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1.
A Genetic linkage map of Pinyon pine (Pinus edulis) based on amplified fragment length polymorphisms 总被引:1,自引:0,他引:1
S. E. Travis K. Ritland T. G. Whitham P. Keim 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):871-880
Amplified fragment length polymorphisms (AFLP) were used to rapidly generate a dense linkage map for pinyon pine (Pinus edulis). The map population consisted of 40 megagametophytes derived from one tree at Sunset Crater, Arizona. A total of 78 primer
combinations, each with three to five selective nucleotides, amplified 542 polymorphic markers. Of these, 33 markers showed
significant deviation from the expected Mendelian genotypic segregation ratio of 1 : 1, and 164 showed complete linkage with
another marker. This resulted in 338 unique markers mapping to 25 linkage groups, each of which ranged from 2 to 22 markers,
averaging 80 centiMorgans (cM) in size and covering 2,012 cM (2,200 cM with the inclusion of 25 cM for each of 7 unlinked
markers). Pairwise linkage values gave a genome size estimate of 2,390 cM, suggesting comprehensive coverage of the genome.
A search for subsets of primer combinations giving the best map coverage found 10 primer combinations which together marked
72% of the linkage map to within 10 cM; an additional 10 primer combinations increased this percentage to 85%. Our map represents
an initial step towards the identification of quantitative trait loci associated with pest resistance and water stress in
pinyons and will further allow us to examine introgression rates between P. edulis and P. californiarum.
Received: 14 October 1997 / Accepted: 29 April 1998 相似文献
2.
A genetic linkage map of cowpea (Vigna unguiculata) developed from a cross between two inbred, domesticated lines 总被引:1,自引:0,他引:1
C. M. Menéndez A. E. Hall P. Gepts 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(8):1210-1217
We have constructed a genetic linkage map within the cultivated gene pool of cowpea (2n=2x=22) from an F8 recombinant inbred population (94 individuals) derived from a cross between the inbreds IT84S-2049 and 524B. These breeding
lines, developed in Nigeria and California, show contrasting reactions against several pests and diseases and differ in several
morphological traits. Parental lines were screened with 332 random RAPD decamers, 74 RFLP probes (bean, cowpea and mung bean
genomic DNA clones), and 17 AFLP primer combinations. RAPD primers were twice as efficient as AFLP primers and RFLP probes
in detecting polymorphisms in this cross. The map consists of 181 loci, comprising 133 RAPDs, 19 RFLPs, 25 AFLPs, three morphological/classical
markers, and a biochemical marker (dehydrin). These markers identified 12 linkage groups spanning 972 cM with an average distance
of 6.4 cM between markers. Linkage groups ranged from 3 to 257 cM in length and included from 2 to 41 markers, respectively.
A gene for earliness was mapped on linkage group 2. Seed weight showed a significant association with a RAPD marker on linkage
group 5. This map should facilitate the identification of markers that “tag” genes for pest and disease resistance and other
traits in the cultivated gene pool of cowpea.
Received: 16 September 1996 / Accepted: 25 April 1997 相似文献
3.
To map the QTLsof Fusarium moniliforme ear rot resistance inZea mays L., a total of 230 F2 individuals, derived from a single cross between inbred maize lines R15 (resistant) and Ye478 (susceptible), were genotyped
for genetic map construction using simple sequence repeat (SSR) markers and amplified fragment length polymorphism (AFLP)
markers. We used 778 pairs of SSR primers and 63 combinations of AFLP primers to detect the polymorphisms between parents,
R15 and Ye478. From the polymorphic 30 AFLP primer combinations and 159 SSR primers, we scored 260 loci in the F2 population, among which 8 SSR and 13 AFLP loci could not be assigned to any of the linkage groups. An integrated molecular
genetic linkage map was constructed by the remaining 151 SSR and 88 AFLP markers, which distributed throughout the 10 linkage
groups of maize and spanned the genome of about 3463.5 cM with an average of 14.5 cM between two markers. On 4 chromosomes,
we detected 5 putative segregation distortion regions (SDRs), including 2 new ones (SDR2 and SDR7). The other 3 SDRs were located near the regions where gametophyte genes were mapped, indicating that segregation distortion could be partially
caused by gametophytic factors. 相似文献
4.
