共查询到20条相似文献,搜索用时 15 毫秒
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James E. Klaunig Randall J. Ruch Peter J. Goldblatt 《In vitro cellular & developmental biology. Plant》1985,21(4):221-228
Summary Rainbow trout (Salmo gairdneri) hepatocytes were isolated using a two-step perfusion through the portal vein. A typical perfusion yielded 2.92×106 liver cells with a mean viability of 96.3%. Hepatocytes comprised 93.4% of the total cell isolate. Survival of hepatocytes
in suspension culture was dependent on fetal bovine serum concentration and temperature of incubation. Serum concentrations
of 5, 10, and 20% produced the highest survival during primary culture. Hepatocyte survival was in inverse proportion to the
incubation temperature. Trout hepatocyte DNA synthesis and mitosis decreased during the culture period. Cytochromep
450 activity decreased rapidly during the first 2 d of culture and then remained low but measurable during the remaining 8 d
of culture. Culture temperature also influenced thep
450 activity with lower temperatures producing greater activity. Morphologic changes occurred in the cells during culture. Isolated
hepatocytes self-aggregated, forming strands and clumps that increased in size with time in culture. Junctional complexes
between cells were evident within the aggregates. Nuclear atypia, increases in size and number of autophagic vacuoles, and
the appearance of bundles of intermediate filaments also were observed with increased time in culture.
This work was supported in part by an American Cancer Society Grant (Ohio Division, Inc.) and an NIH Biomedical Research Support
Grant 5507RR05700010. 相似文献
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Gillian Cowlishaw 《The Australian journal of anthropology》2010,21(2):208-227
In western Sydney, I found an extreme version of what I propose is a national Aboriginal mythopoeia, that is, a powerful system of beliefs and practices in relation to Aboriginal people and culture. A reified Aboriginal culture is promoted at institutional sites and in reconciliation discourses that evokes the presence of something precious and mysterious that must be re‐read into local Aboriginal people, but which assiduously avoids their actual circumstances and subjectivities. The awkward relationships and avoidances evident in a western Sydney reconciliation group are posited as a benign example of this mythology, born of a ‘sentimental politics’ of regret and reparation at work in Australia. Unity between the state and civil society is evident here, thus requiring an analysis that goes beyond a critique of government policies and programmes as intentional and rational, and grasps the nature of the widespread desire for Aboriginality. Through ethnographic attention to the actual relationships of Indigenous people and others, anthropologists can avoid being complicit in this regressive, separatist construction. 相似文献
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《Environmental Biology of Fishes》1976,1(1):107-107
Book reviews
Marine ecology and fisheriesD. H. Cushing. 1975. Cambridge Univ. Press, Cambridge. 278 pp. 相似文献7.
Gillian Cowlishaw 《The Australian journal of anthropology》2011,22(2):170-188
This second part of Mythologising Culture examines the responses of Aboriginal people in western Sydney to the valorizing of Aboriginality by the Australian nation. As Aboriginal culture has become the object of restitution, regret and reconciliation, Aboriginal people are being called upon to represent and produce Culture in iconic forms such as painting, dancing and other performances and representations. These activities, often depicted as remedial, are seldom initiated and controlled by Aboriginal people, but rather disturb cultural adjustments and established social relations. I discuss a range of responses elicited by these post‐colonial activities, from enthusiastic embrace to the rejection of state sponsored culture. Further, I consider the structural implications of suburban Aborigines being offered the chance to distance themselves from the anonymous suburban poor. 相似文献
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The submerged culture of mammalian cells; the spinner culture 总被引:48,自引:0,他引:48
McLIMANS WF DAVIS EV GLOVER FL RAKE GW 《Journal of immunology (Baltimore, Md. : 1950)》1957,79(5):428-433
