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Heiichi Sakai Rimpei Mori Kei Arima 《Bioscience, biotechnology, and biochemistry》2013,77(10):660-678
The production and isolation of a new toxic substance, Teleocidin, and its biological properties were previously reported1,2). Thereafter it has been found that an other strain of Streptomyces produced such specific toxic substance as Teleocidin in its cultured mycellium. Comparative tests of these two purified crystalline powders showed the new toxic substance resembles Teleocidin closely though differs in certain chemical properties. Therefore, the original Teleocidin is designated Teleocidin A, whereas that produced by a new strain of Streptomyces is named Teleocidin B, which had been tentatively called as the SK-toxic substance.From the results of the chemical studies of Teleocidin B and its hydrogenated derivative, which was easily obtained as a crystalline form by the catalytic hydrogenation of Teleocidin B with Adam’s catalyst, molecular formula, C28H39~41N3O2 was postulated for Teleocidin B.It was also recognized that an alcoholic hydroxyl, a lactam ring and a heterocyclic ring like indole or pyrrole structure existed as the functional groups of Teleocidin B. 相似文献
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Carmen Verissima Ferreira Eulazio Mikio Taga Hiroshi Aoyama 《Journal of enzyme inhibition and medicinal chemistry》2013,28(4):403-410
The four soybean seed acid phosphatase isoforms AP1, AP2, AP3A and AP3B were competitively inhibited by phosphate, vanadate, fluoride and molybdate, using p-nitrophenylphos-phate as substrate. The four isoforms were not significantly affected by compounds that can interact with SH residues or by pyridoxal phosphate. These results indicated that cysteine and lysine residues are not present in the active site of the four soybean seed acid phosphatase isoforms. The inhibition constant values for phosphate, vanadate, fluoride and molybdate at pH 5.0 were respectively: API (250, 12.8, 1.7, 0.05 μM), AP2 (800,10, 500, 0.025 μM), AP3A (250, 24.2,250, 0.032 μM), AP3B (2400, 36.9,750, 0.05 μM). 相似文献
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Transformation of Soybean (Glycine max) by Infecting Germinating Seeds with Agrobacterium tumefaciens 总被引:8,自引:1,他引:8 下载免费PDF全文
The transfer of genetic material into soybean tissue was accomplished by using an avirulent strain of Agrobacterium tumefaciens which contained the binary vector pGA482. The method used for transformation requires no tissue culture steps as it involves the inoculation of the plumule, cotyledonary node, and adjacent cotyledon tissues of germinating seeds. The identification of neomycin phosphotransferase (NPT) II enzyme activity in the tissues of 16 (R0) soybean plants indicated that the plant expressible Nos-NPT II gene, contained within the T-DNA region from pGA482, had been transferred at least into somatic tissues. Putative transformed R0 soybean plants were advanced to produce R1 plants which were also assayed for the presence of the transferred Nos-NPT II gene. The combined results of these assays indicated that about 0.7% of the surviving inoculated seeds yielded transformed tissues in the R0 plant, and that about 1/10 of these plants yielded transformed R1 plants. The presence of the Nos-NPT II gene in DNAs isolated from both R0 and R1 plant was demonstrated by using genomic blot hybridization and polymerase chain reaction methods. Integration of this gene into the soybean genome was demonstrated for three R1 soybean plants. 相似文献
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In the course of studies on the screening of specific toxic substances produced by Streptomyces against aquatic organisms, it was recognised that a newly isolated Streptmyces, 2A 1563, which was considered to be a variant species of Streptomyces mediocidicus produces a new toxic substance, Teleocidin. Teleocidin was isolated as a white powder resembling crystals with a crystal appearance from the methanol extracts of the cultured mycellium, and showed a specific toxic action toward Japanese killifish and mice, but did not exhibit any inhibition against microorganisms. 相似文献
