共查询到20条相似文献,搜索用时 15 毫秒
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Negative autoregulation of c-myc gene expression is inactivated in transformed cells. 总被引:12,自引:3,他引:9
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F Grignani L Lombardi G Inghirami L Sternas K Cechova R Dalla-Favera 《The EMBO journal》1990,9(12):3913-3922
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TGF-beta regulation of epithelial cell proliferation. 总被引:4,自引:0,他引:4
H L Moses 《Molecular reproduction and development》1992,32(2):179-184
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Y Kubota S H Kim S M Iguchi-Ariga H Ariga 《Biochemical and biophysical research communications》1989,162(3):991-997
In the human neuroblastoma cell line IMR32 the N-myc gene happens to be amplified and actively expressed, whereas no stable c-myc RNA can be detected in the same cells. In this report, we show that in IMR32 cells the expression of the N-myc gene is repressed by introduction of a c-myc expression vector (c-myc cDNA conjugated with an SR promoter). Moreover, dose response experiments showed that the amount of endogenous c-myc protein present in HeLa cells (which express c-myc but not N-myc) is enough to repress the expression of N-myc in IMR32 cells. 相似文献
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Nuclear colocalization of cellular and viral myc proteins with HSP70 in myc-overexpressing cells. 总被引:8,自引:1,他引:7
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The c-myc oncogene and its viral counterpart v-myc encode phosphoproteins which have been located within cell nuclei, excluding nucleoli. We have expressed the c-myc gene under the simian virus 40 early promoter and studied the distribution of its protein product in transient expression assays in COS, HeLa, and 293 cells. We found three distinct patterns of c-myc immunofluorescence in the transfected cells: one-third of the c-myc-positive cells displayed a diffuse nuclear distribution, and in two-thirds of the cells the c-myc fluorescence was accumulated either in small amorphous or in large multilobed phase-dense nuclear structures. Unexpectedly, these structures also stained for the HSP70 heat shock protein in both heat-shocked and untreated cells. Our results indicate that both transient and stable overexpression of either the c-myc or v-myc protein induces translocation of the endogenous HSP70 protein from the cytoplasm to the nucleus, where it becomes sequestered in structures containing the myc protein. Interestingly, the closely related N-myc protein does not stimulate substantial nuclear expression of the HSP70 protein. Studies with chimeric myc proteins revealed that polypeptide sequences encoded by the second exon of c-myc are involved in colocalization with HSP70. 相似文献
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Segaert S Courtois S Garmyn M Degreef H Bouillon R 《Biochemical and biophysical research communications》2000,268(1):237-241
Apigenin, a flavonoid with chemopreventive properties, induces cellular growth arrest, with concomitant inhibition of intracellular signaling cascades and decreased proto-oncogene expression. We report that apigenin potently inhibited vitamin D receptor (VDR) mRNA and protein expression in human keratinocytes without changes in VDR mRNA half-life. Concurrently, downregulation of retinoid X receptor alpha, a dramatic loss of c-myc mRNA, and upregulation of p21(WAF1) took place. Furthermore, a nearly complete suppression of vitamin D responsiveness was observed as estimated by induction of 24-hydroxylase mRNA. The apigenin effect on VDR expression was shared by some other (quercetine and fisetine) but not all tested flavonoids. Interestingly, the apigenin-mediated VDR suppression was counteracted by the NFkappaB inhibitors sodium salicylate and caffeic acid phenethyl ester. The presented results propose suppression of nuclear receptor levels as a novel mechanism whereby flavonoids exert their pleiotropic effects. This study may also contribute to the understanding of the regulation of VDR expression in epidermal keratinocytes. 相似文献
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Identification of c-myc as a down-stream target for pituitary tumor-transforming gene 总被引:43,自引:0,他引:43
Pei L 《The Journal of biological chemistry》2001,276(11):8484-8491