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F Lori F di Marzo Veronese A L de Vico P Lusso M S Reitz Jr R C Gallo 《Journal of virology》1992,66(8):5067-5074
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Intracellular vesicular stomatitis virus leader RNAs are found in nucleocapsid structures. 总被引:13,自引:10,他引:3
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Previous studies demonstrated that cytoplasmic extracts of cells infected with vesicular stomatitis virus contain plus-strand leader RNAs which sediment at 18S on sucrose gradients as a complex with viral N protein. The work presented in this paper demonstrated that these 18S complexes were stable on CsCl density gradients, banding at a buoyant density near that of genome nucleocapsids, and exhibited a morphology in an electron microscope similar to the disk structures found in virus genome nucleocapsids. Minus-strand leader RNAs were also found in 18S complexes on sucrose gradients. Quantitation of intracellular leader RNA suggested that, late in infection, approximately three-quarters of total intracellular leader RNA was encapsidated. 相似文献