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1.
《Biotechnic & histochemistry》2013,88(4):122-130
The recent discovery that the actual staining agent in the Ziehl-Neelson technic is an addition product of the phenol and the dye employed led the authors to investigate the character of the reaction products of various basic dyes with a considerable variety of cyclic derivatives of a phenolic or acid character. Analytical data are presented which indicate that basic dyes form addition products, in general, with typical phenols. With more definitely acid cyclic derivatives the reaction is primarily metathetical, resulting in the formation of organic salts of the dyes. In some instances both metathesis and addition result. Readers are referred to the following paper for information as to the practical staining value of certain of these compounds. 相似文献
2.
《Biotechnic & histochemistry》2013,88(3):98-102
The chemical theory of staining is discussed in the light of recent discoveries respecting the mechanism of the reaction of basic dyes with substances of an acid character. It is pointed out that staining may result through the formation of addition products as well as through the formation of dye salts. 相似文献
3.
Walter C. Holmes 《Biotechnic & histochemistry》1926,1(3):98-102
The chemical theory of staining is discussed in the light of recent discoveries respecting the mechanism of the reaction of basic dyes with substances of an acid character. It is pointed out that staining may result through the formation of addition products as well as through the formation of dye salts. 相似文献
4.
Holde Puchtler Susan N. Meloan Barbara R. Brewton 《Histochemistry and cell biology》1975,41(3):185-194
Summary The nature of products formed by aldehydes and Schiff's reagent, whether they are sulfonic or sulfinic acid compounds, has been the subject of much discussion. It seems therefore timely to review early studies of aldehyde-Schiff reactions, including the history of pararosanilin and related dyes. Dyes of the basic fuchsin group have been studied extensively since 1862, and their triphenylmethane structure was established in 1878. The currently used structural formulas were introduced around the turn of the century. Reactions of basic fuchsin with aldehydes, with and without addition of SO2, were investigated by Schiff in the 1860's i.e. before the structure of these dyes was known. In 1900 Prud'homme showed that the reaction products of basic fuchsin, sodium bisulfite and formaldehyde are alkylated and sulfonated derivatives of the parent compound; further chemical studies indicated attachment of the sulfonic acid group to the carbon atom of the aldehyde. Prud'homme's findings were repeatedly confirmed during the following decades. Wieland and Scheuing were apparently unaware of these studies and introduced the sulfinic acid theory in 1921; furthermore, they considered substitution at two amino group of Schiff's reagent essential for formation of the colored compound. However, later chemical and spectroscopic studies showed no evidence of-N-sulfinic acids but supported the sulfonic acid theory of Prud'homme. 相似文献
5.
Synopsis An investigation of the role of phosphotungstic and phosphomolybdic acids in Mallory-like trichrome methods showed unexpectedly that, rather than acting as mordants to anionic dyes, these polyacids selectively blocked staining of all tissue components other than connective tissue fibres to Aniline Blue and other similar fibrereactive dyes. Connective tissue components were found to contain residues resembling histidine that are easily accessible to anionic dyes. Blocking towards typical anionic dyes for demonstrating plasma proteins, such as Biebrich Scarlet, was also demonstrated but was less complete. The blockade of both types of dye was labile if the staining times were extended; plasma dyes were more sensitive than fibre dyes in this respect. Histochemical reactions for tyrosine residues were blocked. In connective tissue, phosphotungstic acid did not block histidine residues demonstrable by the coupled tetrazonium reaction with previous iodination. Thus it is postulated that differential trichrome staining occurs by binding of Aniline Blue to basic residues in the connective tissue not blocked by phosphotungstic acid and subsequent replacement of the blocking agent by an anionic dye. The binding of phosphotungstic acid to both epithelium and connective tissue was demonstrated by the quenching of autofluorescence in these regions and by the reduction of the bound PTA to blue coloured products with titanium trichloride. 相似文献
6.
1. Solutions containing NH4OH and NaOH, and CO2 and HCl may be used to produce various combinations of extracellular and intracellular reactions in starfish eggs, Gonionemus, and Nitella cells. 2. Staining by basic dyes is, with a constant intracellular reaction, favored by increased extracellular alkalinity. With a constant extracellular reaction, staining is hindered by increased intracellular alkalinity. 3. These facts are in opposition to the view that staining of cells by basic dyes is chiefly governed by a combination of the dyes with cell proteins. It is more in harmony with the view that the combination is with a substance or substances of acid nature. 相似文献
7.
Summary. Sorbic acid has a system of conjugated double bonds which makes it able to undergo nucleophilic addition reactions with certain
functions. The interactions between sorbic acid and an amine present in the endogenous constituents of food were quantified.
We demonstrated the formation of new products and studied the underlying mechanisms using ethyl sorbate and various amines.
