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M Matumoto 《Microbiological reviews》1966,30(1):152-176
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Development of antibody to measles virus polypeptides during complicated and uncomplicated measles virus infections. 总被引:6,自引:3,他引:3
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M Graves D E Griffin R T Johnson R L Hirsch I L de Soriano S Roedenbeck A Vaisberg 《Journal of virology》1984,49(2):409-412
Immune precipitation of 181 sera from 152 patients with natural measles was studied to determine the temporal course and frequency of antibody responses to nucleocapsid, fusion, hemagglutinin, and matrix proteins of measles virus. Large amounts of antibody to nucleocapsid protein developed in all patients by day one of the rash. Antibody to hemagglutinin and fusion proteins developed in all patients over the next 3 weeks, the former to high levels and the latter to low levels. Antibody to matrix protein developed to very low levels and was detectable in only 41% of the patients; this poor response to matrix protein was not correlated with the age of the patient or the acute neurological complications of measles. 相似文献
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Polyadenylated mRNA extracted from cytoplasm of measles virus-infected Vero cells was translated in a cell-free system. Three of the polypeptides obtained corresponded to nucleocapsid protein, phosphoprotein, and membrane protein of measles virions. A fourth polypeptide, present in measles virus-infected cells, could be generated by addition of Vero cytoplasmic extract and was identified as a cleavage product of the nucleocapsid protein. 相似文献
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Efficient propagation of measles virus in suspension cultures. 总被引:1,自引:1,他引:0
Suspension cultures of a human prostate cell line (MA160) supported abundant growth of the Edmonston strain of measles virus. The virus yields obtained with these suspension cultures (150 to 800 PFU/cell) were at least 20- to 100-fold higher than those frequently reported in the literature. Monolayer cultures of MA160 cells did not support a virus replication nearly as efficiently (progeny yield, 25 PFU/cell). 相似文献
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Infectious measles virus from cloned cDNA. 总被引:12,自引:1,他引:11
I Ballart D Eschle R Cattaneo A Schmid M Metzler J Chan S Pifko-Hirst S A Udem M A Billeter 《The EMBO journal》1990,9(2):379-384
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V. M. Zhdanov 《Molecular and cellular biochemistry》1980,29(1):59-66
Summary Measles is one of widely spread virus infections that is a major cause of deaths in some tropical areas. The measles virus is a member of the genus of Morbillivirus of the family of Paramyxoviridae. The virions contain six polypeptides, including one glycoprotein; two of them are surface proteins that possess hemagglutinating and hemolytic activities, one of them is polymerase. Replication of the measles virus is similar to that of other Paramyxoviruses. Besides the acute infection for measles virus a persistent infection is characteristic that affects central nervous system and inner organs. Molecular mechanisms of it were studied and the results are discussed to explain the pathogenesis of subacute sclerosing panencephalitis, systemic lupus erythematosus and other diseasis in which measles or measles-like virus may be involved. 相似文献
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Two sets of independently isolated measles virus temperature-sensitive mutants were quantitatively tested for complementation. Analysis of the nine possible combinations of representative mutants indicated that only one pair of mutants is noncomplementing. Thus, the measles virus mutants studied to date define five complementation groups. 相似文献
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We have obtained a polyclonal antiserum, N-BE, against the denatured, amino-terminal half of the measles virus (MV) nucleocapsid (N) protein and a monoclonal antibody (MAb), N46, which recognizes a conformation-dependent epitope in the same region. Amino acid residues 23 to 239 were required and sufficient for the formation of the conformational epitope. Using these antibodies, we show that the N protein of MV is synthesized as a relatively unfolded protein which first appears in the free-protein pool. This nascent N protein undergoes a conformational change into a more folded mature form. This change does not require the participation of other viral proteins or genomic RNA. The mature N protein does not accumulate in the free-protein pool but is quickly and selectively incorporated into the viral nucleocapsids. The mature N protein is a target for interaction with the phosphoprotein (P protein) of MV. This interaction interferes with the recognition of the N protein by the N46 MAb. This suggests that the association with the P protein may mask the binding site for the N46 MAb or that it induces a conformational change in the N protein. 相似文献
10.
