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1.
Yang J  Bromage TG  Zhao Q  Xu BH  Gao WL  Tian HF  Tang HJ  Liu DW  Zhao XQ 《PloS one》2011,6(6):e19833

Background

Environmental stress can accelerate the directional selection and evolutionary rate of specific stress-response proteins to bring about new or altered functions, enhancing an organism''s fitness to challenging environments. Plateau pika (Ochotona curzoniae), an endemic and keystone species on Qinghai-Tibetan Plateau, is a high hypoxia and low temperature tolerant mammal with high resting metabolic rate and non-shivering thermogenesis to cope in this harsh plateau environment. Leptin is a key hormone related to how these animals regulate energy homeostasis. Previous molecular evolutionary analysis helped to generate the hypothesis that adaptive evolution of plateau pika leptin may be driven by cold stress.

Methodology/Principal Findings

To test the hypothesis, recombinant pika leptin was first purified. The thermogenic characteristics of C57BL/6J mice injected with pika leptin under warm (23±1°C) and cold (5±1°C) acclimation is investigated. Expression levels of genes regulating adaptive thermogenesis in brown adipose tissue and the hypothalamus are compared between pika leptin and human leptin treatment, suggesting that pika leptin has adaptively and functionally evolved. Our results show that pika leptin regulates energy homeostasis via reduced food intake and increased energy expenditure under both warm and cold conditions. Compared with human leptin, pika leptin demonstrates a superior induced capacity for adaptive thermogenesis, which is reflected in a more enhanced β-oxidation, mitochondrial biogenesis and heat production. Moreover, leptin treatment combined with cold stimulation has a significant synergistic effect on adaptive thermogenesis, more so than is observed with a single cold exposure or single leptin treatment.

Conclusions/Significance

These findings support the hypothesis that cold stress has driven the functional evolution of plateau pika leptin as an ecological adaptation to the Qinghai-Tibetan Plateau.  相似文献   

2.
Yingzhong Y  Yue C  Guoen J  Zhenzhong B  Lan M  Haixia Y  Rili G 《Gene》2007,403(1-2):118-124
Hemoglobin (Hb) plays an important role in oxygen transfer from lung to tissues. Possession of a Hb with high oxygen affinity helps highland animals to adapt to high altitude, has been studied profoundly. Plateau pika (Ochotona curzoniae), a native species living at 3,000-5,000 m above sea level on Qinghai-Tibet Plateau, is a typical hypoxia and low temperature tolerant mammal. To investigate the possible mechanisms of plateau pika Hb in adaptation to high altitude, the complete cDNA and amino acid sequences of plateau pika hemoglobin alpha and beta chains have been described. Compared with human Hb, alterations in important regions can be noted: alpha111 Ala-->Asn, beta35 Tyr-->Phe, beta112 Cys-->Val, beta115 Ala-->Ser, and beta125 Pro-->Gln. Phylogenetic analysis of alpha and beta chains shows that plateau pika is closer to rabbit than to other species. This study provides essential information for elucidating the possible roles of hemoglobin in adaptation to extremely high altitude in plateau pika.  相似文献   

3.
4.
低氧性肺血管收缩反应钝化是高原鼠兔适应低氧环境的重要策略,但参与该生理代偿反应的功能基因尚不明确。间隙连接蛋白40 (Connexin40, Cx40) 在哺乳动物肺血管内皮表达。本研究对生活在海拔3 200 m的高原鼠兔进行28 d模拟海拔5 000 m低氧处理,以Sprague Dawley (SD) 大鼠为对照,采用免疫组化法分析模拟低氧处理后高原鼠兔和SD大鼠肺组织形态结构,qPCR和蛋白印记法检测Cx40基因和蛋白表达量变化,探究Cx40在高原鼠兔低氧性肺血管收缩反应钝化中的潜在作用。结果显示,低氧处理后,高原鼠兔肺泡呈空泡囊状,Cx40蛋白在支气管和肺血管中均表达,Cx40 基因mRNA水平随着低氧暴露而升高,但其蛋白质水平呈下降趋势,肺支气管Cx40蛋白无明显变化。SD大鼠肺血管和肺支气管表达的Cx40蛋白均无明显变化。暗示生活在高海拔低氧环境中的高原鼠兔,Cx40蛋白下调可抑制血管收缩信号,减弱低氧性肺血管收缩反应,使低氧性肺血管收缩反应钝化,以适应高原缺氧环境。研究结果可为高原土著动物适应高寒缺氧环境提供基础理论数据。  相似文献   