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Shigemitsu Kudou Yvette Fleury Dieter Welti Daniele Magnolato Teiji Uchida Keisuke Kitamura 《Bioscience, biotechnology, and biochemistry》2013,77(9):2227-2233
The isoflavone constituents in soybean seeds were investigated, and 9 kinds of isoflavone glycosides were isolated from the hypocotyls of soybean seeds. Three kinds were proved to be malonylated soybean isoflavones named 6″-O-malonyldaidzin, 6″-O-malonylglycitin and 6″-O-malonylgenistin by UV, MS, IR and NMR. The malonylated isoflavone glycosides as major isoflavone constituents in soybean seed were thermally unstable, and were converted into their corresponding isoflavone glycosides. All of the isoflavone components produced intensely undesirable taste effects such as bitter, astringent and dry mouth feeling. 相似文献
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野生大豆种子cDNA文库的构建与分析 总被引:4,自引:0,他引:4
为了分离与鉴定野生大豆优良基因,以双高型优质野生大豆的近成熟种子为材料,采用裂解法提取了总RNA;以Oligo(dT)为引物,经SA—PMPS法分离出mRNA,反转录酶催化合成cDNA,并以cDNA第一链为模板在DNA聚合酶Ⅰ的作用下合成cDNA第二链,双链cDNA经加接头等步骤,成功构建了野生大豆cDNA文库。文库的重组率约为93.7%,PCR检测重组克隆的插入片段平均大于1000bp,测序片断大于500bp,表明构建的近成熟种子cDNA文库质量较高,为进一步进行EST测序和全长克隆打下了基础。 相似文献
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Characterization of Solute Efflux from Dehydration Injured Soybean (Glycine max L. Merr) Seeds 总被引:1,自引:5,他引:1 下载免费PDF全文
Soybean (Glycine max L. Merr) seeds lose their tolerance of dehydration between 6 and 36 hours of imbibition. Soybean axes and cotyledons were excised 6 hours (tolerant of dehydration) and 36 hours (susceptible) after commencing imbibition and subsequently dehydrated to 10% moisture. Kinetics of the efflux of potassium, phosphate, amino acid, sugar, protein, and total electrolytes were compared in the four treatments during rehydration. Only slight differences were observed in the kinetics of solute efflux between the two cotyledon treatments dehydrated at 6 and 36 hours suggesting that the cotyledons may retain their tolerance of dehydration at this stage of germination. Several symptoms of injury were observed in the axes dehydrated at 36 hours. An increase in the initial leakage of solutes during rehydration, as quantified by the y-intercept of the linear regression line for solute efflux between 2 and 8 hours suggests an increased incidence of cell rupture. An increase in the rate of solute efflux (slope of regression line between 2 and 8 hours) from fully rehydrated axes was observed in comparison to axes dehydrated at 6 hours. The Arrhenius activation energy for potassium, phosphate, and amino acid efflux decreased and for protein remained unchanged. Both observations indicate an increase in membrane permeability in dehydration-injured tissue. Increasing the H+ concentration of the external solution increased K+ efflux from both control and dehydrated/rehydrated samples, increased sugar efflux from axes at 6 hours imbibition but decreased sugar efflux from axes at 36 hours imbibition, indicating changes in membrane properties during germination. The dehydration treatment did not alter the pattern of the pH response of axes dehydrated at 6 or 36 hours but did increase the quantity of potassium and sugar efflux from dehydration injured axes. These results are interpreted as indicating that dehydration of soybean axes at 36 hours of imbibition increased both the incidence of cell rupture during rehydration and altered membrane permeability of the rehydrated tissue. 相似文献
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The aim of this study was to investigate whether there were differences in the genetic variability and rate and velocity of
the seed germination produced by Psychotria tenuinervis located at anthropogenic edges, natural edges, and in the forest interior. The populations of P. tenuinervis showed no differences in genetic variability or structure among the three habitats. There was, however, an indication of inbreeding,
which was significantly higher in natural edges than in anthropogenic edges and the forest interior. Within-habitat variation
was considerable, but there were no differences in seed mass or rate and velocity of germination among the three habitats.
