Genotype–environment interactions affect flower and fruit herbivory and plant chemistry of Arabidopsis thaliana in a transplant experiment

Large differences exist in flower and fruit herbivory between dune and inland populations of plants of Arabidopsis thaliana (Brassicaceae). Two specialist weevils Ceutorhynchus atomus and C. contractus (Curculionidae) and their larvae are responsible for this pattern in herbivory. We test, by means of a reciprocal transplant experiment, whether these differences reflect environmental influences or genetic variation in plant defense level. All plants suffered more damage after being transplanted to the dune site than after being transplanted to the inland site. Plants of inland origin suffered more flower and fruit herbivory than plants of dune origin when grown at the dune transplant site, but differences were much smaller at the inland site. Both flower damage by adult weevils and fruit damage by their larvae were subject to significant genotype × environment interactions. The observed pattern in herbivory is a strong indication for local adaption of plant defense to the level of herbivory by Ceutorhynchus. In order to identify the mechanism of defense, a quantitative analysis of glucosinolates was performed on the seeds with HPLC. Highly significant differences were found in glucosinolate types and total concentration. These patterns were mainly determined by the origin of the plants (dune or inland) and by a genotype × environment interaction. Herbivory was not significantly correlated to the concentration of glucosinolates in seeds. We therefore analyzed the total metabolic composition of seeds, using NMR spectroscopy and multivariate data analysis. Major differences in chemical composition were found in the water–methanol fractions: more glucosinolate and sucrose in the dune and more fatty acids, lipids and sinapoylmalate in the inland populations. We discuss which of these chemical factors could explain the marked differences in damage between populations.     

Parthenocarpic fruits in wild parsnip: Decoy defence against a specialist herbivore

Summary Parthenocarpy, the production of fruits without viable seeds, is a widespread phenomenon in plants. While failure to effect pollination or fertilization is often cited as the cause of parthenocarpy, this explanation alone is inadequate to explain why plants produce, maintain and further develop fruits. Wild parsnips (Pastinaca sativa) frequently produce parthenocarpic fruit. When parsnip webworms (Depressaria pastinacella), specialist feeders on wild parsnip, were given choices between normal fruit and parthenocarpic fruit, they exhibited a strong preference for parthenocarpic fruit. However, on parthenocarpic fruit, insects fed less efficiently and grew more slowly than insects fed normal fruit. Parthenocarpic fruits, then, may act as decoys that divert herbivores away from fruits that contain plant offspring.     

Effect of the stem blight pathogen, Phomopsis emicis, and the weevil, Perapion antiquum, on the weed Emex australis

The stem blight pathogen, Phomopsis emicis, and the weevil, Perapion antiquum, are two potential biological control agents for the annual weed, Emex australis. Neither pathogen nor weevil affected the development of rosette (5-wk-old) E. australis, but both significantly reduced stem length and number of new fruits in mature plants (10-wk-old) in growth room experiments. Stems grew two-thirds less than controls in plants attacked by weevils, had no net increase when both weevils and fungus were present, and collapsed in the presence of the fungus alone. Attack by weevils elicited a host response that slowed the development of the disease. Phomopsis emicis and Perapion antiquum reduced the ability of E. australis to produce new fruits by 77% and 68%, respectively. Pathogen and weevil together reduced the number of new fruits by 83%. Perapion antiquum did not carry or provide infection sites for P. emicis. The lack of damage by pathogen and weevil to rosettes could compromise their effectiveness as biological control agents.     

