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1.
L. R. Blinks 《The Journal of general physiology》1930,14(1):139-162
Electrical resistance and polarization were measured during the passage of direct current across a single layer of protoplasm in the cells of Valonia ventricosa impaled upon capillaries. These were correlated with five stages of the P.D. existing naturally across the protoplasm, as follows: 1. A stage of shock after impalement, when the P.D. drops from 5 mv. to zero and then slowly recovers. There is very little effective resistance in the protoplasm, and polarization is slight. 2. The stage of recovery and normal P.D., with values from 8 to 25 mv. (inside positive). The average is 15 mv. At first there is little or no polarization when small potentials are applied in either direction across the protoplasm, nor when very large currents pass outward (from sap to sea water). But when the positive current passes inward there is a sudden response at a critical applied potential ranging from 0.5 to 2.0 volts. The resistance then apparently rises as much as 10,000 ohms in some cases, and the rise occurs more quickly in succeeding applications after the first. When the potential is removed there is a back E.M.F. displayed. Later there is also an effect of such inward currents which persists into the first succeeding outward flow, causing a brief polarization at the first application of the reverse potential. Still later this polarization occurs at every exposure, and at increasingly lower values of applied potentials. Finally there is a "constant" state reached in which the polarization occurs with currents of either direction, and the apparent resistance is nearly uniform over a considerable range of applied potential. 3. A state of increased P.D.; to 100 mv. (inside positive) in artificial sap; and to 35 or 40 mv. in dilute sea water or 0.6 M MgSO4. The polarization response and apparent resistance are at first about as in sea water, but later decrease. 4. A reversed P.D., to 50 mv. (outside positive) produced by a variety of causes, especially by dilute sea water, and also by large flows of current in either direction. This stage is temporary and the cells promptly recover from it. While it persists the polarization appears to be much greater to outward currents than to inward. This can largely be ascribed to the reduction of the reversed P.D. 5. Disappearance of P.D. caused by death, and various toxic agents. The resistance and polarization of the protoplasm are negligible. The back E.M.F. of polarization is shown to account largely for the apparent resistance of the protoplasm. Its calculation from the observed resistance rises gives values up to 150 mv. in the early stages of recovery, and later values of 50 to 75 mv. in the "constant" state. These are compared with the back E.M.F. similarly calculated from the apparent resistance of intact cells. The electrical capacitance of the protoplasm is shown by the time curves to be of the order of 1 microfarad per cm.2 of surface. 相似文献
2.
The protoplasm of Valonia macrophysa forms a delicate layer, only a few microns in thickness, which contains numerous chloroplasts and nuclei. The outer surface is in contact with the cell wall, the inner with the vacuolar sap. As far as microscopic observation goes, these two surfaces seem alike; but measurements of potential difference indicate that they are decidedly different. We find that the chain sap | protoplasm | sap gives about 14.5 millivolts, the inner surface being positive to the outer. In order to explain this we may assume that the protoplasm consists of layers, the outer surface, X, differing from the inner surface, Y, and from the body of the protoplasm, W. We should then have the unsymmetrical chain sap | X | W | Y | sap which could produce an electromotive force. If the two surfaces of such a very thin layer of protoplasm can be different, it is of fundamental significance for the theory of the nature of living matter. 相似文献
3.
The direct current longitudinal resistance of the squid giant axon was measured as a function of the electrode separation. Large sea water electrodes were used and the inter-electrode length was immersed in oil. The slope of the resistance vs. separation curve is large for a small electrode separation, but becomes smaller and finally constant as the separation is increased. An analysis of the resistance vs. length curves gives the following results. The nerve membrane has a resistance of about 1000 ohm cm.2 The protoplasm has a specific resistance of about 1.4 times that of sea water. The resistance of the connective tissue sheath outside the fiber corresponds to a layer of sea water about 20µ in thickness. The characteristic length for the axon is about 2.3 mm. in oil and 6.0 mm. in sea water. 相似文献
4.
