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1.
Summary An unstable long tandem duplication which includes the white locus twice, marked with w sp in the left and w 17G in the right locus, when kept in males has been found to produce red-eyed sons which have lost the long duplication and with it the w sp and w 17G mutants. Such exceptions were produced also when w 17G had been exchanged for w a.Stocks originating from these exceptions are unstable, producing: 1) zeste males, also unstable, 2) w - deletions, stable, 3) transpositions of the white locus to sites in other chromosomes.The instability is interpreted as the effect of an IS element, within or adjacent to the white locus, which is supposed to retain a duplication of the proximal zeste interacting part of this locus. According to the orientation of the IS element the duplicated part can be active or inactive, giving a zeste or red eye phenotype.The frequency of exceptional offspring after X-ray treatment of the red and zeste unstable stocks have been compared to stable stocks with corresponding genotypes.  相似文献   

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Integration of a plasmid carrying the TnBP3 transposon of Bordetella pertussisinto the chromosome of Escherichia coliand transpositions of the integrated structure within a chromosome in the wild-type and mutant cells ptsHdevoid of the major Hpr protein of the phosphoenolpyruvate-dependent phosphotransferase system were studied. When transposed to a new chromosome site, the integrated structure was precisely (or almost precisely) excised from the metYgene sequence, which resulted in restoration of the Met+phenotype. The integration and transposition events were only observed in the E. colicells carrying the ptsH +allele. The ptsHmutations inhibited integration and intramolecular transposition, which were restored after phenotypic or genetic suppression of the ptsHmutation. The intensity of the processes studied were suggested to depend on the integrity of a chain that ensures transferring of the phosphoryl residue by proteins of the phosphotransferase system in E. coliK12.  相似文献   

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A member of Ising's family of large transposing elements (TEs) has inserted into, or very near, the crinkled (ck, 2–50) locus. This TE (TE36) carries functional alleles of both the white and roughest loci, and causes a hypomorphic mutation of ck. The TE is visible in polytene chromosomes as a two-banded insertion between 35B9 and 35C1. These bands show homology to foldback (FB) elements by in situ hybridization. All spontaneous losses of TE36 remain mutant for ck and retain sequences homologous to FB at the site of TE's insertion. TE36 carries only one functional copy of w +, by the criterion that z w, TE36/ + flies are wild-type for eye color but z w; TE36/TE36 flies are zeste. This white+ gene is dosage compensated since w/Y; TE36/+ males have twice as much eye pigment as w/w; TE36/ + females. A form of the TE that has four polytene chromosome bands and expresses twice as much pigment as TE36 has been recovered. However, its white genes are not suppressed by zeste.  相似文献   

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Gubb D  Roote J  McGill S  Shelton M  Ashburner M 《Genetics》1986,112(3):551-575
TE146, a large transposing element of Drosophila melanogaster, carries two copies of the white and roughest genes in tandem. In consequence, z1 w 11E4; TE146(Z)/+ flies have a zeste (lemon-yellow) eye color. However, one in 103 TE146 chromosomes mutates to a red-eyed form. The majority of these "spontaneous red" (SR) derivatives of TE146 have only one copy of the white gene and are, cytologically, two- to three-banded elements, rather than six-banded as their progenitor. The SR forms of TE146 are also unstable and give zeste-colored forms with a frequency of about one in 104. One such "spontaneous zeste" (SZ) derivative carries duplicated white genes as an inverted, rather than a tandem, repeat. The genetic instability of this inverted repeat form of TE146 is different from that of the original tandem repeat form. In particular, the inverted repeat form frequently produces derivatives with internal rearrangements of the TE and gives a much lower frequency of SR forms. In addition, two novel features of the interaction between w+ alleles in a zeste background have been found. First, copies of w + can become insensitive to suppression by zeste even when paired. Second, an inversion breakpoint may disrupt the pairing between two adjacent w+ alleles, necessary for their suppression by zeste, without physically separating them.  相似文献   

