10种晋产野生黄芩根中黄芩苷、黄芩素与汉黄芩素含量比较

采用高效液相色谱法对山西省10个产地野生黄芩根中主要有效成分黄芩苷、黄芩素和汉黄芩素含量进行了测定分析。结果表明,10种晋产野生黄芩根中主要有效成分含量差异明显,黄芩苷的含量为5.79%~15.31%,黄芩素的含量为0.49%~2.28%,汉黄芩素的含量为0.07%~0.42%。黄芩苷含量与纬度高低呈负相关。山西北部朔州市右玉县沟北村与山阴县西郭家窑村的野生黄芩根中黄芩苷含量低于《中国药典》规定的8.0%,山西东南部晋城市陵川县瓦窑上村的野生黄芩根中黄芩苷含量则高达15.31%。山西中西部吕梁市交城县双家寨村的野生黄芩根中黄芩素和汉黄芩素含量均为最高,分别为2.28%和0.42%。     

HPLC同时测定龙胆泻肝制剂中黄芩苷、汉黄芩苷、黄芩素、汉黄芩素的含量

目的:建立同时测定龙胆泻肝制剂中黄芩苷、汉黄芩苷、黄芩素和汉黄芩素4种有效成分含量的HPLC分析方法.方法:色谱柱:Dikma Diamonsil C18柱(250 mm×4.6 mm,5μm);流动相:乙腈- 0.05％磷酸溶液,梯度洗脱;波长:278 nm.结果:黄芩苷、汉黄芩苷、黄芩素和汉黄芩素的线性范围分别为0.418～4.18μg(r=0.9999),0.2～2μg(r=0.9999),0.233～2.33 μg(r=0.9999),0.215～2.15(r=0.9998).2种制剂4种成分的平均回收率分别为98.10％ (RSD=0.59％),98.05％( RSD=1.37％),98.88％( RSD=1.44％),98.10％( RSD =0.59％)和98.40％ (RSD=0.46％),99.33％ (RSD=1.74％),99.09％( RSD=1.22％),98.82％(RSD=1.42％).结论:该方法快速、简便、准确、具有良好的重复性和回收率,可作为龙胆泻肝制剂中这4种成分的含量测定方法.     

黄芩产量和黄芩苷含量对氮磷钾肥料的响应

通过田间试验,采用氮、磷、钾三因素二次饱和D-最优设计施肥方案,于收获期计算黄芩产量和根中黄芩苷含量,建立以氮、磷、钾施用量为自变量,黄芩产量和根中黄芩苷含量为目标函数的三元二次多项式数学模型,通过对模型进行解析、寻优获得最适施肥量.结果表明:氮、磷对黄芩产量有显著影响,在低水平施肥条件下,产量随施肥量的增加而增大,一定范围后,增效不明显;氮、磷、钾对黄芩苷含量均有显著影响,黄芩苷含量随氮、钾施用量增加呈先降低再增高的趋势,在低水平施磷条件下,黄芩苷含量随磷肥施用量增加而增加,最终趋于稳定.氮磷、氮钾、磷钾对黄芩产量和黄芩苷含量存在明显的交互作用.本试验条件下,黄芩产量超过4000 kg·hm-2、根中黄芩苷含量14％以上的施肥方案为氮(N)90.5～104.7 kg· hm-2、磷(P2O5) 163.9 ～ 199.9 kg·hm-2、钾(K2O)84.1 ～140.8 kg·hm-2,比例约为1∶1.86∶1.15.     

安宫牛黄丸中黄芩苷和黄芩素的含量测定

目的:利用高效液相色谱法建立安宫牛黄丸中黄芩苷和黄芩素含量的测定方法.方法:采用高效液相色谱法.以VenusilASB-C18柱(4.6mm×250mm,5μm)为色谱柱;流动相分别为:乙腈-0.4%磷酸(21:79;35:65);检测波长:277 nm;流速:1 ml.min-1.结果:安宫牛黄丸中黄芩苷和黄芩素的含量分别为0.148%;0.0169%.结论:该方法准确、灵敏度高、重现性好,可以作为安宫牛黄丸中黄芩苷和黄芩素含量的测定方法.     

