretion. We also measured the effect of the drug after a pulse with [3H]fucose or a pulse followed by a chase. The amount of labeled slimed secreted was not altered by the ionophore. However, 10M monensin affected the development of root tips and drastically reduced their growth. We showed that monensin inhibits the secretion of -amylase by the scutellum of the same plantlet. The importance of the nature of the secretory compound in relation to monensin inhibition of its secretion is discussed.Abbrevations Hepes
N-2-hydroxyethylpiperazine-N-2-ethane-sul-fonic acid
- Mes
2-(N-morpholino)ethane-sulfonic acid 相似文献
6.
Motile microorganisms either swim, by using flagella or glide over surfaces by mechanisms that are poorly understood. In cyanobacteria, gliding motility appears as a relatively slow and smooth surface-associated translocation in the direction of the long axis of the filaments at rates up to a few micrometers a second. Many filamentous species translocate in a highly coordinated manner. Translational movements are usually accompanied by revolutions around the long axis of the filament. While moving, the cyanobacteria secrete slime which is left behind as a twisted and collapsed thin tube. The observation of the slime secretion process shows that the mucilage is formed as fine bands that emerge in close proximity to the cells cross walls. Ultrastructural studies have revealed that the cyanobacteria possess at their cross walls complex, pore-like organelles, which might be involved in slime secretion. As each cell possess two different sets of pores pointing in opposite direction, the coordinated activity of these structures could explain how the filament can reverse the direction of locomotion. Furthermore, ultrastructural studies have shown that rotating cyanobacteria possess cell surfaces formed by parallel, helically arranged surface fibrils. As the arrangement of these fibrils corresponds with the path of the filaments during locomotion, it might be imaginable that these fibrils serve as screw thread guiding the rotation of the filaments, with the necessary thrust for locomotion being derived from the secretion of slime using the pores at the cross walls. 相似文献
7.
Alexander Baer Ivo de Sena Oliveira Max Steinhagen Annette G. Beck‐Sickinger Georg Mayer 《Journal of Zoological Systematics and Evolutionary Research》2014,52(4):265-272
Onychophorans (velvet worms) use an adhesive, protein‐based slime secretion for prey capture and defence. The glue‐like slime is ejected via a pair of modified limbs and the sticky threads entangle the victim. In this study, we analysed the protein composition of slime in twelve species of Onychophora from different parts of the world, including two species of Peripatidae from Costa Rica and Brazil and ten species of Peripatopsidae from Australia, using sodium dodecyl sulphate polyacrylamide gel electrophoresis. Our results revealed high intraspecific conservation in protein composition of slime in each species studied. In contrast, the protein profiles differ considerably in both number and position of bands between the species. We observed the highest number of differences (in 20 of 33 considered band positions) between a peripatid and a peripatopsid species, whereas the lowest number of differences (in four band positions) occurs between two closely related egg‐laying species. The reconstructed maximum parsimony cladogram based on the electrophoretic characters largely reflects the phylogenetic relationships of the species studied, suggesting that the slime protein profiles contain useful phylogenetic information. Based on our findings, we suggest that the slime protein profiling is a valuable, non‐invasive method for identifying the onychophoran species. Moreover, this method might help to discover potentially new species of Onychophora, given that the ~200 described species most likely underrepresent the actual diversity of the group. 相似文献
8.
J. Kötters A. Prahst B. Skura H. Rosenthal E. A. Black J. Rodigues-Lopez 《Zeitschrift fur angewandte Ichthyologie》1997,13(1):1-8
On a fishing vessel and in the laboratory, trials were undertaken to study the effect of ozonation on rockfish bacteria ( Sebastes spp.) during transport from fishing grounds. At the beginning of the experiment, bacterial loads were the same on ozone-treated and control samples. Fish transported in ozonated water lost some of their bacterial film with surface slime when they were pumped from the boat's hold into the processing plant. Ozonation appears to encourage the detachment of the surface slime and bacterial film by partly oxidizing the excess slime excreted during capture. When ozonated intermittently during transport the fish shelf-life was extended by about 36 h. The same ozonation treatment was simulated in the laboratory which revealed higher bacterial counts on the ozonated fish than on the control samples. Fish used in this trial are believed to have been stored for several days prior to ozonation. This storage may have resulted in changes in the properties of the surface slime, counteracting the effect of ozonation. 相似文献
9.