HPLC, GC, GC-SM and NMR analyses of the reaction mixes enabled the isolation and identification of the products. The addition
reactions led, at 20°C, to linear monoadducts and, at 50°C, to cyclic derivatives resulting from double addition. Mutagenesis
studies, involving Ames test and genotoxicity studies with HeLa cells and on plasmid DNA, in cyclic interaction products,
showed that none of the products studied presented neither mutagenic nor genotoxic activities.
Received September 28, 1999 相似文献
8.
A. T. Sumner 《The Histochemical journal》1982,14(2):283-299
Summary The effect of cisplatin (cis-dichloro-diamminoplatinum II) treatment on staining of nuclei with various basic dyes and with the Feulgen reaction has been studied. Although cisplatin is reported to show negligible reaction with DNA phosphates, it has a substantial blocking effect on staining with most dyes. Short treatment with cisplatin results in binding mainly to guanine bases of DNA, causing partial blocking of the Feulgen reaction and almost complete blocking of ethidium intercalation; binding of neutral red and crystal violet is enhanced, apparently as a result of cisplatin-induced denaturation of DNA. Very prolonged cisplatin treatment does not completely block the Feulgen reaction, indicating that reaction of cisplatin with purine bases is not complete. Since attachment of cisplatin to DNA bases is unlikely to prevent binding of most basic dyes, it is suggested that the blocking of their staining may result from steric hindrance caused by formation of DNA-protein cross-links by cisplatin. Whatever the mechanism, it is incapable of producing complete blocking of staining with certain dyes. As a practical tool, it appears that rapid and almost complete blocking of staining by cisplatin may be used as an indicator of intercalative binding of dyes to DNA. 相似文献
9.
We can divide metachrome mordant staining of nuclei after graded 60 degrees C 1 N nitric acid extraction into three groups. The Feulgen nucleal reaction and dilute cationic dye staining of nuclei are abolished in about 30 minutes. With one group of metachrome dyes nuclear staining is lost with acid exposures of one hour or less. In a second group nuclear staining is weakened by 30-60 minute extractions, but persists in recognizable grade for 4-6 hours. In the third group nuclear staining remains almost unimpaired for 4-6 hours. In the first group the nuclear staining seems clearly assignable to the nucleic acids and to DNA in particular. In the second group loss of part of the reactivity on short exposure indicates some participation of DNA in the control staining result, as well as participation of basic nucleoprotein. In the third group staining seems assignable largely to basic nucleoprotein. The five gallocyanin group dyes, all in group 1, all possess a dialkylamino group, probably functioning as an ammonium chloride.Hematoxylin, the flurone blacks and gallein all present an o-hydroxysemiquinone group which probably acts as a weak acid, in addition to the carboxyl group of gallein which gives the strongest staining of nuclei at the longest acid exposure. Deoxyribonuclease digestion (2 hours, 37 degrees C) separated sharply a class in which nuclear staining failed completely, a class in which nuclear staining was fully equal to that in the control preparations and an intermediate group in which slight, moderate, or severa impairment was present. Generally there was good agreement between the two methods of nucleic acid removal, despite the fixation difference. In each case, however, the extraction procedure was one worked out for the fixation on which it was used. 相似文献
10.
Kiyoshi Harada 《Biotechnic & histochemistry》1976,51(5):255-260
Phenol is not essential to acid-fast staining, for it will occur in the absence of phenol where such lipoid-soluble basic dyes as night blue, Victoria blue B or Victoria R are used; it is essential for acid-fast staining with water soluble basic dyes such as basic fuchsin. When phenol is added to the staining solution, such water soluble basic dyes behave in effect like their lipid-soluble counterparts. The loss of mycobacterial acid-fastness with carbolfuchsin after bromination or chromation indicates that this phenomenon is related to the presence of unsaturated lipids in the bacterial cells. Within the cells these acid-fast lipids are bound in such a way that they are easily removed from all mycobacteria by hot dilute HCl; from leprosy bacilli alone they are easily removed with hot pyridine. From the results of various blocking reactions it appears that carboxyl and especially hydroxyl groups of these cellular lipids are essential to the acid-fast reaction of mycobacteria. 相似文献
11.
K Harada 《Stain technology》1976,51(5):255-260
Phenol is not essential to acid-fast staining, for it will occur in the absence of phenol where such lipoid-soluble basic dyes as night blue, Victoria blue B or Victoria R are used; it is essential for acid-fast staining with water soluble basic dyes such as basic fuchsin. When phenol is added to the staining solution, such water soluble basic dyes behave in effect like their lipid-soluble counterparts. The loss of mycobacterial acid-fastness with carbol-fuchsin after bromination or chromation indicates that this phenomenon is related to the presence of unsaturated lipids in the bacterial cells. Within the cells these acid-fast lipids are bound in such a way that they are easily removed from all mycobacteria by hot dilute HCl; from leprosy bacilli alone they are easily removed with hot pyridine. From the results of various blocking reactions it appears that carboxyl and especially hydroxyl groups of these cellular lipids are essential to the acid-fast reaction of mycobacteria. 相似文献
12.
