Be more specific! Laser-assisted microdissection of plant cells

Laser-assisted microdissection (LAM) is a powerful tool for isolating specific tissues, cell types and even organelles from sectioned biological specimen in a manner conducive to the extraction of RNA, DNA or protein. LAM, which is an established technique in many areas of biology, has now been successfully adapted for use with plant tissues. Here, we provide an overview of the processes involved in conducting a successful LAM study in plants and review recent developments that have made this technique even more desirable. We also discuss how the technology might be exploited to answer some pertinent questions in plant biology.     

"Tie" calorimetry: theory and experiment

The results of theoretical and experimental investigations of the interaction of ligands to DNA permit to suggest the method of "tie" calorimetry by means of which the thermodynamic parameters of the cooperative transitions of DNA such as helix-coil, B-A and B-Z transitions were obtained. The prominence of the method is that the experimental samples contain such concentration of DNA that intermolecular interaction may be neglected. Moreover the smaller is the magnitude of the measured parameter the greater is the accuracy of the experimentally obtained data.     

"HURP on" we're off to the kinetochore!

RanGTP has a central role in spindle assembly, but the Ran-regulated factors required to initiate spindle bipolarity and stabilize MT growth toward the chromosomes remain unknown. However, three recent papers (Koffa et al., 2006; Sillje et al., 2006; Wong and Fang, 2006) have identified a single factor, HURP, that may encompass both of these properties.     

“测交实验”的分析与教学建议

从实验背景、科学方法等方面对“测交实验”进行了深入分析,实验背景可以较好地让学生从发展的角度认识科学的本质：演绎推理的学习及训练可以较好地培养学生科学探究能力。在此基础上提出了基于三维教学目标的教学建议。     

Learning to Be an Anthropologist and Remaining "Native."

Learning to Be an Anthropologist and Remaining "Native." Beatrice Medicine with Sue-Ellen Jacobs, ed. Champaign: University of Illinois Press, 2001. 371 pp.     

"To Be or Not to Be" Protonated: Atomic Details of Human Carbonic Anhydrase-Clinical Drug Complexes by Neutron Crystallography and Simulation

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