UV和HPLC法测定黄芩黄酮总苷元提取物中总黄酮和3种主要成分的含量

本文建立了黄芩黄酮总苷元提取物中总黄酮和3种主要成分的含量测定方法。采用HPLC法测定黄芩素、汉黄芩素和千层纸素A的含量。使用Agilent 1200型高效液相色谱仪,Eclipse XDB-C18(250 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-0.2%磷酸水(50∶50),流速1 m L/min,柱温35℃,检测波长275 nm的方法。黄芩素、汉黄芩素和千层纸素A线性范围分别为3.501~70.016μg/m L(r=0.9999),0.832~16.640μg/m L(r=0.9998),0.418~8.352μg/m L(r=0.9999);平均加样回收率(n=6)分别为99.55%、98.86%、9.997%,RSD分别为1.74%、1.74%、1.70%。采用UV法测定总黄酮含量,黄芩素线性范围为1.71~8.54μg/m L(r=0.9991);平均加样回收率(n=6)为100.93%,RSD为2.63%。黄芩素、汉黄芩素、千层纸素A和总黄酮的含量分别为48.22%~60.36%、12.09%~14.46%、5.05%~8.81%、78.11%~90.12%;研究结果表明该方法准确、可靠,适用于黄芩黄酮总苷元提取物的含量测定。     

一株发酵转化黄芩苷菌株的筛选及鉴定

为实现生物发酵法转化黄芩苷生产活性产物黄芩素,本研究从自然发毛的新鲜黄芩植株根部筛选发酵转化黄芩苷生成黄芩素的菌株,并进行产物分析和菌株鉴定。通过马铃薯葡萄糖培养液(PDB)富集,黄芩药粉培养液初筛,马铃薯葡萄糖琼脂(PDA)平板分离后转入PDB黄芩苷培养液复筛,高效液相色谱法定性及定量分析,得到一株能够高效转化黄芩苷为黄芩素的菌株RM3,该菌在添加0. 1%黄芩苷的PDB培养液中能够将黄芩苷转化为黄芩素,未经任何优化条件下,28℃,150 rpm培养5天后摩尔转化率达到83. 87%。通过对菌落的形态、显微结构观察及ITS序列分析比对,鉴定菌株RM3为青霉菌(Penicillium sp. RM3)。该菌转化黄芩苷生成黄芩素效率高,且清洁环保,可缓解中药资源紧张,满足市场和临床需求,是一株很有前途的黄芩素生产菌。     

离子液体-微波辅助提取黄芩饮片中四种黄酮类成分(英文)

采用HPLC法建立黄芩饮片中黄芩苷、汉黄芩苷、黄芩素、汉黄芩素四种黄酮类化合物同时测定的方法;并且优选离子液体-微波辅助提取(IL-MAE)黄芩饮片中四种黄酮类成分的最佳工艺。优选的色谱分离条件为:Aglient TC-C18(250×4.6 mm,5.0μm)色谱柱,流动相为0.4%磷酸水(A)和甲醇(B),梯度洗脱程序为0~10min,40%~50%B;10~20 min,50%B;20~30 min,50%~60%B;30~40 min,60%~80%B;50 min,80%B,检测波长280 nm。优选的最佳的黄芩饮片IL-MAE条件为:以溴化-1-丁基-3-甲基咪唑盐为溶剂,固-液比为1∶100,提取时间为3 min,微波功率为264 W;将该工艺所得四种成分的含量与采用中国药典(2010版)所收载的"回流提取法"所得含量进行对比,IL-MAE显著地提高了黄芩饮片中四种黄酮类成分的含量并且缩短了提取时间。研究结果表明,所建立的HPLC分析方法灵敏、准确,可用于黄芩饮片的质量控制;所优选的黄芩饮片的IL-MAE简单,高效,快速,无污染。     