5.
Wang Z  Zhang Y 《Gene》2012,501(2):206-212
Erythropoietin (EPO) is a glycoprotein hormone, expressed mainly in fetus liver and adult kidneys. EPO plays an important role in enhancing red blood cell formation in bone marrow under hypoxia. Plateau zokor (Myospalax baileyi), an subterranean burrowing endemic rodent inhabiting areas of 2 800-4 200 m above sea level on Qinghai-Tibet Plateau, is a typical high hypoxia tolerant mammal with high ratio of oxygen utilization in adaptation to the harsh plateau environment. To investigate the possible mechanisms of adaptation of plateau zokor EPO to high altitude, the complete cDNA and amino acid sequences of plateau zokor EPO have been described. Phylogenetic tree of Epo showed the convergence of the Spalax and Myospalax, indicating that, the convergent evolution was driven by similar hypoxic ecological niches. Our results showed that some common sites under positive selection in zokor (116M and 144A) and Spalax (102R, 116M, 144A and 152P) are the important sites for Epo biological activity. This study thus reports a gene level observation which may be involved in adaptation to underground life at high altitude.  相似文献   

6.
Yang J  Wang ZL  Zhao XQ  Wang de P  Qi de L  Xu BH  Ren YH  Tian HF 《PloS one》2008,3(1):e1472

Background

Environmental stress can accelerate the evolutionary rate of specific stress-response proteins and create new functions specialized for different environments, enhancing an organism''s fitness to stressful environments. Pikas (order Lagomorpha), endemic, non-hibernating mammals in the modern Holarctic Region, live in cold regions at either high altitudes or high latitudes and have a maximum distribution of species diversification confined to the Qinghai-Tibet Plateau. Variations in energy metabolism are remarkable for them living in cold environments. Leptin, an adipocyte-derived hormone, plays important roles in energy homeostasis.

Methodology/Principal Findings

To examine the extent of leptin variations within the Ochotona family, we cloned the entire coding sequence of pika leptin from 6 species in two regions (Qinghai-Tibet Plateau and Inner Mongolia steppe in China) and the leptin sequences of plateau pikas (O. curzonia) from different altitudes on Qinghai-Tibet Plateau. We carried out both DNA and amino acid sequence analyses in molecular evolution and compared modeled spatial structures. Our results show that positive selection (PS) acts on pika leptin, while nine PS sites located within the functionally significant segment 85-119 of leptin and one unique motif appeared only in pika lineages-the ATP synthase α and β subunit signature site. To reveal the environmental factors affecting sequence evolution of pika leptin, relative rate test was performed in pikas from different altitudes. Stepwise multiple regression shows that temperature is significantly and negatively correlated with the rates of non-synonymous substitution (Ka) and amino acid substitution (Aa), whereas altitude does not significantly affect synonymous substitution (Ks), Ka and Aa.

Conclusions/Significance

Our findings support the viewpoint that adaptive evolution may occur in pika leptin, which may play important roles in pikas'' ecological adaptation to extreme environmental stress. We speculate that cold, and probably not hypoxia, may be the primary environmental factor for driving adaptive evolution of pika leptin.  相似文献   

