共查询到20条相似文献,搜索用时 15 毫秒
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Hsu LM 《Biochimica et biophysica acta》2002,1577(2):191-207
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Assembly and disassembly of the Drosophila RNA polymerase II complex during transcription. 总被引:11,自引:0,他引:11
J T Kadonaga 《The Journal of biological chemistry》1990,265(5):2624-2631
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Our laboratory has developed methods for transient state kinetic analysis of human RNA polymerase II elongation. In these studies, multiple conformations of the RNA polymerase II elongation complex were revealed by their distinct elongation potential and differing dependence on nucleoside triphosphate substrate. Among these are conformations that appear to correspond to different translocation states of the DNA template and RNA-DNA hybrid. Using alpha-amanitin as a dynamic probe of the RNA polymerase II mechanism, we show that the most highly poised conformation of the elongation complex, which we interpreted previously as the posttranslocated state, is selectively resistant to inhibition with alpha-amanitin. Because initially resistant elongation complexes form only a single phosphodiester bond before being rendered inactive in the following bond addition cycle, alpha-amanitin inhibits elongation at each translocation step. 相似文献
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