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Effective transposon tagging with theAc/Ds system in heterologous plant species relies on the accomplishment of a potentially high transposon-induced mutation frequency. The primary parameters that determine the mutation frequency include the transposition frequency and the transposition distance. In addition, the development of a generally applicable transposon tagging strategy requires predictable transposition behaviour. We systematically analysedDs transposition frequencies andDs transposition distances in tobacco. An artificialDs element was engineered with reporter genes that allowed transposon excision and integration to be monitored visually. To analyse the variability ofDs transposition between different tobacco lines, eight single copy T-DNA transformants were selected. Fortrans-activation of theDs elements, differentAc lines were used carrying an unmodifiedAc + element, an immobilizedsAc element and a stableAc element under the control of a heterologous chalcone synthas (chsA) promoter. With allAc elements, eachDs line showed characteristic and heritable variegation patterns at the seedling level. SimilarDs line-specificity was observed for the frequency by whichDs transpositions were germinally transmitted, as well as for the distances of theDs transpositions. ThesAc element induced transposition ofDs late in plant development, resulting in low germinal transposition frequencies (0.37%) and high incidences of independent transposition (83%). The majority of theseDs elements (58%) transposed to genetically closed linked sites (10 cM).  相似文献   

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Summary ADrosophila transposable element, the P element, containing the bacterial gene encoding -galactosi-dase is widely used to search for tissue-specific enhancers. Thislac-Z-containing P element (P-lacZ ry +) can be moved around the genome by a number of techniques. When it comes to lie close to a tissue-specific enhancer, blue staining results in particular tissues of the fly. Many different patterns of expression have already been obtained with the long-term aim of cloning the nearby genes that these enhancers normally regulate. Whilst analysing a set of flies containing theseP-lacZ inserts for sex-specific expression in the adult, a preference was noticed for insertion into regions of DNA generating expression of -galactosidase in the male gonad. Since the transposition events generating these flies occurred in the male germ-line, it seemed possible that there was preferential insertion of the element into DNA which was being transcribed. To test this, transpositions were generated of the sameP-lacZ ry + in both the male and female germ-lines. The results are compatible with the above hypothesis. This finding has important implications for the type of enhancers likely to be found by this method, and may also be relevant to those using P elements as mutagens in Drosophila and for the study of the mechanism of P transposition.  相似文献   

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Nuzhdin SV 《Genetica》1999,107(1-3):129-137
Transposable elements (TEs) are sequences capable of multiplying in their host's genome. They survive by increasing copy numbers due to transpositions, and natural selection washes them out because hosts with heavier loads of TEs have lower fitness. The available phylogenetic evidence supports the view that TEs have existed in living organisms for hundreds of millions of years. A fundamental question facing the field is how can an equilibrium be attained between transposition and selection which allows these parasitic genetic elements to persist for such a long time period? To answer this question, it is necessary to understand how the rate of TE transposition is controlled and to describe the mechanisms with which natural selection opposes TE accumulation. Perhaps the best models for such a study are copia and gypsy retrotransposons in Drosophila. Their average rate of transposition in nature is between 10?5 ? 10?4 transpositions per copy per generation. Unlike nature, transposition rates vary widely, from zero to 10?2, between laboratory lines. This variability in transposition rate is controlled by host genes. It is probable that in nature TE site heterogeneity is caused by frequent transpositions in rare flies with permissive alleles, and no transpositions happen in the rest of flies. The average rate of TE transposition in nature may thus depend on the frequency of permissive alleles, which is a function of the rate of mutation from restrictive to permissive alleles, the mechanism and the strength of selection opposing TE multiplication, and population size. Thus, evolution of the frequency of permissive alleles of genes controlling transposition must be accounted for to understand evolution of TE copy numbers.  相似文献   

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The phenotypic effects of different doses of the dominant, sex-linked mutant Notch (N) and its wildtype allele (N +) were studied in Drosophila hydei, N being lethal in homozygous or hemizygous condition. Various dosage combinations were made by using N + N and N + N + attached-X chromosomes as well as X and Y N +-duplication chromosomes (w mCoY, XwmCo,and DpCo Nt). The N mutant used, N 68, is associated with a small inversion: In (I) N 68.The wing phenotype was found to depend solely on the number of functional (N +) alleles present, irrespective of the dose of N. Females with a single dose of N + are phenotypically Notch, females with three or four doses of N + show a Confluens wing phenotype. The latter occurs in varying degrees of expression which seem to be correlated with the relative amounts of sex-chromosomal heterochromatin present. In males the N + locus behaves as a dosage compensated locus either on the X or the Y chromosome.In the w mCo (w+N+) duplication, the w + locus shows variegation when placed over white, whereas N + placed over N 68 does not. The former being situated closer to the heterochromatin in this aberration, this is consistent with the idea of gene inhibition by heterochromatin but at the same time would imply a very limited spreading effect.  相似文献   