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Summary Transient experiments were performed during a batch fermentation ofCorynebacterium glutamicum ATCC 14296 in which the flow of air to the reactor was cut off. The fluorescence of the culture was observed to vary linearly with the culture redox potential during the transient. This observation was made below the sensitivity limit of a dissolved oxygen probe. It appears that redox potential in conjunction with fluorescence measurements are of utility in studying extra- and intracellular reduction states, particularly in the microaerobic regime. 相似文献
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近自然纯培养法对细菌培养的初步研究 总被引:2,自引:0,他引:2
依据微生物在自然生境中协作生长的基本特性,提出一种对传统纯培养技术的改进思路及方法:设计一种有孔培养皿,皿内覆盖有不允许细菌通过、但营养物质可以自由流动的微孔滤膜。培养时将该培养皿放入被分离微生物所需生境中,可以克服传统纯培养难以提供外源活性物质的缺陷,一定程度上弥补了混合培养法和传统纯培养法的弱点,从而达到增强部分微生物可培养性、甚至培养出未培养微生物的目的。 相似文献
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Summary A mist bioreactor system for the plant tissue cultures was developed. Using this system, the growth of Dianthus caryophyllus multiple shoots was directly measured. Tissue growth in mist bioreactor system was far better than that on agar medium and almost comparable to that in liquid medium. The mass increase (final dry weight/initial dry weight) in the mist culture was 2.85 while 3.28 in the liquid flask culture. Shoots were seriously vitrified in flask culture but these vitrifications could be considerably cured by using the mist culture system. 相似文献
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Embryo-like structures (ELS) with clearly defined cotyledons and radicles have been regenerated fromLupinus albus L. microspores. ELS induction from microspores in liquid medium was successful, with a maximum of over 3,500 ELS per anther being produced from microspores predominantly at the early binucleate stage of development. Cytological analysis revealed that first pollen grain mitosis occurred in closed buds with maximum ELS production being obtained from buds at the point of first petal emergence. Generally there was a lack of synchronisation of microspores within anthers from the less mature bud germination from ELS has not been observed; recurrent somatic embryogenesis occurred following internal cleavage within the ELS or on the surface of the ELS. Methods of increasing the level of mature ELS capable of germination are under investigation.Abbreviations BA
6-benzyladenine
- 24-d
2,4-dichlorophenoxyacetic acid
- NAA
naphthaleneacetic acid
- 2iP
N-isopentyl-adenine
- GA3
gibberellic acid
- S&H
Schenk & Hildebrandt medium (1972)
- M&S
Murashige & Skoog medium (1962)
- N&N
Nitsch & Nitsch medium (1969)
- B5
Gamborg's B5 medium (1968)
- NNB5
N&N salts plus B5 vitamins
- ELS
Embryo Like Structures
- DAPI
4, 6-Diamidino-2-Phenylindole 相似文献
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Craig Hadley 《American journal of physical anthropology》2007,132(1):160-161
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Yasunari Ogihara 《Journal of plant research》1979,92(3):163-171
Effects of three different auxins and kinetin in various combinations on greening and redifferentiation of the callus ofHaworthia setata were investigated. All auxins at the concentration of 50 mg/l inhibited callus greening. NAA in combination with kinetin promoted both callus greening and production of redifferentiated
shoots. Low concentrations of IAA without kinetin promoted redifferentiation of shoots, but not callus greening. Addition
of 2,4-D completely inhibited both greening and redifferentiation regardless of the level of kinetin except for the effects
on shoot formation in the medium with 0.1 mg/l 2,4-D added. The calluses with the highest chlorophyll content were observed in the medium containing 2.0 mg/l kinetin without any auxins or with 0.1 mg/l NAA added. Most frequent shoot redifferentiation was observed in the medium containing 0.1 mg/l IAA without kinetin (redifferentiation rate; 67%), followed by the medium containing 10 mg/l NAA with 2.0 mg/l kinetin (44%), and 0.1 mg/l 2,4-D with 0.2 mg/l kinetin (33%). Generally, higher degrees of greening were associated with better growth. However, the auxins (IAA, NAA and
2,4-D) given at concentrations optimal for growth did not exhibit the highest degree of callus greening. Differences of the
three auxins in their actions and interaction with kinetin were disclosed.
Contribution from the Laboratory of Genetics, Faculty of Agriculture, Kyoto University, Japan, No. 423 相似文献
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The use of fed-batch procedures offers distinct advantages over other modes of operation of bioreactors, and is a widely researched technique. These advantages are discussed; some uses of fed-batch procedures and the associated methods of modelling and control are reviewed. 相似文献
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