The polysaccharide component contained in the slime of the causative agent of melioidosis was obtained. This component was found to be a biopolymer, mainly of the carbohydrate nature, consisting of galactose, glucose, mannose, rhamnose and two unidentified carbohydrates. The slime polysaccharide component contained two thermostable and acid-resistant antigens. The action of alkali led to the loss of one of these antigens. Rabbit antisera to the preparations of the slime polysaccharide component with titers of 1:64 to 1:256, determined in the immunodiffusion test, were obtained. In the precipitation test the slime polysaccharide component reacted with antisera from sick experimental animals, thus confirming the suggestion of its secretion in the process of the development of melioidosis infection. 相似文献
10.
E. Schnepf 《Planta》1972,103(4):334-339
Summary The secretion of the carbohydratous trapping slime of Drosophyllum is known to be mediated by the Golgi apparatus. In order to determine whether this secretion depends on the synthesis of new membrane material or runs for a long time by the cycling of membrane molecules, the influence of several inhibitors of protein synthesis was studied. Cycloheximide decreases the secretion of polysaccharides drastically, whereas puromycin and the amino acid analogues fluorophenylalanine and ethionine have little or no influence. An attempt is made to find explanations for these results, which, although contradictory, have parallels in the literature. 相似文献
11.
Kurt Zaar 《Archives of microbiology》1978,117(3):303-308
The myxomycetePhysarum polycephalum synthesizes copious amounts of slime when differentiating into the hard walled resting stage. The chemical composition of slime obtained after introduction of spherulation in a nutrient and a non-nutrient salt-medium has been analysed and compared. The composition of slime is almost identical after the two different induction methods. This slime could be labelled with radioactived-[U-14C]glucose,32PO4
3–,35SO4
2– and75SeO4
2–. The kinetics of slime secretion after both induction methods has been followed using different criteria. The sulfate analog75SeO4
2– seems to be incorporated into the slime, partially replacing sulfate groups of the sulfated polysaccharide. Furthermore,d-[U-14C]glucose was used to labe the spherule walls. Determination of an alkali resistent polysaccharide component serves as a new method to follow wall formation. 相似文献
12.
AN ARCHAIC SLIME MOULD IN BALTIC AMBER 总被引:1,自引:0,他引:1
Abstract: Several sporocarps of a slime mould are preserved on a plant fragment in a piece of Baltic amber (Tertiary, Eocene). The fossil is assigned to the extant genus Protophysarum (Myxomycota sensu stricto , Physarales) and described as P. balticum sp. nov. It is the third record of fossil sporocarps of a slime mould and the second record of the subclass Myxogasteromycetidae. All three records of fossil sporocarps are inclusions in Baltic amber. The find provides new insights regarding the occurrence and phylogeny of myxomycetes in the Palaeogene. 相似文献
13.
The digestive glands of Pinguicula vulgaris become fully maturewhilst still enclosed in the bud. All the gland cells remainintact on the fully expanded unstimulated leaves. As the secretoryhead cells mature, a special layer forms between the plasmalemmaand the cell wall. This layer is shown to be different fromthe typical labyrinthine wall of transfer cells and serves forthe storage of digestive enzymes. Ultrastructural analysis,including morphometry, indicates that the digestive enzymesare synthesized on the RER of the head cells and transferredinto the cell wall, particularly into the slime layer, and vacuoles.This transfer is achieved firstly through continuity of theendoplasmic reticulum with vacuoles (static) and the periplasmicspace (dynamic) and, secondly, into the latter through exocytosisof coated Golgi vesicles and of some vacuoles filled with enzymes. Pinguicula vulgaris L., carnivorous plant, digestive glands, ultrastructure, protein synthesis secretion 相似文献
14.