海南黄芩中汉黄芩素抗肿瘤活性研究

目的:分离纯化海南黄芩中黄酮类化合物汉黄芩素,并研究其抗肿瘤活性。方法:经乙醇回流提取,石油醚和氯仿萃取,采用半制备液相色谱技术对海南黄芩中的汉黄芩素进行分离纯化,NMR等技术进行结构鉴定;采用常见肿瘤肺癌、肝癌、宫颈癌细胞,利用MTT法测定汉黄芩素的细胞毒活性;采用S180移植荷瘤小鼠,对汉黄芩素进行体内肿瘤实验,随机分成4组,阴性对照组给予生理盐水,阳性对照组尾静脉注射10 mg/kg市售紫杉醇注射液,药物低、高剂量组分别灌胃给予10、40 mg/kg海南汉黄芩素,连续7 d;最终应用荷瘤动物模型评价汉黄芩素的抗肿瘤功效。结果:从海南黄芩中分离得到汉黄芩素,并通过NMR波谱进行结构鉴定予以确认;细胞毒活性评估结果显示汉黄芩素对Hep G2、He La和A549细胞均显示显著的抑制活性;体内抗肿瘤试验显示汉黄芩素能明显抑制肿瘤体积和重量,抗肿瘤作用显著,并呈现一定的量效关系。结论:黄酮类化合物汉黄芩素为首次从海南黄芩中分离得到,抗肿瘤活性显示其有很强的抗肿瘤活性,这为深入研究与开发海南黄芩提供科学依据。     

离子液体-微波辅助提取黄芩饮片中四种黄酮类成分(英文)北大核心CSCD

采用HPLC法建立黄芩饮片中黄芩苷、汉黄芩苷、黄芩素、汉黄芩素四种黄酮类化合物同时测定的方法;并且优选离子液体-微波辅助提取（IL-MAE）黄芩饮片中四种黄酮类成分的最佳工艺。优选的色谱分离条件为：Aglient TC-C18（250×4.6 mm,5.0μm）色谱柱,流动相为0.4%磷酸水（A）和甲醇（B）,梯度洗脱程序为0-10min,40%-50%B;10-20 min,50%B;20-30 min,50%-60%B;30-40 min,60%-80%B;50 min,80%B,检测波长280 nm。优选的最佳的黄芩饮片IL-MAE条件为：以溴化-1-丁基-3-甲基咪唑盐为溶剂,固-液比为1∶100,提取时间为3 min,微波功率为264 W;将该工艺所得四种成分的含量与采用中国药典（2010版）所收载的＂回流提取法＂所得含量进行对比,IL-MAE显著地提高了黄芩饮片中四种黄酮类成分的含量并且缩短了提取时间。研究结果表明,所建立的HPLC分析方法灵敏、准确,可用于黄芩饮片的质量控制;所优选的黄芩饮片的IL-MAE简单,高效,快速,无污染。     

UV和HPLC法测定黄芩黄酮总苷元提取物中总黄酮和3种主要成分的含量北大核心CSCD

本文建立了黄芩黄酮总苷元提取物中总黄酮和3种主要成分的含量测定方法。采用HPLC法测定黄芩素、汉黄芩素和千层纸素A的含量。使用Agilent 1200型高效液相色谱仪,Eclipse XDB-C18（250 mm×4.6 mm,5 μm）色谱柱,流动相为甲醇-0.2%磷酸水（50：50）,流速1 mL / min,柱温35 ℃,检测波长275 nm的方法。黄芩素、汉黄芩素和千层纸素 A线性范围分别为3.501-70.016 μg/mL（r=0.9999）,0.832-16.640 μg/mL（r=0.9998）,0.418-8.352 μg/mL（r=0.9999）;平均加样回收率（n=6）分别为99.55%、98.86%、9.997%,RSD分别为1.74%、1.74%、1.70%。采用UV法测定总黄酮含量,黄芩素线性范围为1.71-8.54 μg/mL（r=0.9991）;平均加样回收率（n=6）为100.93%,RSD为2.63%。黄芩素、汉黄芩素、千层纸素 A和总黄酮的含量分别为48.22%-60.36%、12.09%-14.46%、5.05%-8.81%、78.11%-90.12%;研究结果表明该方法准确、可靠,适用于黄芩黄酮总苷元提取物的含量测定。     

Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi

Free radical scavenging and antioxidant activities of baicalein, baicalin, wogonin and wogonoside, the four major flavonoids in the radix of Scutellaria baicalensis Georgi, were examined in different systems. ESR results showed that baicalein and baicalin scavenged hydroxyl radical, DPPH radical and alkyl radical in a dose-dependent manner, while wogonin and wogonoside showed subtle or no effect on these radicals. Ten micromol/l of baicalein and baicalin effectively inhibited lipid peroxidation of rat brain cortex mitochondria induced by Fe(2+)-ascorbic acid, AAPH or NADPH, while wogonin and wogonoside showed significant effects only on NADPH-induced lipid peroxidation. In a study on cultured human neuroblastoma SH-SY5Y cells system, it was found that 10 micromol/l of baicalein and baicalin significantly protected cells against H(2)O(2)-induced injury. Baicalein was the most effective antioxidant among the four tested compounds in every system due to its o-tri-hydroxyl structure in the A ring. Compared with a well-known flavonoid, quercetin, the antioxidant activity of baicalein was lower in DPPH or AAPH system, but a little higher in those systems which might associate with iron ion. These results suggest that flavonoids in the radix of Scutellaria baicalensis with o-di-hydroxyl group in A the ring, such as baicalein and baicalin, could be good free radical scavengers and might be used to cure head injury associated with free radical assault.     

Deep Sequencing of the Scutellaria baicalensis Georgi Transcriptome Reveals Flavonoid Biosynthetic Profiling and Organ-Specific Gene Expression

Scutellaria baicalensis Georgi has long been used in traditional medicine to treat various such widely varying diseases and has been listed in the Chinese Pharmacopeia, the Japanese Pharmacopeia, the Korean Pharmacopoeia and the European Pharmacopoeia. Flavonoids, especially wogonin, wogonoside, baicalin, and baicalein, are its main functional ingredients with various pharmacological activities. Although pharmaological studies for these flavonoid components have been well conducted, the molecular mechanism of their biosynthesis remains unclear in S. baicalensis. In this study, Illumina/Solexa deep sequencing generated more than 91 million paired-end reads and 49,507 unigenes from S. baicalensis roots, stems, leaves and flowers. More than 70% unigenes were annotated in at least one of the five public databases and 13,627 unigenes were assigned to 3,810 KEGG genes involved in 579 different pathways. 54 unigenes that encode 12 key enzymes involved in the pathway of flavonoid biosynthesis were discovered. One baicalinase and three baicalein 7-O-glucuronosyltransferases genes potentially involved in the transformation between baicalin/wogonoside and baicalein/wogonin were identified. Four candidate 6-hydroxylase genes for the formation of baicalin/baicalein and one candidate 8-O-methyltransferase gene for the biosynthesis of wogonoside/wogonin were also recognized. Our results further support the conclusion that, in S. baicalensis, 3,5,7-trihydroxyflavone was the precursor of the four above compounds. Then, the differential expression models and simple sequence repeats associated with these genes were carefully analyzed. All of these results not only enrich the gene resource but also benefit research into the molecular genetics and functional genomics in S. baicalensis.     

小胶质细胞在脂多糖引起的热高敏中的作用

目的探讨小胶质细胞在脂多糖引起的热高敏中的作用。方法清洁级雄性昆明小鼠,随机分成两组,每组5只,腹腔注射LPS组和注射PBS组,在注射前及后30、60、120、240 min测量小鼠足底的热痛阈;每组于注射前及后4h各处死5只取脑组织检测IL-1β、TNF-α;每组于腹腔注射4h时处死动物,免疫荧光确定脑组织中小胶质细胞的激活情况。然后分为四组,米诺环素+PBS组,米诺环素+LPS组,PBS+PBS组,PBS+LPS组,每组5只,连续三天腹腔注射米诺环素或PBS,第三天注射LPS或PBS,在注射前及后30、60、120、240 min测量小鼠足底的热痛阈;每组于注射前及后4h各处死5只取脑组织检测IL-1β、TNF-α。结果与注射PBS相比,注射LPS导致IL-1β、TNF-α分泌增加,注射60、120、240 min小鼠的热痛阈降低;与米诺环素+PBS组、米诺环素+LPS组、PBS+PBS组相比,PBS+LPS组导致IL-1β、TNF-α分泌增加,注射60、120、240 min小鼠的热痛阈降低。结论LPS激活小胶质细胞分泌促炎细胞因子导致热高敏。     