Krieg R Eitner A Günther W Schürer C Lindenau J Halbhuber KJ 《Journal of molecular histology》2008,39(2):169-191
Fluorescent labeling of immuno-bound or endogenous peroxidase (PO) activity has been achieved to date by means of phenol derivatives with a low substitution degree. Here it is demonstrated that N,N-dialkylamino-styryl dyes can also act as fluorescent substrates of PO. They undergo enzymatically cross-linking reactions to surrounding cell constituents in an analogous manner thus permitting highly fluorescent and permanent labeling. This approach is narrowly related to the catalyzed reporter deposition (CARD) technique based on tyramine conjugates and the recently described catalytic cross-linking approach of hydroxystyryl derivatives. The substitution patterns for optimal cross-linking capability and the spectral properties of obtained specific reaction products were studied using an iterative semi-empirical approach. The best staining performance is achieved with N,N-dimethylaminoaryl derivatives. Their N,N-dialkyl homologues as well as the primary aryl amine pendants failed as PO substrates. Due to their basic character, novel substrates occasionally tend to unspecific interactions (staining nuclei, mast cells, or keratin). Centering this side specificity and repressing the staining capability of PO was achieved by chemical modification of the respective dye leading to new specific probes for keratin and cytoplasmatic RNA. In conclusion, catalytic cross-linking of heterocyclic 4-N,N-dimethylamino-styryl dyes represents a promising approach for the permanent fluorescent staining of PO in fixed cells and tissues, complementing the CARD technique. In contrast to CARD-related approaches, new substrates are characterized by a broad excitation and emission range of fluorescence and the outstanding spatial resolution of specific fluorescence signaling known so far from their 4-hydroxystyryl analogues. They currently represent the smallest fluorescent substrates of PO. Histochemical and immuno-histochemical applications share several outstanding features: High detection sensitivity, spatial resolution of fluorescence signaling, and photo stability. 4-N,N-dimethylamino-styryl substrates are compatible with their phenol and phenol-ester analogues. Their combination facilitates the trichromatic immuno-histochemical demonstration of three different targets simultaneously at one excitation wavelength in a conventional epi-fluorescence microscope. 相似文献
13.
C. J. Cooksey 《Biotechnic & histochemistry》2019,94(2):100-107
Three groups of insect dyes are described: three cochineal dyes, the kermes dye and the lac dye. The major color components are carminic acid, kermesic acid and laccaic acids, respectively. These dyes are red anthraquinone derivatives. The chemical structures are described. All of these dyes have extensive histories that are related briefly; however, only American cochineal is of commercial importance today. Two manufactured derivatives of cochineal, carmine and acid-stable carmine (4-aminocarminic acid) are described in some detail including the chemical identity, toxicity, stability, and staining and non-staining applications. 相似文献
14.
M Titford 《Biotechnic & histochemistry》2001,76(1):23-30
Seventeen Grübler dyes produced in Germany between 1880 and 1939 were examined in this study. These dyes were: fuchsin-bacillus, diamond fuchsin, fuchsin S acid, rubin S, safranin O water soluble, safranin yellowish water soluble, methyl eosin, Sudan III, scarlet R, auramine, orange G, aniline blue, pyronin, carmine, lithium carmine, hematein and aurantia. Spectrophotometry and staining characteristics were used to determine the maximum absorbance and efficacy of each dye in common staining techniques. The spectral curves and staining characteristics of these dyes compared well with modern dyes used as controls. Fuchsin bacillus and diamond fuchsin are synonyms for basic fuchsin. Fuchsin S acid and rubin S are synonyms for acid fuchsin. The scarlet R sample was the same as the Sudan III. The two safranins were the same. The basic fuchsin samples were unsuitable for preparation of Schiff's reagent. Both basic fuchsin and pyronin samples were less concentrated than modern counterparts. It is noteworthy that the dyes worked well after up to 100 years in storage, and this observation indicates that dyes can have a long shelf life when stored in cool, dry, air-tight conditions. 相似文献
15.
Summary We can divide metachrome mordant staining of nuclei after graded 60°C 1N nitric acid extraction into three groups. The feulgen
nucleal reaction and dilute cationic dye staining of nuclei are abolished in about 30 minutes. With one group of metachrome
dyes nuclear staining is lost with acid exposures of one hour or less. In a second group nuclear staining is weakened by 30–60
minute extractions, but persists in recognizable grade for 4–6 hours. In the third group nuclear staining remains almost unimpaired
for 4–6 hours.