网柄细胞状黏菌是一类介于植物和动物之间的原生生物。尽管形态微小,但因为同时具有动物细胞和植物细胞的特点,且生命周期短暂易重复,故而对其进行生物学特性及应用的研究具有重要价值。本文从网柄细胞状黏菌的生活史循环、生物学特性、生态多样性、在医学和药物领域的探索及其与其他微生物关系等5个方面探讨网柄细胞状黏菌的生物学特性及应用的研究进展及意义,展望网柄细胞状黏菌未来在医学和生态等方面的研究前景及其潜在的应用价值,旨在为我国网柄细胞状黏菌同其他领域的交叉研究相结合提供视野,探索其在促进人类的科学进步、改善生活环境及攻克疾病方面的作用及意义。 相似文献
15.
Chemical Characterization of Polysaccharide from the Slime Layer of the Cyanobacterium Microcystis flos-aquae C3-40 总被引:7,自引:1,他引:6
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John L. Plude Dorothy L. Parker Olivia J. Schommer Robert J. Timmerman Stephanie A. Hagstrom James M. Joers Robert Hnasko 《Applied microbiology》1991,57(6):1696-1700
Macromolecular material from the slime layer of the cyanobacterium Microcystis flos-aquae C3-40 was defined as material that adhered to cells during centrifugation in growth medium but was dislodged by washing with deionized water and retained within dialysis tubing with a molecular-weight cutoff of 3,500. At each step of this isolation procedure, the slime was observed microscopically. Cells in the centrifugal pellet were surrounded by large amounts of slime that excluded negative stain, whereas cells that had been washed with water lacked visible slime. Two independently isolated lots of slime contained no detectable protein (<1%, wt/wt) and consisted predominantly of anthrone-reacting polysaccharide. Sugars in a hydrolysate of slime polysaccharide were derivatized with trimethylsilylimidazole and examined by gas chromatography-mass spectrometry. The composition of the slime polysaccharide was 1.5% (wt/wt) galactose, 2.0% glucose, 3.0% xylose, 5.0% mannose, 5.5% rhamnose, and 83% galacturonic acid. This composition resembles that of the plant polysaccharide pectin, which was treated in parallel as a control. Consistent with earlier indications that M. flos-aquae slime preferentially binds certain cations, the ratio of Fe to Na in the dialyzed slime was 104 times that in the growth medium. The composition of the slime is discussed with respect to possible mechanisms of cation binding in comparison with other cyanobacterial exopolysaccharides and pectin. 相似文献
16.
Telesheva LF Dolgushin II Korobeĭnikova EN 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2004,(3):90-92
The functional activity of neutrophils, peroxidation products and antioxidative activity of cervical secretion in women with the acute inflammatory process in the upper section of the reproductive tract were under study. The most pronounced changes in the function of neutrophils were observed in women with the low antioxidative activity of cervical slime. 相似文献
17.
Samuel H. Barondes Eric C. Beyer Wayne R. Springer Douglas N. Cooper 《Journal of cellular biochemistry》1981,16(3):233-242
Endogenous lectins in both cellular slime molds and chicken tissues have been localized primarily intracellularly, in contrast with the predominantly extracellular localization of the glycoproteins, glycolipids, and glycosaminoglycans with which they might interact. Here we present evidence that lectins in both of these organisms may be externalized and become associated with the cell surface and/or extracellular materials. In chicken intestine, chicken-lactose-lectin-II is shown to be localized in the secretory granules of the goblet cells, along with mucin, and to be secreted onto the intestinal surface. In embryonic muscle, chicken-lactose-lectin-I is shown to be externalized with differentiation, ultimately becoming localized on the surface of myotubes and in the extracellular spaces. In a cellular slime mold, Dictyostelium purpureum, externalization of lectin is elicited by either polyvalent glycoproteins that bind the small amount of endogenous cell surface lectin, or by slime mold or plant lectins that bind unoccupied complementary cell surface oligosaccharides. These results suggest that externalization of endogenous lectin may be a response to specific external signals. We conclude that lectins are frequently held in intracellular reserves awaiting release for specific external functions. 相似文献
18.