Zhigang Wei Kaixuan Zhang Chuanping Yang Guifeng Liu Guanjun Liu Lian Lian Hanguo Zhang 《Plant Molecular Biology Reporter》2010,28(1):169-175
Two separate genetic linkage maps for Chinese silver birch based on inter-simple sequence repeat (ISSR) and amplified fragment-length
polymorphism (AFLP) were constructed by a pseudo-testcross mapping strategy. Eighty F1 progenies were obtained from the cross between two parental trees with desirable traits (the paternal one selected from ‘Qinghai’
and the maternal one from ‘Wangqing’). A total of 46 ISSR primers and 31 AFLP primers were employed to generate 102 ISSR and
355 AFLP polymorphic markers in the F1 progenies. About 5.7% of all the markers displayed high segregation distortion with a P value below 0.01 and such markers were not used for map constructions. The paternal map consisted of 137 loci, spread over
13 groups and spanned 694.2 cM at an average distance of 5.1 cM between the markers, while in the maternal map, 147 loci were
distributed in 14 groups covering a map distance about 949.62 cM at an average distance of 6.5 cM. These initial maps can
serve as the basis for developing a more detailed genetic map. 相似文献
5.
The development of a genetic map for meadowfoam comprised of amplified fragment length polymorphisms
Katengam S Crane JM Knapp SJ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(1):92-96
Limnanthes alba Benth. (meadowfoam), a diploid (x=5) winter annual, produces novel very long-chain seed oils (C20 and C22) with less than 2% saturated fatty acids. The first genetic map of meadowfoam, a recently domesticated species, is described
herein. Two phenotypically diverse inbred lines, OMF40–11 (L. alba ssp. alba) and OMF64 (L. alba ssp. versicolor), were screened for amplified fragment length polymorphisms (AFLPs) using 16 primer combinations. Twenty three percent of
the AFLP bands (415 out of 1,801) were polymorphic between OMF40–11 and OMF64. One hundred (OMF40–11×OMF64)×OMF64 BC1 progeny were genotyped for 107 polymorphic AFLP markers produced by nine AFLP primer combinations. One hundred and three
AFLP loci amalgamated into five linkage groups with 14 to 28 loci per linkage group (four loci segregated independently).
The map was 698.5-cM long with a mean interlocus spacing of 6.7 cM and no dense clustering of loci. The segregation ratios
for 25 loci (23.2%) were significantly distorted. Twenty one of the distorted loci (84%) had an excess of L. alba ssp. versicolor (recurrent parent) alleles. The distorted loci, apart from one locus on linkage group 4, were distally clustered on both
ends of linkage groups 1, 4 and 5. The development of the map was facilitated by the small chromosome number, an abundance
of restriction site polymorphisms between the two subspecies (23%), and a high multiplex ratio of the AFLP markers (112 per
primer combination).
Received: 16 October 2000 / Accepted: 18 April 2001 相似文献
6.
Fuli Liu Xiuliang Wang Jidong Liu Wandong Fu Delin Duan Yingxia Yang 《Journal of phycology》2009,45(5):1228-1233
To establish a molecular‐marker‐assisted system of breeding and genetic study for Laminaria japonica Aresch., amplified fragment length polymorphism (AFLP) was used to construct a genetic linkage map of L. japonica featuring 230 progeny of F2 cross population. Eighteen primer combinations produced 370 polymorphic loci and 215 polymorphic loci segregated in a 3:1 Mendelian segregation ratio (P ≤ 0.05). Of the 215 segregated loci, 142 were ordered into 27 linkage groups. The length of the linkage groups ranged from 6.7 to 90.3 centimorgans (cM) with an average length of 49.6 cM, and the total length was 1,085.8 cM, which covered 68.4% of the estimated 1,586.9 cM genome. The number of mapped markers on each linkage group ranged from 2 to 12, averaging 5.3 markers per group. The average density of the markers was 1 per 9.4 cM. Based on the marker density and the resolution of the map, the constructed linkage map can satisfy the need for quantitative trait locus (QTL) location and molecular‐marker‐assisted breeding for Laminaria. 相似文献
7.