Y Yoshikawa K Yamanouchi T A Sato S Katow M Ito 《Japanese journal of medical science & biology》1975,28(2):117-126
The Niigata-1 strain isolated from a patient with subacute sclerosing panencephalitis (SSPE) was inoculated intracerebrally into ferrets. Neurological signs developed in about 1 week in most of the animals. Histopathological examinations of the central nervous tissues revealed degenerative lesions in the parenchyma of the brains and inflammatory reactions predominantly in the meninges and choroid plexus. Virus antigen was demonstrated mainly in the nerve cells by immunofluorescent staining. The results indicated high affinity of the Niigata-1 strain to the nerve cells. In contrast, the Mantooth strain of SSPE virus in cell-free state did not exhibit neurovirulence in ferrets. 相似文献
11.
M Kańtoch W Falecka B Litwińska J Lityńska W Sadowski 《Acta microbiologica Polonica》1977,26(4):429-438
Experiments discussed in this paper aimed at obtaining, using Cercopithecus aethiops monkeys information on immunogenic activity of measles virus as well as kinetic and persistence of immunological reaction at active and inactivated virus. 相似文献
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P R Wyde D K Moore-Poveda N J Daley H Oshitani 《Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)》1999,221(1):53-62
An alternative model to nonhuman primates to study measles virus (MV) pathogenesis, to evaluate potential MV vaccines, or to screen for potential antivirals effective against this virus is highly desirable. The laboratory-adapted Edmonston strain of MV has been reported to replicate in the lungs of hispid cotton rats following intranasal inoculation, immunosuppress infected animals, and disseminate widely from the lungs, making these animals a candidate model. However, clinical MV strains have generally not been found to grow in these animals, limiting the utility and acceptance of this model. In the present studies we demonstrate reproducible replication of several clinical MV strains in hispid cotton rats. As with the Edmonston strain, leukocytes appear to be the primary target cells of these viruses following intranasal inoculation, and extrapulmonary dissemination is common. It is also demonstrated that prior MV infection or immunization of test animals with MV vaccine prevents pulmonary tract infection. These findings should make the MV-cotton rat model more acceptable. 相似文献
14.
《Seminars in Virology》1995,6(6):379-386
Genetic analysis of viruses associated with recent outbreaks of measles in the United States indicated that at least four genotypes were present during 1994 and 1995. None of these more recent genotypes were related to the genotype responsible for the resurgence of measles cases in the United States between 1989 and 1992. The sequence data confirmed that the majority of measles cases that occurred in the United States between 1994 and 1995 were the result of international importation of virus. The data also suggested that transmission of the genotype associated with the resurgence had been interrupted by aggressive control measures. Therefore, molecular epidemiologic studies will provide a powerful means to measure the success of measles control strategies. 相似文献
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Structural components of measles virus 总被引:16,自引:0,他引:16
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The neuraminidase of measles virus 总被引:2,自引:0,他引:2
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G. Huybreghts D.A. Vanden Berghe S.R. Pattyn 《Biochemical and biophysical research communications》1980,93(4):999-1004
Measles virus has been centrifuged on different density gradients. It sediments at densities of 1,20 g/cm3 in K-tartrate, of 1,18–1,21 g/cm3 in sucrose, 1,19–1,23 g/cm3 in CsCl and 1,19 g/cm3 in metrizamide gradients. Metrizamide reduced measles virus infectivity. In sucrose gradients sometimes more than one infectious peak was observed. Control Vero cells produced particles of the same densities as measles virus peaks. These peaks did contain actin as the major protein. The relevance of this finding in relation to the presence of actin in measles virus is discussed. 相似文献
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Neurovirulent TYCSA strain and attenuated Schwarz strain of measles virus and Halle strain of subacute sclerosing panencephalitis (SSPE) virus replicated in cultures of human lymphoid cell lines of the T-cell type, MOLT-3, MOLT-4 and CCRF-CEM. TYCSA and Halle strains grew rapidly, but Schwarz strain grew slowly in these cell lines. Furthermore, these three strains established persistent infection in CCRF-CEM cells but not in the other cell lines. In these persistently infected cultures an almost entire population of cells were shown to be infected and infectious virus was produced constantly for over 100 days. Cells persistently infected with Schwarz strain contained nucleocapsid structures in both the nucleus and cytoplasm and produced low titered infectious virus, whereas nucleocapsid structures were observed only in the cytoplasm of cells persistently infected with either TYCSA or Halle strain and the titers of infectious virus produced from these cells were high. 相似文献