Gender, season and habitat: Patterns of variation in photosynthetic activity, growth and fecundity in Thymelaea velutina

Changes in the ecophysiological performance of a plant species due to different environmental conditions generally reflect adaptations to the habitat where the plant grows and are often related to its survival capacity in a particular place. We examined this with the dioecious shrub Thymelaea velutina, in two contrasting populations representing the extremes of the altitudinal gradient where the species lives (coastal dunes and mountain habitats over 1000 m). We measured net photosynthetic rates and stomatal conductance, estimated the level of plant stress by chlorophyll fluorescence, and assessed their correlations with growth rate, plant size, flower production and fruit set. We hypothesized that plants at high altitude were more photosynthetically stressed than at sea level and expected a gender × habitat interaction in performance as females need more resources than males. Plants in the mountain experienced chronic photoinhibition during winter and a reduced photosynthetic performance both in winter and spring compared to plants in coastal dunes. However, there was no association between any of the fluorescence variables and either plant growth or fecundity, suggesting that other factors are involved determining performance. Mountain plants showed also an apparent lower capacity of heat dissipation to excessive radiation than dune plants. In the dunes, the greater leaf area and mass can lead to a higher photosynthetic carbon gain by whole individuals compared to plants in the mountain. No effect of gender was detected on the ecophysiological performance of this species, which we partly attribute to the small size of fruits of the female plants.     

Community homogenization and the invasiveness of commensal species in Mediterranean afforested landscapes

The ecological consequences of homogenization remain relatively unexplored. One example of landscape-homogenizing is the establishment of plantations. We studied the effect of human-made forests by contrasting plant and small-mammal community composition between planted tree stands and adjacent natural habitat in two different Mediterranean habitats in Israel: (1) inland habitat where we focused on pine (Pinus halepensis) and carob (Ceratonia siliqua) stands, and (2) coastal sand dune habitat where we focused on planted acacia (Acacia saligna) stands. We first wanted to verify whether planted trees modify plant species composition, and second, if and how the small-mammal community is affected by the different habitat conditions created in plantations with different canopy cover. We were especially interested in the abundance of the commensal house mouse (Mus musculus). All tree stands underwent biotic homogenization indicated by abundance of house mice coupled with lower diversity of indigenous vegetation and small-mammal abundances and diversities. Habitat structural diversity was positively related with small-mammals diversity and was lower in artificial tree stands in both habitats. Our results suggest that using the abundance of commensal generalist species such as the house mouse relative to other more specialist small-mammals is a good approach to determine ecosystem integrity. Pre-commercial thinning treatment is a potential management tool to maintain a proportion of native tree species within the canopy of planted tree stands. However, until sufficient data is available for making generalizations, the exact level of thinning necessary to reverse the homogenization processes in man-made plantations and keeping indigenous small-mammal communities diverse and less prone to invasion must be determined empirically.     

版纳植物园龙脑香科植物迁地保护成效与应用探讨

龙脑香科植物广泛分布于热带亚洲,被认为是亚洲热带雨林的标志性物种.中国科学院西双版纳热带植物园从建园开始就注重龙脑香科植物的研究、收集与保护,并建立了龙脑香科植物专类园.经过几十年收集,现已成为我国龙脑香科植物重要的保育基地.该文结合历年的引种保育、物候及生长量观测等资料,系统整理了版纳植物园在龙脑香科植物引种保育和研...     

The effect of invasive plant management on the rate of removal of vertebrate-dispersed fruits

Chrysanthemoides monilifera ssp. rotundata is an invasive plant in eastern Australia. It produces large quantities of fleshy fruits, which are dispersed by birds and mammals, and has the potential to alter ecological processes in native plant communities in several ways. Seed dispersal of native plants growing amongst C. monilifera could be decreased (if C. monilifera is effective in competing for limited seed dispersers), increased (if C. monilifera-infested vegetation supports more seed dispersers), or be unchanged (if there is sufficient separation in fruiting time or dispersal agents between the species, or if dispersers are not limiting). We compared the rates of removal of fruits of native plants (Monotoca elliptica and Elaeocarpus spp.) from experimental stations in C. monilifera stands, in uninfested coastal vegetation, and in vegetation from which C. monilifera had been eliminated with herbicide. The rate of removal of fruits differed between plant species and locations, but not between any of the habitat treatments. This may have resulted from temporal separation of peak fruit availability between M. elliptica and C. monilifera, and differences in fruit morphology and disperser assemblages between Elaeocarpus spp. and C. monilifera. We also measured removal of C. monilifera fruits from stations in C. monilifera stands and from where C. monilifera had been eliminated with herbicide. In the herbicide-treated habitat, removal of C. monilifera fruits was reduced. This may assist control efforts and suggests that, to reduce seed dispersal, dense (core) populations should be targeted in control programs.     