In the first group the nuclear staining seems clearly assignable to the nucleic acids and to DNA in particular. In the second
group loss of part of the reactivity on short exposure indicates some participation of DNA in the control staining result,
as well as participation of basic nucleoprotein. In the third group staining seems assignable largely to basic nucleoprotein.
The five gallocyanin group dyes, all in group1, all possess a dialkylamino group, probably functioning as an ammonium chloride. Hematoxylin, the fluorone blacks and gallein
all present ano-hydroxysemiquinone group which probably acts as a weak acid, in addition to the carboxyl group of gallein which gives the
strongest staining of nuclei at the longest acid exposure.
Dexyribonuclease digestion (2 hours, 37°C) separated sharply a class in which nuclear staining failed completely, a class
in which nuclear staining was fully equal to that in the control preparations and an intermediate group in which slight, moderate,
or severa impairment was present. Generally there was good agreement between the two methods of nucleic acid removal, despite
the fixation difference. In each case, however, the extraction procedure was one worked out for the fixation on which it was
used.
Assisted by National Cancer Institute Contract No. NO 1 CB43912. 相似文献
16.
P. R. Lewis 《Histochemistry and cell biology》1962,2(6):423-426
Summary A new buffer system is described for use in histochemical staining with basic dyes. The buffer is made up by mixing solutions of formic acid and sodium acetate. Tables giving the proportions for closely-spaced pH values in the range 3.0–5.6 are presented. A table for an acid phosphate series down to a pH of 2.0 is also included. The value of these buffer mixtures in histological as well as histochemical staining with basic dyes is stressed.With 1 Figure in the Text 相似文献
17.
Kiyoshi Harada 《Histochemistry and cell biology》1967,10(1):74-87
Summary Oxidation and bromination of mouse kidney JG cell-granules result in the production of cysteic acid from cystine; cysteic acid is capable of taking up rapidly and selectively certain basic triphenyl methane dyes including aldehyde fuchsin at lower pH levels.After treatment with periodic acid, bromine and hydrochloric acid, the JG granules or the nuclear chromatin also take up the basic triphenyl methane dyes (including aldehyde fuchsin) which contain amino groups, probable as a result of the production of aldehyde groups. Basic triphenyl methane lacking amino groups does not react with aldehydes.Some substance present in JG granules could be stained by aldehyde fuchsin after prior oxidation; HCl methyl violet 2B was taken up both with or without prior oxidation. Only strong methylation completely abolished these affinities which were restored after demethylation. These reactions are attributed to cystine.The staining of JG granules with dilute aldehyde fuchsin and dilute methyl violet 2B is not affected by oxidation, bromination, aldehyde blocking and hydrolysis; these reactions are abolished by mild methylation, but restored by subsequent saponification. These staining properties are due to the presence of carboxylic acid in JG granules.The positive PAS reaction of JG granules is due to the presence of 1.2-glycol in the same granules. 相似文献
18.
Bacterial cells were stained in sequence, and at various pH's, by 22 different basic dyes. It was found that any dye could replace another already present in the bacterial cell. This replacement was shown to act according to mass action laws for reversible reactions, and hence was influenced by concentration of reagent and time of application. Since basic dyes are also known to react at carboxyl group sites, the phenomena of staining of bacterial cells by ordinary basic dyes must be a chemical adsorption exchange reaction. 相似文献
19.
《Biotechnic & histochemistry》2013,88(4):181-182
Bacterial cells were stained in sequence, and at various pH's, by 22 different basic dyes. It was found that any dye could replace another already present in the bacterial cell. This replacement was shown to act according to mass action laws for reversible reactions, and hence was influenced by concentration of reagent and time of application. Since basic dyes are also known to react at carboxyl group sites, the phenomena of staining of bacterial cells by ordinary basic dyes must be a chemical adsorption exchange reaction. 相似文献
20.
Summary For small basic dyes blocking or selective extraction of tissue sections can reduce staining (methylation, or trichloracetio acid extraction) or increase staining (acetylation or nitrous acid deamination). This is as predicted by a simple electrostatic model of dye binding. In contrast, the staining with both large basic dyes and with metal complex dyes was only slightly affected by these treatments. This is in keeping with the probable role of van der Waals attractions and hydrophobic bonding in the binding of large dyes.Since MCDs are all large, there is no need to invoke the traditional, special mechanism of mordanting (i.e. tissue-metal ion-dye covalencies) to explain the peculiarities of these MCDs. An apparent exception to this was the resistance of MCDs, unlike either small or large basic dyes, to the action of processing fluids. However, this was due to the insolubility of MCDs in alcoho, lsin contrast to the solubility of most basic dyes, irrespective of size, in these media. 相似文献