Treatment of Nitella with distilled water apparently removes from the cell something which is responsible for the normal irritability and the potassium effect, (i.e. the large P.D. between a spot in contact with 0.01 M KCl and one in contact with 0.01 M NaCl). Presumably this substance (called R) is partially removed from the protoplasm by the distilled water. When this has happened a pinch which forces sap out into the protoplasm can restore its normal behavior. The treatment with distilled water which removes the potassium effect from the outer protoplasmic surface does not seem to affect the inner protoplasmic surface in the same way since the latter retains the outwardly directed potential which is apparently due to the potassium in the sap. But the inner surface appears to be affected in such fashion as to prevent the increase in its permeability which is necessary for the production of an action current. The pinch restores its normal behavior, presumably by forcing R from the sap into the protoplasm. 相似文献
5.
W. J. V. Osterhout 《The Journal of general physiology》1944,28(1):17-22
The vacuolar surface of Nitella is covered with a non-aqueous film too thin to be visible as a separate membrane. The motion of the protoplasm may subject this film to a good deal of mechanical disturbance. Apparently this does not rupture the film for no dye escapes into the protoplasm as the result of such disturbance when the vacuolar sap is deeply stained with neutral red or brilliant cresyl blue. When the deeply stained central vacuole breaks up into several smaller vacuoles, leaving the outer protoplasmic surface in its normal position, there is no evidence of the escape of dye into the protoplasm through the film surrounding the vacuole. 相似文献
6.
Electrical properties of Nitella (Tolypella) cells were studied,with special reference to their electric resistance The resistanceof the protoplasm, as well as the potential of the inside ofthe cell, was measured by inserting capillary electrode intothe cell, which had been sealed with vaseline externally atthe middle so that the current across the protoplasm could bereadily determined. The characteristics of the rectification and impedance weremeasured The electric current was found to flow across the protoplasmiclayer more easily from the sap to the external water than inthe reverse direction. The resistance and the capacitance ofthe cell varied with the a.c frequencies used for the measurement Simultaneous measurements were made of the action potential,the action current, and the resistance change of the protoplasmin order to determine the relationship between them An intimateparallelism between the time courses of these three phenomenawas discovered. An attempt was made to interpret the resultsby taking into account the dissociation of ions in the protoplasm.
1Present address: Japan Atomic Energy Research Institute, Tokai,Ibaraki Prefecture. (Received May 8, 1962; ) 相似文献
7.
E. B. Damon 《The Journal of general physiology》1929,13(2):207-221
In measurements of P.D. across the protoplasm in single cells, the presence of parallel circuits along the cell wall may cause serious difficulty. This is particularly the case with marine algae, such as Valonia, where the cell wall is imbibed with a highly conducting solution (sea water), and hence has low electrical resistance. In potential measurements on such material, it is undesirable to use methods in which the surface of the cell is brought in contact with more than one solution at a time. The effect of a second solution wetting a part of the cell surface is discussed, and demonstrated by experiment. From further measurements with improved technique, we find that the value previously reported for the P.D. of the chain Valonia sap | Valonia protoplasm | Valonia sap is too low, and also that the P.D. undergoes characteristic changes during experiments lasting several hours. The maximum P.D. observed is usually between 25 and 35 mv., but occasionally higher values (up to 82 mv.) are found. The appearance of the cells several days after the experiment, and the P.D.''s which they give with sea water, indicate that no permanent injury has been received as a result of exposure to artificial sap. If such cells are used in a second measurement with artificial sap, however, the form of the P.D.-time curve indicates that the cells have undergone an alteration which persists for a long time. On the basis of the theory of protoplasmic layers, an attempt has been made to explain the observed changes in P.D. with time, assuming that these changes are due to penetration of KCl into the main body of the protoplasm. 相似文献
8.