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Summary Females heterozygous for the two alleles dnc 2 and dnc M14 of the X-linked gene dunce (dnc), and carrying a copy of dnc + progeny X-chromosomes from recombination experiments. Restriction site polymorphisms have been used as genetic markers to follow the parentage of dnc locus segments in these chromosomes. All six chromosomes are identical with respect to the spectrum of restriction site markers they carry in the dnc + chromosomal region. In the progeny chromosomes, this region is comprised of sequences like the dnc M14 X-chromosome and the translocation copy of dnc +. Sequences flanking the dnc gene in the progeny chromosomes are like the dnc M14 chromosome. Internal to the gene but near the 5 end, is a segment from the dnc + translocation which has apparently originated from an interchromosomal and premeiotic gene conversion event. In addition, two transposable elements have inserted into the progeny chromosomes, one towards the 5 end of dnc and the other near the 3 end. The insertion of these elements occurred premeiotically since all six chromosomes are structurally identical. The data are interpreted with respect to a potential role of transposable element transposition in the process of gene conversion.  相似文献   

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Chromosomes and phenotypes of four different sex-linkedwhite-mottled mutants of the position-effect variogation type were studied. Three mutants (w m1,w m2,w m3) are X-chromosomal rearrangements which shift the w+ locus into a position close to heterochromatin, but which have different ouchromatic and heterochromatic breaks. The fourth, a spontaneous derivative ofw m1, is an insertional duplication of part of the X chromosome, including thew + andN +loci. The duplicated segment is inserted into the distal part of the long arm of the heterochromatic Y chromosome. It is designated,w m CoY, orXw m Co when transferred to the X chromosome.Three chromosomal types (w m1,w m CoY) and (Xw m Co) having the same cuchromatic break near thew + locus, cause large-spotted eyes whereas two others (w m2,w m3) produce a popper-and-salt type of mottling. From the position of the various eu- and heterochromatic breaks, it appears that the distance of thew + locus to the point of reunion with heterochromatin, rather than the amount or type of adjoining heterochromatin, dietates the phenotypic action of the displacedw + locus, in the sense of a spreading effect on two proposed functional subunits within thew + locus.The pigmentation background against which the mottling effect is produced, i.e., a givenw-allele with its characteristic colour, or other eye colour mutations, does not seem to affect the type of mottling. Drosopterins and ommochromes react in the same way to modifing factors like temperature and supernumerary Y chromosomes. Two mutants (w m2 andw m CoY) while reacting in the same manner to Y chromosomes showed an opposite temperature response.By exchange between the heterochromatin of the Y and X chromosome inw/w m CoY males thew m Co duplication was transferred between the sex chromosomes with a certain regularity. It is not yet known wether the exchanges are mitotic or meiotic in origin but their heterochromatic nature has been demonstrated cytologically.  相似文献   

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Summary By classical genetic experiments, evidence is provided that the male recombination factor, 31.1 MRF, has the ability to be transposed to another chromosome. The procedure by which the transposition occurs must be different from that of classical crossing over. It appears that transposition occurs only when the factor is active in male germ cells. Moreover, the factor appears to be able to undergo successive transpositions. Furthermore, the integration sites of the factor, when transposed into another chromosome, may not be completely random. Finally, the third chromosome of the 31.1/Cy L 4 strain can also induce male recombination.  相似文献   

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A ring-Y chromosome, R(Y)w m, of D. hydei is described which carries a complete set of fertility genes, a NOR region and a small X-chromosomal insertion (w m), which may be used as a marker. The ring has been characterized by various staining techniques. It was derived from a w mCo Y chromosome by X-ray treatment of spermatocytes. Its mode of origin allows to fix the gene order in the distal region of the long arm of the w mCoY chromosome. The white + gene included in the ring shows a new type of position-effect variegation which is described and discussed in the context of an earlier hypothesis on a dual function of the white locus.  相似文献   