AFLP genetic maps of Eucalyptus globulus and E. tereticornis 总被引:8,自引:0,他引:8
C. M. Marques J. A. Araújo J. G. Ferreira R. Whetten D. M. O’Malley B.-H. Liu R. Sederoff 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(6-7):727-737
Amplified fragment length polymorphism (AFLP) analysis is a rapid and efficient technique for detecting large numbers of
DNA markers in eucalypts. We have used AFLP markers in a two-way pseudo-testcross strategy to generate genetic maps of two
clones of different Eucalyptus species (E. tereticornis and E. globulus). Of 606 polymorphic fragments scored, 487 segregated in a 1 : 1 ratio, corresponding to DNA polymorphisms heterozygous in
one parent and null in the other. In the maternal E. tereticornis map, 268 markers were ordered in 14 linkage groups (919 cM); the paternal E. globulus map had 200 markers in 16 linkage groups (967 cM). Results from PGRI software were compared with MAPMAKER. The average density
of markers was approximately 1 per 3.9 cM. Framework markers were ordered with an average confidence level of 90%, covering
80–100% of the estimated Eucalyptus genome size. In order to investigate the homologies between the E. tereticornis and the E. globulus genetic linkage maps, we included 19 markers segregating 3 : 1 in the analysis. Some homeologous linkage groups were recognized.
The linkage data developed in these maps will be used to detect loci controlling commercially important traits.
Received: 17 July 1997 / Accepted: 13 October 1997 相似文献
8.
Construction of an RFLP linkage map for cultivated sunflower 总被引:5,自引:0,他引:5
C. C. Jan B. A. Vick J. F. Miller A. L. Kahler E. T. Butler III. 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):15-22
An RFLP linkage map was constructed for cultivated sunflower Helianthus annuus L., based on 271 loci detected by 232 cDNA probes. Ninety-three F2 plants of a cross between inbred lines RHA 271 and HA 234 were used as the mapping population. These genetic markers plus
a fertility restoration gene, Rf
1, defined 20 linkage groups, covering 1164 cM of the sunflower genome. Of the 71 loci 202 had codominant genotypic segregation,
with the rest showing dominant segregation. Thirty-two of the 232 probes gave multiple locus segregation. There were 39 clusters
of tightly linked markers with 0 cM distance among loci. This map has an average marker-to-marker distance of 4.6 cM, with
11 markerless regions exceeding 20 cM.
Received: 17 June 1997 / Accepted: 19 June 1997 相似文献
9.
Preliminary genetic linkage maps were constructed for the Pacific abalone (Haliotis discus hannai Ino) using amplified fragment length polymorphism (AFLP), randomly amplified polymorphic DNA (RAPD), and microsatellite markers
segregating in a F1 family. Nine microsatellite loci, 41 RAPD, and 2688 AFLP markers were genotyped in the parents and 86 progeny of the mapping
family. Among the 2738 markers, 384 (including 365 AFLP markers, 10 RAPD markers, and 9 microsatellite loci) were polymorphic
and segregated in one or both parents: 241 in the female and 146 in the male. The majority of these markers, 232 in the female
and 134 in the male, segregated according to the expected 1:1 Mendelian ratio (α = 0.05). Two genetic linkage maps were constructed
using markers segregating in the female or the male parent. The female framework map consisted of 119 markers in 22 linkage
groups, covering 1773.6 cM with an average intermarker space of 18.3 cM. The male framework map contained 94 markers in 19
linkage groups, spanning 1365.9 cM with an average intermarker space of 18.2 cM. The sex determination locus was mapped to
the male map but not to the female map, suggesting a XY-male determination mechanism. Distorted markers showing excess of
homozygotes were mapped in clusters, probably because of their linkage to a gene that is incompatible between two parental
populations. 相似文献
10.