OSTERHOUT WJ 《The Journal of general physiology》1956,39(6):963-976
The behavior of the cell depends to a large extent on the permeability of the outer non-aqueous surface layer of the protoplasm. This layer is immiscible with water but may be quite permeable to it. It seems possible that a reversible increase or decrease in permeability may be due to a corresponding increase or decrease in the water content of the non-aqueous surface layer. Irreversible increase in permeability need not be due primarily to increase in the water content of the surface layer but may be caused chiefly by changes in the protoplasm on which the surface layer rests. It may include desiccation, precipitation, and other alterations. An artificial cell is described in which the outer protoplasmic surface layer is represented by a layer of guaiacol on one side of which is a solution of KOH + KCl representing the external medium and on the other side is a solution of CO2 representing the protoplasm. The K+ unites with guaiacol and diffuses across to the artificial protoplasm where its concentration becomes higher than in the external solution. The guaiacol molecule thus acts as a carrier molecule which transports K+ from the external medium across the protoplasmic surface. The outer part of the protoplasm may contain relatively few potassium ions so that the outwardly directed potential at the outer protoplasmic surface may be small but the inner part of the protoplasm may contain more potassium ions. This may happen when potassium enters in combination with carrier molecules which do not completely dissociate until they reach the vacuole. Injury and recovery from injury may be studied by measuring the movements of water into and out of the cell. Metabolism by producing CO2 and other acids may lower the pH and cause local shrinkage of the protoplasm which may lead to protoplasmic motion. Antagonism between Na+ and Ca++ appears to be due to the fact that in solutions of NaCl the surface layer takes up an excessive amount of water and this may be prevented by the addition of suitable amounts of CaCl2. In Nitella the outer non-aqueous surface layer may be rendered irreversibly permeable by sharply bending the cell without permanent damage to the inner non-aqueous surface layer surrounding the vacuole. The formation of contractile vacuoles may be imitated in non-living systems. An extract of the sperm of the marine worm Nereis which contains a highly surface-active substance can cause the egg to divide. It seems possible that this substance may affect the surface layer of the egg and cause it to take up water. A surface-active substance has been found in all the seminal fluids examined including those of trout, rooster, bull, and man. Duponol which is highly surface-active causes the protoplasm of Spirogyra to take up water and finally dissolve but it can be restored to the gel state by treatment with Lugol solution (KI + I). The transition from gel to sol and back again can be repeated many times in succession. The behavior of water in the surface layer of the protoplasm presents important problems which deserve careful examination. 相似文献
9.
A method distinguishing between the concentration effect due to the cell wall and that due to the protoplasm is described: the importance of this lies in the fact that if the protoplasm shows a concentration effect one or both ions of the salt must tend to enter its outer surface. Studies on the concentration effect of KCl with living protoplasm of Nitella show that when P.D. is plotted as ordinates and the logarithm of concentration as abscissæ the graph is not the straight line demanded in the ideal case by theory but has less slope and is somewhat concave to the axis of the abscissæ. With a variety of salts the dilute solution is positive, which indicates that the cation has a greater mobility in the protoplasm than the anion or that the partition coefficient of the cation (Ac) increases faster than that of the anion (Aa) as the concentration increases. If the result depended on the partition coefficients we should say that when Ac ÷ Aa increases with concentration the dilute solution is positive. When Ac ÷ Aa decreases as the concentration increases the dilute solution is negative. In either case the increase in concentration may be accompanied by an increase or by a decrease in the relative amount of salt taken up. Theoretically therefore there need be no relation between the sign of the dilute solution and the relative amount of salt taken up with increasing concentration. Hypothetical diagrams of the electrical conditions in the cell are given. If we define the chemical effect as the P.D. observed in leading off at two points with equivalent concentrations of different salts we may say that the chemical effect of the protoplasm is very much greater than that of the cell wall. 相似文献
10.