The effect of the mirid bug Capsodes infuscatus on fruit production of the geophyte Asphodelus ramosus in a desert habitat

The effect of feeding of the mirid bug Capsodes infuscatus on fruit production of the geophyte Asphodelus ramosus was studied in a desert area in Israel. Plant and bug densities and percent loss of fruit production were measured in a relatively dry lower colluvial slope, an intermediate upper colluvial slope, and a relatively mesic wadi. Overall damage levels were very high, with 100% loss of fruit production in many plants. Within each habitat, the number of nymphs per plant clone was positively correlated with the number of ramets per clone and percent damage was positively correlated with number of nymphs per clone. However, percent damage was not correlated with number of plants per clone in any habitat. Although damage did significantly increase with plant density in the slope habitats, mean damage to fruit production per clone was lowest (50%) in the wadi where Asphodelus density was highest. As new ramets are tightly interwoven with their mother plants, occupation of new microsites depends on establishment of new clones from seeds. Therefore, the strong and density-dependent reduction in fruit production inflicted by Capsodes on the Asphodelus population on the slope has the potential to regulate the plant density in this habitat.     

Comparison of physical and chemical characters of tomato fruits grown from plants received from seeds or obtained in vitro

Results of experiments concerning comparison of tomato fruit properties which were collected from plants obtained in three manners are described. Control plants were received from seeds. Remaining plants were derived in vitro from leaf and shoot fragments on MS medium supplemented with IAA 0.2 mg·l⁻¹ and BA 2 mg·l⁻¹ (Górecka and Krzyżanowska 1991, Górecka et al. 1994) or with Fari’s et al. (1992) method of obtaining plants by decapitation of sterile seedlings and culture on MS medium without hormones. Evaluation of physical and chemical fruit characters was performed. In the spring experiment the biggest diameter (72 mm), weight (154 g) and volumne (151 ml) were characterized to fruits from plants obtained in vitro on MS medium with IAA and BA. Also fruits from plants received by Fari’s methods were significantly superior in these characters over fruits from the control plants. The fruits from the plants obtained in vitro on MS medium with IAA and BA had highest sugar content (2.95 % f.wt.) and fruits from in vitro plants after Fari’s method contained highest vit.C-13.4 mg·100 g⁻¹ f.wt (significant differences in comparison to control fruits). In other characters fruits from in vitro did not differ as compared to control ones. In the autumn experiments significant differences among fruit groups and characters evaluated were not stated. Generally, yield quality was poorer in the all autumn treatments.     

Comparing the effects of the exotic cactus-feeding moth, Cactoblastis cactorum (Berg) (Lepidoptera: Pyralidae) and the native cactus-feeding moth, Melitara prodenialis (Walker) (Lepidoptera: Pyralidae) on two species of Florida Opuntia

This study examined the effects of the native cactus moth borer, Melitara prodenialis, and the invasive cactus moth borer, Cactoblastis cactorum, on two common cactus species, Opuntia stricta and O. humifusa at coastal and inland locations in central Florida. Opuntia stricta were present only at coastal sites and O. humifusa were present at coastal and inland sites. Throughout the duration of the study, coastal plants were subject to damage solely by C. cactorum and inland plants solely by M. prodenialis. Results showed marginally significantly higher numbers of eggsticks on O. stricta than O. humifusa and significantly higher numbers at coastal sites than at inland sites. There was also significantly higher moth damage on O. stricta than O. humifusa and at coastal sites than inland sites, but not significantly so. However, there was a higher level of plant mortality for O. humifusa than for O. stricta and a significantly higher level of cactus mortality at inland sites when compared to coastal sites. This increased mortality may be due to increased attack by true bugs, Chelinidea vittiger, and by Dactylopius sp., combined with attack by M. prodenialis. Inland plants also tended to be smaller than coastal plants and could be more susceptible to the combined effects of all insects. Further long-term research on coastal cactus survival when attacked and unattacked by Cactoblastis is necessary to fully determine the effects of this moth on Opuntia survival.     