7.
高原鼠兔低氧诱导因子- 1α的初步研究   总被引:3,自引:0,他引:3  
Hypoxia-induced factor-1 plays a key role during the cell hypoxia trausduction. Hypoxia induced factor-1α (HIF-1α) is a functional subunit of hypoxia-indueed factor-1. Plateau pika ( Ochotona curron/ae), which is a Qinghai-Tibet plateau native animal lived above 3 000 m, has high ratio of oxygen utilization to adapt to plateau hypoxia environments. One fragment of the coding re-gion of eDNA sequence of plateau pika HIF-1α was obtained by RT-PCR technique using a degeneracy PCR primer based on previ-ously reported eDNA sequence in human, cattle, house mouse and Norway rat HIF-1α gene. It was directly inserted into the vector pMD18-T. DNA sequencing proved that it was highly homology with human (91%), cattle (91%), house mouse (89%) and Nor-way rat 89%) HIF-1α gene. The study provides basic important information for the HIF-1α cDNA whole sequence cloning of pla-teau pika and its functional study.  相似文献   

8.
低氧性肺血管收缩反应钝化(blunted Hypoxic Pulmonary Vasoconstriction, bHPV)是高原鼠兔在进化过程中形成的对低氧环境的一种适应性策略。以胎儿期、幼龄期及成年期高原鼠兔和昆明白小鼠为研究对象,对两者的肺组织进行发育生物学、比较组织学和比较组织化学研究,以探讨高原鼠兔bHPV形成的组织形态学基础。结果表明:(1)高原鼠兔各时期肺组织微血管的双层弹性纤维含量均低于25%,无明显肌化现象;(2)高原鼠兔直径10~50 μm、50~100 μm和100~200 μm微血管的中膜肌层厚度与其外径比(MT/ED)随年龄增加而逐渐下降;成年高原鼠兔的肺组织微血管MT/ED10-50显著低于成年昆明白小鼠(P < 0.05),但MT/ED50-100和MT/ED100-200均无明显的种间差异(P > 0.05);(3)在直径10~50 μm直径范围微血管中,高原鼠兔和昆明白小鼠平滑肌细胞数与内皮细胞数的比值(SMC/EC)均随年龄增长呈上升趋势,且高原鼠兔SMC/EC的比值极显著低于同年龄组的昆明白小鼠(P < 0.01)。导致高原鼠兔bHPV发生的组织学基础可能是其肺组织中直径10~50μm微血管平滑肌细胞数量少。  相似文献   

9.
瘦素(leptin)由ob 基因编码,对调节能量代谢起着重要作用。本研究建立了高原鼠兔瘦素的原核表达系统,并对其进行了原核表达。研究中作者从高原鼠兔瘦素基因的cDNA 文库中,扩增编码高原鼠兔瘦素的核酸序列,并利用DNA 基因重组技术将其克隆到原核表达载体pET30a(+ )中,构建了高原鼠兔瘦素原核表达载体pET30a(+ )/ ppleptin。对目的片段进行测序确认后,将其转化到大肠杆菌BL21 中,并利用IPTG 诱导外源性目的蛋白表达。表达的包涵体蛋白经溶解及变性后上柱纯化。重组质粒经测序检测后,表明原核载体构建正确。同时,SDS - PAGE 凝胶电泳结果显示,重组菌在16KD 处有明显新增条带,纯化后的目的蛋白条带纯度较高。该结果为高原鼠兔瘦素的后续基础研究提供了基础资料。  相似文献   

10.
Dispersal is an important aspect in organism's life history which could influence the rate and outcome of evolution of organism. Plateau pika is the keystone species in community of grasslands in Tibetan Plateau. In this study, we combine genetic and field data to character the population genetic pattern and dispersal dynamics in plateau pika (Ochotona curzoniae). Totally, 1,352 individual samples were collected, and 10 microsatellite loci were analyzed. Results revealed that plateau pika possessed high genetic diversity and inbreeding coefficient in a fine‐scale population. Dispersal distance is short and restricted in about 20 m. An effective sex‐biased dispersal strategy is employed by plateau pika: males disperse in breeding period for mating while females do it after reproduction for offspring and resource. Inbreeding avoiding was shown as the common driving force of dispersal, together with the other two factors, environment and resource. In addition, natal dispersal is female biased. More detailed genetic analyzes are needed to confirm the role of inbreeding avoidance and resource competition as ultimate cause of dispersal patterns in plateau pika.  相似文献   