(a) Photosynthesis with protoplasm isolated from Chara or Nitella as measured by C14 fixation has been obtained at a rate 12 to 15 per cent of that of the whole cells. (b) Photosynthesis by cut cells of Chara or Nitella with the vacuolar sap removed was at a rate comparable to that of the whole cells. (c) Both the protoplasm and the cut cells reduced CO2 in the light to sucrose and hexose phosphates. Other products formed were also detected by paper chromatography. In contrast, dark controls fixed the C14 into products associated with plant respiration. (d) An important difference in the products from the extruded protoplasm was the absence of C14-labelled pentoses or sedoheptulose which were formed, however, by the whole or cut cells. This suggests that the most sensitive site affected by disruption of the cells may be the steps involved in the regeneration of the "C-2 acceptor" for CO2 fixation in photosynthesis. 相似文献
11.
L. R. Blinks 《The Journal of general physiology》1932,16(1):147-156
The potential difference across the protoplasm of impaled cells of two American species of Halicystis is compared. The mean value for H. Osterhoutii is 68.4 mv.; that for H. ovalis is 79.7 mv., the sea water being positive to the sap in both. The higher potential of H. ovalis is apparently due to the higher concentration of KCl (0.3 M) in its vacuolar sap. When the KCl content of H. Osterhoutii sap (normally 0.01 M or less) is experimentally raised to 0.3 M, the potential rises to values about equal to those in H. ovalis. The external application of solutions high in potassium temporarily lowers the potential of both, probably by the high mobility of K+ ions. But a large potential is soon regained, representing the characteristic potential of the protoplasm. This is about 20 mv. lower than in sea water. The accumulation of KCl in the sap of H. ovalis is apparently not due to the higher mobility of K+ ion in its protoplasm, since the electrical effects of potassium are practically identical in H. Osterhoutii, where KCl is not accumulated. 相似文献
12.
E. B. Damon 《The Journal of general physiology》1932,16(2):375-395
The P.D. across the protoplasm of Valonia macrophysa has been studied while the cells were exposed to artificial solutions resembling sea water in which the concentration of KCl was varied from 0 to 0.500 mol per liter. The P.D. across the protoplasm is decreased by lowering and increased by raising the concentration of KCl in the external solution. Changes in P.D. with time when the cell is treated with KCl-rich sea water resemble those observed with cells exposed to Valonia sap. Varying the reaction of natural sea water from pH 5 to pH 10 has no appreciable effect on the P.D. across Valonia protoplasm. Similarly, varying the pH of KCl-rich sea water within these limits does not alter the height of the first maximum in the P.D.-time curve. The subsequent behavior of the P.D., however, is considerably affected by the pH of the KCl-rich sea water. These changes in the shape of the P.D.-time curve have been interpreted as indicating that potassium enters Valonia protoplasm more rapidly from alkaline than from acidified KCl-rich sea water. This conclusion is discussed in relation to certain theories which have been proposed to explain the accumulation of KCl in Valonia sap. The initial rise in P.D. when a Valonia cell is transferred from natural sea water to KCl-rich sea water has been correlated with the concentrations of KCl in the sea waters. It is assumed that the observed P.D. change represents a diffusion potential in the external surface layer of the protoplasm, where the relative mobilities of ions may be supposed to differ greatly from their values in water. Starting with either Planck''s or Henderson''s formula, an equation has been derived which expresses satisfactorily the observed relationship between P.D. change and concentration of KCl. The constants of this equation are interpreted as the relative mobilities of K+, Na+, and Cl- in the outer surface layer of the protoplasm. The apparent relative mobility of K+ has been calculated by inserting in this equation the values for the relative mobilities of Na+ (0.20) and Cl- (1.00) determined from earlier measurements of concentration effect with natural sea water. The average value for the relative mobility of K+ is found to be about 20. The relative mobility may vary considerably among different individual cells, and sometimes also in the same individual under different conditions. Calculation of the observed P.D. changes as phase-boundary potentials proved unsatisfactory. 相似文献
13.