Strains ofPseudomonas aeruginosa recovered from pulmonary infections in cystic fibrosis (CF) patients are often mucoid in appearance owing to the secretion of a viscous slime exopolysaccharide (EPS). Unlike most mucoid isolates, strains WcM#2, P10, and P11 produce mucoid colonies after 24 h of incubation at 37°C, which become nonmucoid upon further incubation; this suggests the presence of a slime-degrading enzyme or depolymerase. Using both qualitative and quantitative assays, the presence of a slime EPS depolymerase was confirmed in each of these three strains as well as in four of four additional mucoid strains. Depolymerase activity was lower but still detectable in four of four nonmucoid strains. Enzyme preparations from strains WcM#2, P10, and P11 were active on most, but not all, slime EPS preparations fromP. aeruginosa strains, as well as sodium alginate; greater activity was observed on substrates after deacetylation. Comparisons are made between the enzyme described in this study and previous reports of slime EPS depolymerase in mucoid strains ofP. aeruginosa. 相似文献
19.
Wolgemuth CW 《Biophysical journal》2005,89(2):945-950
Myxococcus xanthus is a common Gram-negative bacterium that moves by a process called gliding motility. In myxobacteria, two distinct mechanisms for gliding have been discovered. S-type motility requires the extension, attachment, and retraction of type IV pili. The other mechanism, designated as A-type motility, may be driven by the secretion and swelling of slime; however, experiments to confirm or refute this model are still lacking and the force exerted by this mechanism has not been measured. A previously published experiment found that when an M. xanthus cell became stuck at one end, the cell underwent flailing motions. Based on this experiment, I propose an elastic model that can estimate the force produced by the A-motility engine and the bending modulus of a single myxobacterial cell. The model estimates a bending modulus of 3 x 10(-14) erg cm and a force between 50-150 pN. This force is comparable to that predicted by slime extrusion, and the bending modulus is 30-fold smaller than that measured in Bacillus subtilis. This model suggests experiments that can further quantify this process. 相似文献
20.
Onychophorans use a unique hunting and defense strategy, which involves the ejection of an adhesive slime secretion produced by a pair of specialized glands. So far, a comparative study on the anatomy of these glands has not been carried out among different species. In this article, we compare anatomical features of slime glands in representatives of two major onychophoran subgroups, the Peripatopsidae and the Peripatidae, from different parts of the world. Our data show that the musculature of the reservoir is conserved whereas the composition of the secretory duct displays taxon‐specific variation. Major differences concern the arrangement of glandular endpieces, which are distributed along the duct in Peripatopsidae but condensed in numerous repeated rosettes in Peripatidae. In addition, there are differences in the attachment pattern of slime glands to the inner surface of the body wall and to the outer surface of the gut between the two major onychophoran subgroups. A tube‐like structure with a putative valve‐like function is found at the transition of the secretory duct and the reservoir in the five Peripatopsidae species studied whereas it is absent in the two representatives of Peripatidae. Our findings suggest that the arrangement of musculature in the reservoir of the slime gland has remained unchanged since the divergence of Peripatidae and Peripatopsidae, while the composition of the secretory duct has been altered in one of these groups. However, the direction of evolutionary changes in duct composition cannot be determined unambiguously due to current uncertainty regarding the phylogenetic relationships of Onychophora. J. Morphol. 2012. © 2012 Wiley Periodicals, Inc. 相似文献