胃饥饿素对小鼠急性肺损伤的保护作用及其机制研究

目的 探讨胃饥饿素对小鼠急性肺损伤的保护作用和机制.方法 将60只小鼠采用随机数字表法分为6组:对照组、模型组、胃饥饿素低、中、高剂量组和地塞米松组.对照组和模型组腹腔注射0.2 mL生理盐水,胃饥饿素各组分别注射400、200、100 μg/kg溶液,地塞米松组注射2 mg/kg.给药后1h,对照组滴注等体积生理盐水...     

低氧联合LPS刺激对星形胶质细胞中炎性因子和BNIP3表达的影响*

目的:探讨在低氧联合脂多糖(LPS)作用下,星形胶质细胞中B淋巴细胞瘤-2/腺病毒E1B 19-kD相互作用蛋白3(BNIP3)的表达和炎症反应变化。方法:将体外培养的原代星形胶质细胞和神经元进行下列分组:常氧组、LPS组、低氧组和LPS+低氧组(每组设置3个复孔)。LPS处理后,低氧组和LPS+低氧组放入低氧细胞孵箱,LPS组和常氧组放入正常的细胞孵箱。LPS浓度:100 ng/ml,氧气浓度为0.3%。处理时间为24 h。原代的星形胶质细胞进行上述的分组,时间点设为6 h、12 h和24 h。Western blot检测BNIP3的表达变化,RT-PCR和ELISA分别检测星形胶质细胞的肿瘤坏死因子-ɑ(TNF-ɑ)、白细胞介素-1β(IL-1β)和白细胞介素6(IL-6)mRNA水平变化和分泌情况。结果:与常氧组比较,低氧组炎症因子的表达没有变化,LPS组和LPS＋低氧组的炎症因子TNF-ɑ、IL-1β和IL-6 mRNA水平升高(P＜0.01);与LPS组比较,LPS＋低氧组炎症因子IL-1β和IL-6 mRNA水平进一步升高(P＜0.05,P＜0.01)。与常氧组比较,低氧组炎症因子的分泌水平没有变化,LPS组和LPS＋低氧组的炎症因子TNF-ɑ和IL-6 分泌水平升高(P＜0.01),IL-1β的水平没有变化;与LPS组比较,LPS＋低氧组炎症因子TNF-ɑ和IL-6分泌水平没有进一步升高。BNIP3在体外培养的神经元和星型胶质细胞中都有表达;在星形胶质细胞中,与常氧组比较,LPS组BNIP3的表达没有变化,低氧组和LPS+低氧组BNIP3的表达明显增加(P＜0.01);在神经元中,与常氧组比较,LPS组BNIP3的表达没有变化,低氧组和LPS+低氧组BNIP3的表达增加(P＜0.05,P＜0.01);与神经元的低氧组比较,星形胶质细胞的低氧组BNIP3的表达增加更明显(P＜0.01)。在星形胶质细胞中LPS联合低氧刺激6、12、24 h后BNIP3蛋白的表达,与常氧组相同时间点比较,LPS组BNIP3的表达没有变化,低氧组和LPS+低氧组BNIP3的表达增加(P＜0.05,P＜0.01);与低氧组相同时间点比较,6 h和12 h的LPS＋低氧组BNIP3的表达增加的更高(P＜0.01)。结论:低氧联合LPS刺激可以增强星形胶质细胞的炎症反应,LPS能增加低氧下星形胶质细胞中BNIP3的表达,提示BNIP3在星形胶质细胞的炎性反应中可能具有一定的调节作用。     