Marian Irwin 《The Journal of general physiology》1929,12(3):407-418
The rate of diffusion through the non-aqueous layer of the protoplasm depends largely on the partition coefficients mentioned above. Since these cannot be determined we have employed an artificial system in which chloroform is used in place of the non-aqueous layer of the protoplasm. The partition coefficients may be roughly determined by shaking up the aqueous solutions with chloroform and analyzing with the spectrophotometer (which is necessary with methylene blue because we are dealing with mixtures). This will show what dyes may be expected to pass through the protoplasm into the vacuole in case it behaves like the artificial system. From these results we may conclude that the artificial system and the living cell act almost alike toward methylene blue and azure B, which supports the notion of non-aqueous layers in the protoplasm. There is a close resemblance between Valonia and the artificial system in their behavior toward these dyes at pH 9.5. In the case of Nitella, on the other hand, with methylene blue solution at pH 9.2 the sap in the artificial system takes up relatively more azure B (absorption maximum at 650 mµ) than the vacuole of the living cell (655 mµ). But both take up azure B much more rapidly than methylene blue. A comparison cannot be made between the behavior of the artificial system and that of the living cell at pH 5.5 since in the latter case there arises a question of injury to cells before enough dye is collected in the sap for analysis. 相似文献
14.
W. C. Cooper Jr. M. J. Dorcas W. J. V. Osterhout 《The Journal of general physiology》1929,12(3):427-433
The entrance of strong electrolytes into Valonia is very slow unless the cells are injured. This, together with the very high electrical resistance of the protoplasm, suggests that they may penetrate largely as undissociated molecules formed at the surface of the protoplasm by the collision of ions. Under favorable circumstances KCl may be absorbed to the extent of 3 x 10–8 mols per hour per sq. cm. of surface together with about 0.17 as much NaCl. Other substances which seem to penetrate to some extent are Li, Rb, Br, BrO3, I, IO3, and selenite. Little or no penetration is shown by SCN, ferricyanide, ferrocyanide, formate, salicylate, tungstate, seleniate, NO2, SO3, Sb, glycerophosphate, and many heavy metals and the alkaline earths. In sea water whose specific gravity had been increased by CsCl cells of Valonia floated for over a year and there was little or no penetration of Cs except as the result of injury. The penetration of NH4Cl decreases the specific gravity of the sap and causes the cells to float: under these circumstances they live indefinitely. It is probable that NH3 or NH4OH penetrates and is subsequently changed to NH4Cl. It would seem that if the sea contained a little more ammonia this would be a floating organism. 相似文献
15.
Yoshio Hayashi 《Journal of theoretical biology》1980,85(3):451-467
A mathematical model of protoplasmic streaming in a plant cell such as Nitella and Chara is studied. General rheological equations for the non-Newtonian fluid is derived theoretically, and the boundary value problem for the model is solved. The pattern of motion of cytoplasm in a living cell is obtained, and the rheological property of protoplasm is evaluated in vivo. 相似文献
16.
Using multinucleate cells of Nitella 2 or 3 inches in length it is possible to kill one end with chloroform without producing at the other any immediate alteration which can be detected by our present methods. When a spot in external contact with sap is killed its potential difference falls approximately to zero and it is therefore possible to measure the potential difference across the protoplasm at any desired point merely by leading off from that point to the one where the protoplasm has been killed. The results indicate that the inner and outer protoplasmic surfaces differ, for when both surfaces are in contact with the same solution (cell sap) there is an electromotive force of about 15.9 millivolts, the inner surface being positive to the outer (i.e. the positive current tends to flow from the inner surface through the electrometer to the outer surface). The situation resembles that in Valonia where the corresponding value (with Valonia sap applied to the outside) has been reported as about 14.5 millivolt (the inner surface being positive to the outer). It would seem appropriate to designate this as radial polarity. 相似文献
17.
S. C. Brooks 《The Journal of general physiology》1925,7(3):327-330
The electrical conductivity of the plasmodium of the slime-mold Brefeldia maxima (Fr.) Rost., which constitutes practically pure protoplasm, was found to be approximately equivalent under normal conditions to that of a 0.00145 N NaCl solution, and about 2.8 times that of the liquid in contact with which it developed. When bathed in 1 per cent sea water, the conductivity was much increased, becoming greater than that of the surrounding fluid. These preliminary tests are in agreement with the supposition that the protoplasm is permeable to and in equilibrium with its environment in so far as electrolytes are concerned. 相似文献
18.
