爱氏苏木精整体染色及番红—固绿双重染色滴染法在石蜡切片中的运用

我校使用爱氏苏木精整体染色制作根尖、茎尖、花芽分化及胚胎学等的石蜡切片,使用番红-固绿滴染法制作根、茎、叶等营养器官及花药、子房等生殖器官的石蜡切片,已有二十年左右的历史,广泛应用于教学及科研中。由于方法简易、节约时间、用具、     

爱氏苏木精整体染色制片复染法的一些改进

爱氏苏木精整体染色制片复染法的一些改进采用爱氏苏木精（Ｅｈｒｌｉｃｈ）对植物花药、子房、根尖等材料进行整体染色制片,其最大优点在于材料先染色,后包埋,切片后只需脱蜡即可封片。这样,可在较短时间内获得效果较好、数量较多的切片。但这种制片方法在有些植物中...     

快速石蜡切片法在免疫组化染色中的应用

免疫组化染色在病理诊断中发挥着重要的作用,较多的应用于肿瘤的诊断和鉴别诊断,而制作出良好的组织切片是免疫组化染色的基础和前提。快速石蜡制片是病理检验的常规技术之一,我们将常     

爱氏苏木精整体染色法在树脂薄切片中的应用

在组织和细胞学研究中,整体染色法是一项很有用的技术。树脂薄切片技术是近20年来发展起来的一门新技术,它通常是先切后染,这样前后几次染色很难均一。对于需要长时间染色的材料就更显麻烦。另外,常用于薄切片中的染色剂甲苯胺蓝—O、亚甲基蓝和大青Ⅱ在用树胶封片制成永久切片后,颜色会逐渐退去,不能长期保存。为了解决这些问题,我们在掌叶大黄的胚胎学研究中,以掌叶大黄(Rheum palmatum)为材料,     

超声波在植物切片整体染色中的应用

一、引言动、植物切片整体染色法,是从事切片工作者长期待解决,并为许多从事切片的技术人员尝试的一个方法。但在植物切片两色整染中,效果往往不能令人满意。这个方法因为操作简单,时间经济,可节省人力、物力、     

人体及动物组织H.E染色石蜡切片法的技术改进

报道人体及动物组织石蜡切片方法的全面革新技术。以组织块的固定、苏木精染色和脱水同时进行为主要技术特征,改进了某些操作细节,获得了良好的效果。     

金胺O荧光染色在石蜡组织切片麻风病诊断中的应用

目的为了验证金胺O荧光染色法应用于石蜡组织切片麻风杆菌检测的可行性。方法用金胺O荧光法对6例确诊为麻风病的病理组织切片进行染色,并与抗酸染色结果进行对比。结果荧光染色法6例结果均为阳性,在暗背景下麻风杆菌显示明亮淡绿色荧光;在菌量较少荧光染色片中寻找单根麻风杆菌,较抗酸染色片更为容易。结论金胺O荧光染色法可用于石蜡组织切片麻风病的诊断,麻风杆菌单根散在时比抗酸染色法有一定优势。     

HistoGel预包埋法在微小组织石蜡切片中的应用

目的：探讨微小组织HistoGel预包埋的石蜡切片制作方法和优势。方法：选择拟胚体(embryoid body, EB)为微小组织,将人 类胚胎干细胞(human Embryonic Stem Cell, hESC)切割成小块,用拟胚体培养基在低贴附培养皿中悬浮培养形成拟胚体。收集拟 胚体,4 %的多聚甲醛固定。将HistoGel 加热到60 ℃熔解,离心去除拟胚体中的固定液,把液态的HistoGel 加到拟胚体上,调整拟 胚体的相对位置使其相对集中,待胶冷却凝固,将含有拟胚体的胶块取出,进行常规的石蜡切片操作,包括脱水、透明、浸蜡、包 埋、切片和苏木素- 伊红(hematoxylin-eosin, HE)染色。显微镜下观察拟胚体的形态,并用拟胚体的形态完好度来评判这种方法。结 果：HistoGel 仅在60 ℃便可熔解,室温可以冷却凝固,含有拟胚体的胶块在整个操作过程中很方便。展片过程中,石蜡和HistoGel 能够保持平整。HE 染色的结果可以看出,拟胚体内部结构完好,细胞核和细胞质清晰可辨。结论：HistoGel可以作为一种微量细 胞组织的预包埋胶制作石蜡切片,而且相比琼脂预包埋,HistoGel 因其操作更加方便和特有的物理特性显示出更多的优点。     

双氧水快速氧化法在Mallory磷钨酸-苏木素染色中的应用

在日常病理诊断、鉴别诊断及科研工作中,观察横纹肌的基本病理变化,以及对横纹肌肉瘤与许多未分化的间叶性肿瘤的鉴别诊断,多采用Mal-lory磷钨酸—苏木素染色法。但在应用过程中,我们发现该法染色时间长(12—48h),所配染液成熟周期长(数周-数月),从而使临床病理诊断和鉴别诊断受到很大影响。我们根据染色的基本原理,经反复实验,对染色步骤进行了改进;同时采用双氧水快速氧化法,加速染液成熟,收到了理想的染色效果。材料和方法1.材料本科活检及尸检的正常肌肉及横纹肌肿瘤组织,经Zenker氏液固定,常规石蜡切片,切片厚4μm。2.仪器YWY781B医…     