A genetic linkage map of tef [Eragrostis tef (Zucc.) Trotter] based on amplified fragment length polymorphism 总被引:2,自引:0,他引:2
G. Bai H. Tefera M. Ayele H. T. Nguyen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(3-4):599-604
A genetic linkage map of tef was constructed with amplified fragment length polymorphism (AFLP) markers using F5 recombinant inbred lines (RILs) derived by single seed descent from the intraspecific cross of ’Kaye Murri’×’Fesho’. A total
of 192 EcoRI/MseI primer combinations were screened for parental polymorphism. Around three polymorphic fragments per primer combination were
detected, indicating a low polymorphism level in tef. Fifty primer combinations were selected to assay the mapping population,
and 226 loci segregated among 85 F5 RILs. Most AFLP loci behaved as dominant markers (presence or absence of a band), but about 15% of the loci were codominant.
Significant deviations from the expected Mendelian segregation ratio were observed for 26 loci. The genetic linkage map comprised
211 markers assembled into 25 linkage groups and covered 2,149 cM of genome. AFLP is an efficient marker system for mapping
plant species with low polymorphism such as tef. This is the first genetic linkage map constructed for tef. It will facilitate
the mapping of genes controlling agronomically important traits and cultivar improvement in tef.
Received: 27 April 1998 / Accepted: 4 January 1999 相似文献
11.
W. Y. Cheung L. Friesen G. F. W. Rakow G. Séguin-Swartz B. S. Landry 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(6-7):841-851
A genetic linkage map of Brassica juncea was constructed based on restriction fragment length polymorphism (RFLP) detected by anonymous cDNA markers from B. napus, using a segregating F1-derived doubled haploid (DH) progeny from a cross between a canola-quality mustard line (J90-4317) and a high-oil-content
mustard line (J90-2733). The RFLP probes consisted of 229 cDNA probes from B. napus and a B. napus tandem repeat sequence, RDA2. The map consisted of 343 marker loci arranged in 18 major linkage groups plus five small segments
with two to five marker loci, covering a total map distance of 2073 cM. Twenty-four percent of the markers were dominant in
nature. Sixty-two percent of the marker loci were duplicated, and the majority were involved in inter-linkage group duplications,
illustrating that complex duplications and subsequent rearrangements occurred after allopolyploidy. Deviation from the Mendelian
segregation ratio for a DH population was observed for 27% of the markers. Two-thirds of these markers with a skewed segregation
were clustered in 6 linkage groups and two unassigned segments. The overall average marker interval of the B. juncea map reported here was 6.6 cM, which would provide a marker density satisfactory for efficient use of the map in breeding
applications, such as tagging of important agronomic traits and marker-assisted selection.
Received: 14 May 1996 / Accepted: 11 October 1996 相似文献
12.
Verde I Lauria M Dettori MT Vendramin E Balconi C Micali S Wang Y Marrazzo MT Cipriani G Hartings H Testolin R Abbott AG Motto M Quarta R 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(6):1013-1021
A set of 146 single sequence repeats (SSRs) and 14 amplified fragment length polymorphism (AFLP) primer combinations were used to enrich a previously developed linkage map obtained from a (Prunus persica×P. ferganensis)×P. persica BC1 progeny. Forty-one SSR primer pairs gave polymorphic patterns detecting 42 loci. The restriction/selective primer AFLP combinations produced a total of 79 segregating fragments. The resulting map is composed of 216 loci covering 665 cM with an average distance of 3.1 cM. Novel regions were covered by the newly mapped loci for a total of 159 cM. Eight linkage groups were assembled instead of the earlier 10 as two small groups (G1a and G8b), previously independent, were joined to their respective major groups (G1b and G8a). Several gaps were also reduced resulting in an improved saturation of the map. Twelve gaps ≥10 cm are still present. A comparative analysis against the Prunus reference map (71 anchor loci) pointed out an almost complete synteny and colinearity. Six loci were not syntenic and only two were not colinear. Genetic distances were significantly longer in our map than in the reference one. 相似文献
13.