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Two recombinant mice have been recovered from the progeny of Ttf/t w32+ animals. They have lost the tw32 lethality factor(s) and gained tufted, presumably from the T chromosome. Southern blot analysis of class I genes of these two new partial t PA027 and t PA286 haplotypes indicates that they have retained at least part of the major histocompatibility complex of the t w32 chromosome (H-2 haplotype H-2 w28). We have prepared a phage library of Eco RI-digested DNA from homozygous t PA027 animals. Upon screening the library with a cDNA probe specific for H-2K genes, we isolated a class I gene displaying all of the distinctive features of a genuine H-2K gene, and which could thus be defined as an H-2K w28 gene. The H-2K w28 gene is 92–95% homologous to H-2K band H-2K dgenes and differs significantly from the other class I genes sequenced so far. Homology with the H-2K bsequence reaches nearly 100% in the 3 part of the H-2K w28 gene. Moreover, the homology with an H-2K qcDNA sequence reaches 99.8%. Several hypotheses can account for the near identity of H-2K b, H-2K q,and H-2K w28 gene sequences: either recombination between H-2 w28 and H-2 band H-2 qsequences occurred before or at the.time the strain was established, or the class I genes of the t w32 chromosome and the H-2 band H-2 qgenes found in inbred strains of mice have separated from each other rather recently.  相似文献   

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Foldback (FB) elements are transposable elements found in many eukaryotic genomes; they are thought to contribute significantly to genome plasticity. In Drosophila melanogaster, FBs have been shown to be involved in the transposition of large chromosomal regions and in the genetic instability of some alleles of the white gene. In this report we show that FB mediated transposition of w 67C23, a mutation that deletes the promoter of the white gene and its first exon, containing the start codon, can restore expression of the white gene. We have characterized three independent events in which a 14-kb fragment from the w 67C23 locus was transposed into an intron region in three different genes. In each case a local promoter drives the expression of white, producing a chimeric mRNA. These findings suggest that, on an evolutionary timescale, FB elements may contribute to the creation of new genes via exon shuffling.Electronic Supplementary Material Supplementary material is available in the online version of this article at Communicated by G. P. Georgiev  相似文献   

19.
A DNA fragment containing a Klebsiella oxytoca gene for polygalacturonate trans-eliminase was cloned into the kanamycin resistance transposon Tn5. This new transposon, designated Tn5-Pga +, had a transposition frequency of 1×10-6. The broad host range plasmid pR751::Tn5-Pga + was conjugally transferred to a variety of genetic backgrounds. The ability to degrade polygalacturonate was expressed in Aeromonas hydrophila, Alcaligenes eutrophus, Azotomonas insolita, Escherichia coli, Pseudomonas putida and Rhodopseudomonas sphaeroides, but not in Zymomonas mobilis.Abbreviations PGA polygalacturonate - UGA unsaturated galacturonic acid - PATE polygalacturonate trans-eliminase - PG polygalacturonase - CVP crystal-violet pectate  相似文献   

20.
TE146 is a transposing element (TE) consisting of six polytene chromosome bands that has inserted into the no-ocelli (noc 250) locus. This member of Ising's TE family carries two copies of the white and roughest loci. TE146 is lost from noc with a spontaneous frequency of approximately 1 in 22000 chromosomes. All spontaneous losses are accompanied by the reversion of the noc mutation associated with the TE. The TE is associated with fold-back (FB) sequences. The losses of TE146 retain fold-back homology at noc. Of 26 -ray-induced losses of TE146, 16 are gross deletions, removing loci neighboring noc and ten are not. The non-deleted -ray-induced losses are either noc and rst + or noc + and rst . The white+ genes of TE146 are dosage compensated since w/Y; TE146/+ and w/w; TE146/+ flies are sexually dimorphic for eye color. These w + genes are also suppressed by zeste since z w; TE146/+ flies have zeste-colored eyes.  相似文献   

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