Novel Fixation of Plant Tissue, Staining through Paraffin with Alcian Blue and Hematoxylin, and Improved Slide Preparation

Onion (Allium cepa) root tips were fixed in a proprietary solution without aldehyde, toxic metals or acetic acid. Fixed specimens were embedded in paraffin, sectioned on a rotary microtome and mounted on detergent-washed slides without adhesive. Slides with ribbon segments affixed were immersed in 0.2% aqueous alcian blue 8GX in screw-capped Coplin jars in a water bath at 50 C for 1 hr. Excess alcian blue was rinsed off under cold running tap water and the slides were immersed in quick-mixed hematoxylin at room temperature for 15 min. Stained slides were deparaffinized, rinsed with isopropanol, air dried, and coverslips were affixed with resin. Thus, the traditional paraffin microtechnique has been modified at all steps from fixation to finishing slides with coverslips.     

Block Staining of Mammalian Tissues with Hematoxylin and Eosin

Various mammalian tissues were stained en bloc with hematoxylin and eosin after fixation and prior to embedding in paraffin wax and sectioning. The choice of fixative is important and best results are obtained using Worcester's Fluid, a combination of saturated aqueous mercuric chloride, formaldehyde, and glacial acetic acid. After fixation, blocks of tissue up to 1.5 cm thick are stained for seven days in hematoxylin. Excess stain is removed by washing tissues in running water overnight. Tissue blocks then are dehydrated with graded concentrations of ethyl alcohols to 80% and counterstained, with further dehydration, in 0.5% spirit soluble eosin in 90% ethyl alcohol for five days. The tissue is subsequently transferred to 90% ethyl alcohol overnight to differentiate eosin staining; dehydration is completed in absolute ethyl alcohol. The blocks are cleared in cedarwood oil and briefly in xylene prior to embedding, sectioning, and mounting. Following removal of wax by xylene, coverslips are applied.

General morphological and histological features were particularly well differentiated and very selectively and reliably stained by this method.     

苏木精染色液的改进和使用

报道了一种新的特制苏木精染色液的配制、使用方法、染色效果及其使用价值。实验表明,该染色液全面而明显地优于通用的Harris染色液。     

The Hortega Silver Carbonate Method for Staining Pituicytes After Paraffin Embedding

In three papers published on pituicytes, of the ox by Bucy (1930), of the human by Shanklin (1940), and of the horse by Vazquez-Lopez (1942) the pituitaries were sectioned by the freezing method and stained by the Hortega silver carbonate technic. Since that time, as a routine procedure in our laboratory, frozen sections have been replaced by paraffin which in no way interferes with the Hortega silver carbonate staining.     

The Hortega Silver Carbonate Method for Staining Pituicytes After Paraffin Embedding

In three papers published on pituicytes, of the ox by Bucy (1930), of the human by Shanklin (1940), and of the horse by Vazquez-Lopez (1942) the pituitaries were sectioned by the freezing method and stained by the Hortega silver carbonate technic. Since that time, as a routine procedure in our laboratory, frozen sections have been replaced by paraffin which in no way interferes with the Hortega silver carbonate staining.     

Staining Myelin, Elastic Fibers and Nuclei with Iron Hematoxylin

Two iron hematoxylin staining procedures were developed. Both use stable stock solutions and can be prepared volumetrically. The nuclear stain is progressive but differentiation is required for myelin sheath and elastic tissue staining. Histochemical procedures demonstrated that acid, hydroxyl, and aldehyde groups play no role in the staining but amine groups are essential. With both types of stains neither electrostatic bonding nor hydrogen bonding is essential but the nature of the union between tissue and the iron hematoxylin complex was not determined.     

Staining Paraffin Sections with Protargol 3. The Optimum pH for Reduction. 4. A Two-Hour Staining Method

Further work on conditions affecting the reduction of paraffin sections impregnated with protargol showed that the optimum pH for sulfite-amidol mixtures was between 6.5 and 7.5. A staining method which requires about two hours to complete consists of the following steps: (1) One hour impregnation at 60° C. in 10% AgNO₃. (2) Wash in distilled water 3 changes of 30 sec. each. (3) Put into protargol (Winthrop Chem. Co., New York, N. Y.) 0.2% aq. for another hour at room temperature. (4) Rinse 2 sec. (5) Reduce one to two min. in amidol 0.2 g., Na₂SO₃ 8 g., NaHSO₃ I g., and water 100 cc. (6) Wash thoroly. (7) Tone with 0.1% gold chloride. (8) Wash. (9) Reduce with a 0.5% aq. soln. of amidol (no sulfite). (10) Wash, dehydrate and cover. The method stains neurofibrillae and unmyelinated fibers and has worked well on most tissues of vertebrates. The stain follows acid alcoholic fixation.     

Staining Mycobacterium Leprae in Paraffin Sections by the Gomori Methenamine-Silver Method

Gomori's methenamine-silver method can be used successfully for staining Mycobacterium leprae in paraffin sections even though these sections are obtained from 10-yr-old blocks, in which the acid-fastness of the microorganisms no longer exists.