小鼠骨髓源性树突状细胞体外诱导培养方法的比较研究

目的探讨最佳体外诱导培养小鼠成熟树突状细胞（dendritic cells,DC）的方法。方法分离、纯化6周龄C57BL／6小鼠骨髓单核细胞,以含10％胎牛血清、20ng／ml重组小鼠粒细胞-巨噬细胞集落刺激因子（GM—CSF）和10ng／ml重组小鼠白细胞介素-4（IL-4）的RPMI-1640培养基培养7d,然后将细胞分成对照未刺激组、肿瘤坏死因子-α（TNF-α）刺激组和TNF-α＋脂多糖（lipopolysaccharides,LPS）刺激组。继续培养48h后,观察各组细胞形态,检测IL-12、IL-6浓度及细胞表面标志CD11c、CD80、CD86和MHC II。结果培养9d后,两刺激组培养的细胞经相差显微镜观察有DC生长。TNF—α刺激组细胞培养上清液中IL-6、IL-12含量显著高于对照组（P〈0．01）,但显著低于TNF—α＋LPS刺激组（P〈0．05）。3组均高表达CD11c,各组间无显著差异;而CD80、CD86和MHC II表达阳性率TNF-α刺激组显著高于对照组（P〈0．01）,TNF-α＋LPS刺激组显著高于单纯TNF—α刺激组（P〈0．05）。结论联合使用TNF-α与LPS刺激可使DC成熟度提高,分泌IL-6、IL-12增加。     

Efficacy of wogonin in the production of immunoglobulins and cytokines by mesenteric lymph node lymphocytes in mouse colitis induced with dextran sulfate sodium

We previously examined wogonin, isolated from Scutellaria baicalensis, chemical mediators, and IgE by mesenteric lymph node (MLN) lymphocytes in rats. The present study explores the effect of wogonin on the MLN lymphocyte function of mice given orally at 20 mg/kg for 2 weeks with dextran sulfate sodium (DS)-induced colitis. The results indicate that IgA levels in MLN lymphocytes were high, while IgE was low, in mice given wogonin compared to those given water. Also, fecal IgA concentration of DS in the wogonin group mice was significantly higher than in the DS group. Concentrations of interferon-gamma and interleukin (IL)-2 of T cells by concanavalin A treatment was significantly higher in the wogonin fed group than in the normal group. Activation-induced IL-4, IL-5 and IL-10 secretion was lower in wogonin fed mice compared control mice after DS-induced colitis. For these reasons, we conclude that wogonin can alleviate the inflammation in DS-induced colitis brought about by an abnormal Th(2) response.     

L-Arg对LPS诱导的急性肺损伤大鼠肺表面活性物质和肺泡巨噬细胞功能的影响

目的:探讨L-精氨酸(L-Arg)对脂多糖(LPS)诱导的急性肺损伤大鼠肺表面活性物质和肺泡巨噬细胞功能的影响。方法:舌下静脉注射脂多糖(LPS)复制肺损伤模型。健康雄性SD大鼠48只,随机分为对照组、模型组(LPS组)和L-Arg治疗组(L-Arg组)(n=16)。分别于给予LPS 3 h或6 h后给予生理盐水(对照组及LPS组,ip)和L-Arg(500 mg/kg ip)(L-Arg治疗组),治疗3 h。原位杂交法(ISH)检测肺组织中肺表面活性蛋白A(SP-A)mRNA的表达;测定肺泡灌洗液(BALF)中的总蛋白(TP)。体外分离培养大鼠肺泡巨噬细胞,以LPS(终浓度10 mg/L)处理巨噬细胞,观察L-Arg对肺泡巨噬细胞的影响。结果:与对照组比较,大鼠肺损伤后SP-A mRNA表达减弱,BALF中TP增多(P<0.01)。肺损伤3 h用L-Arg治疗3 h后,SP-A mRNA阳性细胞表达明显增强,BALF中TP较LPS组相同时间点明显降低(P<0.05,P<0.01),肺损伤减轻。体外实验中,与正常对照组相比,LPS组细胞培养上清中乳酸脱氢酶(LDH)、一氧化氮(NO)、肿瘤坏死因子-α(TNFα-)和白细胞介素-6(IL-6)浓度明显增高(P<0.01);L-Arg明显减少LPS所致的LDH的释放,降低TNFα-和IL-6浓度。结论:L-Arg可减轻内毒素性肺损伤,此机制可能与增强SP-AmRNA表达有关;LPS可刺激巨噬细胞分泌促炎因子和NO,L-Arg可抑制LPS对巨噬细胞的作用。     