11.
瘦素(Leptin)蛋白是调节机体能量代谢的关键因子之一。前期研究显示高原鼠兔Leptin蛋白发生了适应性进化。功能实验表明,在温暖或寒冷条件下高原鼠兔Leptin通过减少食物摄取和增加能量消耗调节能量平衡,显示了其调节适应性产热过程的潜力。本研究以高原鼠兔Leptin cDNA为模板扩增高原鼠兔obese (ob)基因编码区序列504 bp,改造并构建哺乳动物真核细胞乳腺特异表达载体pBC1-lep,同时通过组织块法原代培养建立奶山羊乳腺上皮细胞系,并通过pBC1-lep质粒脂质体法转染及转基因细胞的筛选,成功获得转染Leptin的阳性细胞。本研究为利用转基因动物实现奶山羊乳腺中特异表达高原鼠兔Leptin提供了一条可能的途径,完成了乳腺特异真核表达载体的构建。  相似文献   

12.
低氧对雄性高原鼠兔性腺的影响   总被引:2,自引:0,他引:2  
在人工模拟低氧环境下(低压舱模拟5000m和7000m海拔高度),低氧暴露24h和7d,观察低氧对受试动物性腺的影响。结果表明,急性低氧24h,高原鼠兔血浆雌二醇(E2)明显升高;低氧暴露7d,高原鼠兔血浆E2仍维持一较高水平;5000m低氧暴露7d,其睾丸指数无明显变化,7000m时却有所降低。同等条件下,大鼠睾丸指数明显增高;5000m和7000m低氧暴露7d对高原鼠兔睾丸组织形态无明显影响,然而,大鼠曲细精管间隙增大,且曲细精管内各级细胞排列紊乱。低氧环境下,高原鼠兔雄体血浆E2增高,可能是其低氧适应的特征之一  相似文献   

13.
Despite their important roles in host nutrition, metabolism and adaptability, the knowledge on how the mammalian gut microbial community assemble is relatively scanty, especially regarding the ecological mechanisms that govern microbiota along environmental gradients. To address this, we surveyed the diversity, function and ecological processes of gut microbiota in the wild plateau pika, Ochotona curzoniae, along the elevational gradient from 3106 to 4331 m on ‘the Roof of the World’—Qinghai-Tibet Plateau. The results indicated that the alpha, beta and functional diversity of gut microbiota significantly increased with elevation, and elevation significantly explained the variations in the gut microbial communities, even after controlling for geographical distance, host sex and body weight. Some gene functions (e.g. nitrogen metabolism and protein kinases) associated with metabolism were enriched in the high-altitude pikas. Null model and phylogenetic analysis suggest that the relative contributions of environmental filtering responsible for local gut communities increased with elevation. In addition, deterministic processes dominated gut microbial communities in the high-altitude (more than 3694 m) pikas, while the percentages of stochastic and deterministic processes were very close in the low-altitude (3106 and 3580 m) pikas. The observed mechanisms that influence pika gut microbiota assembly and function seemed to be mainly mediated by the internal gut environment and by the external environmental pressure (i.e. lower temperature) in the harsh high-altitude environment. These findings enhance our understanding of gut microbiota assembly patterns and function in wild mammals from extreme harsh environments.  相似文献   

