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正交试验设计是天然产物研究中重要的方法之一,本文介绍了利用Microsoft EXCEL的内部统计函数编制天然产物提取工艺统计分析程序,并以实例说明了该程序的使用方法和应用技巧。结果表明,用户只需输入试验的原始数据,即可快速、准确地进行直观分析和方差分析,并可根据极差值得出最佳工艺条件,所得分析结果与专业统计分析软件DPS对数据进行正交分析相比,二者计算结果基本一致,且该方法不但快捷方便,操作简单,而且结果准确可靠,可大大提高天然产物提取工艺的效率。 相似文献
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《生物技术通讯》2016,(6)
目的:试验不同条件,优化Optiprep密度梯度离心方法,建立最佳的Optiprep连续密度梯度,为纯化病毒提供良好的参数。方法:试验不同的旋转角度、重复次数、离心时间,以及用不同浓度的Optiprep制备连续密度梯度等条件,摸索制备连续密度梯度的最佳方案,将此最佳方案应用于纯化丙肝病毒(HCV),并通过q RT-PCR验证纯化效果。结果:通过各种条件的摸索,确立了制备连续密度梯度的最佳方案,即制备10%~40%Optiprep连续密度梯度,在旋转角度为85°时,1号程序设置为30 r/min离心5 s,2号程序设置为0 r/min 15 s,重复1~2程序10次。将此程序用于纯化HCV,得到了较好的纯化效果。结论:建立了制备Optiprep连续密度梯度离心的方法,为进一步提高病毒的纯化效果提供了参数。 相似文献
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土壤铜污染对环境生态的破坏已引起国内外的广泛重视.在制订土壤铜的环境卫生标准时,常涉及土壤有效态铜的参数问题.一般估计土壤有效态铜的方法是通过盆栽试验程序、检测萃取剂从土壤中萃取的铜与植物铜的相关性,但是其结果却因各个研究的试验条件及土样理化性质的不同而有较大差异.在自然条件下,萃取铜是否能有效地反映作物可食部分的铜含量,仍是一个有争议的问题.近年Mathur等根据土壤基本特性对土壤铜有效性的影响,提出了以土壤总铜与土壤阳离子交换量的比值为土壤铜参数,在反映 相似文献
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根据一定试验设计进行田间试验,经过一定观察记载,就可取得一些数据。由于这些数据是对于有限样本的观察得到的,它们通常具有变异性、局部性和偶然性。也就是说,从表面上看这些原始数据是杂乱无章的,必须对它们加以适当整理分析,才能发现其内在的规律,以便能根据试验结果得出科学性结论。数据分组如果数据较为复杂,通常采用分组的方法研究事物的分布及总体结构,同时还能简化运算程序。数据类型不同,分组的方法也不同。易于 相似文献
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目的:采用常用的电子表格处理系统Microsoft Excel解决药学实验过程中遇到的数据分析问题。方法:应用工作表函数中内置的统计函数,以线性回归为例说明源数据的输入与结果返回的具体操作过程;对数据分析工具中的"描述统计"工具、t检验与方差分析,结合具体实例对药学实践中遇到的药学统计实际问题进行综合探讨。结果:用Excel表中内置的统计函数工具进行线性回归分析,方法简单、结果可靠;Excel表中的数据分析工具适用于日常药学实验数据分析过程中遇到的描述统计分析、t检验与方差分析。Excel与其它数据处理软件相比具有操作快捷、使用方便、计算精确、易于学习与掌握等优点。结论:Excel友好的界面,清晰的统计分析结果,使医药工作者在使用Excel的数据分析软件时会感到非常的方便快捷,灵活实用,值得在药学实践中应用推广。 相似文献
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庆大霉素是氨基糖苷类广谱抗生素,其不仅用于临床治病,而且广泛应用于畜牧业。由于其发酵周期长,产素率低,生产成本高,因此改进生产方法势在必行。本文报道了在绛红色小单孢菌产生庆大霉素的培养过程中,添加一定量的甘氨酸、蛋氨酸、赖氨酸、酪氨酸能够有效地提高微生物细胞的代谢能力,缩短发酵培养周期,提高产素率。本方法是在小试(摇瓶)成功基础上,用5L玻璃发酵罐运转一个多月,取一个月的平均罐批数据表明:新方法较原工艺发酵周期缩短30% ~45%,罐批产量增加14% 左右,产素率提高30 % ~95%(因菌种生产能力不同而异),产品质量符合中国药典2000版(CP2000)、英国药典2000版(BP2000)、美国药典26版(USP26),生产成本大幅度降低,具有很强的市场竞争力。 相似文献
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Dr. Kaj Fagervik Mikael Rydström Randolf von Schalien Björn Saxén Knut Ringbom Anette Rothberg Håkan Gros 《Journal of industrial microbiology & biotechnology》1995,14(5):403-411
Summary In order to study and control fermentation processes, indirect on-line measurements and mathematical models can be used. Here an on-line model for fermentation processes is presented. The model is based on atom and partial mass balances as well as on stability equations for the protolytes. The model is given an adaptive form by including transport equations for mass transfer and expressions for the fermentation kinetics. The state of the process can be estimated on-line using the balance component of the model completed with measurement equations for the input and the output flows of the process. Adaptivity is realized by means of on-line estimation of the parameters in the transport and kinetic expressions using recursive regression analysis. On-line estimation of the kinetic and mass transfer parameters makes model-based predictions possible and enables intelligent process control while facilitating testing of the validity of the measurement variables. A practical MS-Windows 3.1 model implementation called FMMS—Fermentation Monitoring and Modeling System is shown. The system makes it easy to configure the operating conditions for a run. It uses Windows dialogs for all set-ups, model configuration parameters, elemental compositions, on-line measurement devices and signal conditioning. Advanced on-line data analysis makes it possible to plot variables against each other for easy comparison. FMMS keeps track of over 100 variables per run. These variables are either measured or estimated by the model. Assay results can also be entered and plotted during fermentation. Thus the model can be verified almost instantly. Historical fermentation runs can be re-analyzed in simulation mode. This makes it possible to examine different signal conditining filters as well as the sensitivity of the model. Combined, the data analysis and the simulation mode make it easy to test and develop model theories and new ideas. 相似文献