These results suggest that seed characteristics were not influenced by the genetic pattern of P. tenuinervis and that other characteristics of the forest fragment, such as gaps, edge age, and type of matrix exert more influence on
seed mass and germination than the distance from the edges. 相似文献
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国产羊肚菌菌株的RAPD鉴别 总被引:4,自引:1,他引:4
对国内各地收集和采集分离到的 15个羊肚菌菌株和 1个子实体对照进行了RAPD分析 ,实验筛选的 6个引物对宽圆羊肚菌 (MorchellarobustaBond .)子实体及其分离菌株的DNA指纹完全相同 ,引物OPD - 0 8扩增的RAPD谱带能够将 15个供试羊肚菌菌株完全区别开来 ;聚类分析结果显示 :其中一菌株与别的菌株相似系数极低 ,可能为一杂菌菌株 ;其余菌株在相似系数 0 75 44时 ,可分为 6个大的类群 ,说明供试菌株间存在较大的遗传差异。 相似文献
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Fusarium graminearum and F. culmorum are the major causal agents of Fusarium head blight in Turkey. They produce trichothecenes such as deoxynivalenol (DON),
nivalenol (NIV) and their several acetylated derivatives, 3-acetyldeoxynivalenol (3-ADON) and 15-acetyldeoxynivalenol (15-ADON).
In this study, a total of thirty-three isolates of F. graminearum and F. culmorum were collected from various regions and three different hosts. They were identified by amplification of tri5 gene cluster. Totally 32 isolates, 21 of F. culmorum and 11 of F. graminearum, were determined as DON chemotype, while only one F. graminearum isolate (1F) was detected as a NIV. A 282 base pair (bp) band for tri13 gene and also ranging from 458 to 535 bp bands for tri7 gene were amplified in all DON producers’ genomes. Further analysis of DON chemotype based on tri3 gene amplification showed that all isolates of F. graminearum displayed 15-ADON sub-chemotype. They yielded a 863 bp amplicon. Similarly, 3-ADON sub-chemotype was identified in F. culmorum’ isolates except F13. As a result of tri3 gene assay, it was produced a 583 bp fragment in these twenty isolates. It is the first report that a F. graminearum isolate depicts NIV chemotype in agricultural regions of Turkey. According to our findings, DON chemotype is predominating
in our country. Also, it is presented that most of the F. graminearum isolates have 15-ADON sub-chemotype, while all F. culmorum’s belong to 3-ADON which possess full length amplicon of tri7 gene. 相似文献
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Histopathology of Oil Palm Seedlings Infected by, Pathogenic Fusarium oxysporum Isolates from Africa
Oil palm seedlings (one-leaf stage) grown from Malaysian seed were inoculated with six African isolates of Fusarium oxysporum f. sp. elaeidis and one African isolate of Fusarium oxysporum var. redolens. All the isolates induced similar symptoms and anatomical responses in the inoculated palms. The pathogen invaded the root, bulb, leaf bases and leaves in that sequence. In the root and bulb, infection resulted in plugging of xylem elements with mycelium, conidia, tyloses and gums, disintegration and plugging of phloem tissues, disintegration of plugged vascular elements forming gaps and cavities, and collapse of cortical tissues leading to the isolation of the infected vascular tissues. Formation of hypertrophic or hyperplastic cells in the xylem parenchyma was not observed. Anatomical changes in the infected leaf bases and petioles were similar to those occurring in the infected bulb; except that tyloses were formed less frequently. Generally there was no sign of the pathogen in the wilted leaf blade, but the epidermal, hypodermal and mesophyll cells appeared shrunken or to have collapsed. No hyphae or anatomical changes were observed m the tissues of symptomless inoculated palms. 相似文献
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Purification of Host DNA Synthesis-Suppressing Factor (DSF) Produced by Infection with Measles Virus