14.
青藏高原高寒草原生态系统是我国特有的生态系统类型,由于受到人为破坏的影响,目前该地区草原生态系统功能退化,优良牧草减少,有毒植物蔓延。高原鼠兔(Ochotona curzoniae)和高原鼢鼠(Myospalax baileyi)是青藏高原东缘高寒草原中最重要的两种小型哺乳动物,其采食行为和挖掘洞穴的生活特性必然对生态系统产生影响,但其与有毒植物之间的互作关系尚未揭示。基于此,在甘肃省玛曲县河曲马场自然生长的高寒草原生态系统中开展了有毒植物的分布与高原鼠兔、高原鼢鼠之间的相关性研究。结果表明,该高寒草原生态系统中分布有毒植物27种,分属于菊科、豆科、毛茛科等11科。在此基础上,测定了该地区有毒植物的生物多样性指数、均匀度指数和丰富度指数,并探究了单位面积条件下有毒植物的分布特征与高原鼠兔和高原鼢鼠种群密度之间的相关性,发现该地区高寒草原有毒植物的蔓延与高原鼠兔的密度之间存在密切的负相关关系(P0.05),而与高原鼢鼠的相关性不显著(P0.05)。  相似文献   

15.
高原鼠兔的血象及其与低氧适应的关系   总被引:7,自引:0,他引:7  
高原鼠兔是新开发的实验动物,能很好地适应高原低氧环境。本研究报道了驯化高原鼠兔的部分血液指标,并与野生高原鼠兔及部分其它实验动物和人的相应指标作了比较。同时对高原鼠兔能适应高原低氧环境的机理作了探讨。高原鼠兔红细胞数量高、体积小、红细胞膜表面积大,从而提高了Hb的利用率,使尽可能多的Hb参与摄氧和释氧。  相似文献   

16.
β多样性反映生物群落沿某一环境梯度的物种周转速率, 该研究尝试采用β多样性揭示植物群落随小型啮齿草食动物干扰梯度变化的生态过程。该研究利用野外随机样地的采集数据, 分析了高原鼠兔(Ochotona curzoniae)不同干扰强度下Whittaker指数的变化特征, 并利用群落二元丰富度的方差分解法, 确定了单个物种(SCBD)和单个干扰位点(LCBD)对β多样性的贡献。主要结果: 随高原鼠兔干扰强度增加, 植物群落内物种周转速率呈先增加后降低的趋势; 占据位点数居中的物种对区域内的β多样性贡献较大, 其中冰草(Agropyron cristatum)、臭蒿(Artemisia hedinii)、小花草玉梅(Anemone rivularis var. flore-minore)等单个物种对整个区域内β多样性的贡献最为突出; 整个区域内干扰位点T0 (高原鼠兔干扰强度为0)对区域β多样性贡献值最大, LCBD值和该位点的群落丰富度呈显著负相关关系, 但与高原鼠兔干扰强度无显著关联。说明重点保护LCBD值高的干扰位点所在的高寒草甸, 以及SCBD值较高的冰草、臭蒿、小花草玉梅, 对保护高原鼠兔存在时高寒草甸植物群落多样性具有重要意义。  相似文献   

17.
Luo Y  Gao W  Gao Y  Tang S  Huang Q  Tan X  Chen J  Huang T 《Mitochondrion》2008,8(5-6):352-357
Pikas originated in Asia and are small lagomorphs native to cold climates. The plateau pika, Ochotona curzoniae is a keystone species on the Qinghai-Tibet Plateau and an ideal animal model for hypoxic adaptation studies. Altered mitochondrial function, especially cytochrome c oxidase activity, is an important factor in modulation of energy generation and expenditure during cold and hypoxia adaptation. In this study, we determined the complete nucleotide sequence of the O. curzoniae mitochondrial genome. The plateau pika mitochondrial DNA is 17,131bp long and encodes the complete set of 37 proteins typical for vertebrates. Phylogenetic analysis based on concatenated heavy-strand encoded protein-coding genes revealed that pikas are closer to rabbit and hare than to rat. This suggests that rabbit or hare would be a good control animal for pikas in cold and hypoxia adaptation studies. Fifteen novel mitochondrial DNA-encoded amino acid changes were identified in the pikas, including three in the subunits of cytochrome c oxidase. These amino acid substitutions potentially function in modulation of mitochondrial complexes and electron transport efficiency during cold and hypoxia adaptation.  相似文献   

