共查询到18条相似文献,搜索用时 46 毫秒
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以湖北双蝴蝶带芽茎段、不带芽茎段及叶片为外植体,以MS为基本培养基,通过添加不同的植物生长物质种类和浓度配比,建立湖北双蝴蝶组培快繁体系。结果如下:在所有实验方案中,带芽茎段的出愈率最高,是理想的离体快繁材料。较适宜的初代培养基为MS+BA2.0mg/L+蔗糖3.0%,增殖培养基为MS+BA2.0mg/L+NAA0.1mg/L+蔗糖3.0%,而根的诱导则在1/2MS+NAA0.5mg/L+蔗糖1.5%的培养基上进行较为适宜。同时对组织培养过程中湖北双蝴蝶植株再生的方式进行了讨论。 相似文献
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本文运用常规手段观察了中国鹅掌楸的双受精和胚胎发生,并探讨了其致濒的原因;中国鹅掌楸的卵细胞极性化不明显,中央细胞的两极核直至受精才与精核同时融合,受精为有丝分裂前型,原始细胞型胚乳,胚胎发生为柳叶菜型。 相似文献
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中国鹅掌楸双受精和胚胎发生的细胞形态学观察 总被引:8,自引:0,他引:8
本文运用常规手段观察了中国鹅掌楸的双受精和胚胎发生,并探讨了其致濒的原因。中国鹅掌楸的卵细胞极性化不明显,中央细胞的两极核直至受精才与精核同时融合,受精为有丝分裂前型;原始细胞型胚乳,胚胎发生为柳叶菜型。 相似文献
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西洋参组织培养中的胚胎发生 总被引:6,自引:0,他引:6
西洋参(Panax quinquefolium)与人参同属,是从国外引入的名贵强壮滋补药。在组织培养方面,曾用主根、嫩茎、叶片、种胚、花药等为材料获得过愈伤组织。近年来,孙国栋等相继报道,从西洋参组织培养中可以通过器官发生途径或胚胎发生途径得到再生植株。但是,关于西洋参组织培养中的胚胎发生过程,目前尚无详细报道及确切的细胞组织学证据。为此,本文在孙国栋等工作的基础上,对西洋参种胚培养中的愈伤组织诱导及胚状体形成过程作一简要报道,以供今后进一步研究时参考。 相似文献
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植物胚胎发生过程的基因表达 总被引:1,自引:1,他引:1
植物胚胎发生过程的基因表达黄绍兴,阎隆飞(北京农业大学生物学院,北京100094)GENEEXPRESSIONDURINGPLANTEMBRYOGENESIS¥HuangShao-xing;YanLong-fei(ColleseofBiologica... 相似文献
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龙牙楤木[Aralia elate(Miq.)Seem]是五加科楤木属的多年生乔本药用植物,又称刺老鸦,具有补气、活血、祛风、利湿、止痛等功效。主要分布于我国东北地区、朝鲜、日本和俄罗斯的西伯利亚地区。其幼嫩茎叶是有名的山菜,在我国及东南亚一些国家很受欢迎。但是,近些年来由于人为恶性采伐,使 相似文献
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本研究以4年生华重楼(Paris polyphylla Smith var. chinensis(Franch.)Hara)为实验材料,分别采用0、5、20、80、160 mg/L的加镧营养液培养华重楼植株,通过比较不同营养生长指标及采用HPLC法测定根茎皂苷组分,研究不同施镧水平对华重楼生长及皂苷含量的影响。结果显示,镧对华重楼生长及皂苷含量均有显著影响,随着镧溶液浓度的增加,华重楼皂苷含量呈上升趋势,但当镧含量达到160.00 mg/L时,则会对华重楼的生长产生抑制作用,导致株高降低、比叶面积升高。研究结果表明80.00 mg/L的施镧水平最有利于华重楼的营养生长及根茎皂苷积累。 相似文献
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亚贡叶中营养成分和功能性化学成分分析 总被引:17,自引:0,他引:17
亚贡[Smallanthus sonchifolius (Poepp. et Endl.) H. Robinson]为多年生草本植物[1],原产南美洲安第斯山脉,是当地印第安人的传统根茎食品, 在我国海南、台湾等地有种植.亚贡生长在亚热带气候、无霜冻、湿度大、常年云雾缭绕且海拔1 000 m以上的高山中.亚贡可生长至2~3 m,根茎在生长7~12个月后成熟.根茎中果寡糖含量丰富[2],其低热量、高甜度的特性适合糖尿病人及减肥者食用[3]. 相似文献
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Comparative analysis of callus formation and regeneration on cultured immature maize embryos of the inbred lines A188 and A632 总被引:3,自引:0,他引:3
F.B.F. Bronsema W.J.F. van Oostveen A.A.M. van Lammeren 《Plant Cell, Tissue and Organ Culture》1997,50(1):57-65
Induction, maintenance, differentiation and embryogenic capacity of callus obtained from immature embryos by culture on induction
medium, proliferation medium, maturation medium and regeneration medium, respectively, were compared for two inbred lines
of maize, i.e. A188 and A632. The callus of inbred line A188 was embryogenic and maintained embryogenic capacity for at least
1 year. Immature embryos of inbred line A632 formed callus that was not embryogenic. It only produced roots. When sucrose
was replaced by sorbitol to induce or improve embryogenesis, again only A188 formed embryogenic callus. Subculture of this
callus, however, allowed 4 week intervals in stead of 2 week intervals without loss of embryogenic capacity.
When A188 was pollinated with A632 pollen, embryogenic callus was obtained from cultured immature "F1" embryos, showing that embryogenic capacity was inherited, maternally. The callus did not differ from the embryogenic callus
generated on selfed A188 embryos. When A632 was pollinated with A188 pollen, embryogenic callus was obtained too, showing
that embryogenic capacity was also inherited paternally, though the embryogenic capacity diminished quickly, and the stability
of the callus was lower than in the reciprocal cross.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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Embryogenic callus cultures were obtained upon repeated sub-culture of non-embryogenic callus from nodal segments of Cymbopogon martinii (Roxb.) Wats. Murashige and Skoog's medium supplemented with 1mg/l 2,4-dichlorophenoxyacetic acid and 0.5 mg/l kinetin and Linsmaier and Skoog's medium supplemented with 2mg/l 2,4-dichlorophenoxyacetic acid and 0.4 mg/l kinetin were used as maintenance media for non-embryogenic and embryogenic cultures, respectively. Plant regeneration occurred through organogenesis in MS basal media containing 2 mg/l kinetin, 1 mg/l 6-benzylaminopurine, 0.2 mg/l biotin, 0.2 mg/l Ca-pantothonate and 0.1 mg/l napthalene acetic acid. Embryogenesis was induced in LS medium supplemented with 1 mg/l kinetin, 0.5 mg/l 6-benzylaminopurine and 0.1 mg/l 3-indole acetic acid. Plant regeneration at high frequency was recorded both through organogenesis and embryogenesis in different passages of long term callus cultures.Abbreviation MS Murashige and Skoog medium - LS Linsmair and Skoog medium - BAP 6-benzylaminopurine - kin kinetin - 2,4-D 2,4-Dichlorophenoxyacetic acid - IAA Indole-3-acetic acid - CH Casein hydrolysate - CaP calcium pantothonate - NAA napthalene acetic acid 相似文献