Habitat fragmentation and population features differently affect fruit predation,fecundity and offspring performance in a non‐specialist gypsum plant

• The effects of habitat fragmentation on plant populations are complex, as it might disrupt many ecological processes, including plant reproduction and plant–animal interactions. Gypsum specialist plants may be resilient to fragmentation due to their evolutionary history in fragmented landscapes, but the effects on non‐specialist plants occurring in gypsum are unknown.
• We conducted a study focusing on different aspects of the reproductive cycle of Astragalus incanus subsp. incanus, a plant facultatively linked to gypsum soils. We focused on plant fecundity and pre‐dispersal predation, obtained from field observations, and offspring performance, assessed in a common garden. Beyond fragment size and connectivity, we also considered habitat quality, population size and density and plant size as predictors.
• Fragment size and connectivity had no effect on plant fecundity, but jointly determined fruit predation, while fragment size was positively related to offspring growth. Population density, rather than population size, had a positive effect on predation but negatively affected plant fecundity and offspring performance. Habitat quality reduced both plant fecundity and predation incidence.
• In this non‐specialist species, habitat fragmentation, population features and habitat quality affect different facets of plant performance. Predation was the only process clearly affected by fragmentation variables, fecundity mainly depended on population features and offspring performance and was better explained by mother plant identity. Our results show the need to consider habitat and population features together with fragment size and connectivity in order to assess the effects of fragmentation. Importantly, these effects can involve different aspects of plant reproduction, including plant–animal interactions.

     

Nutritional suitability and ecological relevance of <Emphasis Type="Italic">Arabidopsis thaliana</Emphasis> and <Emphasis Type="Italic">Brassica oleracea</Emphasis> as foodplants for the cabbage butterfly, <Emphasis Type="Italic">Pieris rapae</Emphasis>

The thale cress, Arabidopsis thaliana, is considered to be an important model species in studying a suite of evolutionary processes. However, the species has been criticized on the basis of its comparatively small size at maturity (and consequent limitations in the amount of available biomass for herbivores) and on the duration and timing of its life cycle in nature. In the laboratory, we studied interactions between A. thaliana and the cabbage butterfly, Pieris rapae, in order to determine if plants are able to support the complete development of the herbivore. Plants were grown in pots from seedlings in densities of one, two, or four per pot. In each treatment, one, two, or five newly hatched larvae of P. rapae were placed on fully developed rosettes of A. thaliana. In a separate experiment, the same densities of P. rapae larvae were reared from hatching on single mature cabbage (Brassica oleracea) plants. Pupal fresh mass and survival of P. rapae declined with larval density when reared on A. thaliana but not on B. oleracea. However, irrespective of larval density and plant number, some P. rapae were always able to complete development on A. thaliana plants. A comparison of the dry mass of plants in different treatments with controls (= no larvae) revealed that A. thaliana partially compensated for plant damage when larval densities of P. rapae were low. By contrast, single cress plants with 5 larvae generally suffered extensive damage, whereas damage to B. oleracea plants was negligible. Rosettes of plants that were monitored in spring, when A. thaliana naturally grows, were not attacked by any insect herbivores, but there was often extensive damage from pulmonates (slugs and snails). Heavily damaged plants flowered less successfully than lightly damaged plants. Small numbers of generalist plant-parasitic nematodes were also recovered in roots and root soil. By contrast, plants monitored in a sewn summer plot were heavily attacked by insect herbivores, primarily flea beetles (Phyllotreta spp.). These results reveal that, in natural populations of A. thaliana, there is a strong phenological mismatch between the plant and most of its potential specialist insect herbivores (and their natural enemies). However, as the plant is clearly susceptible to attack from non-insect generalist invertebrate herbivores early in the season, these may be much more suitable for studies on direct defense strategies in A. thaliana.     