R. N. Morgan J. P. Wilson W. W. Hanna P. Ozias-Akins 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(3-4):413-420
Pearl millet [Pennisetum glaucum (L.) R.Br.] is a warm-season grass used for food, feed, fodder and forage, primarily in countries of Africa and India but
grown around the world. The two most-destructive diseases to pearl millet in the United States are rust (caused by Puccinia substriata var. indica) and pyricularia leaf spot (caused by Pyricularia grisea). Genes for disease resistance to both pathogens have been transferred into agronomically acceptable forage and grain cultivars.
A study was undertaken to identify molecular markers for three rust loci and one pyricularia resistance locus. Three segregating
populations were screened for RAPDs using random decamer primers and for RFLPs using a core set of probes detecting single-copy
markers on the pearl millet map. The rust resistance gene Rr
1
from the pearl millet subspecies P. glaucum ssp. monodii was linked 8.5 cM from the RAPD OP-G8350. The linkage of two RFLP markers, Xpsm108 (15.5 cM) and Xpsm174 (17.7 cM), placed the Rr
1
gene on linkage-group 3 of the pearl millet map. Rust resistance genes from both Tift 89D2 and ICMP 83506 were placed on linkage-group 4 by determining genetic linkage to the RFLP marker Xpsm716 (4.9 and 0.0 cM, respectively). Resistance in ICMP 83506 was also linked to the RFLP marker Xpsm306 (10.0 cM), while resistance in Tift 89D2 was linked to RAPD markers OP-K19350 (8.8 cM) and OP-O8350 (19.6 cM). Fragments from OP-K19 and OP-O8 in the ICMP 83506 population, and Xpsm306 in the Tift 89D2 population, were monomorphic. Only one RAPD marker (OP-D11700, 5.6 cM) was linked to pyricularia leaf spot resistance. Attempts to detect polymorphisms with rice RFLP probes linked to
rice blast resistance (Pyricularia oryzae; syn=P. grisea) were unsuccessful.
Received: 19 May 1997 / Accepted: 21 October 1997 相似文献
14.
Tingbo Jiang Boru Zhou Fuling Gao Baozhu Guo 《Molecular breeding : new strategies in plant improvement》2011,27(3):347-356
A pseudo-testcross mapping strategy was used in combination with the random amplified polymorphism DNA (RAPD) and amplified
fragment length polymorphism (AFLP) genotyping methods to develop two moderately dense genetic linkage maps for Betula platyphylla Suk. (Asian white birch) and B. pendula Roth (European white birch). Eighty F1 progenies were screened with 291 RAPD markers and 451 AFLP markers. We selected 230 RAPD and 362 AFLP markers with 1:1 segregation
and used them for constructing the parent-specific linkage maps. The resultant map for B. platyphylla was composed of 226 markers in 24 linkage groups (LGs), and spanned 2864.5 cM with an average of 14.3 cM between adjacent
markers. The linkage map for B. pendula was composed of 226 markers in 23 LGs, covering 2489.7 cM. The average map distance between adjacent markers was 13.1 cM.
Clustering of AFLP markers was observed on several LGs. The availability of these white birch linkage maps will contribute
to the molecular genetics and the implementation of marker-assisted selection in these important forest species. 相似文献
15.