Sirtuin 6 overexpression relieves sepsis-induced acute kidney injury by promoting autophagy

Sirtuin 6 (SIRT6) has the function of regulating autophagy. The aim of this study was to investigate the mechanism through which SIRT6 relieved acute kidney injury (AKI) caused by sepsis. The AKI model was established with lipopolysaccharides (LPS) using mice. Hematoxylin-eosin (HE) staining and streptavidin-perosidase (SP) staining was used to observe kidney tissue and test SIRT6 and LC3B proteins in kidney. Enzyme-linked immunosorbent assay (ELISA) was performed to detected the tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) concentrations. Cell counting kit-8 (CCK-8) assay and flow cytometry were carried out to test the cell viability and apoptosis rate respectively. Protein and mRNA were determined by Western blot and quantitative real-time polymerase chain reaction (qRT-PCR). AKI induced by LPS had self-repairing ability. At 12 h after modeling, the expression levels of TNF-α, IL-6, SIRT6 and LC3B-II/LC3B-I were first significantly increased and were then significantly decreased at 48 h after modeling. LPS inhibited the growth of HK-2 cells and promoted the expressions of TNF-α, IL-6, SIRT6 and LC3B. Overexpression of SIRT6 down-regulated the secretion of TNF-α and IL-6 induced by LPS. SIRT6 overexpression inhibited apoptosis induced by LPS and promoted autophagy in HK-2 cells. Silencing of the SIRT6 gene not only promoted the secretion of TNF-α and IL-6 by HK-2 cells, but also promoted apoptosis and reduced autophagy. LPS up-regulated the expression of SIRT6 gene in HK-2 cells. Overexpression of the SIRT6 gene could inhibit apoptosis and induce autophagy, which might be involved in repairing kidney damage caused by LPS.     

Analysis of Influence of Baicalin Joint Resveratrol Retention Enema on the TNF-α, SIgA,IL-2, IFN-γ of Rats with Respiratory Syncytial Virus Infection

Explore the influence of baicalin joint resveratrol retention enema on TNF-α, SIgA, IL-2, and IFN-γ of rats with respiratory syncytial virus (RSV) infection. The 60 SD rats were randomly divided into normal group, model group, baicalin group, resveratrol group, joint group, and ribavirin group. For model group, baicalin group, resveratrol group, joint group, and ribavirin group, rats were given RSV virus suspension intranasally for 3 days, and model group was not given administration. Baicalin group, resveratrol group, joint group, and ribavirin group were, respectively, given baicalin 100 mg/kg/day, resveratrol 30 mg/kg/day, baicalin joint resveratrol, and ribavirin 1 g/kg/day retention enema. After continuously given administration 7 days, rats were measured in serum TNF-α, IL-2, IFN-γ levels and SIgA levels in bronchoalveolar lavage fluid. Model group, TNF-α, IL-2, IFN-γ, and SIgA were significantly higher than the normal group (P < 0.05); Baicalin group, resveratrol group, ribavirin group, TNF-α, IL-2, IFN-γ, and SIgA were significantly higher than the model group (P < 0.05); TNF-α, IL-2 between baicalin group, resveratrol group, ribavirin group, have no significant difference (P > 0.05); Baicalin group, resveratrol group, joint group, IFN-γ, and SIgA were significantly higher than the ribavirin group (P < 0.05); Joint group TNF-α, IL-2, IFN-γ, and SIgA were significantly higher than baicalin group, resveratrol group, and ribavirin group (P < 0.05). Baicalin joint resveratrol retention enema can increase RSV infection model in rats serum TNF-α, IL-2, IFN-γ levels and SIgA levels in bronchoalveolar lavage fluid, which may anti-virus through this mechanism.