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Vanessa Zavatti Hector Budman Raymond Legge Melih Tamer 《Bioprocess and biosystems engineering》2016,39(6):855-869
Fluorescence spectroscopy in combination with multivariate statistical methods was employed as a tool for monitoring the manufacturing process of pertactin (PRN), one of the virulence factors of Bordetella pertussis utilized in whopping cough vaccines. Fluorophores such as amino acids and co-enzymes were detected throughout the process. The fluorescence data collected at different stages of the fermentation and purification process were treated employing principal component analysis (PCA). Through PCA, it was feasible to identify sources of variability in PRN production. Then, partial least square (PLS) was employed to correlate the fluorescence spectra obtained from pure PRN samples and the final protein content measured by a Kjeldahl test from these samples. In view that a statistically significant correlation was found between fluorescence and PRN levels, this approach could be further used as a method to predict the final protein content. 相似文献
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本实验室构建的疟疾DNA疫苗经动物试验表明具有很好的免疫原性,为申请临床试验,进行了制备工艺的研究。本研究将含pcD-awte质粒的大肠杆菌DH5α在发酵罐中发酵培养,碱裂解法粗提质粒,再依次通过Sepharose 6FF分子筛层析、Plasmidselect 亲硫吸附层析和Source 30Q离子交换层析精制获得质粒纯品,并对纯品进行质量分析。结果每升培养液可获得质粒纯品43.9mg,质量符合Ferreira等推荐的药用标准。 相似文献
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Higgins VJ Beckhouse AG Oliver AD Rogers PJ Dawes IW 《Applied and environmental microbiology》2003,69(8):4777-4787
Genome-wide expression analysis of an industrial strain of Saccharomyces cerevisiae during the initial stages of an industrial lager fermentation identified a strong response from genes involved in the biosynthesis of ergosterol and oxidative stress protection. The induction of the ERG genes was confirmed by Northern analysis and was found to be complemented by a rapid accumulation of ergosterol over the initial 6-h fermentation period. From a test of the metabolic activity of deletion mutants in the ergosterol biosynthesis pathway, it was found that ergosterol is an important factor in restoring the fermentative capacity of the cell after storage. Additionally, similar ERG10 and TRR1 gene expression patterns over the initial 24-h fermentation period highlighted a possible interaction between ergosterol biosynthesis and the oxidative stress response. Further analysis showed that erg mutants producing altered sterols were highly sensitive to oxidative stress-generating compounds. Here we show that genome-wide expression analysis can be used in the commercial environment and was successful in identifying environmental conditions that are important in industrial yeast fermentation. 相似文献
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磁敏感氧化亚铁硫杆菌胞内可以生成磁性颗粒,探索其培养条件对趋磁细菌的研究有重要意义。利用补料发酵法,实现了磁敏感氧化亚铁硫杆菌的实验室批量培养。发酵终体积为2L的摇瓶发酵条件下,培养40h后一次补Fe2+(5%),使最高菌体浓度达到2.33×107个/ml,比不补料对照提高了60.69%;同样条件下,补入9K全料则可达到2.47×107个/ml,比不补料对照提高70.34%;原子力显微镜磁扫描结果显示发酵得到的菌体有明显磁性,即胞内含有大量磁性颗粒。 相似文献
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以北里孢菌(Kitasatospora sp.)MY 5-36为供试菌株,对ε-聚赖氨酸分批补料发酵动力学模型进行研究。建立了该菌株发酵合成ε-聚赖氨酸的菌体生长、产物合成和总糖消耗的动力学模型,并通过Origin 8.1软件对模型参数进行非线性拟合。结果表明:菌体量和聚赖氨酸的产量分别为16.25和13.15 g/L,产物合成与菌体生长的关系为部分耦联型。经验证,预测值与实验值有良好的拟合性,拟合度分别为0.999、0.995和0.992,说明所构建模型能够较好地反映ε-聚赖氨酸分批补料发酵过程。 相似文献