Evidence that an evolutionary transition from dehiscent to indehiscent fruits in Lepidium (Brassicaceae) was caused by a change in the control of valve margin identity genes

In the Brassicaceae, indehiscent fruits evolved from dehiscent fruits several times independently. Here we use closely related wild species of the genus Lepidium as a model system to analyse the underlying developmental genetic mechanisms in a candidate gene approach. ALCATRAZ (ALC), INDEHISCENT (IND), SHATTERPROOF1 (SHP1) and SHATTERPROOF2 (SHP2) are known fruit developmental genes of Arabidopsis thaliana that are expressed in the fruit valve margin governing dehiscence zone formation. Comparative expression analysis by quantitative RT‐PCR, Northern blot and in situ hybridization show that their orthologues from Lepidium campestre (dehiscent fruits) are similarly expressed at valve margins. In sharp contrast, expression of the respective orthologues is abolished in the corresponding tissue of indehiscent Lepidium appelianum fruits, indicating that changes in the genetic pathway identified in A. thaliana caused the transition from dehiscent to indehiscent fruits in the investigated species. As parallel mutations in different genes are quite unlikely, we conclude that the changes in gene expression patterns are probably caused by changes in upstream regulators of ALC, IND and SHP1/2, possible candidates from A. thaliana being FRUITFULL (FUL), REPLUMLESS (RPL) and APETALA2 (AP2). However, neither expression analyses nor functional tests in transgenic plants provided any evidence that the FUL or RPL orthologues of Lepidium were involved in evolution of fruit indehiscence in Lepidium. In contrast, stronger expression of AP2 in indehiscent compared to dehiscent fruits identifies AP2 as a candidate gene that deserves further investigation.     

Radiocarbon age of carbonate in fruits of Lithospermum from the early Bronze Age settlement of Hirbet ez-Zeraqōn (Jordan)

Uncarbonized fruits of the Boraginaceae occur widely in cultural layers of archaeological sites in the Mediterranean and the Near East. To date, interpreting their origin remains problematic. It is difficult for archaeobotanists to tell whether such fruits were deposited as part of the cultural layer or were introduced post-depositionally. In an attempt to answer this question for the early Bronze Age site of Hirbet ez-Zeraqon (Jordan), we used direct ¹⁴C dating of biogenic carbonate from calcareous fruits of Lithospermum. The radiocarbon ages of seven fossil samples of the fruits suggest that they originate from plants that grew during or around the time of occupation.     

栖息地荒漠化对草原沙蜥食性的影响

栖息地退化会使环境中蜥蜴的可利用食物资源发生改变。采用剖胃法对内蒙古自治区鄂尔多斯市准格尔旗和达拉特旗地区采集的草原沙蜥样本进行了食性分析。对固定沙丘、半固定沙丘和流动沙丘三类样地中77个实胃样本的652只(套)食物进行鉴定和分析的结果表明,这些食物主要为节肢动物,分为35类,隶属3纲11目32科;也在少部分个体(4.69%)的胃容物中发现少量(1.95%)植物碎片。上述三类栖息地中,草原沙蜥对蚁科、蚜科、瓢虫科、叶蝉科和茧蜂科昆虫均有较大的捕食比例;但相比固定沙区栖息地,草原沙蜥在半固定及流动沙丘中增减的食物种类均比较多,导致草原沙蜥在流动沙区与其他两类样地之间有较大的食物相异性。从固定沙丘、半固定沙丘到流动沙丘,随栖息地荒漠化程度的增加,草原沙蜥捕食食物类群的丰富度逐步减少,辛普森优势度指数相应地下降,但营养生态位宽度、香农威纳多样性指数和皮洛均匀性指数却在流动沙丘栖息地上明显增大。这表明鄂尔多斯地区的草原沙蜥是以捕食昆虫等节肢动物为主、兼食极少量植物的杂食性动物。栖息地荒漠化所致的环境变化显著地影响草原沙蜥的食物组成,使其觅食的食物种类逐步减少。     