18.
The effects of hypoxia on the levels of essential macroelements and trace elements (K, Na, Ca, Mg, Cu, Zn, Fe, and Mn) in the heart muscles of Wistar rats and plateau pikas (Ochotona curzoniae) were studied by atomic absorption spectrometry. Unlike the rat, the plateau pika is tolerant to hypoxia. The levels of K, Na, and the trace element Mn were not significantly changed in rat or pika hearts after exposure to hypoxia for 1, 10, or 25 d at simulated altitudes of 5000 and 7000 m. Other minerals (Ca, Mg, Cu, Zn, and Fe) were significantly affected by hypoxia and the levels followed different time-courses under different hypoxic regimes in these two animals. There were marked differences between the rat and pika in myocardial accumulation of essential elements such as Ca, which was increased to high levels in the rat but not affected in the pika. The results suggest that hypoxia affects animal physiological mechanisms by regulating the levels of essential elements.  相似文献   

19.
高原鼠兔血清无机元素含量的分析   总被引:1,自引:0,他引:1  
测定分析了高原鼠兔血清电解质、无机元素、血清铁和总铁结合力, 并与人和其它实验动物的相应指标进行了比较。得出以下主要结论: 1.高原鼠兔血清电解质浓度不受生活环境和食物条件的影响, 其机体能自身调节血清离子浓度, 以维持电解质在血液中的相对衡定; 2.高原鼠兔血清铁含量与其栖息地海拔相关, 随海拔升高血清铁含量增加; 3.高原鼠兔通过增加运铁蛋白与铁的结合力作为适应高原低氧环境的方式之一。  相似文献   

20.
高原鼢鼠(Myospalax baileyi)是青藏高原特有的地下鼠,其地下洞道严重缺氧。一般来说,低氧会促进细胞凋亡。为了探讨高原鼢鼠适应低氧环境的分子机制,本文应用生物信息学方法对p53下游凋亡促进基因Pidd、Fas、Bax、Puma、Apaf-1、Scotin、Perp、Igfbp3和凋亡抑制基因Bcl-2的序列和编码的氨基酸序列进行了进化分析,并以SD大鼠(Rattus norvegicus)为对照,研究了这些基因在不同海拔环境条件下(3 300 m和2 260 m)的表达模式。结果表明:(1)高原鼢鼠细胞凋亡基因的序列与以色列鼹鼠(Nannospalax galili)同源性最高;预测的PIDD、PUMA、Apaf-1、IGFBP3和BCL-2编码蛋白结构域与以色列鼹鼠的存在明显的趋同进化位点;SIFT评估发现,高原鼢鼠和以色列鼹鼠与其他物种相比,p53、PIDD、PUMA、Apaf-1和IGFBP3氨基酸序列分别在78、853、157、320和285号位点的变异对其功能有显著影响;(2)在高海拔条件下(3 300 m),高原鼢鼠肺组织中凋亡促进基因Pidd、Bax、Puma和Apaf-1表达水平显著下降,凋亡抑制基因Bcl-2表达水平显著升高,而在SD大鼠中凋亡促进基因和凋亡抑制基因的表达水平均没有变化;高原鼢鼠中Bcl-2/Bax比值随海拔的升高显著上升,而在SD大鼠中没有变化。以上结果提示,高原鼢鼠p53结构变异可能导致其下游基因表达模式与SD大鼠不同,其中凋亡促进基因Pidd、Bax、Puma和Apaf-1表达水平下降,凋亡抑制基因Bcl-2表达水平上升,从而抑制了细胞在低氧条件下的凋亡;在长期低氧的作用下,高原鼢鼠p53下游基因产物PIDD、PUMA、Apaf-1和IGFBP3产生了影响其功能的变异位点,这可能改变了它们与发挥功能的复合物的结合力,从而抑制了细胞凋亡。因此,通过长期的低氧适应,高原鼢鼠肺组织中与细胞凋亡相关的基因产物结构发生变异,导致其基因表达水平发生变化,从而抑制细胞凋亡,这是高原鼢鼠适应地下低氧洞道生境的分子机制之一。  相似文献   

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