J. P. W. Haanstra C. Wye H. Verbakel F. Meijer-Dekens P. van den Berg P. Odinot A. W. van Heusden S. Tanksley P. Lindhout J. Peleman 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):254-271
Two independent F2 populations of Lycopersicon esculentum×L. pennellii which have previously been investigated in RFLP mapping studies were used for construction of a highly saturated integrated
AFLP map. This map spanned 1482 cM and contained 67 RFLP markers, 1078 AFLP markers obtained with 22 EcoRI+MseI primer combinations and 97 AFLP markers obtained with five PstI+MseI primer combinations, 231 AFLP markers being common to both populations. The EcoRI+MseI AFLP markers were not evenly distributed over the chromosomes. Around the centromeric region, 848 EcoRI+ MseI AFLP markers were clustered and covered a genetic distance of 199 cM, corresponding to one EcoRI+ MseI AFLP marker per 0.23 cM; on the distal parts 1283 cM were covered by 230 EcoRI+MseI AFLP markers, corresponding to one marker per 5.6 cM. The PstI/MseI AFLP markers showed a more even distribution with 16 PstI/MseI AFLP markers covering a genetic distance of 199 cM around the centromeric regions and 81 PstI/MseI AFLP markers covering a genetic distance of 1283 cM on the more distal parts, corresponding to one marker per 12 and 16 cM
respectively. In both populations a large number of loci showed a significant skewed segregation, but only chromosome 10 loci
showed skewness that was similar for both populations. This ultra-dense molecular-marker map provides good perspectives for
genetic and breeding purposes and map-based cloning.
Received: 3 September 1998 / Accepted: 27 October 1998 相似文献
16.
Development of RAPD and SCAR markers linked to the Russian wheat aphid resistance gene Dn2 in wheat 总被引:5,自引:0,他引:5
A. A. Myburg M. Cawood B. D. Wingfield A.-M. Botha 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(8):1162-1169
RAPD (random amplified polymorphic DNA) analysis was used to identify molecular markers linked to the Dn2 gene conferring resistance to the Russian wheat aphid (Diuraphis noxia Mordvilko). A set of near-isogenic lines (NILs) was screened with 300 RAPD primers for polymorphisms linked to the Dn2 gene. A total of 2700 RAPD loci were screened for linkage to the resistance locus. Four polymorphic RAPD fragments, two in
coupling phase and two in repulsion phase, were identified as putative RAPD markers for the Dn2 gene. Segregation analysis of these markers in an F2 population segregating for the resistance gene revealed that all four markers were closely linked to the Dn2 locus. Linkage distances ranged from 3.3 cM to 4.4 cM. Southern analysis of the RAPD products using the cloned RAPD markers
as probes confirmed the homology of the RAPD amplification products. The coupling-phase marker OPB10880c and the repulsion-phase marker OPN1400r were converted to sequence characterized amplified region (SCAR) markers. SCAR analysis of the F2 population and other resistant and susceptible South African wheat cultivars corroborated the observed linkage of the RAPD
markers to the Dn2 resistance locus. These markers will be useful for marker-assisted selection of the Dn2 gene for resistance breeding and gene pyramiding.
Received: 1 July 1997 / Accepted: 20 October 1997 相似文献
17.
Genetic linkage map of ISSR and RAPD markers in Einkorn wheat in relation to that of RFLP markers 总被引:41,自引:0,他引:41
T. Kojima T. Nagaoka K. Noda Y. Ogihara 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):37-45
The potential of PCR-based markers for construction of a genetic linkage map in Einkorn wheat was investigated. From a comparison
of polymorphisms between two Einkorn wheats, Triticum monococcum (Mn) and T. boeoticum (Bt), we obtained 49 polymorphic bands produced by 33 primers for inter-simple sequence repeat (ISSR) and 36 polymorphic
bands shown by 25 combinations of random amplified polymorphic DNA (RAPD) primers for mapping in 66 individuals in the F2 population. Although 44 ISSR fragments and 29 RAPD fragments statistically showed a 3 : 1 segregation ratio in the F2 population, only 9 markers each of the ISSR and RAPD bands were able to be mapped on the RFLP linkage map of Einkorn wheat.