Twenty-four-hour induction of freezing and drought tolerance in plumules of winter rye seedlings by desiccation stress at room temperature in the dark 总被引:4,自引:3,他引:1
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Exposure of seedlings of winter rye (Secale cereale L., cv. Puma) for 2 weeks or 24 hours to desiccation stress (40% relative humidity) at room temperature (21°C) in the dark induced degrees of freezing and drought tolerance in the plumules comparable to those produced by cold conditioning for 2 weeks at 3°C. The induction was associated with repression of growth and could not be produced in plumules excised from the seedlings indicating a requirement for translocation of nutrients from the endosperm. Rapid increase in osmotic pressure, soluble proteins, and phospholipids in plumules in association with the development of freezing and drought tolerance and the requirement of endosperm suggested diversion of nutrient from use in extension growth, to use in augmentation of protoplasm in plumule cells. Since cold acclimation slowed or arrested growth and is associated with augmentation of protoplasm, it is suggested that the common element in the induction of freezing tolerance by cold and drought is the necessity for producing a condition of augmented protoplasm and membranes in cells thus reinforcing a similar conclusion reached from seasonal studies on woody plants. 相似文献
19.
L. R. Blinks 《The Journal of general physiology》1929,13(2):223-229
The cells of Halicystis impaled on capillaries reach a steady P.D. of 60 to 80 millivolts across the protoplasm from sap to sea water. The outer surface of the protoplasm is positive in the electrometer to the inner surface. The P.D. is reduced by contact with sap and balanced NaCl-CaCl2 mixtures; it is abolished completely in solutions of NaCl, CaCl2, KCl, MgSO4, and MgCl2. There is prompt recovery of P.D. in sea water after these exposures. 相似文献
20.
E. B. Damon 《The Journal of general physiology》1938,21(3):383-409
The concentration effect with sea waters containing more than the normal amount of potassium has been studied in Valonia macrophysa. This was done by comparing the initial changes in P.D. across the protoplasm when natural sea water bathing the cell was replaced by various isotonic dilutions of KCl-rich sea waters. With small dilutions of KCl-rich sea waters, the P.D.-time curves are of the same form as with the undiluted solution, exhibiting the fluctuations characteristic of KCl-rich solutions. This indicates that with these solutions K+ enters Valonia protoplasm and plays an important part in the P.D. The value of the initial rise in P.D. decreases with increasing dilution. With high dilutions of KCl-rich sea waters, the P.D.-time curves are of quite different shape, resembling the curves with diluted natural sea water; the P.D. is practically independent of small changes in the concentration of potassium, and increases with increasing dilution. That is, with these higher dilutions, the sign of the concentration effect is reversed, becoming the same as with diluted natural sea water. The greater the concentration of KCl in the undiluted sea water, the higher is the critical dilution at which K+ ceases to influence the P.D. For a wide range of sea waters containing both KCl and NaCl, it is shown that the concentration effect above the critical dilution is determined solely by the activity of NaCl in the external solution. It is concluded that with dilute natural sea water and with high dilutions of KCl-rich sea waters we have to do with a diffusion potential, involving only the Na+ and Cl- ions, which are diffusing out from the vacuole. A quantitative relation between the composition of the sea water and the critical dilution has been deduced from the classical theory of the diffusion of electrolytes. It is shown that with dilutions less than this critical value the diffusion of K+ in the outer non-aqueous layer of the protoplasm is directed inward; hence K+ enters the protoplasm from these solutions. With dilutions greater than the critical value, the diffusion of K+ in this layer is directed outward; hence K+ does not enter the protoplasm. Since the P.D. shows no evidence of this outward diffusion of K+, it is concluded that the amount of K+ ordinarily present in the protoplasm is too small to produce any lasting electrical effect, and that the outward diffusion of K+ from the vacuole is prevented by the mechanism responsible for the accumulation of KCl in the cell sap. 相似文献