Host DNA synthesis-suppressing factor (DSF) produced into culture fluid of cloned HeLa cells (HeLa C-9) infected with a small plaque variant of Toyoshima strain of measles virus was purified by precipitation with ammonium sulfate, chromatography on CM-cellulose and DEAE-cellulose, and gel-filtration on Sephadex G-100 and G-200. The specific activity of the finally purified DSF was 302 units/mg of protein representing approximately 300-fold purification. The molecular weight of DSF was estimated to be about 55 000. By isoelectric focusing, two kinds of DSF having isoelectric points of 4.24 and 5.24 were detectable. The purified DSF was able to suppress host DNA synthesis of HeLa cells, continuous human lymphoid cells (NC-37), mouse L cells and Meth-A cells derived from an ascitic tumor of the mouse. The activity of the purified DSF was inactivated by heating at 56 C for 30 min or by treatment with trypsin. 相似文献
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Cho Eun Seob; Choi Byeong Dae; Cho Yong Chul; Kim Tae Jin; Kim Hak Gyoon 《Journal of plankton research》2001,23(1):89-96
A lectin binding assay was conducted on three Alexandrium tamarenseisolates (AT-A, AT-2 and AT-6). The fatty acid composition ofall three isolates was analyzed, and the total carotenoid contentandß-carotene were also determined. When treated withdifferent lectins in this study, AT-A and AT-2 showed a positiveresponse, whereas the potentially toxic AT-6 did not bind thelectin Dolchis biflorus agglutinin (DBA), regardless of growthphase, but conjugated concanavalin A (ConA), peanut agglutinin(PNA), Ricinus communis agglutinin (RCA), soybean agglutinin(SBA), Ulex europaeus agglutinin (UEA) and wheatgerm agglutinin(WGA). It is possible that the use of DBA is a desirable methodfor rapid and easy discrimination of highly toxic A. tamarense.AT-A, AT-2 and AT-6 comprised saturated fatty acids (49.061.9%),monounsaturated fatty acids (8.020.5%) and polyunsaturatedfatty acids (23.230.5%). In particular, 22:6 (n-3) polyunsaturatedfatty acid had a high abundance in AT-6 compared with AT-A andAT-2. However, carotenoid content and ß-carotene did notcontribute to the discrimination of each isolate. Owing to variabilityin the biochemical composition of different isolates, possiblyDBA and 22:6 (n-3) polyunsaturated fatty acid provide good informationfor the discrimination of AT-6. 相似文献
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A new isocoumarin derivative named fusariumin (1), together with two known related resorcylic acid lactones aigialomycin D (2) and pochonin N (3), has been isolated from the cultures of Fusarium sp. LN-10, an endophytic fungus originated from the leaves of Melia azedarach. Their structures were established on the basis of extensive spectroscopic analyzes including 1D- and 2D- NMR (1H-1H COSY, HSQC, HMBC, and NOESY) experiments. Compounds 1-3 displayed significant growth inhibitory activity against the brine shrimp (Artemia salina). 相似文献
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Ya-Min Chang Li-Chun Chen Hsin-Yi Wang Chui-Liang Chiang Chen-Tien Chang Yun-Chin Chung 《PloS one》2014,9(12)
Using 4-methylumbelliferyl-β-D-N,N′,N″-triacetylchitotrioside (4-MU-GlcNAc3) as a substrate, an acidic chitinase was purified from seeds of black soybean (Glycine max Tainan no. 3) by ammonium sulfate fractionation and three successive steps of column chromatography. The purified chitinase was a monomeric enzyme with molecular mass of 20.1 kDa and isoelectric point of 4.34. The enzyme catalyzed the hydrolysis of synthetic substrates p-nitrophenyl N-acetyl chitooligosaccharides with chain length from 3 to 5 (GlcNAcn, n = 3-5), and pNp-GlcNAc4 was the most degradable substrate. Using pNp-GlcNAc4 as a substrate, the optimal pH for the enzyme reaction was 4.0; kinetic parameters K
m and kcat were 245 µM and 10.31 min−1, respectively. This enzyme also showed activity toward CM-chitin-RBV, a polymer form of chitin, and N-acetyl chitooligosaccharides, an oligomer form of chitin. The smallest oligomer substrate was an N-acetylglucosamine tetramer. These results suggested that this enzyme was an endo-splitting chitinase with short substrate cleavage activity and useful for biotechnological applications, in particular for the production of N-acetyl chitooligosaccharides. 相似文献