ISSR markers were distributed throughout the chromosomes. The mapped positions of the ISSR markers seemed to be similar to
those obtained by the RFLP markers. On the other hand, 4 of the 9 RAPD markers could map the RFLP marker-poor region on the
short arm of 3Am, suggesting a potential to map novel regions containing repetitive sequences. Comparisons of the genetic linkage map of Einkorn
wheat to the linkage map and cytological map of common wheat revealed that the marker orders between the two maps of Einkorn
wheat and common wheat coincided except for 4A, which harbors chromosome rearrangements specific for polyploid wheats, indicating
a conservatism between the two genomes. Recombinations in Einkorn wheat chromosomes took place more frequently around the
centromere and less at the distal part of chromosomes in comparison to those in common wheat. Nevertheless, recombinations
even in Einkorn wheat chromosomes were strongly suppressed around the centromere. In fact, the markers located within 1 cM
of the centromere were located almost in the central part of the chromosome arm.
Received: 7 June 1997 / Accepted: 17 June 1997 相似文献
18.
Yuhui Li Yingxia Yang Jidong Liu Xiuliang Wang Tianxiang Gao Delin Duan 《植物学报(英文版)》2007,49(3):392-400
With a "two-way pseudo-testcross" mapping strategy, we applied the amplified fragment length polymorphism (AFLP) markers to construct two moderate density genetic linkage maps for Laminaria. The linkage maps were generated from the 60 progenies of the F1 cross family (Laminaria iongissima Aresch. × L. Japonica Miyabe) with twenty pairs of primer combinations. Of the 333 polymorphic loci scored in 60 progenies, 173 segregated in a 1:1 ratio, corresponding to DNA polymorphisms heterozygous in a single parent, and the other 58 loci existing in both parents followed a 3:1 Mendelian segregation ratio. Among the loci with 1:1 segregating ratios, 79 loci were ordered in 14 linkage groups (648.6 cM) of the paternal map, and 72 loci were ordered in 14 linkage groups (601.9 cM) of the maternal map. The average density of loci was approximately 1 per 8 cM. To Investigate the homologies between two parental maps, we used 58 loci segregated 3:1 for further analysis, and deduced one homologous linkage group. The linkage data developed in these maps will be useful for detecting loci-controlling commercially important traits for Laminaria. 相似文献
19.
M. J. Truco C. F. Quiros 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,89(5):590-598
We constructed a genetic map on Brassica nigra based on a segregating population of 83 F2 individuals. Three different types of molecular markers were used to build the map including isozymes, restriction fragment length polymorphisms (RFLP), and random amplified polymorphic DNA (RAPD). The final map contained 124 markers distributed in 11 linkage groups. The map covered a total distance of 677 cM with the markers distributed within a mean distance of 5.5cM. Of the sequences found in the B. nigra map, 40% were duplicated and organized into three different types of arrangements. They were either scattered throughout the genome, organized in tandem, or organized in blocks of duplicated loci conserved in more than 1 linkage group. 相似文献
20.
A linkage map of the pea (Pisum sativum L.) genome containing cloned sequences of known function and expressed sequence tags (ESTs) 总被引:3,自引:0,他引:3
B. J. Gilpin J. A. McCallum T. J. Frew G. M. Timmerman-Vaughan 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(8):1289-1299
A linkage map of the pea (Pisum sativum L.) genome is presented which is based on F2 plants produced by crossing the marrowfat cultivar ‘Primo’ and the blue-pea breeding line ‘OSU442-15’. This linkage map consists
of 209 markers and covers 1330 cM (Kosambi units) and includes RFLP, RAPD and AFLP markers. By mapping a number of anchor
loci, the ‘Primo’בOSU442-15’ map has been related to other pea linkage maps. A feature of the map is the incorporation of
29 loci representing genes of known function, obtained from other laboratories. The map also contains RFLP loci detected using
sequence-characterized cDNA clones developed in our laboratory. The putative identities of 38 of these cDNA clones were assigned
by examining public-sequence databases for protein or nucleotide-sequence similarities. The conversion of sequence-characterized
pea cDNAs into PCR-amplifiable and polymorphic sequence-tagged sites (STSs) was investigated using 18 pairs of primers designed
for single-copy sequences. Eleven polymorphic STSs were developed.
Received: 18 June 1997 / Accepted: 11 August 1997 相似文献