Compensatory response of two dune annuals to simulated browsing and fruit predation

Abstract. Greenhouse and field studies were conducted to determine the effects of artificial fruit removal and clipping (simulated deer browsing) on components of reproduction of two indeterminate dune annuals: Cakile edentula var. lacustris (Bigel.) Hook and Corispermum hyssopifolium L. The results showed that both species were able to partially or completely compensate or even overcompensate for low to moderate levels of clipping or fruit removal only if the damage occurred in the early stages of fruit development. The indeterminate habit of growth of both species facilitated their compensatory ability. The removal of fruits and clipping of plants which mimick browsing by white tailed deer along Lake Huron resulted in a significant increase in their period of flowering and fruiting, especially in the early stages of fruit development. The plants compensated for fruit loss by increasing the number of fruits per plant. A delay in fruit removal or clipping to later stages of fruit maturation of both species drastically reduced reproduction. Similar experiments in the field revealed that, although the critical periods during which the plants were most susceptible were the same, the plants had lower compensatory ability. Fruit damage and clipping generally did not affect the seed mass of both species.     

Plant genotype and the preference and performance of herbivores: cultivar affects apple resistance to the florivorous weevil Anthonomus pomorum

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Diet of the Critically Endangered Brown Spider Monkey (Ateles hybridus) in an Inter‐Andean Lowland Rainforest in Colombia

Brown spider monkeys (Ateles hybridus) are one of the least known and more threatened primates in the Neotropics. Recognized as a species about a decade ago, field studies on these endangered primates have mainly focused on estimating local population densities. Since 2006, we habituated a group of wild brown spider monkeys at Serranía de Las Quinchas, Colombia, and studied their feeding ecology during 2.5 years using focal “subgroup” sampling, and conducted phenological surveys in order to estimate habitat‐wide fruit availability. Based on 847 hr of behavioral follows, brown spider monkeys spent approximately 25% of their time in feeding activities, and fed from fruits and leaves on at least 123 plant species. Ripe fruits were the most important item in the diet of A. hybridus at Las Quinchas comprising 92% of their feeding time. Probably due to the minor variation in the monthly proportion of fruits in brown spider monkey's diet throughout this study, there was no relation between habitat‐wide fruit availability and the proportion of fruit included in their monthly diet. The diet of brown spider monkeys at Las Quinchas is toward the high end of fruit intake, even within other wild spider monkeys’ populations, suggesting that these endangered primates might also be facing the challenges of being a large bodied fruit specialist under a regional scenario of habitat loss and fragmentation. Am. J. Primatol. 74:1097‐1105, 2012. © 2012 Wiley Periodicals, Inc.     

Effects of genotype,habitat, and seasonal variation on iridoid glycoside content of Plantago lanceolata (Plantaginaceae) and the implications for insect herbivores

Summary We investigated the effects of genotype, habitat, and seasonal variation on production of the iridoid glycosides, aucubin and catalpol, in leaves of the common weed Plantago lanceolata. Two genotypes, one each from a lawn and an adjacent abandoned hayfield population, were clonally replicated in the greenhouse, and then planted back into the two habitats. One quarter of the plants from each treatment were harvested on each of four dates, at approximately two-week intervals. Over the course of the growing season, and in both habitats, we found a significant increase in the concentration of both aucubin and catalpol in P. lanceolata leaves. The genotypes differed in their response to environmental variation, both in time and between sites, as indicated by significant genotype x date and genotype x site interactions. Early in the season, habitat (lawn or field) had a greater effect on iridoid glycoside concentration than did plant genotype, but later in the season, plant genotype was more influential in determining the iridoid glycoside concentration. Thus, the relative palatability of Plantago genotypes to specialist and generalist herbivores may vary in time and space.